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Citations for New England Biolabs :
201 - 250 of 1372 citations for IGFBP2 Human T7 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... WGA samples were treated with T7 endonuclease I (NEB) to maximize nanopore sequencing yield according to ONT documentation ...
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bioRxiv - Microbiology 2020Quote: ... A HiScribe T7 High Yield RNA Synthesis Kit (NEB) was used for RNA synthesis ...
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bioRxiv - Microbiology 2019Quote: ... followed by ligation with T7 ligase (New England Biolabs) for 30 mins at 25°C ...
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bioRxiv - Genomics 2022Quote: ... The RCA product was debranched with T7 endonuclease (NEB) for 2 hours at 37C then cleaned using a Zymo DNA Clean & Concentrator column-5 (Zymo #D4013) ...
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bioRxiv - Synthetic Biology 2022Quote: ... The plasmid was transformed into T7 express cells (NEB) according to manufacturer’s instructions and expressed in Luria-Bertani broth at 37 °C with shaking at 180 rpm before induction by IPTG at an optical density of 0.6 ...
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bioRxiv - Genetics 2022Quote: ... 2.5 μl T7 RNA polymerase (50 U/μl; NEB) at 37 °C for 2 hours ...
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bioRxiv - Microbiology 2022Quote: ... 0.5μl [α-32P]rUTP and 1μl T7 polymerase (NEB). After incubation at room temperature for 2 hours the NS5B RdRp reactions were placed on ice while the T7 reactions were supplemented with 1 unit RNase-free DNase and incubated at 37°C for 20 minutes ...
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bioRxiv - Microbiology 2022Quote: ... HiScribe™ T7 High Yield RNA synthesis kit (NEB) was used as per the manufacturer’s protocol with Biotin Labelling RNA Mix (Roche ...
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bioRxiv - Biochemistry 2020Quote: ... coli strain T7 Express (New England Biolabs, Ipswich, USA) at 37 °C in 5 mL LB medium containing 25 μg mL−1 kanamycin by induction with 1 mM IPTG for 3 hours ...
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bioRxiv - Biochemistry 2020Quote: ... coli strain T7 Express (New England Biolabs, Ipswich, USA) as described in section “Proteolytic inhibition assays” ...
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bioRxiv - Developmental Biology 2022Quote: ... and transcribed with the Maxi script T7 kit (NEB), following manufacturers’ instructions ...
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bioRxiv - Genomics 2022Quote: ... and 8 units/μL T7 RNA Polymerase (NEB, M0251S) in the transcription buffer (40 mM Tris-HCl pH 8 ...
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bioRxiv - Molecular Biology 2022Quote: ... and in vitro transcribed using T7 RNA polymerase (NEB). F ...
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bioRxiv - Cell Biology 2019Quote: ... 4 U/μl T7 RNA polymerase (NEB - pH 7.9) in a total volume of 150 µl ...
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bioRxiv - Immunology 2020Quote: ... Editing efficiency was estimated by T7 endonuclease I (NEB) assay according to the manufacturer’s instructions using the following primers ...
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bioRxiv - Biochemistry 2020Quote: ... E.coli T7 Express strain (New England BioLabs, Cat#C2566) was used for overexpression of LacI and M.HpaII ...
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bioRxiv - Microbiology 2020Quote: ... The HiScribe T7 High Yield RNA Synthesis Kit (NEB) was used to generate dsRNA from the purified T7-tagged amplicons ...
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bioRxiv - Cancer Biology 2019Quote: ... and subjected to T7 endonuclease I (New England Biolabs) digestion for 30 minutes at 37°C ...
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bioRxiv - Neuroscience 2019Quote: ... T7 Endonuclease I assay (M0302, New England Biolabs Inc.) and Sanger sequencing (GENEWIZ ...
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bioRxiv - Biochemistry 2020Quote: ... coli strain T7 Express (New England Biolabs, Ipswich, USA) in 2 L 25 μg mL−1 kanamycin-containing LB medium by induction with 1 mM isopropyl-β-D-thiogalactoside (IPTG) ...
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bioRxiv - Cell Biology 2020Quote: ... and 30 units of T7 DNA polymerase (NEB, M0274) to the annealed oligonucleotide–ssDNA mixture ...
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bioRxiv - Neuroscience 2020Quote: ... we performed a T7 endonuclease I (T7EI) assay (NEB) on approximately 10 F1 embryos from a clutch to confirm whether any mutations had occurred in the germline of the injected parent ...
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bioRxiv - Immunology 2022Quote: ... T7 RNA polymerase (New England BioLabs cat. no. M0251S), Yeast Inorganic Pyrophosphatase (New England BioLabs cat ...
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bioRxiv - Molecular Biology 2022Quote: ... was transformed into T7 Express LysY/Iq (NEB C3013). Two 1 L of LB supplemented with 100 µg/mL ampicillin were inoculated with 10 mL of overnight culture ...
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bioRxiv - Biochemistry 2022Quote: ... coli SHuffle T7 Express (New England Biolabs, Ipswich, MA), which is used to express disulfide bond-containing proteins such as antibodies (Jeong et al. ...
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bioRxiv - Biochemistry 2022Quote: ... 1× reaction buffer for T7 RNAP (New England BioLabs) and 125 U of T7 RNAP (New England BioLabs) ...
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bioRxiv - Biochemistry 2022Quote: ... and 125 U of T7 RNAP (New England BioLabs). For capped RNA ...
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bioRxiv - Genomics 2022Quote: ... 4 U of T7 DNA polymerase (New England Biolabs) were used to perform second-strand synthesis and DNA was purified using CleanPCR beads ...
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bioRxiv - Molecular Biology 2022Quote: ... The amplicon was transcribed with HiScribe T7 Quick (NEB) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: ... supplemented with 0.2 U/μL of T7 Exonuclease (NEB) and incubated at 25 °C overnight before being heated to 95 °C and slowly annealed (−0.1 °C/sec ...
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bioRxiv - Cell Biology 2022Quote: ... vector using T7 ligase (New England Biolabs, cat# M0318S) according to the manufacturer’s protocol.
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bioRxiv - Synthetic Biology 2022Quote: ... 5 U/μl T7 RNA polymerase (New England Biolabs), 1 U/μl SUPERase In (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2022Quote: ... and 5 U/µl T7 RNA polymerase (NEB M0251) in 1x RNAPol buffer (NEB) ...
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bioRxiv - Developmental Biology 2022Quote: ... then annealed and assayed with T7 endonuclease (NEB M0302S) to look for mismatched nucleotide bases ...
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bioRxiv - Genetics 2023Quote: ... followed by T7 endonuclease I assay (New England Biolabs) assay to detect mutations ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Hiscribe T7 RNA synthesis kit (NEB #E2040S). In brief ...
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bioRxiv - Cancer Biology 2023Quote: ... T7 endonuclease I enzyme was purchased (New England BioLabs) and assay was performed followed by the manufacturer’s protocol.
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bioRxiv - Biophysics 2023Quote: ... The template was then transcribed with T7 polymerase (NEB), in transcription buffer (100 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... coli SHuffle T7 cells (New England Biolabs, Ipswich, MA) in TB medium at 17 °C ...
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bioRxiv - Cell Biology 2023Quote: ... T7 RNA polymerase and RNAPol Reaction Buffer (NEB,M0251) used in our reaction.
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bioRxiv - Developmental Biology 2023Quote: ... followed by T7 endonuclease I assay (New England Biolabs) to detect indel mutations ...
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bioRxiv - Neuroscience 2023Quote: ... were transcribed with SP6 or T7 RNA polymerase (NEB). After transcription ...
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bioRxiv - Developmental Biology 2023Quote: ... plus the appropriate RNA Polymerase T7 or Sp6 (NEB). Probes were purified with RNA Clean Up Kit (Qiagen ...
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bioRxiv - Genomics 2024Quote: ... The concatemers are further treated with T7 Endonuclease (NEB) to remove branches before being prepped for Nanopore sequencing.
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bioRxiv - Microbiology 2024Quote: ... and T7 DNA ligase (New England Biolabs Cat. #M0318S), then electroporated into Endura Duo electrocompetent cells (Biosearch Technologies Cat ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... in an all-in-one T7 ligase (NEB#M0318S) based Golden-Gate assembly (37° C for 5min ...
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bioRxiv - Neuroscience 2023Quote: ... used in T7 endonuclease I activity assays (NEB E3321) as previously described (Baker et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1 μL of T7 Ligase (New England Biolabs, M0318S), 0.4 μL of Fermentas FD Eco31I (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... coli Shuffle T7 Express cells (C3029J, New England Biolabs) to aid disulfide bond formation ...
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bioRxiv - Biophysics 2024Quote: ... coli SHuffle T7 Express (New England Biolabs, Ipswich, MA) cells were transformed with 100 ng pET32-Trx-LqhαIT and ...