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Citations for New England Biolabs :
151 - 200 of 4152 citations for Pops Hrms Hch Clean Up Spike 13C6 99% 50X Stock 1 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... We purified and enriched mRNA from 1 ug of total RNA using the NEBNext Poly(A) Magnetic Isolation Module (New England Biolabs, catalog no. E7490L). RNA fragmentation ...
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bioRxiv - Synthetic Biology 2021Quote: Glycerol stocks of NiCo21(DE3) cells (New England Biolabs) transformed with each GFP variant were streaked onto LB agar plates with added ampicillin (100 μg/ml ...
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bioRxiv - Biochemistry 2023Quote: ... 2 uL dNTP Solution Mix (10 mM stock, NEB), 2 μl Taq DNA ligase (80 U ...
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bioRxiv - Molecular Biology 2021Quote: ... Cleaned up products were digested using XbaI restriction enzyme (NEB) in 1x CutSmart buffer and run on a 1.5% agarose gel with 1x SYBR Safe (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... mRNAs pull-up was performed using Magnetic Isolation Module (NEB). All libraries were mixed into a single tube with equal molarity ...
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bioRxiv - Genetics 2023Quote: ... seven independent reactions were set up where the EcoRI (NEB), PsiI (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... Amplified libraries were cleaned up using Sample Purification Beads (NEB) included in the kit ...
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bioRxiv - Microbiology 2021Quote: ... acnes lysates were incubated with 0.5 ug plasmid DNA together with different buffers (NEB) supplemented with or without ATP or SAM ...
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bioRxiv - Genomics 2023Quote: ... The resulting DNA fragments were then either methylated using non-specific adenine EcoGII methyltransferase (New England Biolabs, high concentration stock 2.5e4 U/mL) or left unmethylated ...
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bioRxiv - Microbiology 2023Quote: ... A 1 ml aliquot of cells was incubated with 1 μl M13KO7 (1011 plaque-forming units per ml, pfu/ml) (NEB) for 10 min on ice to allow attachment ...
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bioRxiv - Bioengineering 2020Quote: ... we use Western Blot to detect spike protein with/without treatment of PNGase F (NEB). 100 ng particles were incubated with Glycoprotein Denaturing Buffer at 98°C for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-5 μM SNAPf fused activator was incubated in a 1× PBS solution containing up to threefold excess of SNAP-surface 649 (New England BioLabs, #S9159S), 1 mM DTT ...
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bioRxiv - Cell Biology 2020Quote: ... Endogenous mRNA was removed by treating the pooled fractions (200 μL) with 2.4 μL CaCl2 (40 mM stock) and 1 μL micrococcal nuclease (New England BioLabs M0247S) at room temperature for 10 min ...
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bioRxiv - Microbiology 2020Quote: ... were reconstituted by mixing 1 µl of 100 µM stocks of the forward and reverse strands for each guide with 1 µl of 10x ligation buffer (NEB), 0.5 µl T4 polynucleotide kinase (NEB ...
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bioRxiv - Neuroscience 2023Quote: ... 3 µL of RNA from 4 x 96 well plates was transferred to a 384 well plate with 3 µL of master mix containing 1 µL of a 10 mM stock of dNTPs (NEB Catalog# N0447L ...
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bioRxiv - Synthetic Biology 2021Quote: ... The reaction was set up using a commercial kit (PURExpress NEB) according to user guidelines ...
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bioRxiv - Microbiology 2021Quote: ... qPCR reactions were set-up using Luna qPCR Master Mix (NEB) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were washed and taken up in 1.2x CutSmart buffer (NEB). SDS was added to a concentration of 0.1 % and the cells were incubated (65 °C ...
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bioRxiv - Microbiology 2021Quote: ... cleaned up using the Monarch PCR Purification kit (New England Biolabs), and used in a Gibson assembly reaction (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... The DNA was cleaned up with the NEB Monarch kit (NEB), eluted in 12 μl elution buffer ...
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bioRxiv - Neuroscience 2023Quote: ... cleaned up by using Monarch RNA Cleanup Kit (New England Biolabs), and finally eluted in 30 µl nuclease-free water ...
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bioRxiv - Microbiology 2020Quote: ... RecA was diluted from concentrated stock (New England Biolabs M0249L) into Storage Buffer (50 mM phosphate/citrate buffer at pH 7 ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 ug from each cell line was digested with 25 units EcoRI (New England Biolabs) in 100 ul total volume ...
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bioRxiv - Biochemistry 2022Quote: ... 2 ug of DNA was treated with uracil DNA glycosylase (UDG, New England Biolabs, Inc.) for 60 minutes at 37°C with gentle shaking ...
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bioRxiv - Molecular Biology 2023Quote: ... Four ug of fragmented gDNA was digested with 20 U of RNase H (NEB #M0297S) at 37°C for 6 hours to serve as a negative control ...
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bioRxiv - Cell Biology 2021Quote: ... 1% NP-40, 1 mM DTT, 0.1 mg/ml cycloheximide, 1.25 mg/ml heparin, 400 U/ml NEB murine RNase inhibitor) and dounced ...
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bioRxiv - Synthetic Biology 2021Quote: ... Reagents added were 1 μl of 1 mg/ml BSA (diluted from 20 mg/ml - NEB), 1 μl T4 DNA ligase buffer (NEB or Thermo Fisher Scientific) ...
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bioRxiv - Genomics 2022Quote: ... 1 mg/ml Proteinase K (NEB) and 1 mM Biotin
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bioRxiv - Genomics 2022Quote: ... 1% BSA (20 mg/ml, NEB)) + hash oligos (1 uM ...
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bioRxiv - Biophysics 2023Quote: ... Streptavidin (1 mg/mL, NEB N7021S) was diluted 1:10 in DPBS and applied to passivated flow chambers for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: Linker ligation at 3’ ends was set up per sample after washes following alkaline phosphatase treatment by preparing a T4 RNA ligase 1 (NEB, cat. #M0204S) reaction in 40μL following the manufacturers’ instructions with 100pmol of radiolabeled L32 RNA linker ...
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bioRxiv - Genomics 2020Quote: ... 1.5 ug of assembled array was utilized as input for methylation reactions with the non-specific adenine EcoGII methyltransferase (New England Biolabs, high concentration stock; 2.5E4U / mL). For the naked DNA controls ...
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bioRxiv - Synthetic Biology 2022Quote: ... Eight separate 50 μL PCR reactions were set up with Q5 (NEB) using 2.5 μL of the diluted midiprep as the template and with the primers Orange_NGS_F and R ...
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bioRxiv - Biophysics 2019Quote: ... PCR reactions were set up using 2x Q5 mastermix (NEB, cat# M0492), 10 ng of plasmid template ...
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bioRxiv - Developmental Biology 2022Quote: ... and then cleaned up using the Monarch PCR Cleanup kit (T1030L, NEB). Digested PCR products were then ligated to the digested pcs2+MCS-P2A-sfGFP plasmid using T4 DNA ligase (M0202S ...
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bioRxiv - Biophysics 2021Quote: PCR reactions were set up using 2x Q5 mastermix (NEB, cat#M0492), 10 ng of plasmid template ...
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bioRxiv - Microbiology 2020Quote: ... qPCR reactions were set up using Luna Universal qPCR Master Mix (NEB) following the manufacturer's protocol ...
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bioRxiv - Microbiology 2022Quote: ... Products were PCR cleaned up with Monarch PCR & DNA cleanup kit (NEB), ligated into pCR2.1-TOPO vector (Thermo fisher ...
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bioRxiv - Microbiology 2022Quote: ... and cleaned up through the Monarch PCR & DNA Cleanup spin columns (NEB). CPER assembly was performed as previously described by combining 0.05 pmol of each fragment in a 50 μL reaction containing 2.5 U PrimeSTAR GXL DNA polymerase (Takara Bio ...
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bioRxiv - Synthetic Biology 2023Quote: ... and nuclease-free H2O up to 20 μL (New England Biolabs, USA). The reaction was performed in 30 cycles of 37 °C for 5 min followed by 16 °C for 5 min and finalized by incubation at 60 °C for 5 min ...
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bioRxiv - Genomics 2022Quote: ... 3 ug of input DNA was dephosphorylated with Quick CIP (New England Biolabs, cat no M0508). Following enzyme inactivation with alkaline phosphatase ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were further quenched by the addition of 0.2 ug of RNAse A (New England Biolabs) and incubation at 37 °C for 20 min ...
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bioRxiv - Genomics 2019Quote: ... we used the miRNA quantifications from all brain libraries with all starting amounts using both batches (total of 99 libraries, 19 for the Clontech, NEXTflex, Deduped and Fivepercent methods and 10 for Illumina and 13 for NEB). We normalized the data using the DESeq2(40 ...
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bioRxiv - Molecular Biology 2023Quote: ... fixed nuclei resuspended in 171 uL SPBSTM were mixed with 99 uL NTP buffer and 30 uL T7 polymerase mix (New England Biolabs) and incubated in a 1.5 mL LoBind tube (Eppendorf ...
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bioRxiv - Biochemistry 2022Quote: ... 10 u/μL T4 DNA ligase (stock 400 u/μL, NEB). No difference in yield was found between the ligation splints ...
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bioRxiv - Cancer Biology 2019Quote: ... CviQI restriction enzyme (RE; 2.5U; 10U/μl stock, New England BioLabs), the TERT assay ...
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bioRxiv - Genomics 2021Quote: SAMOSA was performed on remodeled arrays as well as unremodeled arrays and unassembled DNA controls using the non-specific adenine EcoGII methyltransferase (New England Biolabs, high concentration stock 2.5e4 U/mL) as previously described (Abdulhay et al. ...
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bioRxiv - Genomics 2023Quote: ... and single colonies were picked 1 day later to grow up and extract plasmid DNA using a Monarch Plasmid Miniprep Kit (New England Biolabs, Cat. No. T1010L). Extracted plasmid DNA was sequence confirmed via long-read Nanopore sequencing (Primordium Labs ...
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bioRxiv - Immunology 2021Quote: ... The kRSV-DB1-QUAD plasmid and spike insert were digested with the enzymes AatII and SalI (NEB) and ligated with T4 DNA ligase (NEB ...
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bioRxiv - Biophysics 2021Quote: ... Complexes were prepared by incubating spike ectodomain preparations with either ACE2 (residues 18–615, New England Biolabs), VH ab8 ...