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Citations for New England Biolabs :
351 - 400 of 917 citations for Coiled Coil Domain Containing 28B CCDC28B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The ligation mix (20 μl) was added to a 50 μl reaction containing 1X thermopol buffer (NEB), 200 μM each of dATP ...
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bioRxiv - Biochemistry 2022Quote: ... before the unbound fraction containing cleaved Gβ1γ2 was dephosphorylated by treatment with lambda phosphatase (New England Biolabs), calf intestinal phosphatase (New England Biolabs) ...
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bioRxiv - Cell Biology 2023Quote: ... DNA isolated from lysed mouse aortic SMCs was digested with enzyme cocktail containing HindIII – XbaI – EcoRI (NEB). Following digestion ...
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bioRxiv - Genetics 2023Quote: ... The PCR products containing the geneticin128-795-TtrpCand LB border were digested using AgeI (New England Biolabs) followed by a ligation reaction ...
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bioRxiv - Genomics 2023Quote: ... The purified DNA was amplified using a reaction mixture containing 2X NEB PCR Master Mix (NEB M0541L), a universal forward primer i5 ...
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bioRxiv - Cell Biology 2023Quote: ... The integrity of MS2×128-containing lines was further confirmed by PCR using Q5 (New England Biolabs) and Terra (Takara ...
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bioRxiv - Biochemistry 2023Quote: ... Two annealed oligonucleotides containing the homology region were phosphorylated by T4 polynucleotide kinase (New England Biolabs, M201) and cloned into the vectors pX335 and pKN7 (see Appendix Table S1 ...
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bioRxiv - Neuroscience 2023Quote: ... and amplified with primers containing indexes for sequencing using Q5 High-Fidelity 2xMaster mix (New England Biolabs).
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bioRxiv - Cell Biology 2023Quote: ... and iLID constructs were generated by PCR amplifying the parent constructs in pcDNA3.1 (GFP-Gγ3, GFP-Gγ9, and Venus-iLID) with overhangs containing expected nucleotide mutations and DpnI (NEB) digestion (to remove the parent construct ...
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bioRxiv - Biochemistry 2023Quote: BicD2 and NINL containing amino-terminal HaloTags were expressed in BL-21[DE3] cells (New England Biolabs) at an optical density at 600 nm of 0.4–0.6 with 0.1mM IPTG for 16h at 18°C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... containing 12·5 μL of Q5 Hot Start High-Fidelity 2X Master Mix (New England BioLabs, M0494S), 1·1 μL of 10 μM SARS-Cov2-Midnight-1200 primer (either Pool 1 or Pool 2)(17 ...
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bioRxiv - Microbiology 2023Quote: ... pellets dried and resuspended in 100 µL RNase-free water containing 40 U murine RNase inhibitor (NEB). Following quantification by Nanodrop ...
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bioRxiv - Genomics 2023Quote: ... Illumina TruSeq adapters were ligated to DNA fragments in a reaction containing 2X Quick ligation buffer (NEB), 0.25 μM of adapter and 2 μL of Quick ligase (NEB ...
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bioRxiv - Systems Biology 2023Quote: ... 22 μL of a mixture containing 10 mM of each of the four deoxynucleotide triphosphates (dNTPs) (NEB), 5.5 μL of TSO primer (100 μM) ...
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bioRxiv - Biophysics 2024Quote: ... solutions containing polySH3 and cleaved MBP- and His6-tags were passed over Amylose resin (New England Biolabs) to remove excess MBP ...
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bioRxiv - Cancer Biology 2021Quote: ... Bound antibodies were detected by incubation with anti-rabbit or anti-mouse DyLight 800-conjugated secondary antibodies (New England BioLabs). Slide images were acquired using an InnoScan 710-IR scanner (Innopsys ...
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bioRxiv - Microbiology 2020Quote: ... All subsequent HI.fate vectors containing different internal promoters were constructed by digesting HI.fate.E with BsiW I and Sac II restriction enzymes (NEB) and cloning each promoter ...
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bioRxiv - Cell Biology 2019Quote: ... We incubated our cells in complete medium containing 2 μM of SNAP-Cell TMR-Star (New England Biolabs) during 20 min to label all pre-existing available SNAP-tag (Pulse) ...
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bioRxiv - Molecular Biology 2019Quote: ... and a DNA fragment containing GFP were inserted into pCAGEN by NEBuilder HiFi DNA Assembly Master Mix (NEB).
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bioRxiv - Evolutionary Biology 2022Quote: ... This was then loaded and incubated in a gravity column containing 25 mL of chitin bead slurry (NEB), then washed with 400 mL column buffer (20 mM TrisHCl pH 8 ...
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bioRxiv - Cell Biology 2019Quote: ... the cells were stained with DMEM containing 1 µM SNAP-Cell SiR (New England Biolabs, Ipswich, MA, USA) for about 10 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... the 5′ linker (containing four random nucleotides) was ligated to the small RNAs using T4 RNA ligase (NEB) overnight at 16°C ...
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bioRxiv - Immunology 2019Quote: ... and second strand synthesis was carried out by resuspending beads in a master mix containing Klenow Fragment (NEB), dNTPs ...
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bioRxiv - Biochemistry 2021Quote: ... primers containing overhangs of the 3x FLAG peptide were phosphorylated using the T4 polynucleotide kinase (New England BioLabs). Phosphorylated primers were used to PCR amplify pQLinkN-pgaD to generate the plasmid pQLinkN-pgaD-FLAG ...
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bioRxiv - Immunology 2021Quote: ... the samples were added to 2 μL of second-strand synthesis mix containing 2.25× NEB buffer 2 (NEB), 0.625mM each dNTP Mixture (NEB) ...
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bioRxiv - Systems Biology 2020Quote: ... and second strand synthesis was carried out by resuspending beads in a master mix containing Klenow Fragment (NEB), dNTPs ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA-bound beads were resuspended in 100µl of mix containing 82µl of 1X NEB T4 DNA ligase buffer with 10mM ATP (NEB), 10µl of 10 (2.5mM each ...
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bioRxiv - Genetics 2021Quote: ... Different SCON variant fragments containing the respective complementary ends were then inserted into eGFP with SapI (R0569S, NEB) and T4 ligase for 20 cycles of 2 minutes at 37 °C and 5 minutes at 16 °C ...
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bioRxiv - Biochemistry 2022Quote: AP-DNA was prepared by incubating 1 μM uracil-containing oligonucleotide with 0.6 U UDG (New England Biolabs) (55 ...
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bioRxiv - Microbiology 2022Quote: ... samples containing the same amount of protein (50 µg/sample) were processed for endoglycosidase-H (Endo-H, NEB) digestion using 5 milliunits Endo-H for 16h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... The cDNA was used as template in PCR reactions containing the Taq 2X Master Mix (New England Biolabs) and 5 μM of MP2- and scorpine-specific primers (Table S7) ...
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bioRxiv - Immunology 2022Quote: Plasmids containing templates for CRISPR-mediated KI were assembled using the NEBuilder HIFI assembly kit (New England Biolabs) starting from PCR amplified fragments ...
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bioRxiv - Molecular Biology 2019Quote: ... Non-overlapping forward and reverse primers containing the desired mutation were phosphorylated by polynucleotide kinase (New England Biolabs) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... cells were collected and incubated in fresh SM containing 10 µM SNAP-Cell Block (New England Biolabs S9106), for 30 min at 25°C ...
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bioRxiv - Cell Biology 2019Quote: ... 50mM Tris (pH 8.5-8.8)) containing protease and phosphatase inhibitors (cOmplete Mini, PhosSTOP, Roche; 2mM Sodium Orthovanadate, NEB), sonicated to shear chromatin and centrifuged to remove cellular debris ...
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bioRxiv - Molecular Biology 2020Quote: ... EcoRV de-staining buffer (deionized water containing 1X cut-smart buffer and 200 units of EcoRV (NEB, #R3195S) in 500ul) ...
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bioRxiv - Molecular Biology 2020Quote: ... DNase I de-staining buffer (PBS containing 1X DNase buffer and 20 units of DNase I (NEB, #M0303) in 500ul) ...
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bioRxiv - Microbiology 2021Quote: ... Transposon-containing fragments were enriched by PCR using ComP7 primers ComP5 using Phusion DNA polymerase (New England Biolabs) in a 20-cycle reaction ...
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bioRxiv - Microbiology 2021Quote: ... The PCR for each sample was performed in 50µl total volume: containing 0.5µl Q5 High-Fidelity DNA polymerase (New England Biolabs, USA), 10µl 5X Q5 buffer ...
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bioRxiv - Biochemistry 2019Quote: ... streptavidin tips were incubated overnight in 50 mM ammonium bicarbonate containing 1,000 units PNGase F (New England Biolabs) at 37 °C ...
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bioRxiv - Genomics 2021Quote: ... 1uL of the oligo mix was added to a master mix containing 1x T4 ligase buffer (NEB M0202M), 0.5 uL T4 PNK (NEB M0201L ...
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bioRxiv - Genetics 2021Quote: ... We then created a construct containing the alternate allele using the NEB Q5 SDM kit (New England Biolabs). The primer sequences used were:
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bioRxiv - Biochemistry 2021Quote: ... His tagged proteins were eluted with lysis buffer containing 400mM imidazole and bound immediately to amylose resin (NEB) for 1hr ...
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bioRxiv - Biochemistry 2021Quote: ... fully assembled complexes containing His-Rpn12 and MBP-Rpn6 were purified using a HisTrap and amylose resin (NEB), and cleaved with HRV-protease ...
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bioRxiv - Microbiology 2021Quote: ... Standard curve was prepared using SARS-CoV-2 Positive Control plasmid containing full nucleocapsid protein (N gene) (NEB) and used to quantify copies of N gene in organoid samples ...
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bioRxiv - Biochemistry 2021Quote: ... 50 μl of lysed cells were aliquoted to 8 tubes containing 450 μl of digestion mix (1× NEB 1 buffer ...
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bioRxiv - Cell Biology 2020Quote: 50 μl of lysed cells were aliquoted to 8 tubes containing 450 μl of digestion mix (1x NEB 1 buffer ...
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bioRxiv - Genomics 2021Quote: ... in a 25 μL reaction containing 1X PNK buffer and 15 units of polynucleotide kinase (New England Biolabs) by incubating at 37°C for 45 minutes ...
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bioRxiv - Genomics 2020Quote: ... A construct containing the alternate allele was then created using the NEB Q5 SDM kit (New England Biolabs). The primer sequences used were as follows:
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bioRxiv - Immunology 2020Quote: ... before amplification with Illumina adapter-containing primers (Nextera i7 indices) and NEBNext Ultra II Q5 Master Mix (NEB) as follows ...