Labshake search
Citations for New England Biolabs :
551 - 600 of 1372 citations for IGFBP2 Human T7 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... The second strand was synthesized with 4 U of T7 DNA polymerase (New England BioLabs) and purified with HighPrep PCR beads (MagBio) ...
-
bioRxiv - Genetics 2020Quote: ... sgRNA synthesis was performed using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB), and purified using the RNA Clean & Concentrator-25 kit (Zymo Research ...
-
bioRxiv - Microbiology 2021Quote: ... The DNA was in vitro transcribed by using the HiScribe T7 ARCA mRNA kit (NEB) and capped RNA was isolated by using an RNA Clean & Concentrator (Zymo Research ...
-
bioRxiv - Synthetic Biology 2020Quote: ... pLib vector was then ligated with the GFP insert using T7 DNA Ligase (NEB #M0318S), incubating at room temperature for 1 hour ...
-
bioRxiv - Developmental Biology 2019Quote: ... Mutations were identified by amplicon restriction using restriction enzymes or T7 endonuclease I (#M0302L NEB) and verified by sequencing ...
-
bioRxiv - Cell Biology 2020Quote: ... using the HiScribe T7 ARCA mRNA kit with poly-A tailing (NEB, Ipswich, MA, USA). A combination of 3 separate reactions was used to achieve high concentrations (≥ 45 μg ...
-
bioRxiv - Biochemistry 2021Quote: ... we first used the HiScribeTM T7 Quick High Yield RNA Synthesis Kit (NEB, No. E2050S) to in vitro transcribe the pBESTlucT7 plasmid and generate pBESTlucT7 mRNA using the protocol provided in the manufacturer’s instruction manual [27] ...
-
bioRxiv - Molecular Biology 2021Quote: RNA constructs were made by in vitro transcription with T7 RNA polymerase (New England Biolabs) as previously described50 ...
-
bioRxiv - Immunology 2020Quote: ... then transcribed in vitro using HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, #E2040S). sgRNAs were purified by RNA Clean & Concentration™ (Zymo Research ...
-
bioRxiv - Developmental Biology 2021Quote: ... sgRNA was then transcribed from this template using HiScribe T7 in-vitro transcription kit (NEB) and ...
-
bioRxiv - Cell Biology 2021Quote: ... PCR products were used directly as templates for IVT using HiScribe T7 transcription kit (NEB). Resulting RNA was purified using RNAClean XP beads (Beckman Coulter ...
-
bioRxiv - Genomics 2021Quote: ... Debranching was performed by incubation with 15 units of T7 endonuclease I (New England Biolabs) for 15 minutes at 37 °C ...
-
bioRxiv - Cancer Biology 2022Quote: ... we PCR amplified a mCMV from a plasmid using primers XhoI_attL1_mCMV_FW and SalI_mCMV_RV followed by T7 ligation (NEB) of both PCR products ...
-
bioRxiv - Microbiology 2022Quote: ... 0.1 μM DNA annealed oligos and 1 unit/μL T7 RNA polymerase (New England Biolabs). Reactions were incubated at 37°C for 5 hours ...
-
bioRxiv - Microbiology 2022Quote: ... the PCR product was transcribed using HiScribe T7 High Yield RNA Synthesis Kit (E2040S; NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... sgRNAs were synthesized by in vitro transcription (T7 High Yield RNA Synthesis Kit, NEB, E2040S). Cas9 mRNA (100 ng/uL ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The barcode plasmid libraries were used to transform T7 Express chemically competent cells (NEB #C2566I) to establish barcoded E ...
-
bioRxiv - Molecular Biology 2022Quote: ... Synthesis was performed with the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs), according to the manufacturer’s instructions ...
-
Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv - Molecular Biology 2023Quote: ... RNAs were in vitro transcribed with HiScribe T7 ARCA mRNA Kit with tailing (NEB, E2060S) following the manufacturer’s recommended protocol ...
-
bioRxiv - Biochemistry 2023Quote: ... or HiScribe® T7 High Yield RNA Synthesis Kit (New England BioLabs, cat. no. E2040S) following the manufacturers’ protocols ...
-
bioRxiv - Molecular Biology 2023Quote: ... or uncapped U1 snRNAs were produced by in vitro transcription using T7 RNA polymerase (NEB). Briefly ...
-
bioRxiv - Synthetic Biology 2023Quote: ... IVT mRNA was produced using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040) along with 500 ng of linear template and 4 mM CleanCap AG Reagent (Trilink ...
-
bioRxiv - Molecular Biology 2023Quote: ... RNA substrates were synthesized by run off in vitro transcription (IVT) using T7 polymerase (NEB) in the presence of [α-32P]-UTP according to the manufacture’s protocol ...
-
bioRxiv - Synthetic Biology 2023Quote: All ligases (T3, T4, E.coli, T7, Taq) used during this work were obtained from NEB. Ligations were performed as indicated by the supplier ...
-
bioRxiv - Biochemistry 2023Quote: ... NC-DBD and DBD 2C-DBD were ligated into pET28-a using T7 ligase (NEB) and subsequently transformed into chemically competent E ...
-
bioRxiv - Immunology 2023Quote: ... The resulting double-stranded DNA was treated with T7 exonuclease (New England Biolabs, Ipswich, MA) to selectively degrade the unmodified strand of the dsDNA molecule ...
-
bioRxiv - Immunology 2023Quote: ... and saRNA was transcribed in vitro using HiScribe T7 High Yield RNA Synthesis Kit (NEB). After DNase treatment ...
-
bioRxiv - Biophysics 2023Quote: ... PCR was used to amplify templates for in vitro transcription with T7 RNA polymerase (NEB). The RNA product was extracted with phenol and concentration was measured by Nanodrop spectrophotometer (Thermo Fisher) ...
-
bioRxiv - Microbiology 2023Quote: ... RNAs were transcribed using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs). Reactions were set up as per manufacturer’s instructions with the addition of 4 units of Murine RNase Inhibitor (NEB ...
-
bioRxiv - Biophysics 2024Quote: ... The purified dsDNA templates were transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... mRNA was transcribed with HiScribe T7 mRNA Kit with CleanCap Reagent AG (New England Biolabs) according to manufacturer’s protocol with 1 µg of amplified template ...
-
bioRxiv - Developmental Biology 2023Quote: ... mRNA was synthesized from linearized plasmid using HiScribe T7 high yield RNA synthesis kit (NEB), then capped with m7G(5’)ppp(5’)G (NEB ...
-
bioRxiv - Molecular Biology 2024Quote: ... Robust synthesis of expected RNA product was comparable to commercially available T7 RNA polymerase (NEB).
-
bioRxiv - Genomics 2024Quote: ... For this HiScribe® T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswitch, MA) was used according to the manufacturer’s instructions ...
-
bioRxiv - Genetics 2024Quote: The puromycin targeting sgRNA was synthesized by in vitro transcription using T7 RNA polymerase (NEB) and template oligos ...
-
bioRxiv - Developmental Biology 2022Quote: ... The complete insert region was PCR amplified using Q5 Hi Fidelity DNA polymerase (NEB) and the amplicon sequenced at high coverage using Illumina MiSeq in the Complete Amplicon Next-Generation Sequencing service from the MGH CCIB DNA Core (Fig ...
-
bioRxiv - Microbiology 2021Quote: ... Reactions were conducted using NEB Phusion Hi Fi Polymerase (New England Biolabs, Ipswich, MA). Reactions were comprised of 4 µl 5X Phusion Buffer ...
-
bioRxiv - Microbiology 2021Quote: ... Reactions were conducted using NEB Phusion Hi Fi Polymerase (New England Biolabs, Ipswich, MA). Reactions were comprised of 4 µl 5X Phusion Buffer ...
-
bioRxiv - Cancer Biology 2021Quote: ... 40 μg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20°C to remove of biotin from non-ligated fragment ends ...
-
bioRxiv - Biochemistry 2020Quote: ... His-SNAP-EB3 proteins were then coupled to SNAP-Cell 647-SiR dye (NEB) by incubation at 37°C for 30 minutes ...
-
bioRxiv - Microbiology 2021Quote: ... The expression vector pET302/NT-His was digested with EcoR1 restriction enzyme (NEB R0101S) and run on a 1.5 % agarose gel ...
-
bioRxiv - Molecular Biology 2019Quote: ... 40 µg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20°C ...
-
bioRxiv - Synthetic Biology 2019Quote: ... cloning was performed with the NEBuilder Hi Fi DNA Assembly kit (New England Biolabs). PCR reactions were performed with the Phusion High-Fidelity PCR Master Mix with HF buffer (New England Biolabs) ...
-
bioRxiv - Genetics 2019Quote: ... These were used in a PCR reaction with Q5 Hi-Fidelity polymerase (NEB, USA) in combination with the pLuc-GAL5.1 reporter construct previously described 8 as template to produce pLuc-GALΔEGR ...
-
bioRxiv - Genomics 2019Quote: ... 40 μg of Hi-C library DNA were incubated with T4 DNA polymerase (NEB) for 4 hours at 20°C ...
-
bioRxiv - Cancer Biology 2021Quote: ... using the NEBuilder Hi Fi DNA Assembly Master Mix (New England Biolabs, Beverly, MA). For the production of lentiviral particles ...
-
bioRxiv - Biochemistry 2023Quote: ... The His-MBP-Cas12a plasmid was transformed into BL21(DE3) cells (New England Biolabs). A single colony was used to inoculate LB media supplemented with 50μg/ml Kanamycin for an overnight culture grown at 37°C ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... stained in HI buffer containing 5 μM SNAP-Surface Alexa 647 (New England Biolabs) for 15 minutes at room temperature ...
-
bioRxiv - Biochemistry 2023Quote: ... and K723G).49 His/MBP-BRAF NTs were created with standard Gibson Assembly (NEB) procedure in the pET28-MBP vector from the following primers ...
-
bioRxiv - Biophysics 2021Quote: ... the SNAP-tag fragment was digested from the pSNAP-tag (T7)-2 vector (New England Biolabs), inserted into pET-28b plasmid resulting in a construct referred as pET-SNAP ...