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Citations for New England Biolabs :
701 - 750 of 2329 citations for Human GSDMC shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... The plasmids were linearized using BamHI-HF (New England Biolabs, cat no. R3136L), column-purified (Qiagen ...
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bioRxiv - Developmental Biology 2020Quote: ... Plasmids were linearized using either NcoI and SpeI restriction enzymes (NEB, Ipswich, MA). Riboprobes were synthesized from the plasmids by in vitro transcription using either T7 or Sp6 polymerase and a digoxigenin (DIG ...
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bioRxiv - Developmental Biology 2021Quote: ... the hsp70l-ephrinB2a-p2A-Venus or hsp70l-venus plasmid and I-SceI (NEB) were co-injected into the blastomeres at the one-cell stage of Tg(kdrl:DenNTR ...
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bioRxiv - Molecular Biology 2021Quote: ... A plasmid encoding Exonuclease V (RecBCD) (New England Biolabs, Inc., Ipswich, MA, USA) was used as a template to amplify recB gene as three overlapping PCR fragments B1 (3080bp) ...
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bioRxiv - Microbiology 2020Quote: ... the source plasmid (p-retronWT) was mutagenized through PCR using a kit (NEB; Q5-Site-Directed Mutagenesis Kit ...
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bioRxiv - Immunology 2021Quote: ... The parental caspase-1 plasmid (methylated) was digested using DpnI (NEB; Cat,# R0176S). Plasmids were transformed in E.coli DH5α competent cells (NEB ...
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bioRxiv - Molecular Biology 2021Quote: All mRNA generating plasmids were digested with PvuII-HF (NEB R[Δ4]151L) and ApaLI-HF (NEB R0507L ...
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bioRxiv - Molecular Biology 2021Quote: ... the minigene plasmid library was digested with EcoRI and HindIII (New England Biolabs) and run on an agarose gel ...
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Parallelized engineering of mutational models using piggyBac transposon delivery of CRISPR librariesbioRxiv - Bioengineering 2020Quote: ... the resulting plasmid library and gBlock were digested with Esp3I (New England BioLabs) and gel purified ...
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bioRxiv - Evolutionary Biology 2019Quote: ... CPS mutant plasmids were constructed using the Q5 Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Bioengineering 2019Quote: ... and GA-SNAP-fwd/GA-SNAP-rev (template plasmid pSNAPf, New England Biolabs).
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bioRxiv - Biochemistry 2019Quote: ... amplified and cloned into the pHis17 plasmid using Gibson Assembly (New England Biolabs), with a stop codon before the C-terminal hexahistidine tag on the plasmid ...
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bioRxiv - Biochemistry 2019Quote: The plasmid containing the construct was linearized with Not-I HF enzyme (NEB) at 37°C for 2 h ...
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bioRxiv - Genomics 2020Quote: The vector backbone (lentiGuide-Puro, Plasmid no. 52963) was digested with Esp3I (NEB) and treated with rSAP (NEB ...
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bioRxiv - Microbiology 2019Quote: ... DNA restriction nucleases were used to digest plasmids (Promega and New England Biolabs). Gibson assembly mixes were used according to manufacturer instructions to construct the plasmids used in this study (New England Biolabs) ...
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bioRxiv - Genetics 2019Quote: ... Correct plasmids were then digested using Dral (New England Biolabs, Ipswich, MA, USA). Linearized plasmid was used as a template for RNA synthesis using the MEGAshortscript T7 Transcription Kit following the manufacturer's instructions (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... Plasmid DNA was in vitro methylated using CpG Methyltransferase (M.SssI, New England Biolabs), with the efficiency of methylation assessed by digestion using HpaII and HhaI methylation-sensitive restriction enzymes (NEB) ...
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bioRxiv - Biochemistry 2021Quote: ... The parental plasmid molecules were inactivated by DpnI treatment (1 h, NEB, USA) and the crude reaction mixture was transformed to electrocompetent E ...
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bioRxiv - Developmental Biology 2021Quote: Plasmid pJM1 (Pceh-23_L::mKate2::rgl-1) was generated using Gibson Assembly (NEB) of PCR products ...
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bioRxiv - Bioengineering 2021Quote: ... PCR amplicons were generated from plasmid templates using Q5 Hot Start Polymerase (NEB) and pooled into 100 µl reactions for Solid Phase Reversible Immobilization (SPRI ...
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bioRxiv - Bioengineering 2021Quote: ... the plasmid solutions were incubated for Nb.Bsmi nicking enzyme (R0706S, New England Biolabs) for 60 minutes at 65°C in NEBuffer 3.1 ...
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bioRxiv - Biochemistry 2020Quote: ... The template plasmid molecules were inactivated by DpnI treatment (1 h, NEB, USA) and directly transformed to electrocompetent E ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmid template DNA in the reaction was digested with DpnI (New England Biolabs) for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... The plasmid pAC-crRNAred (29) was digested with BsaI-HF (New England Biolabs), dephosphorylated with FastAP (ThermoFisher ...
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bioRxiv - Microbiology 2021Quote: ... Expression plasmids were assembled using the Gibson assembly kit (New England Biolabs, U.S.A) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... LentiCRISPRv2 plasmid was cut using BSMB1 restriction enzyme (New England Biolabs; Cat#: R0580S), and T4 DNA ligase (NEB ...
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bioRxiv - Biochemistry 2021Quote: Plasmid propagation and subcloning was performed using Escherichia coli DH10β competent cells (NEB). The codon optimized GTL gBlock (Supporting Information Table 1 ...
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bioRxiv - Microbiology 2020Quote: ... were combined together with YTK-compatible part plasmids in BsaI (New England BioLabs) golden gate reactions to yield plasmid pUDI168 ...
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bioRxiv - Systems Biology 2020Quote: ... We next column purified the assembled plasmid using Monarch DNA cleanup columns (NEB) according to the manufacturer’s protocol then electroporated 2 μL of the column purified plasmid into Endura Electrocompetent E ...
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bioRxiv - Microbiology 2021Quote: ... Addgene plasmid # 46888] using NEBuilder HiFi DNA assembly cloning kit (New England Biolabs) according to manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: The pooled library of Pveg-sgRNA plasmids was linearized with KpnI-HF (NEB), and was incorporated into the ICE::amyE locus by transformation into strain MMH211 and selecting for resistance to chloramphenicol ...
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bioRxiv - Microbiology 2021Quote: ... and cloned as a pool into a plasmid using Gibson Assembly (NEB E26115) as performed by Sidik et al ...
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bioRxiv - Plant Biology 2021Quote: ... The digested plasmid and the hybridized oligonucleotides were ligated using T4 Ligase (NEB) and then transformed into Escherichia coli DH5α competent cells ...
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bioRxiv - Synthetic Biology 2021Quote: Entry and expression plasmids were grown in NEB-5α cells (New England Biolabs). Destination plasmids were grown in One Shot ccdB survival cells (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... through ligation of both plasmids after digestion with BamHI (NEB, reg. no. R313614). The pMD2.G plasmid (#554 ...
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bioRxiv - Genomics 2020Quote: ... Plasmid DNA was purified from liquid cultures using DNA miniprep kit (NEB #T1010).
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bioRxiv - Synthetic Biology 2022Quote: ... These plasmids were transformed into chemically competent BL21(DE3) cells (New England Biolabs) and single transformants selected for using ampicillin resistance.
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bioRxiv - Cell Biology 2022Quote: ... Cloning into the pGFP33 plasmid were performed using the Gibson assembly kit (NEB). ARF1 point mutations were generated by Quick-change Mutagenesis kit (NEB ...
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bioRxiv - Cancer Biology 2022Quote: ... The plasmid was maintained in T7 Express lysY/Ig Competent E.coli cells (NEB). To prepare the transposase ...
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bioRxiv - Molecular Biology 2022Quote: ... the pJFH-1T plasmid was amplified using Q5 high fidelity DNA polymerase (NEB) and the EcoRI- T7-JFH1-U4C-FW (5’- GGC CAG TGA ATT CTA ATA CGA CTC ACT ATA GAC CCG CCC C-3’ ...
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bioRxiv - Microbiology 2022Quote: ... pPVM-2A-144-poliovirus-GFP plasmid DNA was linearized with EcoRI (NEB R3101). Purified linear DNA was then used to in vitro transcribe poliovirus plus stranded RNA using MEGAscript™ T7 Transcription Kit (Thermo Fisher Scientific AM1334 ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid PHO5 AS gDNA-URA3 was then obtained by Gibson assembly reaction (NEB) using OFS_2886 and OFS_2887 to amplify pFS3892 backbone and OFS_2888 and OFS_3095 for gDNA cloning.
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bioRxiv - Biochemistry 2022Quote: ... and the plasmids coding for the remaining segments were linearized using BsmBI (NEB). Following linearization ...
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bioRxiv - Biochemistry 2022Quote: ... or 79.85 fmol supercoiled plasmid DNA (KAC1151 or MNW95) in ThermoPol buffer (NEB) with a final concentration of 10mM MgSO4 ...
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bioRxiv - Immunology 2020Quote: ... and assembled into the plasmid vector with NEB HiFi assembly (New England BioLabs). An expression plasmid expressing the same pre-fusion stabilized full length transmembrane protein used the pcDNA3.1 backbone and was directly synthesized and cloned into the vector by GeneArt (Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2020Quote: ... Unmethylated pUC19 was extracted using the Monarch Plasmid Miniprep Kit (NEB, Ipswich, MA). 10 μg of pUC19 was CpG methylated in vitro using 40 units of M.SssI (NEB ...
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bioRxiv - Microbiology 2021Quote: All bacterial two-hybrid plasmids were constructed using the Gibson assembly mix (NEB) or the Hifi DNA assembly mix (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: Linear transformation cassettes were cut out of circular plasmids using EcoRV-HF (NEB) and transformed into wild-type strain T222+ or RBCS1 and RBCS2 double-knockout strains Cal13.1B-[5,46] or T60 [26] ...
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bioRxiv - Neuroscience 2020Quote: This plasmid was generated using NEBuilder HiFi DNA Assembly (New England Biolabs #E5520S), using the following fragments generated by PCR from the indicated template with the indicated primers:
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bioRxiv - Cancer Biology 2021Quote: ... The plasmid was amplified in DH5a cells (NewEngland Biolabs, Ipswich, MA; Cat #C2987H), and purified using plasmid purification midi-kit (Qiagen ...