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Citations for New England Biolabs :
351 - 400 of 2329 citations for Human GSDMC shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Sequence-verified plasmids were transformed into BL21 (DE3) cells (NEB). Starter cultures (5 mL ...
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bioRxiv - Neuroscience 2021Quote: ... 5µg of lentiviral CRISPR plasmid was digested with BsmBI (NEB) for 90 min at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... pRZ-R3C plasmid was treated with EcoRI-HF (NEB, USA). RNA was expressed using T7 RNA polymerase (homemade ...
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bioRxiv - Biochemistry 2020Quote: ... the original plasmid was digested with DpnI (New England Biolabs) for 2 hours at 37 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... Background plasmid was digested using Dpn1 (New England Biolabs, R0176L), and the remaining PCR product was purified again using spin columns ...
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bioRxiv - Plant Biology 2021Quote: ... and expression plasmid carrying AHK5HK-RD gene into ER2566 (NEB) strain ...
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bioRxiv - Neuroscience 2022Quote: ... the template plasmid was linearized with Spe1 (New England Biolabs) and transcribed with T7 polymerase (Roche) ...
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bioRxiv - Genetics 2022Quote: ... We digested each TFT plasmid with SacI and BglII (NEB) to obtain a linear DNA transformation fragment (Supplementary Figure 10) ...
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bioRxiv - Microbiology 2022Quote: Infectious clone plasmids were linearized using AscI (New England Biolabs) and then in vitro transcribed using the MEGAscript SP6 transcription kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: The TALEN encoding plasmids were linearized with NotI (NEB R0189S), which cut once downstream of the TALEN ORF ...
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bioRxiv - Developmental Biology 2022Quote: Plasmid with iTol2 sequence linearized using Not1 restriction enzyme (NEB). Linearized DNA was used as a template and RNA synthesis was performed using the HiScribe SP6 RNA Synthesis Kit (NEB ...
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bioRxiv - Immunology 2022Quote: ... The resulting pAF300 plasmid was linearized with PmeI (NEB, R0560S) and then transformed into Escherichia coli BJ5183 cells containing the E1/E3-deleted pAdEasy Ad5 vector backbone (Figure 1 ...
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bioRxiv - Developmental Biology 2022Quote: ... the pCS2-zswim5 plasmid was linearized with NotI-HF (NEB) and purified using a Wizard SV Gel and PCR Clean-up System (Promega) ...
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bioRxiv - Neuroscience 2020Quote: ... The parent plasmid was treated with BamHI (NEB, Ipswich, MA) and EcoRI (NEB ...
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bioRxiv - Biophysics 2021Quote: ... were produced by linearization of large plasmids with NotI (NEB). Without further purification ...
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bioRxiv - Bioengineering 2020Quote: Plasmids were linearized with Not-I HF (New England Biolabs) overnight at 37°C ...
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bioRxiv - Genomics 2022Quote: pUC19 plasmid was digested with SmaI (New England Biolabs, R0141S) and the linearized plasmid was gel extracted with the QIAquick Gel Extraction Kit (Quiagen ...
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bioRxiv - Immunology 2022Quote: ... plasmid 17398) using Gibson Assembly Master Mix (New England BioLabs). After sequence validation by Sanger sequencing ...
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bioRxiv - Biochemistry 2022Quote: ... plasmids were digested with BamHI-HF (New England Biolabs R3136S)/MfeI-HF (New England Biolabs R3589S ...
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The plant pathogen Pectobacterium atrosepticum contains a functional formate hydrogenlyase-2 complexbioRxiv - Microbiology 2019Quote: All plasmids were cloned using Gibson assembly (HiFi Assembly, NEB) using DNA amplified from P ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... SNAP-tag comes from pSNAPf-ADRβ2 control plasmid (NEB, #N9184). SNAP-CB2-TwinStrep-1D4 ...
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bioRxiv - Molecular Biology 2019Quote: ... Ligated plasmids were transformed into BL21 (DE3) cells (C2527, NEB) and correct clones verified by Sanger sequencing ...
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bioRxiv - Neuroscience 2020Quote: Tol2 transposase plasmid (pCS-TP) was linearised using Not1 (NEB) and mRNA was transcribed in vitro using the mMESSAGE mMACHINE SP6 Kit (Ambion Inc.) ...
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bioRxiv - Molecular Biology 2021Quote: ... These plasmids were linearized with Pme I (New England Biolabs) and transformed in strain DPY034 for integration at the TRP1 locus to obtain strains DPY1805 and DPY1818 ...
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bioRxiv - Microbiology 2021Quote: ... The N expression plasmid was linearized using EcoRV-HF (NEB). Capped-RNA was produced using the mMESSAGE mMACHINE T7 Transcription Kit (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: ... The acceptor plasmid was cut with NheI (New England BioLabs) between the end of KDM5B and the MS2 stem loops ...
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bioRxiv - Biochemistry 2020Quote: ... The acceptor plasmid was cut with NheI (New England BioLabs) between the end of KDM5B and beginning of SunTag ...
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bioRxiv - Biochemistry 2020Quote: ... The acceptor plasmid was cut with NotI (New England BioLabs) at the beginning of SM ...
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bioRxiv - Biochemistry 2020Quote: ... The acceptor plasmid was cut with AscI (New England BioLabs) between the end of Kif18b and beginning of SM ...
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bioRxiv - Cell Biology 2020Quote: The parental plasmid was digested with the DpnI enzyme (NEB). The mCherry was then replaced by eGFP to generate the RUSH CD63-YA-eGFP plasmid ...
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bioRxiv - Biochemistry 2021Quote: ... Whole-plasmid amplification reactions were performed using Phusion polymerase (NEB) and Ampligase (Epicentre) ...
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bioRxiv - Synthetic Biology 2021Quote: ... treatment and DNA purification with Monarch Plasmid Miniprep kit (NEB), or (ii ...
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bioRxiv - Microbiology 2020Quote: ... replicon plasmid DNA linearized with AscI (NEB, Ipswich, Massachusetts, USA) was used to template T7 in vitro transcription reactions ...
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bioRxiv - Microbiology 2021Quote: ... Plasmid construction was carried out using enzymes obtained from NEB (NEBuilder HiFi DNA assembly master mix ...
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bioRxiv - Microbiology 2021Quote: ... of existing plasmids or HiFi Gibson Assembly (New England Biolabs) of linear dsDNA fragments ...
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bioRxiv - Genetics 2020Quote: ... ESCs were electroporated with the linearized plasmid (Fsp1 NEB R0135) in the presence of hygromycin and individual clones were isolated as we have done previously and expanded for further experiments.
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bioRxiv - Genetics 2020Quote: ... Ligated plasmids were transformed into NEB High Efficiency (NEB, C2987) bacteria ...
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bioRxiv - Molecular Biology 2021Quote: ... The pLentiGuide-Blast plasmid was digested with BsmBI-V2 (NEB) at 55°C for 8h followed by 20 min heat inactivation at 80°C and gel purified using the QIAEX II Gel Extraction Kit (Qiagen) ...
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bioRxiv - Genomics 2022Quote: pUC19 plasmid was digested with SmaI (New England Biolabs, R0141S) and the linearized plasmid was gel extracted with the QIAquick Gel Extraction Kit (Quiagen ...
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bioRxiv - Microbiology 2022Quote: ... The minipreped plasmids were cut by HindIII (NEB cat. R3104S), and gel electrophoresed.
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bioRxiv - Plant Biology 2022Quote: ... and introduced into binary plasmids using NEBuilder Hifi assembly (NEB) (Figure 1a) ...
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bioRxiv - Immunology 2022Quote: ... plasmids were linearized with PstI (New England Biolabs, MA, USA) and purified using a PCR purification kit (Roche ...
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bioRxiv - Microbiology 2023Quote: ... The plasmid was first linearized by EcoRV (New England Biolabs), and then transfected into VSG2-expressing cells (2T1) ...
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bioRxiv - Molecular Biology 2023Quote: ... The parental plasmid was digested with 5U DpnI (NEB, R0176S). Plasmid transformation was achieved by using the heat shock method (42°C ...
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bioRxiv - Biophysics 2023Quote: ... Plasmids were linearized using Nhe I HF (New England Biolabs) and transcribed in vitro (mMessage-mMachine T7 Kit ...
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bioRxiv - Developmental Biology 2023Quote: ... The final donor plasmid was generated through Gibson cloning (NEB) of a synthetic FseI-HA-KRAB-AscI DNA fragment (GenScript ...
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bioRxiv - Cell Biology 2023Quote: ... we linearized the plasmid for genomic integration using AflII (NEB), a silent mutation inserted previously.
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bioRxiv - Bioengineering 2023Quote: ... The Csy3-VPR-Csy4 plasmid was digested with MluI (NEB) and BamHI (to remove the VPR TAD ...
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bioRxiv - Immunology 2023Quote: ... plasmids using T4 DNA ligase (New England BioLabs, cat. M0202S). Sanger sequencing confirmed successful cloning.
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bioRxiv - Biochemistry 2023Quote: ... Assembled plasmids were transformed into NEB 5-alpha F’Iq (NEB) and standard chemically competent Escherichia coli transformation protocols were used to construct plasmid host strains ...