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Citations for New England Biolabs :
4151 - 4200 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Specific gRNA and KOD PCR were generated using the Q5 site-directed mutagenesis kit (New England Biolabs) on the pSAG1::Cas9-U6::sgUPRT vector (42 ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) with 9 cycles PCR amplification ...
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bioRxiv - Molecular Biology 2021Quote: Samples with CT <25 were submitted to cDNA synthesis protocol using LunaScript™ RT SuperMix Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: RNA-sequencing libraries were produced with NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) from hiPSC-derived astrocytes of ST ...
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bioRxiv - Genomics 2021Quote: ... In Vitro Transcription (IVT) was performed with T7 high yield RNA synthesis kit (New England Biolabs, E2040S). The RNA from the IVT was purified using TRIzol first (Ambion ...
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bioRxiv - Developmental Biology 2021Quote: ... Comparable total RNA quantities were used for reverse transcription with LunaScript RT SuperMix Kit (New England Biolabs). cDNA was amplified with Phusion polymerase for 30-36 cycles and resulting PCR products were separated on a standard 1% agarose gel next to a 100 bp ladder (NEB) ...
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bioRxiv - Cell Biology 2020Quote: DNA libraries were generated using the NEBNext DNA Library Preparation Kit Ultra II (New England Biolabs, USA). Libraries were generated using 15ul of the ccfNA according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2020Quote: ... Site-directed mutagenesis was performed using Q5 Hot Start High Fidelity Kit from New England Biolabs (NEB). Vectors were transformed into E ...
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bioRxiv - Biochemistry 2021Quote: ... All Pf-SUMO and Pf-Ubc9 mutants were generated using Q5 Site-Directed Mutagenesis kit (NEB #E0554S) in Pf-SUMO wild type backbone ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... cDNA libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) and cDNA fragments (150 ∼ 200 bp in length ...
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bioRxiv - Biochemistry 2021Quote: ... Mutations were introduced into this construct using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs), and proteins were expressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Biochemistry 2021Quote: ... The PCR fragment and the plasmid (pOPINS3C) were assembled using the NEBuilder HiFi DNA Assembly kit (NEB), following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina® (New England Biolabs) was used to generate the RNA libraries ...
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bioRxiv - Molecular Biology 2021Quote: ... Ribosomal RNA was depleted from 300 ng total RNA using the NEBNext rRNA Depletion Kit (NEB, #E6310L), and libraries were generated for next-generation sequencing using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA was synthesized for qPCR quantification using the LunaScript RT SuperMix Kit (New England Biolabs; Massachusetts, USA). qPCR reactions were composed of 7.5 μL Power SYBR Green PCR Master Mix (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... was obtained from Origene and mutations were inserted by site-directed mutagenesis using the Q5 kit (E0554S; New England Biolabs NEB) according to the manufacturer’s instructions and using primers in the table below ...
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bioRxiv - Immunology 2021Quote: ... was obtained from Origene and mutations were inserted by site-directed mutagenesis using the Q5 kit (E0554S; New England Biolabs NEB) according to the manufacturer’s instructions and using primers in the table below ...
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bioRxiv - Cancer Biology 2020Quote: ... 1ng of ChIP DNA was taken to prepare library using NEBNext kit for Illumina (New Engand Biolabs) following manufacturer’s instructions and the quality or fragment size was assessed using the Bioanalyser High Sensitivity DNA kit (Agilent) ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: 5ng of purified ChIP DNA was used for library preparation using NEB ultra II DNA kit (NEB) as described in the earlier section ...
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bioRxiv - Cancer Biology 2021Quote: ... and a first strand library prepared using NEBNext Ultra Directional RNA Library Prep Kit (New England Biolabs) and sequenced on a HiSeq 2500 (Illumina).
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bioRxiv - Cancer Biology 2021Quote: Libraries were prepared using the NEBNext Ultra 2 DNA Library Preparation Kit (New England Biolabs, MA, USA) with AMPure® XP Beads (Beckman Coulter ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... These deletions were carried out with the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA). All proteins were overexpressed in E ...
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bioRxiv - Developmental Biology 2021Quote: ... and libraries were prepared using the NEBNext Ultra RNA Library Prep kit for Illumina (New England Biolabs). Libraries were subjected to high-throughput sequencing on an Illumina HiSeq 2500 system (150 cycles ...
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bioRxiv - Biophysics 2020Quote: sgRNAs were produced by in vitro transcription using the HiScribe T7 High Yield RNA synthesis kit (NEB) with PCR amplified gBlocks (IDT ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were generated using NEBNext® Ultra(tm) DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
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bioRxiv - Cell Biology 2020Quote: ... which was inserted into the L4440 vector backbone using the NEBuilder HiFi DNA assembly cloning kit (NEB).
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bioRxiv - Cell Biology 2020Quote: ... mRNA enrichment was achieved from 400-900ng of total RNA using a Magnetic mRNA Isolation Kit (NEB). Generation of double stranded cDNA and library construction were performed using NEBNext® mRNA Sample Prep Reagent Set 1 (NEB) ...
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bioRxiv - Genetics 2021Quote: ... The kinase-dead MELKT167A mutant was constructed using a Q5 site-directed mutagenesis kit (New England Biolabs) with 5′-aggattaccatctacaggcatgctgtgggagtctg-3′ forward and 5′-cagactcccacagcatgcctgtagatggtaatcct-3′ reverse primers.
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bioRxiv - Genetics 2021Quote: ... the fragments were analyzed by gel electrophoresis and purified using a Monarch DNA Gel Extraction Kit (NEB). All strains had the expected variants in the sequenced fragment ...
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bioRxiv - Genomics 2020Quote: ... We extracted RNA using Monarch® Total RNA Miniprep Kit (New England BioLabs GmbH, Frankfurt, D, E2010). Material was ground in Mixer Mill 200 (Retsch GmbH ...
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bioRxiv - Genomics 2020Quote: ... we converted purified mRNA to 300 bp fragment DNA libraries with the Ultra II Directional kit (NEB) and indexed them for multiplexing (NEB).
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: Total RNA was isolated from cells with the Monarch Total RNA Miniprep Kit (New England BioLabs, #T2010S) according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: ... This plasmid was then digested with BamHI and NotI and the resulting insert (5’esaAflank_SpecPromoter_kanR_3’esaAflank) was gel extracted (Monarch DNA Gel Extraction Kit, NEB). 100 ng of purified insert was transformed into S ...
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bioRxiv - Microbiology 2020Quote: ... total isolated RNA was reverse transcribed using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB), according to the manufacturer’s instructions and using a random primer mix (Promega) ...
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bioRxiv - Plant Biology 2021Quote: ... The complementary DNA (cDNA) was synthesized using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs) following the manufacturer’s instructions with primer d(T)23 VN ...
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bioRxiv - Microbiology 2021Quote: ... or built directly into the plasmid of interest using the NEBuilder HiFi Assembly kit (New England Biolabs), transformed into E ...
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bioRxiv - Microbiology 2021Quote: ... crRNA was synthesized in vitro using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) by incubating the reaction at 37°C with T7 promoter primer (10μM ...
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bioRxiv - Molecular Biology 2021Quote: ... all ChIP-DNA libraries were produced using the NEBNext Ultra II DNA Library Prep Kit (NEB, E7645L) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... and in vitro synthesized RNA was subsequently purified using Monarch RNA Cleanup Kit (Cat. No. T2040S, NEB). RNA concentration was quantified by NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... followed by library preparation with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Library preparation included 11 cycles of PCR amplification ...
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bioRxiv - Microbiology 2021Quote: ... SERBP1 mutant and Vigilin mutant were obtained using the Q5® Site-Directed Mutagenesis Kit (E0554) (NEB) with deletion primers using the WT cDNA in pLVX as template ...
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bioRxiv - Immunology 2020Quote: ... (2) and (3) was synthesized through HiScribe™ T7 ARCA mRNA Kit (with tailing) (New England Biolabs). All the mRNA products were concentrated and purified via EZ-10 Spin Column RNA Cleanup & Concentration Kit (Bio Basic Inc.) ...
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bioRxiv - Microbiology 2021Quote: ... were Deletions and point mutations in HelR were created using the Q5 site directed mutagenesis kit (NEB). Primers for mutant construction were designed using the NEBaseChanger as recommended ...
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bioRxiv - Microbiology 2021Quote: ... The DNA samples were pooled into two libraries prepared with the NEBNext Ultra kit (New England BioLabs). They were sequenced on a MiSeq sequencer (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... DNA fragments were amplified by PCR and assembled by NEBuilder HiFi DNA Assembly Cloning Kit (NEB, E5520S).
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bioRxiv - Genetics 2020Quote: ... RNA was reverse transcribed to cDNA using the ProtoScript II First Strand synthesis kit (NEB, Cat# E6560S) and synthesis was performed with the “random primer” option included within the kit ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Genomics 2021Quote: ... we purified the genomic DNA from each timepoint with the Monarch gDNA extraction Kit (New England Biolabs). We then amplified the region of one of the mutations acquired by the Tf-null strain (I:1453108 C->A ...
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bioRxiv - Genomics 2021Quote: ... gDNA was extracted with the Monarch Genomic DNA purification kit (#T3010 New England Biolabs; Ipswich, MA, USA). For Oxford Nanopore sequencing of STK005 ...
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries were produced using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB). Libraries were sequenced on Illumina HiSeq platform.