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Citations for New England Biolabs :
351 - 400 of 3826 citations for 7 11 Dimethyldodeca 4 6 10 trien 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... Templates were then eliminated by one-hour Dpn-I (New England Biolabs) restriction digestion at 37°C and the PCR products of mutants were ligated using standard protocols to obtain circular Gateway entry clones carrying desired mutations ...
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bioRxiv - Genomics 2021Quote: ... and Luna Universal One-Step RT qPCR Master Mix kit (NEB E3005L) and Tween-20 (Sigma-Aldrich P1379) ...
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bioRxiv - Molecular Biology 2022Quote: ... Templates were then eliminated by one-hour Dpn-I (New England Biolabs) restriction digestion at 37°C and the PCR products of mutants were inserted into EcoRV digested C-terminal GFP expression vectors using Gibson assembly ...
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bioRxiv - Microbiology 2022Quote: ... reverse transcribed and amplified with OneTaq one-step RT-PCR kit (NEB) for verification by Sanger sequencing.
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bioRxiv - Microbiology 2022Quote: ... or Luna Universal Probe One-Step RT-qPCR Kit (New England Biolabs) or LightCycler® Multiplex RNA Virus Master (Roche ...
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bioRxiv - Synthetic Biology 2023Quote: ... Following one-hour outgrowth in 1 mL of SOC medium (NEB #B9020S) at 37°C ...
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bioRxiv - Synthetic Biology 2023Quote: ... Following one-hour outgrowth in 500 µL of SOC medium (NEB #B9020S) at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... One unit of β-agarase I (New England Biolabs; Ipswich, Massachusetts, USA) and 10 µL of 10x reaction buffer was added to 100 μL of cell suspension and incubated at 42°C for 1.5 h ...
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bioRxiv - Biophysics 2023Quote: ... was prepared by one-step cloning using NEBuilder HiFi reaction (NEB # E5520S) into plasmid pIA14636 linearized with HindIII (NEB # R3104S ...
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bioRxiv - Biochemistry 2023Quote: ... Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB M3019) or the Luna Universal Probe One-Step RT-qPCR Kit (NEB E3006 ...
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bioRxiv - Biochemistry 2023Quote: ... and the Luna Universal Probe One-Step RT-qPCR Kit (NEB E3006) were used to detect the N1 (FAM ...
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bioRxiv - Biochemistry 2023Quote: ... or the Luna Universal Probe One-Step RT-qPCR Kit (NEB E3006) following the standard NEB protocol or SalivaDirect RT-qPCR amplification protocol as indicated ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluted chromatin was digested for one hour in RNaseIII (NEB, M0245), purified with MinElute PCR purification kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... One µg pJC53.2 was digested with Ahd I (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Molecular Biology 2023Quote: ... One µg pJC53.2 was digested with Ahd I (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Neuroscience 2023Quote: ... The purified DNA fragments were ligated into one of four BsmBI (NEB) digested TALEN expression vectors encoding one of four possible RVDs using Quick T4 DNA ligase ...
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bioRxiv - Microbiology 2024Quote: ... and Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... using the Luna Universal One-Step RT-qPCR kit (New England Biolabs) with the following primers:
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bioRxiv - Cell Biology 2023Quote: ... cells were resuspended in 50 mL buffer 3 (20mM KPi pH 7.4, 1.2M Sorbitol, 10 mM ribonucleoside vanadyl complex (NEB S1402S, pre-warmed at 65 °C), 0.08mg/ml Zymolyase-20T (Amsbio 326921) ...
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bioRxiv - Genomics 2022Quote: ... A restriction digestion mix containing 7 µl of 10X NEB CutSmart Buffer (NEB #B7204), 4.5 µl of NlaIII (NEB #R0125) ...
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bioRxiv - Genomics 2020Quote: ... A-tailing 3’ end was performed using Klenow Fragment (3’→5’ exo-) (New England Biolabs), and then TruSeq Adapters were ligated by Quick T4 DNA Ligase (New England Biolabs) ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by addition of 3’-A overhangs using Klenow Fragment 3’-5’ exo- (NEB, M0212S). After denaturation of DNA at 95°C for 3 min ...
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bioRxiv - Cell Biology 2021Quote: ... A-tailing 3’ end was performed using Klenow Fragment (3’→5’ exo-) (New England Biolabs), and then TruSeq Adapters were ligated by Quick T4 DNA Ligase (New England Biolabs) ...
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bioRxiv - Molecular Biology 2022Quote: ... using the random primer method (Random Primer 6, NEB S1230S ...
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bioRxiv - Neuroscience 2020Quote: ... comprising 6 mM 5’ cap analog (New England Biolabs), 7.5 mM adenosine triphosphate and 1.5 mM guanosine triphosphate (USB ...
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bioRxiv - Biochemistry 2019Quote: ... and terminated by adding 6× DNA loading buffer (NEB). For cleavage assay presented in Figs ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 4: 4°C hold) using NEB Next High-Fidelity master mix (NEB) in 25 µl reaction volume using RAD-Marker for/ RAD-Marker rev primers (25 nM each ...
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bioRxiv - Genomics 2022Quote: ... Proximity barcode ligations were carried out in 150 µl T4 ligation reaction mix (11 µL T4 ligase (NEB cat# M0437B-BM), 75 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... 40 µL of ChIP DNA or about 400 ng of Input DNA were added to 70 µL of blunting mix (11 µL 10X NEBuffer 2 (NEB, B7002S), 0.5 µL 10 mg/mL BSA ...
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bioRxiv - Genetics 2020Quote: ... PMO-induced alternative splicing of ush2a transcripts was analysed by PCR amplification using primers in zebrafish ush2a exon 11 using Q5 HF DNA polymerase (New England Biolabs, #M0491L). Primer sequences are provided in table S2 ...
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bioRxiv - Microbiology 2020Quote: ... Crude and purified proteins were further analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and its molecular weight (Broad range 11 to 245 kDa, BioLabs, England) was determined [14].
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bioRxiv - Genomics 2021Quote: ... Tagmented DNA fragments were amplified by adding 12 µL PCR master mix composed of 11 µL Q5 High-Fidelity 2x Master Mix (New England Biolabs, #M0492) and 0.5 µL each of 10 mM Nextera i5 and i7 index primers ...
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bioRxiv - Cell Biology 2021Quote: ... on a Mini Gel Tank (Thermo #A25977) using MES SDS Running Buffer (Thermo #B0002) alongside broad range markers (11-245KDa, NEB #P7712S). Each lane contained 20 μg of protein ...
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bioRxiv - Immunology 2022Quote: ... Caspase-11 catalytic (C254A) and cleavage (D285A) mutants were generated by site-directed mutagenesis (Q5 SDM Kit, New England BioLabs; #E0554S) of the wild-type parent vector according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... which adds homology arms and YL_randBCs_R1_3’ which adds random 11 nt barcodes and downstream homology arms (NEB Q5 polymerase Tm=70C). The PCR product was pooled and cleaned using the Monarch PCR and DNA kit ...
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bioRxiv - Cell Biology 2020Quote: ... Oct-4 (NEB, D7O5Z), Sox2 (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 ml ScaI (BioLabs), followed by 3 h of incubation with a fresh portion ...
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bioRxiv - Cancer Biology 2021Quote: ... 3’ end filling and dA tailing was performed by Klenow Fragment (3’>5’ exonuclease deficient; NEB). Libraries were prepared by ligation of NEBNext adapters and indexed i7 primers (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... The 3’ linker (5′-rAppGTGTCAGTCACTTCCAGCGG-3’, Dharmacon) was added using T4 RNA Ligase 2 (NEB, M0242S), followed by the PNK (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... Pu TKamplicon (5’-CTGTTTTCATTCTGCCTTTTGACCATAGAGCCCACCGCATCC-3’ and 5’-GCCAACAAAGAAAGCCTCACTACC GGGTAGGGGAGGCG -3) and Gibson Assembly master mix (NEB) following the manufacturer’s guidelines.
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bioRxiv - Molecular Biology 2020Quote: ... and one half treated with lambda protein phosphatase enzyme (New England Biolabs, P0753S) to the final concentration of 2.25 U/μl for 30 minutes at 30C ...
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bioRxiv - Genomics 2019Quote: ... After two washes in binding buffer and one wash in ligation buffer (NEB), PE adapters (Illumina ...
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bioRxiv - Immunology 2021Quote: ... Luna Universal Probe One-step RT-qPCR kit (New England Biolabs, Ipswich, MA) was used to amplify the envelope (E ...
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bioRxiv - Bioengineering 2021Quote: ... Amplification was performed in one of the following buffers – 1X Isothermal buffer (NEB) (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... One μL of T7 endonuclease I (no. M0302S; New England Biolabs, Ipswich, MA) was added to the mix and incubated for 15 min at 37°C ...
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bioRxiv - Plant Biology 2022Quote: ... One μg of total RNA was treated with DNase I (New England Biolabs) prior to the first-strand cDNA synthesis to eliminate genomic DNA contamination ...
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bioRxiv - Cancer Biology 2022Quote: ... One microgram of RNA was ribodepleted using NEBNext rRNA Depletion Kit (NEB #E6310). Ribodepleted RNA was fragmented ...
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bioRxiv - Molecular Biology 2021Quote: ... The Luna Universal Probe One-Step RT-qPCR Kit (Cat. No. E3006S, NEB) was used as the relevant master mix ...
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bioRxiv - Microbiology 2021Quote: ... The RT-qPCRs with Luna Universal One-Step RT-qPCR kit (NEB, USA) were performed according to the protocol of the manufacturer ...
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bioRxiv - Genomics 2022Quote: ... RT-qPCR was performed using Luna Universal One-Step RT-qPCR kit (NEB) using the following primers ...