Labshake search
Citations for New England Biolabs :
501 - 550 of 1372 citations for IGFBP2 Human T7 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2022Quote: ... and IVT reactions were set up according to the HiScribe™ T7 (E2040S, NEB) or Sp6 (E2070S ...
-
bioRxiv - Genomics 2022Quote: ... we spike in 2.5 µL of 3M U/mL T7 DNA ligase (NEB M0318L) and continue incubating for 1 hour at 25C ...
-
bioRxiv - Molecular Biology 2022Quote: ... high yield in vitro transcription (HiScribe T7 Quick High Yield RNA Synthesis Kit, NEB), and cDNA synthesis reactions (Maxima H Minus Reverse Transcriptase ...
-
bioRxiv - Microbiology 2023Quote: ... The digoxigenin-labeled RNA probes were transcribed from PCR products containing a T7 RNA polymerase promoter fused to gapA sequences with T7 RNA polymerase (New England Biolabs) and DigRNA labeling mix (Sigma-Aldrich ...
-
bioRxiv - Genetics 2023Quote: ... followed by in vitro transcription with the T7 High Yield RNA Synthesis kit (NEB). Newly synthesized sgRNAs were purified using the MEGAclear kit (Life Technologies) ...
-
bioRxiv - Molecular Biology 2022Quote: ... The construct was transformed into sHuffle T7 Express cells47 (New England Biolabs, cat. C3029J) for protein expression ...
-
bioRxiv - Cell Biology 2022Quote: ... coli PURExpress system were generated from the T7 PURExpress plasmid (New England Biolabs, USA). pSpCas9(BB)-2A-Puro (PX459 ...
-
bioRxiv - Biochemistry 2023Quote: ... containing a His6-tagged Ulp1 gene was transformed into T7 Express competent cells (NEB) and grown in LB medium supplemented with ampicillin (100 μg/mL ...
-
bioRxiv - Genomics 2023Quote: ... sgRNA was synthesized using NEB’s HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB). For generation of transgenic fish ...
-
bioRxiv - Immunology 2023Quote: ... the Gemini 1.0 plasmid underwent in vitro transcription using T7 RNA polymerase (NEB, M0251L), followed by in vitro 5’ capping and 3’ polyadenylation ...
-
DeCOIL: Optimization of Degenerate Codon Libraries for Machine Learning-Assisted Protein EngineeringbioRxiv - Bioengineering 2023Quote: The Gibson products are transformed into T7 express competent Escherichia coli cells (NEB C2566). Individual colonies are grown in 600 μL Luria Broth (RPI L24400 ...
-
bioRxiv - Biochemistry 2023Quote: ... were transformed with Lpg2248 (LotA) plasmids and T7 express Escherichia coli competent cells (NEB) were transformed with Lpg1621 (LotB ...
-
bioRxiv - Biophysics 2023Quote: ... the digoxigenin-labeled and unlabeled amplicons were ligated with T7 ligase (New England BioLabs) in a specific ratio of 1:2 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... were transcribed and purified using HiScribe T7 High Yield Transcription Kit (New England BioLabs) and illustra Microspin G-50 Columns (GE Healthcare Life Sciences) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Rehybridized products were incubated with 1.5 μl T7 endonuclease I enzyme (New England Biolabs) at 37°C for 1 h and separated on 1.5% agarose gel.
-
bioRxiv - Genetics 2024Quote: ... followed by in vitro transcription with the T7 High Yield RNA Synthesis kit (NEB). Newly synthesized sgRNAs were purified using the MEGAclear kit (Life Technologies) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and transcribed using the HiScribe T7 High Yield RNA Synthesis Kit (NEB, Cat. # E2040S), template concentration 10 nM ...
-
bioRxiv - Developmental Biology 2024Quote: ... The gRNAs were synthesized using the HiScribe T7 High Yield RNA Synthesis Kit (NEB) and purified using the Monarch RNA Cleanup Kit (NEB ...
-
bioRxiv - Biochemistry 2024Quote: ... and synthesized using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, E2040S), following the manufacturer’s instructions ...
-
bioRxiv - Genomics 2021Quote: ... 25 μL NEBNext Hi-Fi 2x PCR Master Mix (NEB (Ipswich, MA, USA) M0541S) ...
-
bioRxiv - Microbiology 2020Quote: ... and ligation with T4 ligase (Bioconcept) or by Hi-Fi Gibson assembly (NEB). PCR were performed using Phusion polymerase (Life Technologies ...
-
bioRxiv - Cell Biology 2021Quote: His-SpCas9-GFP was expressed in and purified from BL21 (DE3, NEB, C2527H) bacteria as previously described 47 ...
-
bioRxiv - Molecular Biology 2023Quote: Hi-C libraries were treated with 3 U of T4 DNA polymerase (NEB) in the presence of 0.1 mM dGTP (NEB ...
-
bioRxiv - Microbiology 2024Quote: ... His- tagged proteins were purified by NEBExpress® Ni-NTA Magnetic Beads (NEB) according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2024Quote: ... and joined into the linearized vector by Hi-Fi Gibson DNA Assembly (NEB) according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2021Quote: ... sgRNA was in vitro transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). Samples were incubated at 37°C for 16 hours before purifying the sgRNA with Invitrogen TRIzol Reagent (Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2021Quote: ... sgRNAs or crRNAs were transcribed using the HiScribe T7 High Yield RNA Synthesis kit (NEB) or the MEGAscript T7 Transcription kit (Thermo Fisher) ...
-
bioRxiv - Genetics 2021Quote: ... by PCR amplification of target locus followed by T7 Endonuclease I assay (New England Biolabs).
-
bioRxiv - Developmental Biology 2021Quote: ... The capped mRNAs were in vitro synthesized with the HiScribe T7 ARCA mRNA Kit (NEB) and purified with an RNA Clean & Concentrator-25 kit (Zymo Research ...
-
bioRxiv - Developmental Biology 2021Quote: mRNA was synthesized using the HiScribe™ T7 ARCA mRNA Kit (with tailing) (NEB, E2060S) and a PCR product amplified from the Kaede-NLS plasmid (addgene ...
-
bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin (DIG)-labeled RNA probes were synthesized using T7 or T3 RNA polymerases (NEB, USA) with the DIG RNA Labeling Mix (Roche ...
-
bioRxiv - Molecular Biology 2020Quote: ... and protein purity and concentration were analyzed along with T7 RNAP (New England Biolabs, #M0251S) by 10% SDS-PAGE gel electrophoresis stained with Coomassie blue (Bio-rad).
-
bioRxiv - Genomics 2020Quote: ... two templates with T7 promoter sequences were generated by performing PCR amplification (Q5 Polymerase, NEB) off of GeCKO v2.0 plasmid DNA using H1T7 and H1R primers for probe 1 and primers T2RT7 and T2F for probe 2 (Supplemental Table 4) ...
-
bioRxiv - Biochemistry 2019Quote: ... RNA sequences were transcribed in vitro as previously described42 using T7 RNA Polymerase (NEB #M0251). Chemically synthesized DNA oligonucleotides or transcribed RNA species were separated on a TBE-urea polyacrylamide gel ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... We first produced the DNA-template for T7-transcription using PCR with Taq polymerase (NEB) and primers 11 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... coli strain DH5α was used for plasmid construction while SHuffle T7 Express (New England Biolabs) 20 was used for cyclonal expression and library selections ...
-
bioRxiv - Bioengineering 2020Quote: RNA were transcribed using the HiScribe T7 high yield RNA synthesis kit (New England Biolabs) according to manufacturer’s instructions ...
-
bioRxiv - Biophysics 2020Quote: ... in-vitro transcription using the commercial HiScribe™ T7 High Yield RNA Synthesis Kit (NEB) gave rise to two complementary ssRNAs without any non-complementary nucleotides at their ends ...
-
bioRxiv - Microbiology 2021Quote: Recombinant proteins were expressed in Escherichia coli T7 Express lysY/Iq cells (New England Biolabs). For all proteins except PP1γ ...
-
bioRxiv - Biochemistry 2022Quote: RNA synthesis was performed using the HiScribe T7 High Yield RNA synthesis kit (NEB #E2040S) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2022Quote: ... IVT was performed to generate dsRNA using the T7 High Yield RNA Synthesis Kit (NEB). Template DNA was removed using Turbo DNAse (Ambion ...
-
bioRxiv - Cell Biology 2021Quote: ... Obtained cDNA was linearly amplified by overnight IVT (HighScribe T7 High Yield RNA synthesis, NEB) at 37°C under T7 promoter ...
-
bioRxiv - Plant Biology 2021Quote: ... dsRNA was produced using the HiScribe T7 High yield RNA synthesis kit (New England BioLabs). Templates were PCR products with T7 promoter sequences added to both ends to facilitate bidirectional transcription using the oligonucleotides listed in Table S1 ...
-
bioRxiv - Molecular Biology 2020Quote: ... The 0A_GQS and 2A_GQS designed RNAs were transcribed in vitro with T7 polymerase (NEB, 2040S) as substrates ...
-
bioRxiv - Bioengineering 2021Quote: ... 0.0625 µL T7 DNA ligase (Enzymatics),0.0625µL of 20 mg/mL Bovine Serum Albumin (NEB), 2x Reaction Ligation Buffer (Enzymatics ...
-
bioRxiv - Genetics 2022Quote: ... RNA was generated using the Hiscribe™ T7 Quick High Yield RNA Synthesis Kit (NEB) followed by purification using the RNeasy MinElute Cleanup Kit (Qiagen).
-
bioRxiv - Systems Biology 2022Quote: ... In vitro transcription (IVT) was performed with the HiScribe™ T7 RNA Synthesis Kit (NEB). For probes in the original protocol ...
-
bioRxiv - Immunology 2022Quote: ... The in vitro transcription was done using HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2019Quote: ... in vitro transcription using T7 High Yield RNA Synthesis Kit (Cat# E2040, New England Biolabs) and purification with RNeasy Plus Mini Kit (Qiagen ...
-
bioRxiv - Biochemistry 2019Quote: ... The sgRNAs were prepared by an in vitro transcription using T7 RNA polymerase (M0251S, NEB). The sgRNA transcription template was prepared by PCR amplification of sgRNA-coding sequence cloned in the sgRNA expression plasmid (pEASY-csgRNA ...