Labshake search
Citations for New England Biolabs :
3851 - 3900 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... UDI-barcoded RNAseq libraries were generated with the NEBNext Ultra II RNA Library Prep Kit (NEB). Each library was quantified with a Qubit (dsDNA HS kit ...
-
bioRxiv - Immunology 2024Quote: ... Further modifications were performed by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (NEB). All modifications to the 2X_pX458_pSpCas9(BB)-2A-GFP_SOX17_bKO plasmid (Addgene ...
-
High-resolution chromosome-level genome provides molecular insights into adaptive evolution in crabsbioRxiv - Genomics 2024Quote: ... Sequencing libraries were constructed using the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA), following the manufacturer’s guidelines ...
-
bioRxiv - Molecular Biology 2024Quote: ... 1 µg of total RNA was reverse transcribed using LunaScript RT SuperMix Kit (New England Biolabs) according to the manufacturer’s instructions and 1 µl cDNA was used as a template for a 10 µl qPCR reaction ...
-
bioRxiv - Molecular Biology 2024Quote: ... RNA was then purified from the quenched reaction mixtures using Monarch® RNA Cleanup Kit (NEB) according to manufacturer protocols and subsequently quantified by NanoDrop.
-
bioRxiv - Bioengineering 2024Quote: ... and cDNA libraries were generated by RT-PCR using the ProtoScript II cDNA Synthesis Kit (BioLabs) according to the manufacturers’ protocols ...
-
bioRxiv - Zoology 2024Quote: ... RT-qPCR was performed using the Luna One-step RT-qPCR kit (New England Biolabs, Germany) on a 384 well-plate with 3 technical replicates per biological replicate in CFX384 Touch Real-Time PCR (Bio-Rad ...
-
bioRxiv - Genomics 2024Quote: ... Sequencing adapters were ligated using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) while DNA was bound to the beads ...
-
bioRxiv - Genomics 2024Quote: ... The recovered DNA was subjected to library construction using NEBNext Ultra DNA library prep kit (NEB). The sequencing libraries were amplified by PCR using the NEBNext Ultra II Q5 Master Mix (NEB ...
-
bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...
-
bioRxiv - Physiology 2024Quote: ... Plasmid and genomic DNA (gDNA miniprep kits were purchased from Promega. E. coli NEB 5α (NEB) was used as a host for plasmids ...
-
bioRxiv - Cell Biology 2024Quote: ... and reversely transcribed into cDNA using the ProtoScript II NEB cDNA synthesis kit (New England Biolabs).
-
bioRxiv - Developmental Biology 2024Quote: ... plasmid templates were linearized with NotI and purified using Monarch PCR purification kits (New England Biolabs). The purified templates were transcribed using mMESSAGE mMACHINE Sp6 (Thermo-Fisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... DNA was subsequently purified with a Monarch PCR & DNA Cleanup Kit (New England Biolabs, Ipswich, MA) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Val206 of Envy was mutagenized to Lys with the Q5 Site-Directed Mutagenesis Kit (NEB #E0554S) using primers listed in Table S2 ...
-
bioRxiv - Synthetic Biology 2024Quote: ... A NEB Monarch® Plasmid Miniprep Kit was used for RT-DNA extraction (New England Biolabs). Following miniprep ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... After the reaction we purified the PCR product with Monarch PCR and DNA Cleanup Kit (NEB).
-
bioRxiv - Molecular Biology 2024Quote: ... The resulting PCR product was cloned into the pCDF4 vector using the Gibson Assembly kit (NEB) and the resulting plasmid was then used to generate ATG4-gRNA transgenic flies via injection into the y1 ...
-
bioRxiv - Immunology 2024Quote: ... and subsequently replaced with the tailed rpoS DNA fragment using the HiFi DNA assembly kit (NEB). Following the assembly reaction ...
-
bioRxiv - Microbiology 2024Quote: ... cDNA was synthesised according to the protocols of the LunaScript RT Supermix Kit (New England Biolabs) and for qPCR amplification ...
-
bioRxiv - Plant Biology 2020Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (NEB), following the manufacturer’s recommendations and sequenced on Illumina Hiseq platform and 150 bp paired-end reads were generated.
-
bioRxiv - Microbiology 2020Quote: ... and ftsH target sequences was carried out in vitro using a Q5 mutagenesis kit (New England BioLabs) with specified primers (S1 Table) ...
-
bioRxiv - Microbiology 2020Quote: ... Total RNA was collected at 9 h post-IFN treatment using Monarch Total RNA Miniprep Kits (NEB). RNA was prepped for RNA sequencing submission using the NEBNext Poly(A ...
-
bioRxiv - Synthetic Biology 2021Quote: ... PCR reactions were purified with Monarch DNA Cleanup Kit columns (T1030; New England Biolabs; Ipswich, MA, USA) per the manufacturer’s protocol and eluted in 20 µL nuclease-free water.
-
bioRxiv - Genomics 2021Quote: ... 1μl RT Adapter (110nM; ONT Kit) and 1.5μl T4 DNA Ligase (2M U/ml New England BioLabs). The ligation reaction was carried out for 10min at RT ...
-
bioRxiv - Cancer Biology 2021Quote: ... ΔN1/2/3 and ΔDAD mutants were generated by Q5® Site-Directed Mutagenesis Kit (NEB, E0554S) with HOXB13 WT or NCOR1 WT as a template ...
-
bioRxiv - Biophysics 2020Quote: ... FR-Q and FR-MQ were made using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) with pBad-FR and pBad-FR-M ...
-
bioRxiv - Cancer Biology 2021Quote: ... RNA was transcribed to cDNA using NEBNext Ultra™ RNA Library Prep Kit for Illumina (NEB E7530). RNA sequencing was performed using single-end 75 bp reads on an Illumina NextSeq500 ...
-
bioRxiv - Molecular Biology 2019Quote: ... RNA-seq libraries were constructed using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770) following manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... Sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) with size selection for ~200 bp insert DNA ...
-
bioRxiv - Cell Biology 2020Quote: ... Site directed mutagenesis to generate GATA4 V266M was performed with the Q5 Site-Directed Mutagenesis Kit (NEB) as per the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... Sequencing library preparation was performed using the NEBNext Ultra DNA library prep kit for Illumina (NEB, E7370S), following manufacturer instructions ...
-
bioRxiv - Cell Biology 2020Quote: Sequencing library was prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB E7770S) according to manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... and libraries were prepared using NEBNext UltraII Directional RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer instructions ...
-
bioRxiv - Immunology 2021Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
-
bioRxiv - Genetics 2021Quote: ... Libraries were then cloned using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB), following the recommended kit protocol and sequenced on a NovaSeq 6000 – SR100 (Illumina).
-
bioRxiv - Genetics 2020Quote: ... We performed RNA purification and genomic DNA removal using the Monarch Total RNA Miniprep Kit (T2010S, NEB) following manufacturer’s instructions for purifying TRIzol-extracted samples ...
-
bioRxiv - Microbiology 2021Quote: ... RT was performed using 1 μg of RNA using LunaScript™ RT SuperMix Kit (New England BioLabs) per manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: ... Total blood RNA from DBS was extracted using Monarch Total RNA Miniprep Kit (New England Biolabs, USA) with a modified protocol ...
-
bioRxiv - Molecular Biology 2020Quote: ... pUHES:UmPit2+SP:mCherry with its natural signal peptide was generated via NEBuilder HiFi DNA Assembly Cloning Kit (NEB) by combining PCR-amplified UmPit2+SP:mCherry using primers 2127 + 2128 and PCR-amplified pUHESdest using primers 2125 + 2126 without UhAvr1 signal peptide ...
-
bioRxiv - Microbiology 2019Quote: ... The sequencing library was prepared using a NEBNext Ultra II RNA library prep kit (New England BioLabs) and quantified using a NEBNext Library Quant kit for Illumina (New England BioLabs) ...
-
bioRxiv - Microbiology 2019Quote: ... Library preparation was carried out using NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs) following manufacturers guidelines ...
-
bioRxiv - Molecular Biology 2021Quote: ... This is then followed by second- strand synthesis and NEB ultra-directional RNA sequencing kit (NEB #E7760).
-
bioRxiv - Molecular Biology 2021Quote: ... Derivatives of pEAI423 containing mutations in EXO1 were constructed with the Q5 mutagenesis kit (New England Biolabs) using pEAI423 as template and the oligonucleotides shown in Table S7 ...
-
bioRxiv - Developmental Biology 2021Quote: ... ChIP-seq libraries were prepared using NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs) and sequenced using the HiSeq 50 cycle single-end reads protocol on the HiSeq 2500 system.
-
bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were generated using NEBNext® Ultra™ DNA Library Prep Kit for Illumina (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample [49] ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... RNA sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #E7530). NEBNext Poly(A ...
-
CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... were introduced in full-length ezh2 cDNA by the Q5® Site-Directed Mutagenesis Kit (NEB; E0544S). Resulting plasmids were validated by Sanger sequencing ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... followed by library preparation using the NEBNext Ultra II library kit (New England BioLabs Ltd, Hitchin, UK). The final library pools were generated and sequenced on the Illumina X ten platform (Illumina ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Libraries were prepared using the NEBNext® Ultra™ II DNA Kit (New England Biolabs, Massachusetts, USA). Samples with high molecular weight DNA (>1000 bp ...