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Citations for New England Biolabs :
4651 - 4700 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... and used for the cloning of PCR fragments with a Gibson Assembly cloning kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... the HiScribe® T7 High Yield RNA Synthesis Kit from New England Biolabs (NEB E2040S, Ipswich, MA) was used with the standard RNA synthesis protocol and incubated for 4 hours ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and lysed using the Tissue Lysis buffer from the Monarch® Genomic DNA Purification Kit (NEB, #T3010S) with double the volume of lysis buffer and a 1 h incubation at 56 C ...
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bioRxiv - Neuroscience 2023Quote: ... and Cttn-VC155 were generated by NEBuilder® HiFi DNA Assembly Cloning Kit (# E5520S, New England BioLabs), which enables the assembly of multiple inserts within a linearized vector in a one-step PCR reaction ...
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bioRxiv - Neuroscience 2023Quote: ... After quantification of individual libraries by q-PCR using the NEBNext library quant kit (New England BioLabs), all the samples were pooled in an equimolar manner ...
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bioRxiv - Microbiology 2023Quote: We performed RT-qPCR on select genes using the Luna Universal One-Step Kit (New England Biolabs) and QuantStudio 5 (Thermo) ...
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bioRxiv - Genetics 2024Quote: ... Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA, Catalog #: E7530L) following the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2024Quote: ... The amplified pools were subjected to PCR product purification by Monarch PCR and DNA Cleanup Kit (NEB). The purified cDNAs were cloned by NEB PCR Cloning Kit and individual clones were isolated and sequenced (Azenta Life Sciences).
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bioRxiv - Cancer Biology 2024Quote: ... GAPF libraries were constructed with the NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs), which employs fragmentase for DNA fragmentation ...
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bioRxiv - Synthetic Biology 2024Quote: ... The variants were created from the wild type plasmids using the Q5 Site-Directed Mutagenesis Kit (NEB) and transformed into MegaX cells made chemically competent (Mix & Go E ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were submitted to Novogene for library preparation (NEB Next® Ultra™ RNA Library Prep Kit) and paired-end (150bp ...
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bioRxiv - Neuroscience 2024Quote: ... and cDNA was synthesized using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, #E6560L). cDNA was amplified using primer pairs designed to either target two wildtype sequences on either side of the cryptic exon or one wildtype sequence and another sequence directly in the cryptic exon ...
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bioRxiv - Neuroscience 2024Quote: RNA was extracted from samples using the Monarch® Total RNA Miniprep Kit (New England Biolabs, #T2010S) and cDNA was synthesized using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted using the Monarch genomic DNA extraction and purification kit (New England Biolabs Inc.). Region of Cori and ter were quantitatively amplified using the Sybr green-based Luna Universal One-step RT-qPCR kit (New England Biolabs Inc.) ...
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bioRxiv - Microbiology 2023Quote: ... ChIP-sequencing libraries were generated using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) and sequenced with an Illumina HiSeq2500 (50 bp single-end) ...
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Multidrug resistance plasmids commonly reprogramme expression of metabolic genes in Escherichia colibioRxiv - Microbiology 2023Quote: ... Genomic DNA was extracted from overnight cultures using the Monarch Genomic DNA Purification Kit (New England Biolabs). DNA was quantified using a Qubit 4 fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... The library was quantified using the NEBNext® Library Quant Kit for Illumina® (New England Biolabs) by mixing 2 ul 1:10.000 diluted library with 8 ul Quant mastermix with added primers (New England Biolabs) ...
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bioRxiv - Microbiology 2023Quote: ... Colonies were prepared for whole genome sequencing using an Epicentre Masterpure DNA Preparation Kit (Nordic Biolabs, Sweden). Genomic DNA libraries were constructed using Nextera XT Library Preparation Kit (Illumina ...
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bioRxiv - Immunology 2023Quote: ... IVT was done using the HiScribe® T7 High-Yield RNA Synthesis Kit (New England Biolabs, #E2040S), with substitution of N-1-methyl-pseudouridine-5’-triphosphate (TriLink Biotechnologies ...
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Regulation of transcription patterns, poly-ADP-ribose, and RNA-DNA hybrids by the ATM protein kinasebioRxiv - Molecular Biology 2023Quote: Total RNA extraction was performed using NEB Monarch Total RNA Miniprep Kit (New England Biolabs Cat #T2010S). RNAseq library prep was performed using NEBNext Ultra II RNA Library Prep for Illumina (NEB Cat# E7770S) ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA library was prepared using the NEBnext Ultra II DNA Library Prep Kit (New England Biolabs, E7645) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, Cat #E7645) following manufacture’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The sequencing library was constructed using an NEBNext rRNA Depeletion Kit (Bacteria) (New England Biolabs, Ipswich, MA). From the kit ...
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bioRxiv - Microbiology 2023Quote: ... which was transcribed in vitro using the HiScribe T7 high-yield RNA synthesis kit (New England Biolabs). Afterwards ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting gRNAs were purified using the AMPure XP kit and treated with DNase I (NEB, UK). Concentration was evaluated on a Qubit system (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA seq libraries were constructed from total RNA using the NEBNext Ultra II Directional RNAseq kit (NEB) with ribosomal RNA depletion ...
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bioRxiv - Neuroscience 2023Quote: ... and subsequently prepared for RNA-seq with the NEBNext Ultra Directional RNA Library Prep Kit (NEB #E7420) at GenomeScan ...
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bioRxiv - Microbiology 2023Quote: cDNA was synthesized from extracted viral RNA using the LunaScript® RT SuperMix Kit (NEB, Ipswich, MA) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... 4NBX.B WT sequence with C103A mutation was created by site-directed mutagenesis using commercially available kits (NEB). Smt3 SUMO protein with an N-terminal cysteine was created from wild type Smt3 SUMO by mutagenic PCR ...
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bioRxiv - Cell Biology 2023Quote: ... Exon library was constructed using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (NEB). DNA library hybridization was performed by using Agilent SureSelect Human All Exon V6 (Agilent) ...
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bioRxiv - Cancer Biology 2023Quote: ... NGS libraries were prepared using NEBnext Ultra II DNA library preparation kit for Illumina (New England Biolabs) and quantified with using a TapeStation (Agilent Genomics) ...
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bioRxiv - Biophysics 2023Quote: ... Total RNA was extracted from cell cultures using the Monarch Total RNA Miniprep Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: Strand-specific RNA libraries were generated using the NEBNext Ultra II stranded library kit (New England Biolabs) combined with polyA-coupled beads (New England Biolabs ...
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bioRxiv - Biochemistry 2023Quote: ... The SKP1-FBXO22 mutant (C326A) was generated using a Q5 Site-Directed Mutagenesis Kit (NEB, cat. E0552). Both SKP1-FBXO22 WT and C326A were expressed in BL21(DE3 ...
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bioRxiv - Bioengineering 2023Quote: Complementary DNA (cDNA) was synthesized using the First Strand cDNA Synthesis Kit (New England BioLabs, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Then first-strand cDNA was produced using ProtoScript® II First Strand cDNA Synthesis Kit (NEB, E6560s) with random primers ...
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bioRxiv - Evolutionary Biology 2023Quote: ... complemented by reagents from the NEBNext Ultra II library prep kit (New England Biolabs, Ipswich, Massachusetts, U.S.A.), and then sequenced at a MinION platform (Oxford Nanopore Technologies ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from extracted RNA samples using M-MuLV reverse transcriptase kit (New England Biolabs Ltd) based on the manufacturer’s instructions and using customized primers splice leader (SL_F ...
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bioRxiv - Cancer Biology 2023Quote: ... First strand cDNA synthesis was performed with LunaScript RT SuperMix Kit (E3010L; New England Biolabs, Ipswich, MA), and 100 ng-1 μg total RNA was used for one 20 μl RT reaction ...
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bioRxiv - Biochemistry 2024Quote: ... All individual PLpro mutations were introduced into pDONR221-PLpro using NEB Q5 Site-Direct Mutagenesis Kit (NEB) following manufacturer’s instructions and shuttled into FU-tetO-Gateway-rtTA-2A-Puro using LR clonase.
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bioRxiv - Genetics 2023Quote: ... and genomic DNA libraries were prepared using the NEBNext genomic DNA library construction kit (New England Biolabs). DNA libraries were sequenced on an Illumina Hi-Seq and deep sequencing reads were analyzed using standard methods on Galaxy ...
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bioRxiv - Developmental Biology 2023Quote: ... Isolated gDNA was then prepared for epigenetic sequencing using the Enzymatic Methylation Sequencing Kit (EM-Seq) (NEB).49 Individual embryos were sequenced at a depth of 100 million reads using a 2 × 150 bp Illumina kit on the Illumina NovaSeq instrument ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were built with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer instruction ...
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bioRxiv - Immunology 2023Quote: ... Immunoprecipiated DNA was used to prepare libraries using NEBNext Ultra II DNA library prep kit (NEB E7645S). Libraries were sequenced with paired-end 50 on an Illumina Novaseq.
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bioRxiv - Cancer Biology 2023Quote: ... The RNA-seq libraries were prepared with Ultra II Directional RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was reverse transcribed from 1 µg of total RNA using LunaScript RT Supermix kit (NEB #E3010) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Molar concentration of libraries was determined using NEBNext® Library Quant Kit for Illumina® (NEB, #E7630) following manufacturer’s protocol for 6 Standards ...
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Genomic epidemiology and antifungal-resistant characterization of Candida auris, Colombia, 2016-2021bioRxiv - Genomics 2023Quote: ... Genomic libraries were constructed using NEBNext Ultra DNA Library Prep kit (New England Biolabs, Ipswich, MA, USA) for Illumina and sequenced on Illumina Nova-Seq using NovaSeq 6000SP reagent kit (500 Cycles) ...
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bioRxiv - Physiology 2023Quote: ... and NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Inc., Ipswich, MA, USA), with minor modifications to the manufacturers’ protocol (Kelley et al ...
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bioRxiv - Plant Biology 2024Quote: ... RNA sequencing was executed using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations ...