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Citations for New England Biolabs :
351 - 400 of 4152 citations for Pops Hrms Hch Clean Up Spike 13C6 99% 50X Stock 1 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... 2.5% (v/v) 1 mg/mL bovine serum albumin (NEB #B9200S), 5% (v/v ...
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bioRxiv - Systems Biology 2023Quote: ... and 1 μL T4 DNA Ligase (400,000 U/mL, NEB, #M0202S). Ligation occurred at 25°C for 2 hours ...
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bioRxiv - Cell Biology 2023Quote: ... and 200 µg/ml RNAse A (New England Biolabs #T3018-1)) for four hours on ice ...
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bioRxiv - Cell Biology 2023Quote: ... 1mM DTT) before adding 15 µl 1 mg/ml Streptavidin (NEB) before immediate wash with DLB-C-T buffer (DLB supplemented with 1 mg/ml α-casein ...
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bioRxiv - Immunology 2024Quote: ... 1 µL of 2000 U/mL DNase I (New England Biolabs) was added to bring the final reaction volume to 10 µL ...
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bioRxiv - Bioengineering 2024Quote: ... pH 8.0) containing 8 units mL−1 proteinase K (NEB, P8107S) at 37 °C for 4 h ...
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bioRxiv - Microbiology 2021Quote: ... Firefly luciferase (FLuc) RNA served as the spike in control and was generated using HiScribe T7 Quick RNA Synthesis kit (NEB, Ipswich, MA, USA) using linearized plasmid DNA encoding FLuc gene as template ...
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bioRxiv - Immunology 2021Quote: ... except after the Ni-NTA step it was concentrated (1 mg/ml in 0.2 ml) and shaved with endoglycosidase D (10 μl, 100 unit) (New England BioLabs) for 16 hrs at 4°C prior to gel filtration ...
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bioRxiv - Genetics 2021Quote: ... PCR products were cleaned up using Exo-AP treatment (7.5 µl PCR product mixed with 0.5 µl Exonuclease-I (NEB), 0.5 µl Antarctic Phosphatase (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... 6×50 µl PCR reactions were set up using NEBNext Ultra II Q5 master mix (New England Biolabs) with 5 nM oligos as template ...
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bioRxiv - Immunology 2022Quote: ... PCR reaction was set up according to NGS protocol of NEBNext UltraTM II Q5 Master Mix (NEB, Cat.M0544S). PCR products were purified by AMPure XP beads (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR reactions were set up using primers specific to either CRISPRi or CRISPRa sequences using Phusion polymerase (NEB) according to the manufacturer’s instructions and using 3 different conditions to minimize amplification bias (HF buffer ...
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bioRxiv - Molecular Biology 2023Quote: The amplicons from the Q5 PCR step were cleaned up using Monarch® PCR & DNA Cleanup Kit (NEB). The purity and size of the DNA bands were visualized by running a 1% agarose gel ...
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bioRxiv - Bioengineering 2023Quote: All qPCR reactions were set up using 7.5 µl of 2 x Luna Universal qPCR master mix (NEB), 0.375 pmol forward primer ...
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bioRxiv - Biochemistry 2023Quote: ... and UP* in the list below) were generated at a concentration of 40 μM in NEBuffer#2.1 (NEB) buffer ...
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bioRxiv - Cell Biology 2019Quote: ... 0.25μl of each primer (from 25μM stock) and 4.5μl of Luna Universal Master Mix (New England Biolabs). The q-RT-PCR reactions were run on the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2019Quote: ... A 10-fold dilution of Nt.BstNBI was prepared by dissolving the stock enzyme in Diluent A (NEB) supplemented with 0.1 % Triton X-100 ...
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bioRxiv - Microbiology 2020Quote: ... Stock solutions of SNAP-Cell® TMR-Star or SNAP-Cell® 647-SiR (New England Biolabs) in DMSO were diluted to 4 μM in complete medium ...
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bioRxiv - Neuroscience 2023Quote: ... 1 mM EDTA pH 8.0 (Roth) and 1:100 Proteinase K (New England Biolabs 20 mg/ml)) in a 2 ml Eppendorf tube for at least 24 hours at RT in the dark ...
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bioRxiv - Microbiology 2023Quote: ... A 1 ml aliquot of each phage was treated with 1 µl DNase (New England Biolabs, USA) at 37 ⁰C for 40 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Purified HTO sequencing libraries were then amplified by PCR (1μl clean HTO cDNA, 25μl 2X NEBNext Master Mix (NEB #M0541), 10 µM SI-PCR and D701or D704 primers performed dial out PCR (98°C (10 sec) ...
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bioRxiv - Developmental Biology 2020Quote: ... The supernatant was removed using a magnetic separator and washed once with 1 mL NEBuffer 2 and resuspended in 115 mL of Ligation reaction with Quick Ligase buffer (NEB), 6,000 U of Quick Ligase (NEB ...
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bioRxiv - Developmental Biology 2020Quote: ... the concentration of eluted MBP-mintbody was adjusted to 1 mg/ml and digested with 30 μg/ml Factor Xa (New England Biolabs) for 24 h ...
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bioRxiv - Microbiology 2022Quote: ... Institut Jacques Boy), recombinant human IL-2 (rhIL2, 10 U/ml, Miltenyi) and DNase (1 U/ml, New England Biolabs), washed and enumerated ...
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bioRxiv - Genomics 2023Quote: ... and washed twice with 2 mL of CLB containing Superase-In and 1% v/v of 20 mg/mL molecular grade BSA (NEB). After the final wash ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by adding 1 μL of proteinase K (800 units/ml) (NEB) and incubating at room temperature for 10 minutes before being run on a 1.7% agarose gel with a 100 bp ladder for 35 minutes.
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bioRxiv - Molecular Biology 2021Quote: ... and together with the addition of apyrase (25 mU mL−1, NEB). No CID2886111 was supplied in the purification ...
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bioRxiv - Microbiology 2020Quote: ... 0.8 mg ml-1 bovine serum albumin (BSA, New England Biolabs GmbH), 0.2 mM of each dNTP (Bioline ...
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bioRxiv - Genomics 2020Quote: ... The supernatant was loaded on four 1-ml chitin columns (NEB S6651S). Columns were washed with 10 ml HEGX ...
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bioRxiv - Microbiology 2021Quote: ... Immediately followed by enzymatic treatment of 1 mg/mL proteinase K (NEB) at 60°C for 60 min ...
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bioRxiv - Microbiology 2022Quote: ... 150 µl Proteinase K (800 units ml-1; New England Biolabs #P8107S), 138 µl 20% SDS ...
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bioRxiv - Synthetic Biology 2023Quote: ... Following one-hour outgrowth in 1 mL of SOC medium (NEB #B9020S) at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.3% Tween 20) with 1 μL proteinase K (800 U/ml, NEB) in 96 well plates ...
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bioRxiv - Biochemistry 2023Quote: ... 1 M NaCl and 8 units/ml Proteinase K (New England Biolabs) in 1x PBS] ...
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bioRxiv - Biophysics 2023Quote: ... The lysates were clarified and loaded on 1 mL chitin resin (NEB) and washed with 1 M KCl ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were grown in 1 mL SOC Outgrowth Medium (B9020, NEB) for 2 h in a standard glass tube at 30 °C with 160 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... and reaction samples were loaded onto 1 ml of amylose resin (NEB). Proteins in the flow-through fractions were further purified by gel filtration chromatography (Superdex 200pg ...
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bioRxiv - Cancer Biology 2024Quote: ... prior to incubation with 1% SDS and proteinase K (20mg/mL, NEB) to inactivate and degrade pA-Tn5 ...
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bioRxiv - Molecular Biology 2022Quote: ... Up to 5 μg of gDNA was digested with 15 U of SalI-HF® enzyme (NEB, Cat# R3138L) for 3 hr at 37°C and separated on 0.9% agarose gel for 16 hr at 50 V ...
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bioRxiv - Developmental Biology 2019Quote: ... library amplification was performed by setting up a PCR reaction (50 μl) with 1X Phusion HF master mix (NEB), 0.2 uM universal primer ...
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bioRxiv - Microbiology 2022Quote: ... A volume of ultrapure water needed to make the reaction up to 37.5μL after the addition of rCutsmart (NEB B6004S) and PspGI (NEB R0611 ...
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bioRxiv - Genomics 2022Quote: ... and water to make up 49 µl) for three hours at 37°C after which 3 µl NlaII (NEB) was added and the reaction incubated at 37°C for a further three hours ...
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bioRxiv - Microbiology 2023Quote: ... The thermal cycle of the RT-qPCR machine was set up based on NEB #M3003 instruction manual (NEB, UK). Transcription values (Ct ...
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bioRxiv - Microbiology 2023Quote: ... The thermal cycle of the RT-qPCR machine was set up based on NEB #M3003 instruction manual (NEB, UK). Transcription values (Ct ...
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bioRxiv - Genetics 2023Quote: ... 6.5 μL of cleaned-up DNA was used in each PCR1 amplification with Q5 High-Fidelity DNA Polymerase (NEB) for 15 cycles ...
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bioRxiv - Microbiology 2023Quote: ... Reactions were set up as per manufacturer’s instructions with the addition of 4 units of Murine RNase Inhibitor (NEB) and 5% DMSO ...
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bioRxiv - Molecular Biology 2023Quote: ... in 1.5 Kbp windows and then searched 20 bp sequences up- and downstream of each SNP for RE recognition sites using NEBcutter V2.0 (New England BioLabs; http://nc2.neb.com/NEBcutter2/index.php) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the resulting cell pellet was resuspended in 1 mL of RNA Protection Buffer (1× NEB DNA/RNA Protection Reagent, 1% (w/v) polyvinylpyrrolidone-40 ...
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bioRxiv - Synthetic Biology 2019Quote: ... 0.5 µl of 1 mg mL−1 purified bovine serum albumin (1:20 dilution in dH2O of BSA, Molecular Biology Grade 20 mg ml−1, NEB cat. B9000), 0.25 µl of T4 DNA ligase at 400 U µL−1 (NEB cat ...
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bioRxiv - Bioengineering 2021Quote: ... 1:15 and 1:30 (PEI-AuNCs:siRNA)) were incubated with RNase H (10μg/ml, New England BioLabs, M0297L) at 37°C for 30 minutes ...