Labshake search
Citations for New England Biolabs :
4801 - 4850 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The metagenomic library was constructed using NEBNext® Ultra DNA Library Prep Kit for Illumina (NEB, USA). The samples were sequenced with the PE150 strategy on Illumina Hiseq Novaseq 6000 platform (commercial service ...
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bioRxiv - Molecular Biology 2023Quote: RT-qPCR experiments were performed with the Luna® Universal One-Step RT-qPCR Kit (NEB, #E3005E) with a final reaction volume of 10 μL per well ...
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bioRxiv - Microbiology 2024Quote: ... inserted in plasmid vector pUC19 with the Gibson Assembly Master Mix kit (New England BioLabs, NEB # E2611S). Mutagenesis was carried out with the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies ...
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bioRxiv - Genomics 2024Quote: ... and TOP2B D64N self-trapping mutants were generated by the Q5 SDM Kit (NEB, catalog no. E0554S) following manufacturer’s instruction and mutation confirmation by sequencing ...
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bioRxiv - Genetics 2024Quote: ... CUT&RUN libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB; E7645L) and sequenced on NextSeq500 (paired-end ...
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bioRxiv - Evolutionary Biology 2024Quote: Libraries were prepared using the NEBNext Ultra II DNA library Kit (New England Biolabs GmbH, Frankfurt, Germany) with purification beads and 100 ng of the fragmented/non-fragmented DNA in a volume of 25 µl were used as input according to the manufacturer’s protocol with some alterations ...
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bioRxiv - Genomics 2024Quote: ... Ribosomal RNA was depleted from total input RNA using the NEBNext rRNA Depletion Kit (NEB cat. #E6310). First and second strand synthesis ...
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bioRxiv - Cell Biology 2024Quote: ... and the vector was extracted from the gel using a Monarch DNA Gel Extraction Kit (NEB #T1020). Gibson Assembly of the three generated fragments was performed using NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621) ...
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bioRxiv - Genomics 2024Quote: ... 200ng of each sample was enzymatically converted using the NEBNext® Enzymatic Methyl-seq Kit (NEB, E7120) with the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Genetics 2024Quote: ... and deletion mutant zebrafish larvae using the Monarch Total RNA Miniprep Kit (New England Biolabs (NEB, T2010S) and reverse-transcribed with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Genetics 2024Quote: ... and deletion mutant zebrafish larvae using the Monarch Total RNA Miniprep Kit (New England Biolabs (NEB, T2010S) and reverse-transcribed with High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Genomics 2024Quote: Final libraries were prepared on beads using the NEBNext Ultra II DNA Library Prep Kit (NEB, #E7645) as follows ...
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bioRxiv - Immunology 2024Quote: ... Complementary deoxyribonucleic acid (cDNA) was synthesized using the ProtoScript First-Strand cDNA Synthesis kit (New England Biolabs). Real-time PCR assays were performed using a CFX Connect Real-Time PCR Detection System (Bio-Rad) ...
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bioRxiv - Physiology 2024Quote: ... Strand-specific libraries were prepared by the NEBNext Ultra II Directional RNA Library Preparation kit (NEB, USA), as described previously (Makhnovskii et al. ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were prepared using NEBNext ultra II DNA library kit (New England Biolabs, Cat. no. E7805S) and unique dual indexes (Integrated DNA Technologies Inc. ...
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bioRxiv - Biochemistry 2024Quote: ... These fragments were cloned into linearized pUC19 using the Hi-Fi Assembly Cloning Kit (New England Biolabs). The tps1Δ mutant was confirmed by PCR and the TPS1IDDΔ strain was confirmed by PCRs and sequencing to confirm the replacement of the CnTps1IDD with the CaTps1IDD ...
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bioRxiv - Biochemistry 2024Quote: ... These fragments were cloned into linearized pUC19 using the Hi-Fi Assembly Cloning Kit (New England Biolabs). The plasmids carrying the desired inserts were transformed into strains by biolistic transformation as previously reported (82) ...
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bioRxiv - Cancer Biology 2024Quote: m6A seq was performed on 2×107 cells using EpiMark N6-methyladenosine enrichment kit (New England Biolabs). The libraries were prepared from purified DNA ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA library preparation was done using the NEBnext Ultra II DNA Library Prep Kit (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA library preparation was done using the NEBnext Ultra II DNA Library Prep Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... end-repair and A-Tailing were directly performed using the NEBNext UltraII DNA kit (New England Biolabs). A new custom universal Y-adapter based on the Illumina TruSeq sequences was designed to include a Unique Molecular Index (UMI ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized from 300 ng of purified RNA using LunaScript RT SuperMix kit (New England BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... POINT-seq libraries were made with the NEBNext® UltraTM II Directional RNA Library Prep Kit (NEB). POINT5-seq libraries were made with the SMARTer Stranded RNA-Seq kit (Takara Bio ...
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bioRxiv - Molecular Biology 2024Quote: ... and in vitro transcribed RNA was purified using the using the Monarch® RNA Cleanup Kit (NEB). Full-length 5’ RACE was performed using the GeneRacer™ with Superscript™ III RT kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... coli isolates was purified using a Monarch DNA purification kit (New England Biolabs Japan Inc., Tokyo, Japan). DNA concentrations were measured using Qubit™ 4 fluorometer with Qubit™ 1× dsDNA High Sensitivity (HS ...
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bioRxiv - Neuroscience 2024Quote: ... RNAseq libraries were constructed using a NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). mRNA pull-down was performed using the Magnetic Isolation Module (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... 250 ng template DNA was supplemented in 10 µl PURExpress in vitro protein synthesis kit mix (NEB). The reaction mix was incubated for 2 h at 30 °C and was subsequently used for assays.
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bioRxiv - Molecular Biology 2024Quote: ... followed by blunting and ligation of the digested plasmid using Quick Blunting and Quick Ligation kits (NEB). For pJLS3-56 ...
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bioRxiv - Molecular Biology 2024Quote: ... and p65AD-HSF1AD using primers 5220/5221 and the Q5® Site-Directed Mutagenesis Kit (NEB, #E0552S). Resulting mutants were confirmed by full plasmid sequencing (Plasmidsaurus).
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bioRxiv - Molecular Biology 2024Quote: ... Long RNAs were in vitro transcribed using the HiScribe T7 High Yield Kit (New England Biolabs SE20405). U4 snRNA was in vitro transcribed using MEGAshortscript T7 Transcription Kit (Invitrogen AM1354) ...
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bioRxiv - Pathology 2024Quote: ... Transcribed crRNA was cleaned up using Monarch@ RNA Cleanup Kit (New England Biolabs, Frankfurt am Main, Germany), crRNA quality and quantity was analysed by gel electrophoresis and Nanodrop ...
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bioRxiv - Molecular Biology 2024Quote: gRNA was generated by in-vitro transcription using the HiScribe™ T7 In Vitro Transcription Kit (NEB) and purified using the RNEasy Mini Kit (Qiagen ...
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bioRxiv - Cancer Biology 2024Quote: ... Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (#7770, New England Biolabs) according to the manufacturer’s protocol and index codes were added to attribute sequences to each sample ...
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bioRxiv - Microbiology 2024Quote: ... The resulting PCR products were checked by gel-electrophoresis and purified (Monarch PCR&DNA Cleanup Kit, NEB) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The point mutants screened for the validation experiment were created via Q5 site-directed mutagenesis kit (NEB) and the pMV306_eccD3 vector ...
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bioRxiv - Microbiology 2024Quote: ... This DNA was cloned in the pET28a(+) vector using the NEBuilder HiFi DNA assembly cloning kit (NEB) and corresponding instructions ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomal RNA was removed using the NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat; New England Biolabs), and the rRNA-depleted RNA was used to prepare libraries with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... The sequencing library was prepared using the NEBNe UltraTM RNA Library Prep Kit for Illumina (NEB, USA). PCR products were purified (AMPure XP system ...
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bioRxiv - Molecular Biology 2024Quote: ... The vector and insert were gel-extracted and purified with a Monarch→ DNA gel extraction kit (NEB) and the fragments were annealed using T4 DNA ligase (NEB ...
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bioRxiv - Microbiology 2024Quote: ... sequencing libraries were generated using the NEBNext Ultra II directional RNA Library Prep Kit for Illumina (NEB) following the manufacturer’s recommendations ...
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bioRxiv - Immunology 2024Quote: ... Polyadenylated transcript enrichment and strand specific library preparation was completed using NEBNext Ultra II mRNA kit (NEB) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... the in vitro transcription was performed using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted using the Monarch® Total RNA Miniprep Kit (New England Biolabs, Frankfurt, Germany). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... The manufacturer’s protocol, “Protocol for FS DNA Library Prep Kit (E7805, E6177) with Inputs ≤100 ng (NEB)” ...
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bioRxiv - Developmental Biology 2024Quote: ... Libraries were cloned using the NEBNext Ultra Directional II RNA Library Prep Kit for Illumina (NEB, E7760), following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were prepared using the NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs). Paired-end sequencing was carried out for 150 cycles using the Nextseq500 system (Illumina Inc. ...
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bioRxiv - Microbiology 2024Quote: ... The plasmid pCgnox2_nat for was assembled using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs) according to the instruction manual ...
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bioRxiv - Genomics 2024Quote: ... Libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) following a published protocol48 ...