Labshake search
Citations for New England Biolabs :
401 - 450 of 1372 citations for IGFBP2 Human T7 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2023Quote: ... For his purpose pEKEx2 was first linearized using SacI (NEB, USA). The gene for mCherry was amplified by PCR using the primer pairs low_mCherry_fw and low_mCherry_rev (Table S1 ...
-
bioRxiv - Cell Biology 2023Quote: ... For all Gibson Assemblies the NEB Hi-Fi Assembly Mastermix (NEB) was used ...
-
bioRxiv - Biophysics 2021Quote: ... This vector was transformed into T7 express cells (enhanced BL21 derivative, NEB C2566I), grown to OD∼0.4-0.8 ...
-
bioRxiv - Molecular Biology 2021Quote: ... which was treated with 5 units of T7 endonuclease 1 (New England Biolabs) for 20 min at 37 °C and analyzed by 2% agarose gel electrophoresis ...
-
bioRxiv - Developmental Biology 2021Quote: ... dsRNA was produced by simultaneous transcription with T7 RNA polymerase (New England Biolabs) on templates containing T7 promoter sequences at both ends ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Constructs were transcribed using T7 polymerase and (10X) transcription buffer (New England Biolabs), RNAse inhibitor (Ribolock) ...
-
bioRxiv - Developmental Biology 2021Quote: ... The guide sequence was transcribed in vitro with NEB HiScribe T7 (NEB E2040S). The guide and TrueCut Cas9 v2 protein (ThermoFisher A36497 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 1 µl of T4 or T7 DNA Ligase (New England Biolabs, Ipswich, USA), 1 µl of BsaI-HFV2 (New England Biolabs) ...
-
bioRxiv - Cancer Biology 2019Quote: ... The hybridized DNA was digested with T7 endonuclease I (T7E1, New England Biolabs), and run on 2% agarose gel ...
-
bioRxiv - Genetics 2019Quote: ... and then treated with 5 units of T7 endonuclease 1 (New England Biolabs) for 30 min at 37°C and finally analyzed by 2% agarose gel electrophoresis.
-
bioRxiv - Genomics 2020Quote: ... and then held at 4°C until a subsequent T7 Endonuclease I (NEB) treatment at 37°C for 30 to 90 minutes ...
-
bioRxiv - Immunology 2021Quote: ... and then transcribed with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB) [23] ...
-
bioRxiv - Bioengineering 2021Quote: ... The template was synthesized using HiScribe t7 Quick High Yield RNA Synthesis (NEB) at 37 °C for 16 hrs ...
-
bioRxiv - Bioengineering 2020Quote: ... and polyadenylated using HiScribe ARCA T7 mRNA Kit (with tailing) (New England Biolabs) according to the manufacturer’s instructions.
-
bioRxiv - Biochemistry 2020Quote: ... a linear DNA template for T7 RNA polymerase was obtained by BstN1 (NEB) digestion of a plasmid carrying the corresponding gene ...
-
bioRxiv - Microbiology 2021Quote: ... PCR products were purified and in vitro transcribed using T7 HiScribe kit (NEB). Transcribed RNA products were run on a 5% acrylamide TBE-urea gel and visualized with 0.02% methylene blue in 1x TBE ...
-
bioRxiv - Neuroscience 2020Quote: ... and carried out in vitro transcription using the HiScribe T7 Kit (NEB, E2040S) by incubating at 37°C for 8 hours ...
-
bioRxiv - Microbiology 2021Quote: ... HiScribe™ T7 High Yield RNA Synthesis Kit (New England BioLabs, catalog# E2040S) was used to make the crRNA/gRNA with the above prepared DNA template according to manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2021Quote: ... riboprobes were prepared by invitro transcription using T3 and T7 RNA polymerases (NEB), respectively ...
-
bioRxiv - Bioengineering 2022Quote: ... coli SHuffle T7 Express lysY and the restriction enzymes were purchased from NEB Japan ...
-
bioRxiv - Cancer Biology 2022Quote: ... The plasmid was maintained in T7 Express lysY/Ig Competent E.coli cells (NEB). To prepare the transposase ...
-
bioRxiv - Molecular Biology 2020Quote: ... One μL of T7 endonuclease I (no. M0302S; New England Biolabs, Ipswich, MA) was added to the mix and incubated for 15 min at 37°C ...
-
bioRxiv - Neuroscience 2020Quote: ... gRNA transcription was performed with HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s protocol and gRNAs were purified via Phenol:Chloroform:Isoamyl alcohol (25:24:1 ...
-
bioRxiv - Bioengineering 2020Quote: ... IVT was performed overnight at 37°C using the HiScribe T7 kit (NEB) following the manufacturer’s instructions (N1-methyl-pseudouridine modified) ...
-
bioRxiv - Molecular Biology 2022Quote: ... or HiScribe™ T7 mRNA Kit with CleanCap® Reagent AG (NEB E2080S) as per the manufacturer’s instructions ...
-
bioRxiv - Genomics 2019Quote: ... A 100 µL IVT reaction is set using HiScribe T7 polymerase (NEB #E2040S). The reaction contained ...
-
bioRxiv - Molecular Biology 2020Quote: HiScribe™ T7 Quick High Yield RNA Synthesis Kit (NEB, Cat. No. E2050S) was used for in vitro transcription of COVID19 N RNA according to manufacturer’s instructions.
-
bioRxiv - Synthetic Biology 2019Quote: NEB HiScribe T7 Quick High Yield RNA Synthesis Kit (#E2050S, New England Biolabs) was used for data shown in Figure 1 according to the manufacturers protocol with the addition of 2.25 mM DFHBI-1T (Tocris #5610 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... The final concentration of T7 RNA polymerase (New England BioLabs, Ipswich, MA, USA) was 0.8 U/μL ...
-
bioRxiv - Synthetic Biology 2020Quote: ... Insert (library) and vector (pLibAcceptorV2) were ligated using T7 DNA Ligase (NEB #M0318S), incubating at room temperature for 1 hour ...
-
bioRxiv - Developmental Biology 2021Quote: ... PCR products were converted to RNA using the T7 RNA Transcription Kit (NEB) and purified with RNEasy Kit (Qiagen ...
-
bioRxiv - Molecular Biology 2019Quote: A 100 μL IVT reaction is set using HiScribe T7 polymerase (NEB #E2040S). The reaction contained ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... 30 U/µL of T7 DNA Ligase (New England Biolabs, Product No.: M0318L), at room temperature for 25 min with agitation at 600 rpm ...
-
bioRxiv - Genetics 2020Quote: ... The phosphorylated double-stranded target sequences were ligated using T7 ligase (NEB #M0318) into the BbsI digested pSPgRNA plasmid and were transformed into DH5a competent cells (ThermoFisher #18265017) ...
-
bioRxiv - Genetics 2020Quote: ... PCP products were used as template for T7 endonuclease assay (New England BioLabs), following manufacturer instructions ...
-
bioRxiv - Immunology 2021Quote: ... mRNA coding for RBD was synthesized per manufacturer recommendation using Hiscribe T7 (NEB) with co-transcriptionnal CleanCap AG (Trilink) ...
-
bioRxiv - Microbiology 2021Quote: ... and RNA transcribed using a HiScribe T7 High Yield RNA Synthesis kit (NEB) following the manufacturers’ protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... pHia5ET was transformed into T7 Express lysY/Iq Escherichia coli cells (NEB C3013I). Overnight cultures were added to two 1 L cultures of LB medium supplemented with 50 µg/mL kanamycin and grown with shaking at 37°C to an OD600 of 0.8-1.0 ...
-
bioRxiv - Developmental Biology 2022Quote: ... gRNA transcription was performed with HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s protocol and gRNAs were purified via Phenol:Chloroform:Isoamyl alcohol (25:24:1 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 10,000 units Type IIs restriction enzymes (T7 DNA ligase, Esp31/BsaI-v2, NEB), and 1 μL T4 DNA ligase (NEB ...
-
bioRxiv - Developmental Biology 2022Quote: ... and LvWnt5a were transcribed using SP6 or T7 RNA polymerases (New England BioLabs) and labeled with digoxigenin (Roche) ...
-
bioRxiv - Biophysics 2023Quote: From the H6-RapA expression plasmid and pSNAP-tag(T7) plasmid (NEB #101137), we constructed a plasmid pKI1 (Addgene #199118 ...
-
bioRxiv - Molecular Biology 2022Quote: ... derived from the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs). RNA was then capped with 3′-Desthiobiotin-GTP and polyA-tailed using vaccinia capping enzyme and E ...
-
bioRxiv - Molecular Biology 2023Quote: ... T7 express or NiCo21 competent cells (C2529 New England Biolabs Inc., Ipswich, MA) were transformed with each clone ...
-
bioRxiv - Cell Biology 2023Quote: ... and standard cut-and-paste cloning using T7 DNA ligase (New England Biolabs). NESRev ...
-
bioRxiv - Molecular Biology 2023Quote: ... dsRNA was generated by in vitro transcription using the HiScribe T7 kit (NEB), according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... The final sgRNAs were in vitro transcribed using T7 RNA polymerase (NEB, E2050) and stored at -80 °C.
-
bioRxiv - Genetics 2023Quote: ... and mRNA was transcribed using the HiScribe T7 high yield RNA kit (NEB) using N1-methylpseudouridine (Trilink ...
-
bioRxiv - Molecular Biology 2023Quote: ... In vitro transcription was performed using a T7 Quick HiScribe kit (NEB, E2050S) according to manufacturer’s instructions with overnight incubation at 37°C ...
-
bioRxiv - Microbiology 2023Quote: ... 0.1 μL of T7 RNA Polymerase (High Concentration, New England Biolabs, Beverly, MA), 20 mM Tris-acetate pH 8.5 and DEPC-treated water ...