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Citations for New England Biolabs :
4501 - 4550 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... The complementary DNA (cDNA) was synthesized using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs) following the manufacturer’s instructions with primer d(T)23 VN ...
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bioRxiv - Microbiology 2021Quote: ... or built directly into the plasmid of interest using the NEBuilder HiFi Assembly kit (New England Biolabs), transformed into E ...
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bioRxiv - Microbiology 2021Quote: ... crRNA was synthesized in vitro using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) by incubating the reaction at 37°C with T7 promoter primer (10μM ...
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bioRxiv - Molecular Biology 2021Quote: ... all ChIP-DNA libraries were produced using the NEBNext Ultra II DNA Library Prep Kit (NEB, E7645L) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... and in vitro synthesized RNA was subsequently purified using Monarch RNA Cleanup Kit (Cat. No. T2040S, NEB). RNA concentration was quantified by NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... followed by library preparation with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Library preparation included 11 cycles of PCR amplification ...
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bioRxiv - Microbiology 2021Quote: ... SERBP1 mutant and Vigilin mutant were obtained using the Q5® Site-Directed Mutagenesis Kit (E0554) (NEB) with deletion primers using the WT cDNA in pLVX as template ...
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bioRxiv - Immunology 2020Quote: ... (2) and (3) was synthesized through HiScribe™ T7 ARCA mRNA Kit (with tailing) (New England Biolabs). All the mRNA products were concentrated and purified via EZ-10 Spin Column RNA Cleanup & Concentration Kit (Bio Basic Inc.) ...
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bioRxiv - Microbiology 2021Quote: ... were Deletions and point mutations in HelR were created using the Q5 site directed mutagenesis kit (NEB). Primers for mutant construction were designed using the NEBaseChanger as recommended ...
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bioRxiv - Microbiology 2021Quote: ... The DNA samples were pooled into two libraries prepared with the NEBNext Ultra kit (New England BioLabs). They were sequenced on a MiSeq sequencer (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... DNA fragments were amplified by PCR and assembled by NEBuilder HiFi DNA Assembly Cloning Kit (NEB, E5520S).
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bioRxiv - Genetics 2020Quote: ... RNA was reverse transcribed to cDNA using the ProtoScript II First Strand synthesis kit (NEB, Cat# E6560S) and synthesis was performed with the “random primer” option included within the kit ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Genomics 2021Quote: ... we purified the genomic DNA from each timepoint with the Monarch gDNA extraction Kit (New England Biolabs). We then amplified the region of one of the mutations acquired by the Tf-null strain (I:1453108 C->A ...
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bioRxiv - Genomics 2021Quote: ... gDNA was extracted with the Monarch Genomic DNA purification kit (#T3010 New England Biolabs; Ipswich, MA, USA). For Oxford Nanopore sequencing of STK005 ...
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries were produced using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). Libraries were sequenced on Illumina HiSeq platform.
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bioRxiv - Genomics 2021Quote: ... Standard RNA Synthesis was carried out with the HiScribe T7 High Yield RNA Synthesis kit (NEB E2040S) for 1.5 hours according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Libraries were prepared for high-throughput sequencing using the Ultra II DNA Library Prep kit (NEB E7645L). For IP samples ...
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bioRxiv - Genomics 2020Quote: ... Plasmid libraries were harvested from liquid culture using the Monarch® Plasmid DNA Miniprep Kit (NEB #T1010). Miniprep kit reagents were scaled to accommodate one spin column for every 5mL of culture.
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bioRxiv - Microbiology 2021Quote: ... Strand-specific RNA-seq was performed using the NEBNext Ultra Directional RNA Library Prep Kit (NEB, E7420) with the following adjustments ...
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bioRxiv - Biochemistry 2020Quote: ... TSEN2H377A and TSEN34H255A point mutants were generated using the Q5 site-directed mutagenesis kit (New England Biolabs) prior to assembly into biGBac vectors ...
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bioRxiv - Cancer Biology 2021Quote: Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations with index codes added to attribute sequences to each sample ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We prepared cDNA libraries with the NEBNext Ultra RNA Library Prep Kit for Illumina (NewEngland Biolabs, MA) with poly(A ...
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bioRxiv - Molecular Biology 2021Quote: ... Non-binding mutant (F212A) was generated from template MHV-S_HE_Fc_Avitag using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs)
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bioRxiv - Plant Biology 2023Quote: MeRIP sequencing (MeRIP-seq) libraries were prepared using the Ultra II directional RNA library preparation kit (NEB) as per manufacturer’s instructions for already fragmented samples ...
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bioRxiv - Immunology 2022Quote: ... and subjected to in vitro transcription (IVT) using HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S), then treated with ExoSAP-IT (Affymetrix ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA-seq libraries were prepared using NEBNext Ultra Directional RNA library preparation kit for Illumina (NEB #E7760). Library quality was checked by Agilent BioAnalyzer 2100 using the DNA 1000 kit (Part number 5067-1504 ...
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bioRxiv - Neuroscience 2023Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... Sequencing libraries were generated using NEBNext Ultra TM RNA Library Prep Kit for Illumina (NEB, Ipswich, MA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were depleted three times of polyA(+) RNA using the Magnetic mRNA isolation kit (New England Biolabs), each time keeping the supernatant ...
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bioRxiv - Microbiology 2022Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... purified total RNA was reverse transcribed using the ProtoScript II First Strand cDNA Synthesis Kit (#E6560, NEB) and a 1:1 mix of oligodT and random hexamer primers to obtain cDNA ...
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bioRxiv - Microbiology 2023Quote: Total RNA from cell pellets was extracted using a Monarch Total RNA Miniprep kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’-phosphorylated and dA-tailed with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645). Samples were ligated to adaptor oligos for multiplex sequencing (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Transcript levels of individual genes were determined by the Luna Universal One-Step RT-PCR kit (NEB) using approximately 100-200 ng of total RNA per sample as input ...
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bioRxiv - Molecular Biology 2023Quote: ... Library preparation was done using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEW ENGLAND BioLabs) as described by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... The transcription of guide RNAs (gRNAs) was performed with HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... 400ng of total RNA was used for reverse transcription using LunaScript™ RT SuperMix Kit (NEB, USA). qPCR was performed using Forget-Me-Not™ EvaGreen® qPCR Master Mix (Biotium ...
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bioRxiv - Neuroscience 2023Quote: ... Generation of Adgrd1 N-terminal mutations was carried out using Q5 site directed mutagenesis kit (NE Biolabs) using manufacturers protocol ...
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bioRxiv - Genetics 2023Quote: ... Whole genome sequencing libraries were prepared using the NEBNext Ultra II Library kit (New England Biolabs, E7645L) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: Library construction was carried out using NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645S, NEB). 4ng of fragmented DNA was used for end-repair/A-tailing ...
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bioRxiv - Microbiology 2023Quote: ... and cDNA was generated using an AMV first strand cDNA synthesis kit (New England Biolabs, Catalog # E6550). The segment was amplified through PCR with PstI and BamHI restriction sites incorporated onto the double-stranded cDNA using specific primers (PstI-VIGS-F and BamHI-VIGS-R ...
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bioRxiv - Molecular Biology 2022Quote: ... Ligation of the insert to the vector was performed using the Quick Ligation kit (New England Biolabs). The final vector was transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... The amplicon was inserted into miniTn7/pMQ56 using the NEBuilder HiFi DNA Assembly kit (New England BioLabs) according to the manufacturer specifications ...
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bioRxiv - Microbiology 2023Quote: ... NCPF 8977 (aggregating) and NCPF 13029 (weakly-aggregating) using Monarch RNA extraction kit (NEB cat no. T2010) as per manufacturer’s specification ...
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bioRxiv - Microbiology 2022Quote: ... Reverse transcription for first-strand cDNA synthesis was performed by using the LunaScript RT SuperMix Kit (NEB) containing both oligo(dT ...
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bioRxiv - Synthetic Biology 2023Quote: ... RNA was purified using a Monarch RNA Cleanup Kit (500 μg) (Catalog No. T2050L, New England BioLabs) and concentration was measured using a NanoDrop ND-1000 UV-Vis Spectrophotometer (Thermo Scientific).
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bioRxiv - Synthetic Biology 2023Quote: ... Quick Blunting Kit and the T4 DNA Ligase enyzme were purchased from New England BioLabs (NEB, Germany). PCR was performed using Q5 High Fidelity 2X Master Mix (NEB ...
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bioRxiv - Bioengineering 2023Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) and index codes were added to attribute sequences to each sample ...
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bioRxiv - Genomics 2023Quote: ... An NGS library was generated using an NEB Ultra II DNA Library Prep kit (NEB, Ipswich, MA) with an Illumina compatible y-adaptor ...