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Citations for New England Biolabs :
451 - 500 of 917 citations for Coiled Coil Domain Containing 28B CCDC28B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Annealed adapters were ligated onto DNA by resuspending the beads in a 100 μL reaction containing Quick Ligase (NEB) and 0.1 μM annealed i5/i7 adapter ...
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bioRxiv - Microbiology 2021Quote: ... or RCC970 in 50 μl total volume containing 5 μl 10x Q5 Reaction Buffer (NEB, Frankfurt am Main, Germany), 0.5 U of Q5 High-Fidelity DNA Polymerase (NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... so the bead-bound proteins were dephosphorylated in wash buffer containing 1:50 Lambda Protein Phosphatase (New England Biolabs) and 1 mM MnCl2 to make the phosphosites more accessible for a kinase assay ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The ITS PCR reactions were performed in 25 μl with the following composition: 5 μL 5× buffer containing MgCl2 at 1.5 mM (New England Biolabs), 0.1 mM each dNTP ...
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bioRxiv - Cell Biology 2022Quote: ... 100ng of each entry and destination vector are assembled in one reaction mix containing 10U BsaI-HF-v2 (NEB), 200U T4 Ligase (NEB) ...
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bioRxiv - Cell Biology 2019Quote: ... cells were collected and incubated in fresh medium containing 4 μM SNAP-Cell TMR-Star (New England Biolabs, Inc.) for 15 min at 25°C ...
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bioRxiv - Microbiology 2019Quote: ... Ten ng DNA isolated from vegetative cells grown in AA/8 and AA/8N and 10 ng DNA isolated from heterocysts were added to a 20-μL reaction containing 0.2 units of Phusion High-Fidelity DNA Polymerase (NEB), 1X Phusion buffer ...
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bioRxiv - Microbiology 2020Quote: ... Template DNA was amplified in duplicate 20 μl reactions containing 1.0 U Q5 high fidelity DNA polymerase (NEB, UK), 0.25 μM primers ...
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bioRxiv - Molecular Biology 2020Quote: ... SmaI de-staining buffer (de-ionized water containing 1X cut smart buffer and 200 units of SmaI (NEB, #R01041S) in 500ul).
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bioRxiv - Molecular Biology 2020Quote: ... in a total volume of 20 µL containing 10 U of T4 RNA Ligase 1 (NEB, cat n° M0204L), 1X T4 RNA ligase buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... the plug was incubated in 160 μl of 1× NEBuffer 3.1 buffer containing 160 units of Bgl II (New England BIolabs) overnight at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... the nuclei were pelleted and supernatants were transferred to 2.0 ml screw-top tubes with O-rings containing magnetic beads (SureBeads Protein G Magnetic Beads, NEB) coupled to antibodies (anti-pan Ago antibody ...
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bioRxiv - Genetics 2020Quote: ... and resuspended in 750 µl ligation mixture containing 200 units T4 DNA ligase (NEB/ IMB core facility protein production), 1X T4-Ligation buffer (NEB ...
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bioRxiv - Genomics 2021Quote: ... and permeabilized for 5 min on ice in PBS containing 0.5% Triton X-100 and 2 mM Ribonucleoside Vanadyl complex (New England Biolabs). Coverslips were preserved in 70% EtOH at −20 °C ...
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bioRxiv - Genetics 2021Quote: ... Cells were fixed in 3% paraformaldehyde in PBS for 10 min at room temperature and permeabilized for 5 min on ice in PBS containing 0.5%Triton X-100 and 2 mM Vanadyl-ribonucleoside complex (New England Biolabs). Coverslips were stored in −20°C in 70% EtOH until further use.
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bioRxiv - Genetics 2020Quote: ... A 6 µL mixture containing 2 µL of 40 µM Spy Cas9 NLS protein (New England Biolabs, MA, USA), 200 ng each of five sgRNAs (in 2 µL ...
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bioRxiv - Evolutionary Biology 2020Quote: ... From the resulting plasmid a fragment containing CRE-bGH-poly(A) was excised with restriction enzymes SalI (NEB, R3138S) and AleI (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... 0.5 μg of 32P-labelled R-loop-containing plasmid was either mock-treated or incubated with 0.5 U RNase H (NEB) in 75 mM KCl / 50 mM Tris-HCl pH 7.5 / 3 mM MgCl2 / 10 mM DTT in a 20 μL reaction volume for 30 minutes at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... Lysates containing 6xHis-MBP-OmpW and 6xHis-MBP-EF-Tu were incubated on amylose resin (New England Biolabs, E8021L) (Qiagen ...
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bioRxiv - Genomics 2020Quote: ... followed by addition of 20 μl Ligation master mix (containing: 15 μl NEB Quick ligation buffer, 0.4 μl NEB Quick Ligase ...
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bioRxiv - Molecular Biology 2021Quote: ... sequence in a reaction containing 40 ng/μl DNA (4 μg total) and 0.5 U/μl restriction enzyme (HindIII-HF; NEB, Cat ...
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bioRxiv - Microbiology 2020Quote: ... regions of ~600 bp containing the sgRNA sequence were amplified using NEBNext Ultra II Q5 Master Mix (NEB #M0544S) in 25 amplification cycles ...
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bioRxiv - Immunology 2020Quote: ... annealed oligos containing the sgRNA targeting sequence were phosphorylated and cloned into a dephosphorylated and BsmBI (New England Biolabs) digested SgOpti (Addgene#85681 ...
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bioRxiv - Molecular Biology 2021Quote: ... Each amplification template containing appropriate copy number of the gene was added into the master mix containing 1 μL of Bst 3.0 DNA polymerase (Cat. No. M0374L, NEB), 2.5 μL of 10 × isothermal amplification buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... the first step PCR reaction was performed in a 12.5 μl mixture containing 1X OneTaq Hot Start Master Mix (NEB), 0.4 μM of each DHPV-U 1538F and DHPV-U 1887R primer and 20 ng of DNA template ...
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bioRxiv - Microbiology 2021Quote: ... containing mRNA was isolated from 1μg total RNA using NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). cDNA libraries were constructed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina and NEBNext Multiplex Oligos for Illumina ...
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bioRxiv - Immunology 2020Quote: ... annealed oligos containing the sgRNA targeting sequence were phosphorylated and cloned into a dephosphorylated and BsmBI (New England Biolabs) digested SgOpti (Addgene#85681 ...
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bioRxiv - Biochemistry 2022Quote: ... The beads were covered with 30 μL of reverse transcription mixture containing dNTPs (0.5 mM each, New England BioLabs), acetylated BSA (1,5 μg) ...
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bioRxiv - Genetics 2022Quote: ... Embryos were fixed in 3% paraformaldehyde in PBS for 10 min at room temperature and permeabilized for 4 min on ice in PBS containing 0.5% Triton X-100 and 2 mM Vanadyl-ribonucleoside complex (New England Biolabs). Coverslips were stored in 70% EtOH in −20°C no longer than 1 day before further processing.
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bioRxiv - Molecular Biology 2023Quote: ... ddH2O and 2μL of diluted cDNA were used in 10μL qPCR reactions containing the Luna® Universal qPCR Master Mix (NEB) and 0.3μM of gene-specific forward and reverse primer ...
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bioRxiv - Genomics 2022Quote: ... and inserting a 1969bp custom gBlock (IDT) containing EcoRI-myrGFP-StuI-Dam-MCS-Xbal via NEB HiFi assembly (NEB). The myrGFP sequence represents the first 85aa of D ...
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bioRxiv - Plant Biology 2022Quote: ... we first created a RUBY-minus vector lacking the gene GT by assembling PCR product 1 containing CYP76AD1 and DODA and PCR product 2 containing Arabidopsis HSP18.2 terminator into a pGFPGUSplus vector 18 via NEBuilder HiFi DNA Assembly (New England BioLabs). The pAXY0006 vector of split-RUBY was generated by assembling PCR products containing f1 fragment of gene GT (named GTf1 ...
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bioRxiv - Molecular Biology 2023Quote: ... 8 μl of a mix preheated to 37°C and containing 2.5 U of RNase H (New England Biolabs), 2.5× RNase H reaction buffer (1× buffer ...
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bioRxiv - Cancer Biology 2023Quote: Plasmids containing the “circularization cassettes” were generated by using NEBuilder HiFi DNA Assembly Cloning Kit (#E5520S; New England BioLabs) and KLD Enzyme Mix (#M0554S ...
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bioRxiv - Immunology 2023Quote: ... Amplicon containing U6 promoter and sgRNA4 was PCR amplified using Phusion High-Fidelity DNA Polymerase (New England Biolabs, M0530) and assembled into SalI linearized LentiGuide-GBP-Chr3 sgRNA3 plasmid using HiFi DNA Assembly Kit (New England Biolabs ...
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bioRxiv - Immunology 2023Quote: ... were single-cell sorted into 5 µl 1% (v/v) Nonidet P40 Substitute, Tris-HCl (20 mM, pH 8.0) containing 5 U murine RNase inhibitor (NEB) using a FACSAriaIII instrument (BD) ...
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bioRxiv - Bioengineering 2023Quote: ... reactions were carried out in a total of 25 μL containing 1 X Isothermal Amplification Buffer (New England Biolabs), 5 mM MgSO4 ...
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bioRxiv - Genomics 2023Quote: ... nuclei were sorted into a tube of a PCR tube strip containing 5 μL 1X CutSmart buffer (NEB, B7204S) per well ...
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bioRxiv - Bioengineering 2023Quote: ... previously described 34,51 containing the tetracycline resistance cassette and sacB3 gene via a three-fragment HiFi reaction (New England Biolabs). Escherichia coli S17-1 was transformed with the pMTL70641:ΔpilA construct and conjugated with C ...
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bioRxiv - Plant Biology 2023Quote: ... Mutations (D to V) of the MHD motif were carried out using inverse PCR with primers containing AarI (NEB) or BsmBI (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... DNA ligation was performed in the same reaction mixture containing the phosphorylated samples using 5U T4 DNA ligase (NEB). A transformation assay was performed using 3 μL DNA ligation product in DH5alpha bacterial cells and the positive clones were verified by Sanger sequencing.
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bioRxiv - Neuroscience 2023Quote: ... sense and antisense oligos containing the 20 bp guide target sequence wereannealed and phosphorylated with T4 Polynucleotide Kinase (NEB), then inserted between BbsI sites in the plasmid ...
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bioRxiv - Developmental Biology 2023Quote: ... The next day samples were washed twice with dig-wash (wash buffer with 0.025% digitonin) and then resuspended in 150 µl dig-wash containing pA-MNase (New England Biolabs 700 ng/ml ...
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bioRxiv - Biochemistry 2023Quote: ... were generated using the vPRC1.4 recombined plasmid (containing all three expression cassettes) as template and using Phusion polymerase (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: Mutant variants of the mS29-containing construct were generated using the Q5 site-directed mutagenesis kit (New England Biolabs). Mutagenesis primers were designed using the NEBaseChanger tool ...
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bioRxiv - Biochemistry 2023Quote: 3 μL of the transcription and co-transcriptional reactions were added to a 6 μL mixture containing 1x DNase I reaction buffer and 0.5 U/μL DNase I (New England Biolabs). After incubation at 37°C for 30 min ...
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bioRxiv - Microbiology 2023Quote: A 10 µL reaction containing 200 ng of pTrc200HA plasmid DNA and 1× CutSmart Buffer (New England BioLabs, USA) with partially purified V2c or its variants normalized by the major V2c protein band was incubated at 37°C for 1 hour ...
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bioRxiv - Biophysics 2023Quote: ... The fractions containing CrtSPARTA protein complex was pooled and further loaded onto an amylose resin column (New England Biolabs). The column was washed with 20 column volumes (CV ...
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bioRxiv - Genetics 2024Quote: ... in reactions containing 1X ThermoPol buffer (12.5mM tricine, 42.5mM KCl, 1.5mM MgCl2, 6% dextran; New England BioLabs, Ipswich, MA), 0.32 mM dNTP mix ...
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bioRxiv - Genomics 2024Quote: ... 500 µl lysed cells were then transferred to a tube containing 4.5 ml digestion buffer (1% Triton X-100, 1x CutSmart buffer (New England Biolabs)) ...