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Citations for New England Biolabs :
1 - 50 of 2032 citations for Pops Par Solution Unlabeled 2000 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... or additional unlabeled ATP (NEB) were added to each reaction as indicated ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 ng of unlabeled crRNA was mixed with equal volume of 2 × RNA loading dye (New England Biolabs) and fractionated in the 12% denaturing polyacrylamide gel ...
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bioRxiv - Biochemistry 2023Quote: ... mouse monoclonal antibodies against PAR (clone 10H, Tulip BioLabs, USA), anti-mouse antibodies conjugated with horseradish peroxidase (Bio-Rad ...
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bioRxiv - Developmental Biology 2021Quote: ... and 2.5 ng/ml BSA (NEB). Samples were incubated at 4°C with rotation for 1 hour and resolved on a 4% polyacrylamide (60:1 ...
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bioRxiv - Biochemistry 2019Quote: ... unlabeled AdoMet was left out and 6 μg of MBP2* protein (NEB) was added to each reaction to increase molecular crowding.
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bioRxiv - Molecular Biology 2023Quote: Unlabeled purified RNA targets were dephosphorylated using 1 U antarctic phosphatase (NEB) incubated with 1X of provided buffer during 20 min at 37°C and then 5 min at 65°C to inactivate the enzyme ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 nL BSA (20 ng/ml NEB) and 91.25 nL nuclease-free H2O ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.2 nL BSA 20 ng/ml (NEB), 23.3 nL 10X PNK buffer (NEB ...
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bioRxiv - Biophysics 2021Quote: ... and 39x parS were produced by PCR using a high-fidelity polymerase (Phusion Polymerase, NEB) (see Table S1 for primer sequences) ...
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bioRxiv - Microbiology 2020Quote: ... A DNase I RNAse free 2000 U/ml (NEB) treatment was done using 1 μl for 15 minutes at 37°C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 0.4 μl DNAse 1 (2000 U/ml) (New England Biolabs), and 10 μl RNAse A (10 mg/ml ...
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bioRxiv - Biophysics 2023Quote: ... the digoxigenin-labeled and unlabeled amplicons were ligated with T7 ligase (New England BioLabs) in a specific ratio of 1:2 ...
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bioRxiv - Immunology 2024Quote: ... 1 µL of 2000 U/mL DNase I (New England Biolabs) was added to bring the final reaction volume to 10 µL ...
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bioRxiv - Biochemistry 2021Quote: ... The alkyne-PAR was purified to remove excess alkyne-PEG1-amine using the Monarch Nucleic Acid Cleanup Kit (NEB) following the recommended protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Reactions were cooled to 4°C for 30 seconds and quenched with 1.2 mM unlabeled SAM (NEB) and blotted onto Hybond-XL membrane ...
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bioRxiv - Genomics 2022Quote: ... 1% bovine serum albumin (BSA) solution (NEB, 20 mg/mL). Nuclei were pelleted at 600 RCF for 5 minutes at 4C ...
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bioRxiv - Microbiology 2020Quote: RNA recovered from the PA-ac4C-seq and PAR-CLIP procedures were used for cDNA library preparation using the NEBNext Small RNA Library Prep Set for Illumina (NEB E7330S), then sequenced using Illumina NextSeq 500 ...
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bioRxiv - Neuroscience 2021Quote: ... Digestion solution containing proteinase K (8U/mL, New England BioLabs, P8107S) was applied to gels and allowed to digest for 2-16 hours (see Results ...
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bioRxiv - Genomics 2021Quote: ... 2 μL of 1 mg/ml bovine serum albumin solution (NEB), 1 μL of hOGG1 (ProSpec TechnoGene Ltd. ...
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bioRxiv - Evolutionary Biology 2020Quote: ... in the final concentration of 30 ng/ml and ATP (New England Biolabs) in the final concentration of 0.5 mM ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNA was PCR-amplified for 25 cycles with 5’-Cy3-labelled reverse primers (IDT) and unlabeled forward primers using either Taq polymerase or Phusion high-fidelity polymerase (NEB). PCR products were separated on 40cm tall 6% polyacrylamide denaturing gels and then visualized using a Molecular Dynamics Typhoon Scanner ...
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bioRxiv - Developmental Biology 2021Quote: DNA probes were generated by annealing 5’ IRDye®700 labeled forward oligonucleotides with unlabeled reverse oligonucleotides (Integrated DNA Technologies) to a final concentration of 5 μM in PNK buffer (New England Biolabs). One hundred femtomoles of labeled IRDye®700 probes were used in a 20-μl binding reaction containing 10 mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... and the radiolabeled 3’ fragment was ligated to an unlabeled 5’ fragment by splinted ligation with T4 DNA ligase (NEB) at 30°C for 2 h ...
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bioRxiv - Cell Biology 2021Quote: ... The solution was diluted by adding 7 ml of ligation buffer (NEB) containing 1% Triton X-100 and 30 Units of T4 DNA ligase (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... 5 units (2.5 μL) of RNase free DNAse I (NEW ENGLAND BioLabs, 2000 u. mL-1) and 100 μL of nuclease free water was added for every 10 μg of RNA ...
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bioRxiv - Bioengineering 2019Quote: ... Unlabeled tracrRNAs were synthesized by IDT or through in vitro transcription (HiScribe™ T7 Quick High Yield RNA Synthesis Kit, NEB) using DNA templates containing a T7 promoter binding sequence and full length tracrRNAs ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmid length DNA binding experiments were performed with unlabeled circular or linearized M13mp18 single (New England Biolabs, M13mp18 single-stranded DNA) or double-stranded DNA (New England Biolabs ...
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bioRxiv - Bioengineering 2022Quote: ... 1 μL of ds-oligo solution was ligated into 50 ng BsaI-digested pGRNA-sacB-ccdB using T4 DNA Quick Ligase (New England Biolabs).
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bioRxiv - Molecular Biology 2019Quote: ... XPG solution was mixed with 10 ml of amylose resin (New England BioLabs) pre-equilibrated in dialysis buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... nucleic acids were digested by addition of 10 µL of DNaseI (2000 units/mL, New England Biolabs) and CaCl2 to a final concentration of 10 mM ...
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bioRxiv - Immunology 2024Quote: ... and 2 µL of 2000 U/mL DNase I to the IVT reaction mixture (New England Biolabs). Template DNA digestion was carried out at 37 °C for 30 minutes in a thermocycler block prior to purification of the RNA using a Monarch RNA Cleanup Kit (New England Biolabs) ...
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bioRxiv - Developmental Biology 2023Quote: One-cell stage zebrafish embryos were injected with 1-2 nl of injection solution containing 300 ng/μl of Cas9 enzyme (NEB# M0646T) and 12.5 ng//μl of sgRNA ...
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bioRxiv - Cancer Biology 2023Quote: Fresh omentum and omental HGSOC tumor metastasis biopsy samples were cut into small pieces and dissociated in digestion solution (1 mg/mL collagenase/Dispase [Sigma cat. no. 10269638001], 1 unit/mL DNase I [NEB, cat ...
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bioRxiv - Genetics 2020Quote: ... Zebrafish embryos were injected at the 1-cell stage with a 1 nL solution of bbs2 gRNA (200 ng/μL) and Cas9 protein (10 μM; New England BioLabs, Beverly, MA). Mutagenesis was confirmed by High Resolution Melt Analysis (HRMA ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.0-3.0 μg of DNA was used for a ligation reaction in a volume of 1 mL using 1.0 μL of 2000 U/μL T4 DNA Ligase (NEB) for 3C experiments ...
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bioRxiv - Microbiology 2020Quote: ... 6 µg of DNA was used for MmeI digestion in 200 µL (6 µg gDNA, 6 µL MmeI (2000 U/mL, NEB), 0.5 µL 32 mM S-adenosylmethionine ...
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bioRxiv - Cancer Biology 2022Quote: ... The remaining nuclei were resuspended in the NSB buffer supplemented with 1 mM CaCl2 and 2000 gel units/ml of MNase (New England Biolabs) and incubated at 37°C for 10 min ...
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bioRxiv - Biophysics 2023Quote: ... and subsequently mixed with ∼0.2 ng of coilable ∼3.3 kbp dsRNA and 1 mg/ml bovine serum albumin (BSA, New England Biolabs). The ∼3.3 kbp length includes only the stem ...
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bioRxiv - Genomics 2019Quote: ... The 5 µL of each PCR cycle sample along with the 20 µL digested sample, and titration of the low molecular ladder (100 ng, 200 ng, 400 ng) (NEB) were run on a 2% Agarose gel ...
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bioRxiv - Cell Biology 2024Quote: ... with or without added PKA (50 ng or 500 ng for gel analysis or 50 ng for LC-MS/MS, New England Biolabs) were combined with protein kinase buffer (PK buffer ...
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bioRxiv - Molecular Biology 2021Quote: Genomic DNA (500 ng) with spiked-in lambda DNA (1.25 ng) (NEB) was sheared using an M220 ultrasound sonicator (Covaris ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were incubated in NGS (NGS; New England BioLabs, Hitchin, Hertfordshire, UK) diluted in 0.1 M PBS (pH 7.4 ...
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bioRxiv - Neuroscience 2022Quote: ... Gels were then placed in digestion solution containing proteinase K (8U/mL, New England BioLabs, P8107S) and digested for 4 hours at room temperature.
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bioRxiv - Microbiology 2022Quote: ... each chamber is filled for 30 min with a 1 mg/ml solution of Streptavidin (NEB) and then passivated with a blocking solution (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... Gels were then placed in digestion solution containing proteinase K (8U/mL, New England BioLabs, P8107S) and digested for 4 hours at room temperature.
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bioRxiv - Bioengineering 2020Quote: ... T4 DNA ligase (2000 units, NEB) was added and the mixture was incubated at room temperature overnight ...
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bioRxiv - Cell Biology 2023Quote: ... 100 ng/µL injection mix (70 ng/µL 1 kb DNA ladder (NEB), 30 ng/µL plasmid of interest ...
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bioRxiv - Cell Biology 2019Quote: ... stained with oligo-conjugated WGA at a concentration of 2-5 μg/mL in 1× HBSS with 2000× diluted murine RNase inhibitor (New England Biolabs, M0314L) at 37 °C for 20 min ...
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bioRxiv - Biochemistry 2020Quote: ... chromatin and chromatin bound fractions were released from magnetic beads using binding buffer containing 5 mM CaCl2 plus an excess (2000 units/ 20 mL reaction) of micrococcal nuclease (MNase; NEB, M0247S) Beads were incubated for 5 minutes at 37 °C with shaking (1250 rpm) ...
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bioRxiv - Genomics 2020Quote: ... 500 ng of intact gDNA was digested overnight with 2.5 μl of MspI (20,000 units/ml; NEB cat. R0106S) in the manufacturer’s supplied buffer (40 μl total volume ...