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Citations for New England Biolabs :
251 - 300 of 4152 citations for Pops Hrms Hch Clean Up Spike 13C6 99% 50X Stock 1 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Assays were set-up using PURExpress in vitro protein synthesis kit (New England Biolabs) with a cloned full-length DHFR template ...
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bioRxiv - Bioengineering 2024Quote: ... qPCR reactions were set up using the Luna Universal qPCR Master Mix (NEB M3003S). Reactions included 6 µL of Luna Mastermix ...
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bioRxiv - Genomics 2020Quote: ... 2 μL 10 mM dGTP and 10 mM dTTP (stock solutions: NEB, N0446), 10 μL 10x T4 DNA Ligase Reaction Buffer (NEB B0202S) ...
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bioRxiv - Microbiology 2020Quote: ... A final concentration of 2.5x SYBR Green I (10000x stock, New England Biolabs) was applied to visualize DNA ...
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bioRxiv - Genomics 2020Quote: ... 2 μl 10 mM dGTP and 10 mM dTTP (stock solutions: NEB, #N0446), 10 μl 10x T4 DNA Ligase Reaction Buffer (NEB #B0202S) ...
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bioRxiv - Microbiology 2020Quote: ... Phage stocks were treated with 18 U DNase I (New England Biolabs, M0303) and 0.1mg RNase A (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... the stock was diluted in water with 0.5X CutSmart Buffer (New England Biolabs) and 0.1% filter-sterilized phenol red ...
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bioRxiv - Microbiology 2020Quote: ... The Flag-tagged spike gene was ligated to the linearized vector using the T4 DNA Ligase enzyme from NEB. The resulting plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ... and T478K of the Delta variant was spliced into the spike expression vector by HiFi DNA assembler cloning (NEB). The plasmid was prepared and verified as described above.
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bioRxiv - Microbiology 2021Quote: ... without codon optimization and was inserted into pcDNA 3.1 to g et pcDNA 3.1-SARS-CoV-2-Spike using NEBuilder® HiFi DNA Assembly Master Mix (NEB) a ccording to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... The C-terminal Flag tag was introduced into pcDNA3.1-Spike-WT thanks to NEBuilder® HiFi DNA Assembly (NEB). The Flag tag was added during amplification of Spike-WT from pcDNA3.1-Spike-WT with the primers ...
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bioRxiv - Cell Biology 2023Quote: ... 250 μL of 2 mg mL-1 Y289L GalT,46 50 μL of 500 kU mL-1 PNGase F (New England Biolabs, 5 U μg-1 of protein), and 62.5 μL of Lambda Protein Phosphatase (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... Clean deletions were verified via PCR using Quick-Load Taq Master Mix (New England Biolabs) or Q5 High Fidelity Master Mix ...
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bioRxiv - Genetics 2022Quote: ... The supernatant (~12 ml) was added to 1 ml HEGX washed chitin magnetic resin (NEB) and left to bind for 1 h at 4°C ...
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bioRxiv - Genomics 2021Quote: ... PCR reactions were cleaned up using 0.9X NEBNext Sample purification beads (E7137AA, New England Biolabs) and eluted in 25μL of 0.1X TE ...
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bioRxiv - Microbiology 2020Quote: ... a single-pot reaction was set up containing T4 ligase (10 000U) and buffer (NEB), SapI (NEB ...
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bioRxiv - Synthetic Biology 2019Quote: ... mRNA pull-up (polyA selection) was performed using Magnetic Isolation Module (NEB, cat no. E7490) and sheared before ligation of library primers ...
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bioRxiv - Molecular Biology 2020Quote: ... This TRE-LSL plasmid was then opened up using restriction enzymes XbaI and EcroI (NEB). After which the remaining three inserts ...
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bioRxiv - Immunology 2021Quote: The DNA sequences of B.1.351 and B.1.617 SARS-CoV-2 spikes for the mRNA transcription and pseudovirus assay were synthesized as gBlocks (IDT) and cloned by Gibson Assembly (NEB) into pcDNA3.1 plasmids ...
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bioRxiv - Biochemistry 2021Quote: 20 μg of SARS-CoV-2 Spike trimer was deglycosylated by incubating with 2.5 μL of PNGase F (NEB, SG) under native condition at 37 °C for 4 h ...
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bioRxiv - Microbiology 2021Quote: ... The amino acid deletions in the full-length SARS-CoV-2 Spike expressor were generated using the Q5 site-directed mutagenesis kit (NEB). The presence of the desired mutations was determined by automated DNA sequencing ...
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bioRxiv - Microbiology 2022Quote: ... The D614G spike plasmid was generated by introducing the mutation into the Wuhan reference strain via Q5 site-directed mutagenesis (NEB). The T95I ...
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bioRxiv - Microbiology 2022Quote: ... Mutations were introduced into the phCMV-Spike plasmids using the mutagenic primers and the Q5 site directed mutagenesis kit (NEB). In addition ...
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bioRxiv - Synthetic Biology 2020Quote: ... 0.5 µL 1 mg/mL bovine serum albumin (NEB), 0.25 µL T4 DNA ligase at 400 U/µL (NEB) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 μL of 5000 units/mL RNaseH (NEB, M0297L) was used per µg of DNA and incubated for 48 hours at 37°C ...
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bioRxiv - Genomics 2021Quote: ... 1 μL of Endonuclease IV (10,000 U/ml, NEB) was added to the reaction ...
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bioRxiv - Microbiology 2020Quote: ... 1 µL of T4 DNA ligase (400,000U/mL, NEB) and 2 µL ddH2O ...
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bioRxiv - Cancer Biology 2020Quote: ... 0.02 mg/ml DNase 1 (New England Biolabs, USA), 0.2 mg/ml dispase (Merck ...
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bioRxiv - Biochemistry 2022Quote: ... 1:100 (v/v) BSA (NEB Inc. 20mg/ml) was included ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μl of 5000 units/ml RNaseH (NEB, M0297L) was used per µg of DNA and incubated for 48 hours at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... once with 1 mL T4 ligase reaction buffer (NEB) and then resuspended in 50 μL of end-repair reaction mix (0.4 mM of dNTPs ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL Proteinase K (800 units/ml, NEB, # P8107S) was diluted in 20 μL H2O and added to the thermocycled emulsions ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 μL NEB Taq polymerase (5000U/mL) (BioLabs, # M0267S), 1 μL MgCl2 (25 mM ...
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bioRxiv - Genomics 2023Quote: ... 1% (v/v) BSA (20 mg/ml, NEB, B9000S) + 5μL of hash oligo (10 uM ...
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bioRxiv - Biochemistry 2023Quote: ... 1 U/mL murine RNase inhibitor (New England Biolabs), 60 nM linearized plasmid template ...
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bioRxiv - Molecular Biology 2023Quote: ... 1% SDS and 10 U/ml Proteinase K (NEB), followed by an 18-hour incubation at 56 °C with vigorous shaking ...
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bioRxiv - Neuroscience 2023Quote: ... 1% (v/v) BSA (20 mg/ml, NEB, B9000S) + 5μL of hash oligo (10 uM ...
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bioRxiv - Molecular Biology 2020Quote: ... Then 1.5 µl of 10 mM stock of the m7GpppA (S1405L, New England Biolabs) was added to crystal slurry and the mix was loaded on a 60 µm grid made from nylon (NY6004700 ...
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bioRxiv - Microbiology 2021Quote: ... Virus stocks were recovered following linearization of infectious clone plasmids using NotI-HF (NEB), in vitro transcription ...
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bioRxiv - Microbiology 2023Quote: ... Xho1 digestion of the plasmid stock was followed by phosphatase treatment using rSAP (NEB) to dephosphorylate the cut ends of the plasmid ...
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bioRxiv - Molecular Biology 2021Quote: ... Experiments comparing the impact of DNase I and RNase A on the migration of AbmR by SEC were completed by adding approximately 200 ug of purified AbmR 39S complexes to 20 units of DNase I (New England Biolabs) or 50 ug/ml of protease-free RNase A (Thermo Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Approximately 8 ug of <200nt RNA per sample was mixed to an equal volume of 2x loading dye (NEB #B0363A) and incubated at 70°C for 5min ...
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bioRxiv - Cell Biology 2019Quote: ... 95°C for 10 minutes and equal amounts of lysates (180 ug) were treated with either 1uL of EndoH (NEB), PNGase F (NEB) ...
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bioRxiv - Molecular Biology 2022Quote: ... Poly(A)+ RNA transcript was isolated from 1 ug purified total RNA (RNA integrity number >9.0) with NEBNext poly(A) mRNA magnetic isolation module (New England Biolabs #E7490). RNA-seq libraries were prepared with NEBNext Ultra Directional RNA library preparation kit for Illumina (New England Biolabs #E7420S ...
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bioRxiv - Molecular Biology 2023Quote: ... sgRNA amplicons were generated by PCR of 2 ug genomic DNA per 100 ul reaction with Q5 2X Hot Start Master Mix (M0494L, NEB) and enough reactions to maintain 1000x screening sgRNA representation ...
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bioRxiv - Microbiology 2022Quote: ... Clean RNA was first rRNA depleted using the NEBNext rRNA depletion kit (Human/Mouse/Rat) (NEB) before being prepared for sequencing using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Microbiology 2020Quote: ... stained with a mAb cocktail composed of SARS-CoV-2 spike (Creative-Bios; 2BCE5) and SARS-CoV-2 nucleoprotein (Creative-Biolabs; NP1C7C7) followed by anti-Mouse IgG-HRP (Abcam ab6823 ...
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bioRxiv - Immunology 2022Quote: ... The spike plasmids were linearized by restriction enzymes and transcribed to mRNA by in vitro T7 RNA polymerase (NEB, Cat # E2060S) as previously described7,8.
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bioRxiv - Immunology 2021Quote: ... The D614G Spike was generated by introducing the corresponding mutation in the Wuhan reference strain using Q5® Site-Directed Mutagenesis Kit (NEB).
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bioRxiv - Developmental Biology 2021Quote: ... mRNA pull-up was performed using the Magnetic Isolation Module (New England BioLabs, Catalog no. E7490). All 16 libraries were mixed at equal molarity in a single tube ...