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Citations for New England Biolabs :
401 - 450 of 917 citations for Coiled Coil Domain Containing 28B CCDC28B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Beads were resuspended in Fast AP Master Mix (1x Fast AP Buffer containing 80U RNase Inhibitor (M0314, NEB), 2U TURBO DNase (AM2238 ...
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bioRxiv - Immunology 2021Quote: ... and second strand synthesis was carried out by resuspending beads in a master mix containing Klenow Fragment (NEB), dNTPs ...
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bioRxiv - Molecular Biology 2023Quote: ... A mixture containing 1 μl of a 4-fold dilution of Low Range ssRNA Ladder (New England Biolabs) and 0.75 pmol each of RPIX_SC1_Bridge ...
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bioRxiv - Microbiology 2022Quote: ... pRS1 containing the YohP-MS2-6xbs coding sequence was first linearized by EcoRI restriction digest (NEB, Frankfurt, Germany). In vitro transcription was performed using AmpliScribe T7-Flash Transcription kit (Lucigen Corp. ...
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bioRxiv - Molecular Biology 2023Quote: ... MYH7 fragment containing mutation was amplified by PCR using Q5 High-Fidelity DNA Polymerase (New England Biolabs, M0491L) and 500 nM forward and reverse primers (Table 3) ...
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bioRxiv - Biophysics 2023Quote: ... p-SFG retroviral backbone containing an IRES-ΔCD19 reporter gene was linearized by NotI-HF (NEB, ref: R3189S) and XhoI (NEB ...
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bioRxiv - Microbiology 2023Quote: Plasmid DNA containing one copy of the SIV gene was linearized using EcoR1 (New England Biolabs, Ipswich, MA) following their restriction digest protocol (New England BioLabs ...
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bioRxiv - Genomics 2023Quote: ... Approximately 7,500 cells were diluted with 1.25 ml of a dilution buffer containing 0.4x NEBuffer 2 (NEB, B7002S), 2 mg/ml BSA (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... and beads resuspended in a 50-µl PCR mix containing 1x NEBNext High-Fidelity PCR Master Mix (NEB) and 0.5 µM of Nextera Ad1_noMX and Ad2.X primers ...
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bioRxiv - Microbiology 2023Quote: ... samples containing the same amount of protein (20 µg/sample) were processed for endoglycosidase-H (Endo-H, NEB) digestion using 5 milliunits Endo-H for 16h at 37°C ...
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bioRxiv - Physiology 2023Quote: ... The PCR was performed in a total volume of 50 µl containing 1× HF buffer (New England BioLabs), 1 µl dNTP Mix (New England BioLabs) ...
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bioRxiv - Molecular Biology 2023Quote: ... Such probes were generated by linearizing pDONR221-vectors containing the DsuzIR62a-coding sequences using NheI (New England Biolabs) following protocols described above ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... The wash solution was then replaced with 1× T4 polymerase buffer containing 30 U T4 DNA polymerase (NEB) and incubated at 12 °C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... coli in vitro transcription were synthesized through a 200 µL PCR reaction containing 10x Taq Buffer (NEB, #B9014S), 250 µM dNTP Mix (NEB ...
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bioRxiv - Biophysics 2023Quote: A Tandem HaloTag plasmid containing the HaloTag-GTGSGSGS-HaloTag cDNA sequence was built via Gibson Assembly (NEB, (62)) of two HaloTag genes ...
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bioRxiv - Bioengineering 2023Quote: ... cells were resuspended in Rnase H reaction buffer containing 20 U/mL of Rnase H enzyme (NEB M0297S). Cells were incubated for one hour at 37 °C with gentle agitation ...
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bioRxiv - Cell Biology 2024Quote: ... washed and incubated with T cell medium containing 5 μM (Snap-Cell Block S9106S, New England Biolabs, NEB) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... washed and incubated with T cell medium containing 5 μM (Snap-Cell Block S9106S, New England Biolabs, NEB) for 30 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... TAP-PCR was performed in a total volume of 25 μL containing 0.25 μL of Q5 polymerase (NEB), 5 μL of GC Enhancer (NEB) ...
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bioRxiv - Microbiology 2023Quote: NINL (1-702) containing an amino-terminal HaloTag was expressed in BL-21[DE3] cells (New England Biolabs), which were then grown until OD 0.4-0.6 and induced with 0.1 mM IPTG for 16 hr at 16°C ...
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bioRxiv - Biochemistry 2023Quote: ... Oligos containing the spacer sequence and Esp3I sticky ends were annealed and phosphorylated using T4 Polynucleotide Kinase (NEB) and purified using the Oligo Clean and Concentrator kit (Zymo Research) ...
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bioRxiv - Biochemistry 2020Quote: ... Primary antibodies used were: New England Biolabs (NEB), Hitchin ...
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bioRxiv - Molecular Biology 2022Quote: ... and anti-MBP antibody (NEB E8032L; 1:10,000) in 1x TBST supplemented with 5% low-fat milk were incubated either at 4 °C overnight or 1 hr at room temperature ...
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bioRxiv - Immunology 2022Quote: The antibody expression vectors for each recombinant antibody (VH + VL-L/k) were transfected together with a Transposase vector (Hera BioLabs, USA). Cells were selected with Hygromycin B (H3274 ...
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bioRxiv - Plant Biology 2019Quote: Genomic regions containing each of the TIR1/AFB genes were amplified using Phusion polymerase (New England Biolabs or ThermoFisher) from corresponding genomic clones (JAtY51F08 ...
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bioRxiv - Biophysics 2021Quote: ... Each fragment was produced by PCR using primers containing the restriction recognition sites for enzymes BbsI and BsaI (NEB), which recognize asymmetric DNA sequences and cut outside of their recognition sequence ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction was initiated by diluting the LbCas12a complexes to a final concentration of 37 nM LbCas12a : 37 nM sgRNA in a solution containing 1X NEBuffer 2.1 (NEB) and 1 µM custom ssDNA reporter substrates in a 40 μL reaction volume ...
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bioRxiv - Molecular Biology 2020Quote: ... 50 µl of 1xMNase buffer containing 60 gel units/ml (6 Kunz units/ml) of Micrococcal nuclease (NEB M0247S) were added and incubated for exactly for 5 min ...
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bioRxiv - Biophysics 2022Quote: ... The amplified plasmid containing the mutated p66 was double digested with NdeI and XhoI restriction enzymes (New England Biolabs), gel purified and cloned into MCS-2 of the pETDuet-1 vector pre-digested with the same set of restriction enzymes and carrying mutated p51 domain in MCS-1 ...
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bioRxiv - Genomics 2022Quote: ... with a minimal promoter and partial 3’ UTR was amplified from pMPRAduo:minP:GFP (addgene pending) in 1600 μL volume containing 800 μL of Q5 High-Fidelity 2X Master Mix (NEB, M0492L) and 0.5 μM forward and reverse primers (primers 7 and 8 for vector A and primers 9 and 10 for vector P ...
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bioRxiv - Genomics 2020Quote: ... and permeabilized for 5 min on ice in PBS containing 0.5%Triton X-100 and 2 mM Ribonucleoside Vanadyl complex (New England Biolabs). Coverslips were preserved in 70% EtOH at -20°C ...
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bioRxiv - Immunology 2019Quote: ... Single cells were flow-sorted into 96-well plates containing 2 µL of nuclease-free water with 0.2% Triton X-100 and 4 U murine RNase inhibitor (NEB), centrifuged ...
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bioRxiv - Genomics 2020Quote: Cas9 protein and transcribed sgRNA were incubated for 10 min at room temperature in reaction buffer containing 1× NEB buffer 3.1 (NEB Biolabs) supplemented with 1 mM DTT ...
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bioRxiv - Microbiology 2019Quote: ... Amplification was performed in a total volume of 25 µl containing 12.5 µl of 2x OneTaq Mastermix (New England Biolabs, NEB), 9.9 µl of nuclease-free H2O ...
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bioRxiv - Synthetic Biology 2020Quote: ... and subsequently mixed at a 6:1 ratio with the digested backbone in reactions containing T4 DNA ligase (NEB).
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bioRxiv - Genetics 2019Quote: ... the MX-PCR was performed with a total reaction volume of 25 μL containing 12.5 μL Phusion Hot Start Flex 2X Master Mix (New England BioLabs), 25 ng of K562 cell line genomic DNA and 5 μL of the primer pool as defined in Supplementary Table 3 ...
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bioRxiv - Cell Biology 2021Quote: ... clr4 gene specific sgRNA was cloned into Cas9 containing pLSB-KAN plasmid by Golden Gate Assembly kit (E1601S, NEB). The resulting plasmid clr4-pLSB-KAN and clr4 HR template obtained by annealing primer pair WW748-clr4-HR-F/WW749-clr4-HR-R (Table S3 ...
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bioRxiv - Plant Biology 2020Quote: ... A 3 μl aliquot of the purified DNA fragments was blunted in a 20 μl reaction containing 2 μl buffer 2.1 (New England Biolabs), 1 μl 2 mM dNTPs ...
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bioRxiv - Cancer Biology 2020Quote: Genomic DNA was amplified in the region containing the expected KMT5C mutation using Q5 high fidelity polymerase (M0491L, NEB). Primers for amplification and sequencing are outlined in Supplementary Table 3.
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bioRxiv - Biochemistry 2021Quote: ... 2.5 μl of reaction was added to a tube containing 5 μl replication buffer and 1 μl MseI (NEB) for 3 min ...
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bioRxiv - Plant Biology 2020Quote: ... 100ng of each entry and destination vector are assembled in one reaction mix containing 10U BsaI-HF-v2 (NEB), 200U T4 Ligase (NEB) ...
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bioRxiv - Genetics 2020Quote: ... the suspension was then mixed with 200 μl of master mix containing 50 μl 10x T4 ligase buffer (NEB), 2.5 μl 20 mg/ml BSA ...
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bioRxiv - Genetics 2022Quote: ... Eggs were then injected under air-dry conditions with a solution containing 300ng/μl of recombinant Cas9 Nuclease (NEB) and 100ng/ul each of four sgRNAs targeting the first exon of the Oatp74D gene (S2 Table) ...
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bioRxiv - Genetics 2022Quote: ... The swab was then given to the primary investigator and placed in a 1.5 ml microcentrifuge tube containing 400 μl of Cell Lysis Buffer from the Monarch Genomic DNA Purification Kit (NEB). Samples were stored in Cell Lysis Buffer at 4°C until processed.
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bioRxiv - Molecular Biology 2022Quote: ... The pellets containing intact nuclei were vortexed at maximum setting for 15 sec with the nuclear extraction buffer (NEB) and then incubated at 4°C for 30 min with gentle mixing ...
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bioRxiv - Immunology 2022Quote: ... T cell clones were thawed and allowed to rest at 37°C in RPMI containing DNAse (5µg/mL, New England Biolabs).
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bioRxiv - Genomics 2022Quote: ... Cells were fixed with 3% paraformaldehyde in PBS for 10 min at room temperature and permeabilized for 5 min on ice in PBS containing 0.5%Triton X-100 and 2mM Vanadylribonucleoside complex (New England Biolabs). Coverslips were preserved in 70% EtOH at -20°C ...
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bioRxiv - Neuroscience 2020Quote: ... soluble supernatants were immunopreciptated with monoclonal anti-GFP antibody precoupled to Protein G Sepharose beads and immunoprecipitates were washed extensively with IPB containing 0.5M NaCl followed by further washes with IPB and then with lambda phosphatase assay buffer (NEB). Equal volumes of each immunoprecipitated sample were then aliquoted into two tubes and beads were then resuspended with 100 microliters of lambda phosphatase assay buffer containing 400 units of lambda protein phosphatase (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... Beads were then washed once in 5 volumes of binding buffer (100μL, 1xPBS containing 1mg/mL of BSA (NEB)) for 10 minutes at RT ...
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bioRxiv - Microbiology 2020Quote: ... with the exception of a 0.9 kb region containing bla was PCR amplified and joined via Gibson assembly (HiFi DNA Assembly, New England Biolabs) with a 1.3 kb PCR product containing kan PCR amplified from pCM66 [57] to generate pPS04 (Fig S2) ...