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Citations for New England Biolabs :
101 - 150 of 976 citations for Dl 4 Methoxyestradiol 13 14 15 16 17 18 13C6 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Ligated junctions were pulled-down with Dynabeads® MyOne™ Streptavidin C1 beads for 15 min at RT and DNA ends were A-tailed with 15 Units of Klenow exo- (cat. M0212L, NEB). Barcoded PerkinElmer adapters (cat ...
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bioRxiv - Cell Biology 2023Quote: ... Ligated junctions were pulled-down with Dynabeads® MyOne™ Streptavidin C1 beads for 15 min at RT and DNA ends were A-tailed with 15 Units of Klenow exo- (cat. M0212L, NEB). Barcoded PerkinElmer adapters (cat ...
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bioRxiv - Microbiology 2021Quote: ... for 16 cycles using Universal primer and unique indices (NEB Catalog# E7335L, E7500L) in a total of in a 50 μl reaction volume ...
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bioRxiv - Microbiology 2022Quote: Eight units of enteropeptidase light chain (New England Biolabs, 16 units per µL) and 25 µg of Esp743 (50 µg/µL ...
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bioRxiv - Genomics 2022Quote: ... ligated overnight at 16°C and subsequently transformed into competent cells (NEB C2992H).
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bioRxiv - Plant Biology 2023Quote: ... and ligated overnight at 16°C using T4 ligase (NEB, Ipswich, Massachusetts, USA). The fully reassembled BdPOX gene was ligated overnight at 16°C with the previously digested (FastDigest Eco31I ...
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bioRxiv - Synthetic Biology 2019Quote: ... and 15 units of T4 PNK enzyme (NEB). The reaction was incubated at 37 °C for two hours and PAGE–purified.
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bioRxiv - Bioengineering 2020Quote: ... and 15 uL of H2O) (New England Biolabs) for 1 hour at 37°C followed by DNase I (10 µL DNase I ...
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bioRxiv - Genomics 2023Quote: ... 15 μL 2x Q5U Master Mix (NEB M0597S), 0.4 μL 100 μM Nextera P5 index primer and 0.4 μL 100 μM Nextera P7 index primer ...
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bioRxiv - Genomics 2024Quote: ... and 15 U Klenow exo (New England Biolabs), for 30 min at 37°C before inactivation for 20 min at 65°C ...
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bioRxiv - Genomics 2024Quote: ... 15 U T4 polynucleotide kinase (New England Biolabs), 5 U Klenow DNA polymerase (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... 2.5µL of 10µM MBTUni-12 primer + 2.5µL of 10µM MBTUni-13 primer (5’-ACGCGTGATCAGTAGAAACAAGG-3’) + 10µL 5x HF Phusion Buffer + 1µL 10mM dNTPs mix (NEB #N0447S) + 0.5µL Phusion Polymerase + 28.5µL of Nuclease-free water (Ambion ...
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bioRxiv - Genetics 2019Quote: ... and 2.0 units of the CspCI enzyme ((N)10-11CAA(N)5GTGG(N)12-13) (New England Biolabs). The library preparation was carried out according to the protocol proposed by Osorio-Guarín et al ...
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bioRxiv - Biochemistry 2023Quote: ... 13 pmol of purified RNA was phosphorylated with y-32P ATP (Hartmann Analytics) by T4 polynucleotide kinase (NEB) for 30 min at 37 °C ...
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bioRxiv - Plant Biology 2020Quote: ... Resulting fragments were amplified for 14 cycles using NEBNext Ultra II PCR protocol (New England BioLabs) and quality was assessed with the Agilent High sensitivity DNA kit ...
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bioRxiv - Plant Biology 2021Quote: ... and (iii) using 18 cycles of PCR amplification with Phusion High Fidelity DNA Polymerase (New England Biolabs). Libraries were pooled and sequenced (paired-end 2×150bp ...
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bioRxiv - Biophysics 2021Quote: ... Complexes were prepared by incubating spike ectodomain preparations with either ACE2 (residues 18–615, New England Biolabs), VH ab8 ...
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bioRxiv - Genomics 2020Quote: The 254bp V4 segment of the 16S ribosomal RNA subunit was amplified by a 17-cycle touchdown PCR using the following protocol: 12.5 μl Phusion 2X Master Mix (New England BioLabs),1 μl each of 16S V4 forward and reverse primers (primer pair 515F-806R) ...
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bioRxiv - Neuroscience 2020Quote: We selected 17 sSNVs identified in brain-active enhancers and attempted cloning and site-directed mutagenesis (New England Biolabs) to recreate the mutations ...
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bioRxiv - Immunology 2023Quote: pals-17::gfp and pals-20::wrmScarlet translational reporter constructs were assembled using NEBuilder HiFi kit (New England Biolabs) by fusing PCR products and gene fragments ...
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bioRxiv - Developmental Biology 2023Quote: RNA was isolated from 17 hpf Ciona robusta larvae by Monarch Total RNA miniprep kit (New England BioLabs Inc.). cDNA was produced by Omniscript reverse transcription kit (QIAGEN) ...
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bioRxiv - Molecular Biology 2021Quote: ... Ligation was conducted overnight at 16 °C with T4 DNA Ligase (New England Biolabs). All minigene mutations were introduced via Q5 Site-Directed Mutagenesis Kit (New England Biolabs) ...
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bioRxiv - Genomics 2020Quote: ... with BspQ1 overhangs were ligated using T7 DNA ligase (NEB M0318; 16°C overnight) into BspQ1 digested (NEB R0712 ...
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bioRxiv - Molecular Biology 2022Quote: ... and ligated at 16 °C with 10,000 U of T4 DNA ligase (NEB, M0202) in ligase buffer supplemented with 0.8% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... The amplified backbone was treated with Dpn1 for 16 hours at 37°C (NEB) followed by a purification using a spin column ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysates were additionally treated with 16 units of PNGase F (New England Biolabs) per μL of lysate complemented with 1% NP-40 ...
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bioRxiv - Biophysics 2023Quote: ... the buffer was supplemented with 16 mM ATP and RecA (New England Biolabs, USA) as well as SSB gradients were added to the respective rows (Tab ...
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bioRxiv - Microbiology 2023Quote: ... 250 μL of sample with 16 μg/mL TPCK-treated trypsin (New England BioLabs), 2X penicillin and streptomycin and 2X antibiotic-antimycotic (Wisent ...
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bioRxiv - Systems Biology 2021Quote: ... the beads were used to amplify libraries using 13 cycles of PCR with the Illumina Forward PE1.0 primer and Illumina Reverse indexed primer (New England Biolabs) in the presence of Kapa HiFi HS DNA Polymerase (Kapa Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: ... and a PCR with 10-13 cycles was performed using the NEBNext High Fidelity 2X PCR Master Mix (NEB) and Ad1_noMX and Ad2.1–2.12 barcoded primers described in (30) ...
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bioRxiv - Cell Biology 2024Quote: ... 16.4 mL elution was taken into 40 mL ligation reaction (13 Quick ligase buffer [NEB], 4000U Quick ligase [NEB] ...
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bioRxiv - Cell Biology 2021Quote: ... 14 μg of sample DNA were digested overnight at 25°C with CviQI restriction enzyme (NEB, R0639L), which cuts outside the region of interest.
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bioRxiv - Molecular Biology 2024Quote: ... 1.5 ul 10 mM dGTP, 1.5 ul 10 mM dTTP, 37.5 ul 0.4 mM biotin-14-dATP, 40 ul NEB buffer2 ...
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bioRxiv - Genomics 2021Quote: ... and 15 units of Phi29 polymerase (New England Biolabs). We used a PCR machine (SimpliAmp ...
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bioRxiv - Molecular Biology 2022Quote: ... 15 U T4 RNA Ligase High Concentration (M0437, NEB)) were added as well as 10 μl 50% PEG8000 and reaction was mixed by pipetting up- and down until beads are resuspended ...
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Removal of Spo11 from meiotic DNA breaks in vitro but not in vivo by Tyrosyl DNA Phosphodiesterase 2bioRxiv - Molecular Biology 2019Quote: ... 15 units of Klenow (3′→5 ′ exo–) polymerase (NEB) with 66 nM dNTPs for 30 minutes at 30°C ...
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bioRxiv - Neuroscience 2022Quote: ... 15-20 µL of amylose beads (New England Biolabs) were mixed with (MBP)2-WRC (bait ...
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bioRxiv - Genomics 2023Quote: ... 15 units of RNase H1 (NEB, cat. no. #M0297) or 1 μg/μL RNase A (Life Technologies ...
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bioRxiv - Genomics 2023Quote: ... and 15 units of Phi29 polymerase (New England Biolabs). We used a PCR machine (SimpliAmp ...
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bioRxiv - Neuroscience 2020Quote: ... Total RNA was extracted from the heads of 20 male adults or 17 female adults using a Monarch Total RNA Miniprep Kit (NEB). 3 replicates for male and 3 replicates for female flies were done for each genotype ...
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bioRxiv - Plant Biology 2020Quote: ... 17 μl of the PCR reaction were mixed with 2 μl of CutSmart Buffer and 1 μl of AvaI (NEB) for a final volume of 20 μl ...
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bioRxiv - Microbiology 2020Quote: ... Circularised product is subjected to two rounds of rRNA depletion using biotinylated oligos [17] and streptavidin-coated magnetic beads (NEB) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... The rRNA-subtracted circular product is subjected to a final round of PCR amplification with Illumina adaptor primers [17] using Phusion polymerase (NEB). All the libraries were then quantified ...
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bioRxiv - Cell Biology 2023Quote: ... sgRNA cassettes were isolated by two rounds of PCR using a previously published strategy (17) with NEBNext Ultra II Q5 Master Mix (New England Biolabs) and primer pairs listed in Extended Data Table 2.
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bioRxiv - Plant Biology 2019Quote: ... the corresponding dsRNAs were processed in 18-25 nt siRNAs by ShortCut® RNase III (NEB, Ipswich, MA) for 40 min at 37°C ...
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bioRxiv - Genetics 2022Quote: ... Sequencing libraries were prepared with NEBnext Ultra DNA Library Prep Kit (New England Biolabs; version 6.0 – 2/18) using NEBNext Multiplex Oligos for Illumina-Dual Index Primers Set 1 (#E7600S ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 50 μl of DNase I mixture (25 μl of 10× DNase I buffer, 18 μl of NEB DNase I ...
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bioRxiv - Neuroscience 2021Quote: ... and PCR amplified for 13 cycles with Illumina Nextera adapter primers using the NEBNext High Fidelity 2X Master Mix (NEB) with the following PCR program ...
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bioRxiv - Molecular Biology 2022Quote: ... regions flanking each target site were PCR amplified using locus-specific primers bearing tails complementary to the TruSeq Illumina adapters as described previously.13 25-50ng input genomic DNA is PCR amplified with Q5 High Fidelity DNA Polymerase (New England Biolabs): (98°C ...
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bioRxiv - Immunology 2022Quote: ... In vitro transcription (IVT) was performed at 37°C for 13-16h with the HiScribe T7 RNA Polymerase kit (NEB). The remaining DNA was digested by Turbo DNase I (Life Technologies ...