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Citations for New England Biolabs :
3751 - 3800 of 9843 citations for Swine IL 4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... The cleaved DNAs were subjected to A-tailing using NEBNext Ultra II End Prep kit (NEB) and followed by ligation of an annealed hairpin adaptor (5’-phos-CGGTGGACCGATGATC/ideoxyU/ATCGGTCCACCG*T-3’ ...
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bioRxiv - Genetics 2022Quote: ... Sequencing libraries were prepared using the NEBNext Ultra™ II PCR-free kit (New England Biolabs). In short ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized using a ProtoScript First Strand cDNA synthesis kit (New England Biolabs, MA, E6300S) using PolyDT primers ...
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bioRxiv - Physiology 2022Quote: ... All mutagenesis was done using the Q5® Site-Directed Mutagenesis Kit from NEB (Cat # E0554S). The sequence of oligonucleotides used for this mutagenesis reaction is available on request ...
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bioRxiv - Molecular Biology 2022Quote: ... gel purified and in vitro transcribed using the HiScribe T7 high yield RNA synthesis kit (NEB) in a 20 μl reaction for 10 h at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... These were synthesised by IDT and converted into sgRNAs using the EnGen Cas9 sgRNA kit (NEB) according to instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Sequencing library preparation was performed using the NEBNext Ultra II Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... we introduced a 6-bp mutation using the Q5 site-directed mutagenesis kit (New England Biolabs). The pUAST-attB constructs were inserted into either attP40 or attP86Fb (for Piezo constructs ...
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bioRxiv - Neuroscience 2021Quote: RNA was collected from cultured granule cells using the Monarch® Total RNA Miniprep Kit (NEB). Then cDNA was reverse transcribed using SuperScript™ IV First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: Library amplification: Amplification was performed with components of the NEBNext Ultra II DNA kit (NEB E7645S) and a NEBNext Multiplex Oligos set (e.g ...
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bioRxiv - Molecular Biology 2020Quote: ... or the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, USA) was used to transcribe the templates ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA-seq experiments were performed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Genetics 2020Quote: ... cDNA was generated using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB (UK Ltd.), Hitchen ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were prepared for sequencing using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) with NEBNext Multiplex Oligos (New England Biolabs) ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were gel purified with Monarch® DNA Gel Extraction Kit (T1020S, New England BioLabs) according to manufacturer’s instructions and sent for NGS at the Gurdon Institute NGS core using an Illumina HiSeq 1500.
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bioRxiv - Genomics 2020Quote: ... The library was then prepared using the NEBNext Ultra II direction Library preparation kit (NEB, E7760), and sample cleanups were performed using the Omega NGS Total Pure Mag Beads (Omega ...
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bioRxiv - Genomics 2019Quote: MNase sequencing libraries were prepared using NEBNext Ultra DNA library Prep Kit for Illumina (NEB #E7370S), using 30ng of input mononucleosomal DNA from each digestion level and time point ...
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were constructed using NEBNext UltraTM RNA Library Prep Kit for Illumina (#E7530L, NEB, USA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
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bioRxiv - Immunology 2021Quote: ... In-vitro-transcription was carried out using the Hi-Scribe RNA transcription kit (New England BioLabs). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA samples (800ng) were depleted of rRNA using the NEBNext rRNA Depletion kit (New England Biolabs). RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... mRNA-seq libraries were prepared using the NEBNext ultra (or ultra II) Directional RNA kit (NEB) with the NEBNext Poly(A ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... Genomic DNA (gDNA) was extracted using the Monarch Genomic DNA Purification Kit (New England BioLabs, #T3010S) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Sequencing libraries were prepared without rRNA depletion using NEBNext Ultra II Directional RNA Library kit (NEB) with the following modifications ...
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bioRxiv - Microbiology 2019Quote: ... Libraries were created using the NEBNext Ultra DNA Library Prep Kit (New England Biolabs, Ipswich, MA) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... The cDNA library was constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) and 3 μg RNA per sample ...
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bioRxiv - Molecular Biology 2019Quote: ... The Cap 1 25mer RNA was purified using Monarch RNA Cleanup kit (50 μg capacity, NEB). Complete methylation was verified by digestion of RNA with the Nucleoside Digestion Mix (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 μg of linearised DNA was then used in a HiScribe T7 synthesis kit (NEB), before DNase treatment and purification using a PureLink RNA mini kit (Thermo Fisher).
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bioRxiv - Genomics 2019Quote: ... Standard indexed Illumina libraries were prepared using the NEBNext DNA Library Prep kit (New England BioLabs), followed by amplification using KAPA HiFI DNA polymerase (KAPA Biosystems) ...
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bioRxiv - Microbiology 2019Quote: ... The PCR products were then individually ligated using a KLD Enzyme reaction kit (New England Biolabs) for 3 hours and subsequently transformed into ElectroMAX DH10B E ...
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bioRxiv - Systems Biology 2019Quote: ... RT-qPCR was performed with the Luna Universal One-Step RT-qPCR Kit (New England Biolabs). 1 μl of 10-fold diluted RNA was added to 4 μl of rtPCR mix and subjected to a reverse transcription step at 55°C and 45 cycles of PCR (10 seconds at 95°C and 30 seconds at 60°C) ...
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bioRxiv - Cancer Biology 2020Quote: ... ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Preparation Kit (NEB, #E7645). Libraries were analyzed for fragment size using the Bioanalyzer High-Sensitivity DNA Kit (Agilent ...
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bioRxiv - Zoology 2020Quote: ... we prepared PCR-free libraries using NEBNext Ultra II DNA library prep kit (New England Biolabs), but with TruSeq DNA Single Indexes (Set B ...
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bioRxiv - Microbiology 2019Quote: ... The Illumina sequencing library were prepared with the NEBNext® Ultra DNA Library Prep kit (NEB) by selecting 400-bp fragments ...
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bioRxiv - Developmental Biology 2019Quote: Pceh-23_L::acy-2 fragment(anti-sense) was generated with a Gibson assembly cloning kit (NEB) by assembly of the following two DNA fragments ...
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bioRxiv - Cancer Biology 2020Quote: ... NEB Next Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) was used for the construction of sequencing libraries ...
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bioRxiv - Developmental Biology 2019Quote: ... before final assembly with pJET1.2 vector using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). Small Guide encoding plasmids for CRISPR were generated by an overlap extension PCR method performed on template pML2840.
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bioRxiv - Developmental Biology 2020Quote: ... Dppa3 and mutant Dppa3 (R107E) mRNA were synthesized using HiScribe T7 ARCA mRNA kit (NEB, E2060S), and purified using RNeasy mini kit (QIAGEN ...
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bioRxiv - Molecular Biology 2019Quote: ... Associated DNA was then purified by extraction using Monarch PCR & DNA Cleanup Kit (New England Biolabs). Immunoprecipitated DNA and total input were used as a template for real-time qPCR ...
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bioRxiv - Cancer Biology 2019Quote: ... and converted into libraries using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs).
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bioRxiv - Synthetic Biology 2019Quote: ... and subsequently cloned in to the pMini-T plasmid using the PCR Cloning Kit (NEB #E1202). Plasmids were extracted from E.coli JM109 transformed cells using the QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Immunology 2019Quote: ... and subjected to in vitro transcription (IVT) using HiScribe T7 High Yield RNA Synthesis Kit (NEB), then treated with ExoSAP-IT (Affymetrix 78200) ...
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bioRxiv - Microbiology 2020Quote: ... 1µL Random Primer Mix (ProtoScript II First Strand cDNA Synthesis Kit, New England Biolabs, https://www.neb.ca) was added to 7 µL extracted RNA and denatured on SimpliAmp thermal cycler (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... The MyoXIa-CCT bait was mutagenized with the Q5 Site-Directed Mutagenesis Kit (New England BioLabs) to create the V1418R and V1422R mutants ...
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bioRxiv - Microbiology 2020Quote: ... library preparation through Next® Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs) and HiSeq 2500 paired-end 150 bp sequencing.
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bioRxiv - Cancer Biology 2019Quote: ... After determining accurate concentrations by quantitative PCR using the NEBnext library quant kit for Illumina (NEB), pooled libraries were sequenced on an Illumina HiSeq 2500 platform using a 50 bp single read on a high output standard v4 flow cell with a 15-20% PhiX spike-in ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep kit (NEB). To quantitate the consistency of spike-in cell mixing for each individual sample ...
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bioRxiv - Developmental Biology 2020Quote: ... The sequencing-ready cDNA libraries were quantified with the NEBNext library quantification kit for Illumina (NEB). mRNA-seq libraries were sequenced with single-end 50 at ≈20 million reads per sample with biological triplicates ...
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bioRxiv - Microbiology 2020Quote: ... Gibson assembly was performed using NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs Inc.) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2019Quote: ... and subsequently processed with the "NEBNext Ultra II DNA Library Prep Kit for Illumina" (NEB #E7645). Briefly ...