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Citations for New England Biolabs :
351 - 400 of 976 citations for Dl 4 Methoxyestradiol 13 14 15 16 17 18 13C6 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... A 7:1 molar ratio of barcode oligonucleotide to sublibrary was ligated overnight at 16°C with T4 DNA ligase (New England Biolabs).
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bioRxiv - Neuroscience 2024Quote: ... The final cDNA sample was then split into 16 separate reactions for final PCR amplification using 16 unique Illumina indexing primers and Q5 Hot Start High-Fidelity 2X Master Mix (NEB). After amplifications ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were diluted in 8 ml of T4 DNA ligase buffer 1X and incubated 8 hours at 16°C with 8000 Units of T4 DNA ligase (NEB). Crosslinks were reversed overnight at 60°C in the presence of proteinase K (0.125 mg / ml final ...
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bioRxiv - Molecular Biology 2021Quote: ... and 15 μL of 2× Phusion High-Fidelity PCR Master Mix (New England Biolabs). All PCR amplification reactions were carried out on a T100 Thermal Cycle (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: ... Lysates pre-cleared for 15 minutes with 50µl goat anti-rabbit magnetic beads (NEB). Pre-cleared lysates were then incubated with either goat anti-rabbit magnetic beads only (mock IP ...
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bioRxiv - Microbiology 2022Quote: ... 15 μl 20% SDS and 3 μl 20 mg/ml Proteinase K (NEB #P8107) was added to the sample and the mixture was incubated at 37 °C for 30 min with agitation at 1400 rpm to completely lyze the cells ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were labeled for 15 min with 2 μM TMR-Star (New England Biolabs) in complete medium for pulse labeling ...
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bioRxiv - Genomics 2020Quote: ... ddPCR absolute quantification was performed using 15 ng of DraI or AluI (NEB, US) pre-restriction digested DNA in a 20ul reaction mix containing 1x ddPCR Supermix for Probes (BioRad ...
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bioRxiv - Cell Biology 2020Quote: ... supernatant was discarded and fill-in mix was added (38 μM Biotin-14-dATP [Thermo Fisher Scientific], 38 μM dCTP, dGTP and dCTP [Thermo Fisher Scientific], 50 U Klenow Polymerase [NEB], 1x NEB 2 buffer) and cells incubated for 1 h at 37 °C under rotation ...
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bioRxiv - Cell Biology 2020Quote: ... nuclei were spun down and resuspended in fill-in mix (biotin-14-dATP [Thermo Fisher Scientific], dCTP, dGTP and dTTP [Thermo Fisher Scientific], Klenow Polymerase [NEB], 1x NEB 2 buffer) for 1.5 h at 37°C with rotation ...
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bioRxiv - Bioengineering 2021Quote: ... 4 µg plasmid was digested for 4 h at 37°C with BstXI and XhoI (New England Biolabs). Products were run on a 1% agarose gel for 30 min at 120 V ...
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bioRxiv - Cancer Biology 2019Quote: ... Paired end strand-specific whole transcriptome libraries were prepared for 16 pairs using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs, UK), following rRNA depletion with NEBNext rRNA Depletion Kit or Ribo-Zero Gold ...
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bioRxiv - Biochemistry 2020Quote: ... The dried glycoproteins were incubated with trypsin for 16 h at 37°C before releasing N-glycans using PNGase F (New England Biolabs, MA). Liberated N-glycans were separated from glycopeptides by C18 Sep Pak SPE cartridges (Waters Corporation ...
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bioRxiv - Microbiology 2022Quote: ... The following day swab samples were vortexed and spun down and 200 ul of the swab samples medium was combined with 16 ug/ml working concentration of TPCK-treated trypsin (New England Biolabs Ltd), 2x A/A/p/s antifungal/antibiotic solution (Wisent) ...
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bioRxiv - Zoology 2020Quote: The 16S rRNA and gltA amplicons from PCR assays were cleaned with the ExoSAP-IT kit (E1050, New England Biolabs, US) and Sanger sequenced ...
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bioRxiv - Cell Biology 2021Quote: ... HindIII and BamHI restriction sites were inserted just after codon coding for Proline 16 using the Q5-insertion mutagenesis (NEB, #E0554) according to the protocol from the manufacturer ...
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bioRxiv - Systems Biology 2023Quote: ... The first round was performed with primer pairs listed in Supplementary Table S7 with 16 cycles using NEBNext High Fidelity Master Mix (NEB, # M0541) for the screens with EpiC and Tiling libraries and NEBNext Ultra II Q5 Master Mix (NEB ...
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bioRxiv - Microbiology 2023Quote: ... The unique barcoded region was amplified from 500ng genomic DNA with 16 cycles of PCR using NEBNext Ultra II Q5 master mix (NEB M0544L) as described in30 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... We prepared genomic DNA libraries for the 16 samples using NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs, Inc). We quantified the quality of the genomic DNA library using the Agilent 2100 Bioanalyzer system located at the Genome Analysis Core Laboratories at Georgia Institute of Technology (Agilent Technologies ...
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bioRxiv - Genomics 2022Quote: ... We then ordered an oligo containing 16 Ns with flanking homology arms to the landing pad plasmid (GWLP P1) and used HiFi Assembly (NEB #E2621) to assemble the oligo to the plasmid (50℃ ...
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bioRxiv - Microbiology 2023Quote: ... Two μl of a 1:250 dilution of the annealed oligos were ligated to 50 ng of dephosphorylated BsaI-digested vector at 16°C overnight in 20 μl final volume containing 1x T4 DNA ligation buffer (NEB, B0202S), and 400U T4 DNA ligase (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 U of T4 DNA polymerase (NEB), 13.5 U of T4 Polynucleotide Kinase (NEB ...
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bioRxiv - Physiology 2019Quote: ... 4 μl of 10% NP-40 (NEB) and 8 μl of PNGase F (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 µL RNase H (New England Biolabs), 644 µL buffer A and 2 µL DTT (1M) ...
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bioRxiv - Biochemistry 2021Quote: ... and 4 U murine RNase inhibitor (NEB). TMAO was adjusted to pH 7.5 in a 6 M stock solution with HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... and 4 µL 10X CutSmart Buffer (NEB) in a 40 µL reaction ...
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bioRxiv - Synthetic Biology 2022Quote: ... 4 μL of Quick CIP (NEB, M0508S) was spiked into the reaction and incubated at 37°C for 30 minutes to dephosphorylate unincorporated dNTPs that may inhibit downstream processes ...
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bioRxiv - Synthetic Biology 2022Quote: ... 4 μL of T5 Exonuclease (NEB M0663S) was added to the reaction and incubated at 37°C for 30 minutes to remove unassembled products ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.1 μl 10X NEB 4 buffer (NEB), 0.1 μl 10 mM dCTP and H2O ...
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bioRxiv - Biophysics 2022Quote: ○ 4 μL Klenow Exo-enzyme (NEB #M0212S) μL
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bioRxiv - Microbiology 2024Quote: ... 4 µl of 10x Apyrase buffer (NEB) and 1 µl of Apyrase (M0398S ...
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bioRxiv - Microbiology 2024Quote: ... 4 µl of 10x r3.1 buffer (NEB), 2 µl of 100µM DTT and 1 µl of NudC (M0607S ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA products were then amplified for 15 cycles of PCR using longAmp Taq (NEB M0287S). During amplification PrepX RNAseq index barcode primers were added for each library to enable multiplexing ...
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bioRxiv - Biophysics 2021Quote: ... 15 µg of the purified nicked minicircle was incubated with T4 ligase (New England Biolabs) and ligase buffer containing 25 µg mL-1 BSA ...
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bioRxiv - Evolutionary Biology 2020Quote: ... samples were digested for 15 min at 37 °C with 5 μL of ExoI (NEB) to remove first round primers ...
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bioRxiv - Genomics 2021Quote: ... The template was PCR amplified (Taq 2x master mix, NEB M0270; Bridge primers; 15 cycles) and size selected by 2% agar gel.
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bioRxiv - Biochemistry 2022Quote: Lysates were incubated with 15 μL of lysis buffer-equilibrated amylose resin (New England Biolabs) for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 0.1 mM dATP and 15 units of Klenow Fragment (3’→5’ exo-) (NEB, M0212M) and incubated at 37°C for 30 min ...
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bioRxiv - Biochemistry 2022Quote: Oligonucleotides (1.0 μM) were incubated with 15 units of T4 PNK (New England BioLabs, M0202S) and 200 μM 0.555 MBq γ-32P-ATP (185 TBq/mmol ...
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bioRxiv - Genomics 2021Quote: ... Debranching was performed by incubation with 15 units of T7 endonuclease I (New England Biolabs) for 15 minutes at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... cDNA libraries were obtained by PCR amplification for 15 cycles using Phusion DNA polymerase (NEB). After quantification with a micro fluorometer (TBS-380 ...
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bioRxiv - Molecular Biology 2023Quote: ... a self-packed XK column (Cytiva) with 15 mL of Amylose resin (New England Biolabs) was attached to the HisTrap column ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated for 15 minutes in the presence of concentrated T4 DNA Ligase (NEB-M0202M). Finally the ligated RNA:DNA hybrid was purified using 1X Agencourt RNAClean XP beads ...
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bioRxiv - Biophysics 2023Quote: ... The mixture was then combined with 15 uL 10 mM dNTPs (New England Biolabs; #N0447S) and 1 uL SUPERase-In (ThermoFisher AM2696 ...
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bioRxiv - Genetics 2021Quote: ... Supernatant containing genomic DNA was used as a template for the PCR amplification of the 16S rRNA gene using Taq polymerase (New England Biolabs, Ipswich, MA) and the primers oMC98f (5’-GAAGAGTTTGATCATGGCTC-3’ ...
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bioRxiv - Genomics 2020Quote: ... These were ligated to modified Illumina P1 and P2 adapters overnight at 16 °C with 1,000 units of T4 ligase (New England Biolabs, Beverly, MA, USA), followed by a 10-minute heat-deactivation step at 65 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Enzyme restriction with DpnII was performed in 30 µl reaction mix (1 µl DpnII, 3 µl DpnII buffer, 10 µl purified DNA and 16 µl H2O; New England Biolabs, Ipswich, MA) and incubation at 37°C for 2h ...
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bioRxiv - Molecular Biology 2022Quote: 16S rDNA inputs reacted with two self-looping oligonucleotides (SLOs) in NEBuilder® HiFi DNA Assembly Master Mix (New England BioLabs®) at 45°C for 2-3 hours ...
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bioRxiv - Microbiology 2019Quote: ... A high fidelity PCR was performed with the same 16S primers but using Q5 Hot start 2x master mix (New England Biolabs, Hitchin, U.K.). After amplification ...
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bioRxiv - Microbiology 2023Quote: Amplification of the V3-V4 hypervariable regions of the 16S rRNA gene was carried out using the Q5 high-fidelity DNA polymerase (New England Biolabs, Ipswich, USA). The PCRs were performed in 50 µl reactions ...