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Citations for New England Biolabs :
3951 - 4000 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: DNA was extracted from HLCs using the Monarch® Genomic DNA Purification Kit (New England BioLabs, T3010). A master mix was prepared using the Luna® Universal qPCR Master Mix (New England BioLabs ...
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bioRxiv - Molecular Biology 2021Quote: ... and final RNA-seq libraries generated using the NEBNext Ultra II Directional RNA Library Prep kit (NEB, E7760 and NEBNext Multiplex Oligos (NEB ...
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bioRxiv - Genomics 2019Quote: ... Fragmented DNA was used for library preparation using a NEBNext Ultra II Kit (NEB, Ipswich, MA USA) according to the manufacturer’s instructions for DNA end repair ...
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bioRxiv - Genetics 2021Quote: ... cDNA library was prepared with NEBNext Ultra II RNA library prep kit for Illumina (New England Biolabs), and the sequencing carried out using Illumina’s HiSeq-2500 sequencer with single-end mode and read length of 50 bp ...
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bioRxiv - Genetics 2020Quote: ... RNA was then reverse transcribed to cDNA using LunaScript RT SuperMix kit (Catalog # E3010L, New England BioLabs). w1118 flies might have partial or truncated transcripts of w+ ...
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bioRxiv - Genetics 2020Quote: ... Variants were generated using three-way Gibson cloning using NEB HiFi DNA Assembly Cloning Kit (NEB, E5520), using a NotI-AscI-digested and purified pENTR backbone (Thermo Scientific ...
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bioRxiv - Plant Biology 2019Quote: ... Libraries were prepared from the resulting mRNA with the NEBNext® Ultra™ II kit (NEB #E7770S). sRNA seq libraries were generated with the NEBNext® Multiplex Small RNA kit (NEB #E7300S) ...
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bioRxiv - Cancer Biology 2021Quote: ... following the manufacturer’s protocol A160141 v01 for library amplification using the NEBNext Ultra II kit (NEB E7645S).
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bioRxiv - Cell Biology 2020Quote: ... each sample received a different barcoded adapter included in the NEBNext Multiplex Oligos kit (New England BioLabs) to allow for multiplexed sequencing ...
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bioRxiv - Immunology 2021Quote: ... The adaptor-ligated DNA fragments were indexed with the NEBNext Multiplex Oligos for Illumina kit (NEB, E7335). Adapted and indexed libraries were quantified using a KAPA Library Quantification Kit (Roche ...
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bioRxiv - Immunology 2021Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Genomics 2020Quote: ... T7 transcription templates were transcribed using HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs). Reactions were cleaned up using the MEGAClear Transcription Clean-up Kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Products of PCR amplification were subjected to KLD reaction using Q5 Site-Directed Mutagenesis Kit (NEB, USA) and transformed into NEB 5-alpha Competent E ...
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bioRxiv - Microbiology 2021Quote: ... Truncated versions of this minigenome were generated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) using the 3L5LEfull-Luc as a template.
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bioRxiv - Genomics 2021Quote: One reaction of NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645) was used for 1 μg of input DNA ...
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bioRxiv - Systems Biology 2020Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... Transcription of the RNA was carried out using a Highscribe T7 High-yield RNA synthesis kit (NEB), after linearisation of the DNA plasmid using HindIII ...
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bioRxiv - Cell Biology 2020Quote: ... These peptides were used as the substrate in a PKA kinase assay kit (New England Biolabs, #P6000S) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... two oligos (see Supplementary Table 3) were annealed and 5’ phosphorylated (T4 Polynucleotide Kinase kit, M0201S, NEB) as described previously (LentiGuide-Puro and LentiCRISPRv2) ...
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bioRxiv - Neuroscience 2020Quote: ... In vitro transcription of sgRNA template was performed using HiScribe Quick T7 kit (New England Biolabs #E2050S) following the manufacturer’s directions and incubating for 4 hr at 37°C ...
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bioRxiv - Bioengineering 2020Quote: Total RNA was extracted from 1 million cells using the Monarch Total RNA Miniprep Kit (NEB, #T2010S). We prepared cDNA from 1 μg of extracted RNA using LunaScript® RT SuperMix Kit (NEB ...
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bioRxiv - Bioengineering 2020Quote: ... We prepared cDNA from 1 μg of extracted RNA using LunaScript® RT SuperMix Kit (NEB, #E3010). No Template and No Reverse Transcriptase controls (NTC and NRT ...
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bioRxiv - Bioengineering 2020Quote: Genomic DNA was prepared from 1 million cells using the Monarch Genomic DNA Purification Kit (NEB, #T3010G). Diagnostic PCR was then carried out followed by gel extraction (NucleoSpin ...
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bioRxiv - Bioengineering 2021Quote: ... were PCR-amplified and cloned into the pX601 plasmid using an NEBuilder HiFi DNA assembly kit (NEB).
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bioRxiv - Bioengineering 2021Quote: ... the PCR reaction was purified via Monarch® DNA Gel Extraction Kit (New England Biolabs® Inc.). Plasmids were constructed via the Hot Fusion Assembly Method (Fu et al. ...
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bioRxiv - Bioengineering 2021Quote: ... palustris gDNA which was obtained using Monarch® Genomic DNA Purification Kit (New England Biolabs® Inc.). A gentamicin selection marker was included in the expression cassette to force segregation into the endogenous plasmid ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Libraries for sequencing were prepared with the NEBNext Ultra II RNA Prep Kit (New England BioLabs, Inc.) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... Libraries were prepared using NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs E7770S) and the NEBNext Poly(A ...
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bioRxiv - Biochemistry 2020Quote: ... The Q5 site-directed mutagenesis kit was used in all site-directed mutagenesis experiments (New England Biolabs). The HRG-1 coding region was amplified from cDNA prepared from C ...
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bioRxiv - Biochemistry 2021Quote: ... R77E/A variants were produced by site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs), verified by sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... Libraries were barcoded using NEBnext Ultra DNA Library Prep Kit Master for Illumina (New England Biolabs, E7370S) and NEBNext Multiplex Oligos for Illumina (New England Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... the library for sequencing was generated using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) following the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were prepared using NEBNext Ultra II non-directional RNA Library Prep kit (New England BioLabs, # E7770S). Library quality and concentration was assessed with Labchip and qPCR ...
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bioRxiv - Cell Biology 2021Quote: The NGLY1A1201T mutant was generated using the Q5 Site-Directed Mutagenesis Kit (Cat# E0552S, New England Biolabs) using the pLenti-CMV-Puro-NGLY1 construct ...
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bioRxiv - Bioengineering 2020Quote: ... RNA-seq libraries were generated using NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs) following manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... Reactions of 100 µL volume were prepared using the PURExpress Δ Ribosome Kit (New England Biolabs, E3313S) containing 0.8 U/µL of SuperASE-In ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids harbouring MICAL1 domain mutants were generated using Q5® Site-Directed Mutagenesis Kits (New England BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... ChIP-seq libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit (New England BioLabs). Total RNA was prepared with Trizol (Life Technology ...
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Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Library preparation using the NEB Next Ultra Library Prep Kit (New England Biolabs, Frankfurt am Main, Germany) and sequencing on Illumina Novaseq6000 platform was carried out by Novogene (Cambridge ...
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bioRxiv - Neuroscience 2020Quote: ... The RNA sequencing library was generated using NEBNext® Ultra RNA Library Prep Kit (New England Biolabs) according to manufacturer’s protocols ...
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bioRxiv - Neuroscience 2021Quote: ... and library prep performed using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB #E7760) and sequenced on an Illumina HiSeq 3000/4000 ...
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bioRxiv - Neuroscience 2019Quote: The NEBNext Ultra II Directional Library Prep Kit for Illumina (#NEBE7760L; New England Biolabs Inc., Ipswich, MA) was used on 25 ng of total RNA for the preparation of strand-specific sequencing libraries from each TRAP-isolated RNA sample (input ...
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bioRxiv - Physiology 2021Quote: ... In vitro transcription was performed using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, USA) with a customized ribonucleoside blend of GTP (1.5 mM) ...
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bioRxiv - Neuroscience 2020Quote: ... Libraries were quantified with NEBNext Library Quant Kit for Illumina (Catalog # E7630, New England BioLabs, Ipswich, MA) before sequencing them on a NextSeq500 (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... Site-directed mutagenesis was carried out using the Quick-Change Site Directed Mutagenesis Kit (New England Biolabs) as per manufactures instructions.
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Cre/LoxP-HBV plasmids generating recombinant covalently closed circular DNA genome upon transfectionbioRxiv - Microbiology 2020Quote: ... PCR was performed according to manufacturer’s protocol (Phusion High-Fidelity PCR kit, New England Biolabs, Ipswich, MA).
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bioRxiv - Synthetic Biology 2021Quote: ... Libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs, E7770), and sequenced on a NextSeq 550 single-end 75 cycles high output with v2.5 chemistry ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg of RNA isolate was subjected to rRNA removal with the NEBNext rRNA Depletion Kit (NEB) using the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2020Quote: ... K558E and K579E substitutions in FLYC1 were generated using Q5 Site-Directed Mutagenesis Kit (New England BioLabs) according to the manufacturer’s instruction and confirmed by full-length DNA Sanger sequencing.
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bioRxiv - Microbiology 2021Quote: ... cDNA was generated for all serially diluted RNA samples using LunaScript RT SuperMix Kit (New England BioLabs). Sufficient volume was prepared to perform duplicates for each method at each dilution ...