Labshake search
Citations for New England Biolabs :
4401 - 4450 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2023Quote: ... and resuspended in 20 µL H2O.Preparation of sequencing libraries was performed using NEBNext Ultra II kit (NEB) with 7 PCR cycles for library amplification ...
-
bioRxiv - Neuroscience 2023Quote: ... . Libraries for Illumina short-read sequencing were prepared using the NEBNext Ultra II RNA library kit (NEB) according to manufacturer instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... M Y229A and MT205D substitutions were generated using the Quick change directed mutagenesis kit (New England Biolabs), as recommended by the manufacturer.
-
bioRxiv - Molecular Biology 2023Quote: ... and all constructions and intermediates were generated using a NEBuilder HiFi DNA assembly cloning kit (E5520S, NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... A C-terminal HA tag was added using Q5 Site-Directed Mutagenesis Kit (New England Biolabs, #E0554S). For microinjection ...
-
bioRxiv - Genetics 2023Quote: ... The linearized Luc-shRunx2 vector and 1cisCXp promoter were further assembled together using Gibson Assembly Kit (NEB). A conceptual schematic of the gene circuits function is shown in Fig ...
-
bioRxiv - Cell Biology 2022Quote: ... Libraries were generated using the NEBNext UltraTM RNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA) for the Illumina system following the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... Amplification of the library was performed with components of the NEBNext Ultra II DNA kit (NEB E7645S) and a NEBNext Multiplex Oligos set (e.g. ...
-
bioRxiv - Biophysics 2023Quote: ... and/or insertions were achieved via site-directed mutagenesis (New England Biolabs Q5 site-directed mutagenesis kit) of the HH-pre-miR-31-HDV plasmid with primers listed in Table S10 ...
-
bioRxiv - Cell Biology 2023Quote: ... SHORT and LONG reporters were generated by assembly (NEBuilder® HiFi DNA Assembly Cloning Kit, E5520S, NEB) of AURKA 5’UTR ...
-
bioRxiv - Biophysics 2023Quote: ... Total RNA was extracted from cell cultures using the Monarch Total RNA Miniprep Kit (New England Biolabs) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... Libraries were generated for sequencing using the NEBNext Ultra II DNA Library Prep kit for Illumina (NEB) as per the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2023Quote: ... Libraries were generated for sequencing using the NEBNext Ultra II DNA Library Prep kit for Illumina (NEB) as per the manufacturer’s protocol ...
-
bioRxiv - Genomics 2023Quote: ... Kits LSK109 and LSK110 were obtained from ONT and Companion Module NebNext from New England Biolabs (NEB). As a minor modification ...
-
bioRxiv - Genomics 2022Quote: ... The TF sample was expressed by using a PURExpress In Vitro Protein Synthesis Kit (New England BioLabs) and analyzed in duplicate on two different PBM arrays (HK and ME ...
-
bioRxiv - Genetics 2022Quote: The construction of cavefish-tested pDEST_exorh:mGreenLantern and pDEST_exorh:mCherry was performed using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB E5520S). The original vector pDestTol2A2 (Tol2kit #394 ...
-
bioRxiv - Developmental Biology 2022Quote: Complementary DNA (cDNA) encoding for Human CD36 (RC221976) was cloned using Hifi DNA assembly kit (NEB E5520S) into the pJFRC-MUH vector (Addgene ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA), and the transcriptome libraries were sequenced on an Illumina NovaSeq 6000 system (Novogene Co ...
-
bioRxiv - Genomics 2023Quote: ... were added by using NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs, USA). Ligation products were purified by Ampure XP (Beckman Coulter ...
-
bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from 1 µg of total RNA using the LunaScript RT SuperMix kit (NEB, UK) in accordance with the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: ... Libraries were cloned using the NEBNext Ultra Directional II RNA Library Prep Kit for Illumina (NEB, E7760), following the manufacturer’s instruction ...
-
bioRxiv - Biochemistry 2023Quote: ... and Golden Gate cloning with the NEBridge® Golden Gate Assembly Kit (New England Biolabs, Ipswich, USA) according to the instructions provided by the manufacturer ...
-
bioRxiv - Biochemistry 2023Quote: ... The mutants were generated using the Q5 site-directed mutagenesis kit (New England Biolabs, Ipswich, MA, USA), and plasmids were confirmed by Sanger sequencing (LGC Genomics) ...
-
bioRxiv - Biochemistry 2023Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep kit for Illumina (NEB E7645S). Library size distribution was monitored by capillary electrophoresis (Agilent 2100 Bioanalyzer ...
-
bioRxiv - Biochemistry 2023Quote: ... extraction of genomic DNA with the Monarch® Genomic DNA Purification Kit (New England Biolabs, Ipswich, USA), Gibson assemblies with the NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs ...
-
bioRxiv - Biochemistry 2023Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following the manufacturer‘s recommendations ...
-
bioRxiv - Neuroscience 2023Quote: ... Sequencing libraries were made with the NEBNext Ultra II DNA Library Prep kit (New England Biolabs E7645L) and cleaned-up using SPRIselect beads (Beck-man Coulter B23317) ...
-
bioRxiv - Neuroscience 2023Quote: 150 ng of total RNA was applied to the NEBNext rRNA Depletion Kit (New England Biolabs, #E6310L) according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2023Quote: ... Single-end strand-specific libraries were prepared using NEBNext Ultra RNA library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Animal Behavior and Cognition 2023Quote: ... and converted this mRNA to 280-300 bp cDNA fragments using the Ultra II Directional Kit (NEB). Unique sequencing adapters were added to each cDNA library for multiplexing (NEB).
-
bioRxiv - Plant Biology 2023Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
-
bioRxiv - Genetics 2023Quote: ... Libraries were prepared using NEBNext Ultra II library preparation kits for Illumina (New England BioLabs, Ipswich, MA). Each pool was indexed with a unique NEBNext i7 adapter and an i5 adapter containing a degenerate barcode and PCR amplified with 12 cycles ...
-
bioRxiv - Microbiology 2023Quote: ... Eluted RNA was depleted from ribosomal RNA using NEBNext rRNA Depletion Kit (Bacteria, New England Biolabs, MA) then used as template to generate cDNA using NEBNext Ultra RNA Library Prep Kit for Illumina (England Biolabs ...
-
bioRxiv - Cell Biology 2023Quote: ... and RNA-seq libraries were generated using the Next Ultra RNA Library Prep Kit for Illumina (NEB) as described before (Berneking et al ...
-
bioRxiv - Cancer Biology 2023Quote: RNA was isolated from cell lysates using the Monarch Total RNA Miniprep Kit (T2010S, New England BioLabs) according to the manufacturer’s recommendations ...
-
bioRxiv - Microbiology 2023Quote: ... The sgRNAs were prepared using the EnGen sgRNA synthesis kit following the manufacturer’s protocol (New England BioLabs), and then associated with EnGen Spy Cas9 NLS protein (New England BioLabs ...
-
bioRxiv - Microbiology 2023Quote: ... was constructed by assembling 3 DNA fragments using the NEBuilder HiFi DNA assembly kit (New England Biolabs). All 3 fragments were amplified from pTRL2-His-GrgA 36 using Q5 DNA polymerase (New England Biolabs) ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Libraries were prepared using the NEB Next® UltraTM DNA Library Prep Kit (New England Biolabs, U.S.A) with a target insert size of 350 nt and six PCR cycles ...
-
bioRxiv - Molecular Biology 2023Quote: ... Tm or EPEC FliCD0 constructs were generated with the Q5 site-directed mutagenesis kit (New England Biolabs) following manufacturer recommendations ...
-
bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations ...
-
bioRxiv - Molecular Biology 2023Quote: In vitro translation was carried out with a custom PURExpress ΔRF-123 ΔRibosome kit (New England Biolabs), using ∼5 pmol of 5’-biotinylated and 3’-polyadenylated mRNA as a template ...
-
bioRxiv - Microbiology 2023Quote: ... The metagenomic library was constructed using NEBNext® Ultra DNA Library Prep Kit for Illumina (NEB, USA). The samples were sequenced with the PE150 strategy on Illumina Hiseq Novaseq 6000 platform (commercial service ...
-
bioRxiv - Molecular Biology 2023Quote: RT-qPCR experiments were performed with the Luna® Universal One-Step RT-qPCR Kit (NEB, #E3005E) with a final reaction volume of 10 μL per well ...
-
bioRxiv - Molecular Biology 2023Quote: ... the HiScribe® T7 High Yield RNA Synthesis Kit from New England Biolabs (NEB E2040S, Ipswich, MA) was used with the standard RNA synthesis protocol and incubated for 4 hours ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... and lysed using the Tissue Lysis buffer from the Monarch® Genomic DNA Purification Kit (NEB, #T3010S) with double the volume of lysis buffer and a 1 h incubation at 56 C ...
-
bioRxiv - Neuroscience 2023Quote: ... and Cttn-VC155 were generated by NEBuilder® HiFi DNA Assembly Cloning Kit (# E5520S, New England BioLabs), which enables the assembly of multiple inserts within a linearized vector in a one-step PCR reaction ...
-
bioRxiv - Neuroscience 2023Quote: ... After quantification of individual libraries by q-PCR using the NEBNext library quant kit (New England BioLabs), all the samples were pooled in an equimolar manner ...
-
bioRxiv - Microbiology 2023Quote: We performed RT-qPCR on select genes using the Luna Universal One-Step Kit (New England Biolabs) and QuantStudio 5 (Thermo) ...
-
bioRxiv - Genetics 2024Quote: ... Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA, Catalog #: E7530L) following the manufacturer’s recommendations ...
-
bioRxiv - Developmental Biology 2024Quote: ... The amplified pools were subjected to PCR product purification by Monarch PCR and DNA Cleanup Kit (NEB). The purified cDNAs were cloned by NEB PCR Cloning Kit and individual clones were isolated and sequenced (Azenta Life Sciences).