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Citations for New England Biolabs :
4301 - 4350 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and effectors were cloned using the Gibson Cloning Assembly Kit (New England BioLabs, catalog no. NEB-E5510S). After cloning and sequencing ...
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bioRxiv - Developmental Biology 2024Quote: ... Whole cells were processed with the “NEBNext Single Cell/Low Input RNA Library Prep” kit (NEB #E6420) by following manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: Suitable primer pairs (Table S5) were designed utilizing the Q5 site-directed mutagenesis kit (New England Biolabs) to generate mutated amidases ...
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bioRxiv - Neuroscience 2024Quote: Blood corticosterone levels were measured using the Mouse corticosterone Enzyme-linked immunosorbent assay (ELISA) kit (BIOLABS, USA) by collecting blood from wild-type mice ...
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bioRxiv - Neuroscience 2024Quote: ... and effectors were cloned using the Gibson Cloning Assembly Kit (New England BioLabs, catalog no. NEB-E5510S). After cloning and sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... and libraries prepared using the NEBNext UltraII Directional RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... The concentration of the purified amplicons was quantified by qPCR using the NEBNext Illumina Quant kit (NEB). The forward and reverse reads were demultiplexed and fused barcodes were quantified through alignment against custom barcode and primer sequences using Bowtie2 (V2.3.4.1 ...
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bioRxiv - Developmental Biology 2024Quote: ... Ribosomal RNA was depleted from the total RNA using the NEBNext rRNA Depletion kit (New England Biolabs), followed by the conversion of the remaining RNA into an Illumina sequencing library using the NEBNext Ultra Directional RNA Library Prep kit (New England Biolabs) ...
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bioRxiv - Immunology 2024Quote: ... DNA library construction was performed using the NEBNext Ultra II DNA Library Prep Kit from NEB (E7645L). The libraries were sequenced on Illumina NovaSeq 6000 system (50 bp paired-end ...
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bioRxiv - Developmental Biology 2024Quote: ... After PCR amplification or plasmid digestion product was purified with Monarch PCR clean-up kit (NEB, #T1030s) and used to produce the DIG-labeled single-stranded RNA probes using the DIG RNA labeling kit (Roche ...
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bioRxiv - Cell Biology 2024Quote: ... Reverse transcription reactions were performed using a LunaScript RT SuperMix Kit (#E3010L, New England Biolabs, Ipswich, MA) with 500 ng of total RNA ...
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bioRxiv - Cell Biology 2024Quote: ... The synthesized probes were then purified using the Monarch® PCR & DNA Cleanup Kit (New England BioLabs) according to the manufacturer’s instructions and the quality was verified on denaturing 12-16% polyacrylamide gels.
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bioRxiv - Immunology 2024Quote: ... Libraries were prepared using the NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs) according to manufacturer’s instructions with the following specifications and modifications ...
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bioRxiv - Cell Biology 2024Quote: The tricistronic Gs and Gs-CFP plasmids were created using NEBuilder HiFi DNA assembly cloning kit (NEB). Gαs (or Gαs-CFP54) ...
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bioRxiv - Biochemistry 2023Quote: ... Indexed DNA libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB, Cat # E7645S) with the following specifications ...
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bioRxiv - Immunology 2023Quote: ... in vitro transcription was performed over-night with the T7 High Yield RNA polymerase IVT kit (NEB) for 37°C overnight ...
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bioRxiv - Synthetic Biology 2023Quote: ... Quick Blunting Kit and the T4 DNA Ligase enzyme were purchased from New England BioLabs (NEB, Germany). The plasmid extraction kit was purchased from Qiagen GmbH (Hilden ...
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bioRxiv - Genetics 2023Quote: ... RNA library preparation was done using a NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) on a Bravo WS automation system (Agilent) ...
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bioRxiv - Genomics 2023Quote: Libraries were built on beads using the NEBNext Ultra II DNA Library Prep kit (NEB, Cat # E7645L). Biotin pull down was initiated py pipetting 20 μL Dynabeads My-One Streptavidin T1 beads (ThermoFisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... 2 μg of total RNA was converted into cDNA with LunaScript RT SuperMix kit (New England Biolabs). The cDNAs were used as templates for real-time PCR and ran on StepOnePlus real-time PCR system (Applied Biosystems ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Sequencing libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Inc.) at Novogene and sequenced on an Illumina NovaSeq 6000 (2 × 150 bp ...
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bioRxiv - Genomics 2023Quote: ... An NGS library was generated using an NEB Ultra II DNA Library Prep kit (NEB, Ipswich, MA) with an Illumina compatible y-adaptor ...
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bioRxiv - Genetics 2023Quote: ... 1x106 HEK293T cells were collected and their gDNA was extracted using the NEB Monarch kit (T3010S, NEB) according to the manufacturer’s protocol ...
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HOIL-1L deficiency induces cell cycle alteration which causes immaturity of myocyte and fibrogenesisbioRxiv - Cell Biology 2023Quote: ... Sequencing libraries were prepared using a NEBNext Ultra II RNA Library Kit for Illumina (New England Biolabs) with the NEBNext Poly (A ...
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bioRxiv - Cell Biology 2023Quote: ... We created this deletion using a Q5 Site-Directed Mutagenesis kit (New England Biolabs, Cat. No: E0554S) with subsequent sequence verification ...
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bioRxiv - Genomics 2023Quote: ... using poly(A) selection and the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs). RNA-seq (2 × 100 nt paired-end reads ...
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bioRxiv - Genomics 2023Quote: ... Short-read libraries were prepared with the NEBNext Ultra II FS DNA Library Prep kit (NEB E7805), and sequenced 150 bp paired-end on an Illumina HiSeq3000 ...
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bioRxiv - Microbiology 2024Quote: ... SACOL2602 and SACOL2601 were individually deleted from pRab11-tspA1-328-pep86_2603_2602_2601 using the Q5 site-directed mutagenesis kit (NEB). Oligonucleotides are listed in Table S1.
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bioRxiv - Genomics 2024Quote: ... and used for the cloning of PCR fragments with a Gibson Assembly cloning kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: MeRIP sequencing (MeRIP-seq) libraries were prepared using the Ultra II directional RNA library preparation kit (NEB) as per manufacturer’s instructions for already fragmented samples ...
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bioRxiv - Immunology 2022Quote: ... and subjected to in vitro transcription (IVT) using HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S), then treated with ExoSAP-IT (Affymetrix ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA-seq libraries were prepared using NEBNext Ultra Directional RNA library preparation kit for Illumina (NEB #E7760). Library quality was checked by Agilent BioAnalyzer 2100 using the DNA 1000 kit (Part number 5067-1504 ...
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bioRxiv - Neuroscience 2023Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... Sequencing libraries were generated using NEBNext Ultra TM RNA Library Prep Kit for Illumina (NEB, Ipswich, MA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were depleted three times of polyA(+) RNA using the Magnetic mRNA isolation kit (New England Biolabs), each time keeping the supernatant ...
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bioRxiv - Microbiology 2022Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... purified total RNA was reverse transcribed using the ProtoScript II First Strand cDNA Synthesis Kit (#E6560, NEB) and a 1:1 mix of oligodT and random hexamer primers to obtain cDNA ...
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bioRxiv - Microbiology 2023Quote: Total RNA from cell pellets was extracted using a Monarch Total RNA Miniprep kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 5’-phosphorylated and dA-tailed with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645). Samples were ligated to adaptor oligos for multiplex sequencing (NEB ...
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bioRxiv - Microbiology 2023Quote: ... Transcript levels of individual genes were determined by the Luna Universal One-Step RT-PCR kit (NEB) using approximately 100-200 ng of total RNA per sample as input ...
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bioRxiv - Molecular Biology 2023Quote: ... Library preparation was done using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEW ENGLAND BioLabs) as described by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... The transcription of guide RNAs (gRNAs) was performed with HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... 400ng of total RNA was used for reverse transcription using LunaScript™ RT SuperMix Kit (NEB, USA). qPCR was performed using Forget-Me-Not™ EvaGreen® qPCR Master Mix (Biotium ...
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bioRxiv - Neuroscience 2023Quote: ... Generation of Adgrd1 N-terminal mutations was carried out using Q5 site directed mutagenesis kit (NE Biolabs) using manufacturers protocol ...
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bioRxiv - Genetics 2023Quote: ... Whole genome sequencing libraries were prepared using the NEBNext Ultra II Library kit (New England Biolabs, E7645L) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: Library construction was carried out using NEBNext Ultra II DNA Library Prep Kit for Illumina (E7645S, NEB). 4ng of fragmented DNA was used for end-repair/A-tailing ...
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bioRxiv - Microbiology 2023Quote: ... and cDNA was generated using an AMV first strand cDNA synthesis kit (New England Biolabs, Catalog # E6550). The segment was amplified through PCR with PstI and BamHI restriction sites incorporated onto the double-stranded cDNA using specific primers (PstI-VIGS-F and BamHI-VIGS-R ...
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bioRxiv - Molecular Biology 2022Quote: ... Ligation of the insert to the vector was performed using the Quick Ligation kit (New England Biolabs). The final vector was transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... The amplicon was inserted into miniTn7/pMQ56 using the NEBuilder HiFi DNA Assembly kit (New England BioLabs) according to the manufacturer specifications ...
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bioRxiv - Microbiology 2023Quote: ... NCPF 8977 (aggregating) and NCPF 13029 (weakly-aggregating) using Monarch RNA extraction kit (NEB cat no. T2010) as per manufacturer’s specification ...