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Citations for New England Biolabs :
551 - 600 of 2329 citations for Human GSDMC shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The LRRC8A-GFP-PhoCl-TMEM plasmid was digested with AgeI (NEB) and EcoRI (NEB ...
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bioRxiv - Neuroscience 2023Quote: Plasmid constructs were linearized using XbaI restriction enzyme (New England Biolabs) to create DNA templates for complementary RNA (cRNA ...
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bioRxiv - Systems Biology 2024Quote: Each tagged-ORF plasmid subpool was treated with I-SceI (NEB) in the provided buffer (at a volume of 50uL for ∼1ug hORF plasmids and 200uL for ∼4ug vORF plasmids) ...
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bioRxiv - Developmental Biology 2023Quote: ... Plasmid ISceI/MCS-d1GFP-SV40/ISceI was digested with XhoI (NEB) and SmaI (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... All plasmids were transformed into Escherichia coli Stable3 competent cells (NEB). The identity of all plasmids was confirmed by Sanger Sequencing ...
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bioRxiv - Neuroscience 2024Quote: ... To clone inserts into plasmids NEBuilder® HiFi DNA Assembly (NEB) was used.
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bioRxiv - Cell Biology 2024Quote: Plasmid cloning was carried out using NEBuilder HIFI DNA assembly (NEB). Primer sequences are given in Table 1 ...
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bioRxiv - Immunology 2024Quote: ... the plasmids were linearized by digestion with SpeI (New England BioLabs), followed by purification using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) ...
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bioRxiv - Genetics 2024Quote: ... 1 µg of plasmid was incubated with 8 U M.SssI (NEB) at 37°C for 4 h for in vitro DNA methylation ...
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bioRxiv - Genomics 2024Quote: ... Tet-puro-pLKO plasmid was digested with AgeI and EcoRI (NEB) restriction enzymes ...
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bioRxiv - Microbiology 2024Quote: ... plasmid was double digested with NdeI and SpeI restriction enzymes (NEB) followed by gel purification of the 4725 bp band using a Wizard spin column (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... linear plasmid backbone was generated by restriction digestion with SmaI (NEB). Plasmid backbone DNA was treated with DpnI (NEB ...
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bioRxiv - Microbiology 2024Quote: ... The plasmids were constructed using T4 DNA ligase (New England Biolabs) with a ratio of 3:1 of insert to backbone ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids and inserts were assembled using NEBuilder HiFi DNA Assembly (NEB), followed by incubation at 50°C for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were assembled using isothermal HiFi assembly (New England Biolabs (NEB), Ipswitch ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were assembled using isothermal HiFi assembly (New England Biolabs (NEB), Ipswitch ...
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bioRxiv - Developmental Biology 2024Quote: ... pXPR_050 plasmids were digested using the Bsmb1 restriction enzyme (NEB; R0580). The synthesized double-strand oligo pools and digested pXPR_050 plasmids were ligated using T7 ligase (NEB ...
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bioRxiv - Bioengineering 2024Quote: The glr-1p::GCaMP6s plasmid was made by LR recombination (NEB) of a destination vector containing the DNA sequences for GCaMP6s (Chen et al. ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmid was linearized using BAMHI-HF (New England Biolabs, USA) following manufacturer protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... Purified human gDNA was digested with the restriction enzyme HaeIII (NEB, Ipswich, Massachusetts) per the manufacturer’s instructions to yield an average of 347-bp DNA fragments22 ...
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bioRxiv - Genomics 2019Quote: ... mouse NIH/3T3 and human Jurkat DNA were from NEB (Ipswich, MA, USA). E14 genomic DNA was extracted with a DNeasy Blood and Tissue Kit (QIAGEN ...
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bioRxiv - Genetics 2020Quote: ... following rRNA depletion using a NEBNext rRNA Depletion Kit (Human/Mouse/Rat) (NEB).
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bioRxiv - Molecular Biology 2023Quote: ... Human FBP1 mutants were constructed by Q5 Site-Directed Mutagenesis Kit (NEB, E0554S) in the PCDH-V5-FBP1 vectors ...
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bioRxiv - Biophysics 2022Quote: Recombinant wild-type human histone H1.0 was used (H1; New England Biolabs M2501S). ProTαC and unlabeled ProTα were prepared as previously described (26) ...
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bioRxiv - Plant Biology 2020Quote: ... Unmodified plasmids were digested with methylation-sensitive enzyme DpnI (New England Biolabs). The mutated plasmids were transformed in E ...
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bioRxiv - Biophysics 2021Quote: ... and cloned into BsaI-restricted PGEM 601 plasmids using T4 ligase (NEB). Ligation product was transformed into E ...
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bioRxiv - Biophysics 2021Quote: ... and cloned into KpnI-BamHI-digested pFL38 plasmid by T4 ligase (NEB). The AAC2 null strain was transformed with pFL38-AAC2-flag ...
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bioRxiv - Molecular Biology 2019Quote: ... Unmethylated control plasmid was produced in dam− bacteria (New England BioLabs #C2925H) and methylation status was confirmed by DpnI and DpnII digestion ...
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bioRxiv - Genetics 2022Quote: ... The linearised plasmid was blunted using T4 DNA polymerase (New England Biolabs) and re-ligated.
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bioRxiv - Neuroscience 2021Quote: ... pX330 vector (addgene plasmid #42230) was digested with BbsI (NEW ENGLAND BIOLABS), and each sgRNA sequence was cloned into the sgRNA cloning site as described above ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified plasmids were linearized by overnight digestion (37°) with EcoRI-HF (NEB) and the products were then visualized on 1% agarose gel ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmid constructs were modified by site-directed mutagenesis (E0554; New England Biolabs) to create the desired mutations in klp-6 and cwp-4 ...
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bioRxiv - Molecular Biology 2021Quote: PHO4 was cloned into the 2micron plasmid pRS424 using Gibson assembly (NEB). pGAL-H3-3xHA-URA3 on a single copy plasmid is a gift from the Michel Strubin lab.
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bioRxiv - Molecular Biology 2020Quote: ... After elimination of original plasmid template by digestion with DpnI (NEB R0176), amplification products were transformed into E ...
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bioRxiv - Biophysics 2022Quote: ... The plasmid was simultaneously digested with restriction endonucleases BsaI and BstXI (NEB). The resulting 2.6-kb fragment was separated from the 1.4-kb fragment and uncut plasmid by agarose gel purification (Promega Gel Wizzard Kit) ...
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bioRxiv - Cell Biology 2022Quote: ... the LentiGuide-Hygro plasmid was digested using BsmBI-v2 enzyme (NEB #R0739), and the larger band (~9 kb ...
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bioRxiv - Molecular Biology 2021Quote: ... The resultant plasmids were digested with PacI and PmeI (New England Biolabs) and used to transfect L ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... and minipreps were performed using the Monarch Plasmid Miniprep Kit (NEB T1010L). NEB 5-alpha Competent E ...
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bioRxiv - Developmental Biology 2019Quote: ... and cloned into the control plasmid using restriction enzyme XhoI (NEB, #R146L). pCAGGS-Cre plasmid was generated as described (Kranz et al. ...
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bioRxiv - Microbiology 2019Quote: ... Plasmid prib(+)XpA was digested by NruI and SnaBI (New England Biolabs) and ligated to produce prib(+)XpA lacking the poliovirus capsid-encoding region from 1175 to 2956 (prib(+)XpAΔ-1175-2956).
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bioRxiv - Molecular Biology 2019Quote: 50 µg of the plasmid DNA were cut with HindIII-HF (NEB) in Cut Smart buffer with 100 U of enzyme for 1.5 hours at 37°C in 50 µl ...
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bioRxiv - Genomics 2019Quote: ... The 3XFlag-pA-Tn5-Fl plasmid was transformed into C3013 cells (NEB) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... dP75 plasmid with mutations were generated by Site-Directed Mutagenesis Kit (NEB). For yeast two-hybrid assays ...
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bioRxiv - Microbiology 2021Quote: ... The parental plasmid was digested for 4 h using Dpn1 endonuclease (NEB) at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... expression plasmids pETgshA and pETgshB were assembled using HiFi Assembly MasterMix (NEB), and transformed into chemically competent E ...
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bioRxiv - Genomics 2021Quote: ... and cloned into plasmid pT2 using Gibson Assembly Cloning Kit (NEB, E5510S). Obtained pT2_FF plasmids were used as templates for PCR-based barcoded library preparation (22).
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bioRxiv - Cell Biology 2021Quote: ... This first intermediate plasmid was linearized via EcoRV (New England Biolabs-NEB) digestion and 2 inserts were added to it by In-Fusion to generate the second intermediate plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... This first intermediate plasmid was linearized via EcoRV (New England Biolabs-NEB) digestion and 2 inserts were added to it by In-Fusion to generate the second intermediate plasmid ...
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bioRxiv - Microbiology 2020Quote: The plasmid pAAV S1-Fc was digested with New England Biolabs (NEB) Restriction Enzymes Pvu I-HF (Cat ...
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bioRxiv - Microbiology 2021Quote: ... Cas9 and dCas9 plasmids were linearized with the PacI restriction enzyme (NEB) to enable plasmid integration at the NEUT5L locus ...