Labshake search
Citations for New England Biolabs :
3651 - 3700 of 9843 citations for Swine IL 4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... The R36K variant was generated with the Q5® Site-Directed Mutagenesis Kit (NEB, # E0554S) from the pERLIN2-3xFlag using the manufacturer’s protocol (primers sequence ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries were prepared using the NEBNext® UltraTM II Library Prep Kit (New England Biolabs). Paired-end 150 sequencing was performed using the NextSeq 500 platform (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645) and analyzed with either 2100 Bioanalyzer or TapeStation systems (Agilent) ...
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bioRxiv - Molecular Biology 2024Quote: All constructs were cloned using the NEBuilder HiFi DNA Assembly kit (New England Biolabs E2621) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were prepared using the Directional RNA Library Prep Kit (E7760S, New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the Monarch® HMW DNA Extraction kit for Tissue (New England Biolabs, Ipswich, MA, USA) was used to improve DNA fragment length ...
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bioRxiv - Microbiology 2023Quote: ... Nef deletion Reverse: 5’-AGATCTACAGCTGCCTTGTAAGTCATTGG-3’) using Q5® Site-Directed Mutagenesis Kit (NEB #E0554S).
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were generated using the NEBNext Ultra RNA library prep kit for Illumina (NEB), and sequencing was performed on an Illumina NovaSeq 6000 platform ...
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bioRxiv - Microbiology 2023Quote: cDNA synthesis on all RNA samples was carried out using LunaScript RT SuperMix Kit (NEB) with 16 μl template RNA in 20 μl reactions according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was done with 100ng of RNA with the Luna RT-qPCR kit (NEB) using primers targeting beta actin as a control (Table S2) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was then prepared for sequencing with the NEBNext Small RNA library Prep kit (NEB). Sequencing libraries were visualized using an Agilent 2200 Tapestation and sequencing was done using the Novaseq 6000 SP300 cartridge (Illumina).
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bioRxiv - Microbiology 2023Quote: ... Libraries were prepared using NEBNext® Ultra™ Directional RNA Library Prep Kit (NEB, E7420) and amplified by PCR with KAPA HiFi HotStart Ready Mix (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were performed with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) according to the published protocol50 ...
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bioRxiv - Molecular Biology 2023Quote: HLTF’s cDNA on the pcDNA3.1(+) backbone54 was mutated using Q5 Site-Directed Mutagenesis kit (NEB) to generate the C760S and R890Q mutant ...
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bioRxiv - Molecular Biology 2023Quote: ... using the High Sensitivity DNA Assay and NEBNext Library Quant Kit for Illumina (NEB, E7630). Finally ...
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bioRxiv - Genomics 2023Quote: ... and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The mSA S27A mutation was created by site-directed mutagenesis using commercially-available kits (NEB). 4NBX.B_C103 and 2X89.A_CCAA_C57 sequences were directly ordered from IDT ...
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bioRxiv - Genetics 2023Quote: RNA sequencing library preparation used the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB), following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The FBXO22-C326A mutant plasmids are generated via Q5 Site-Directed Mutagenesis Kit (NEB E0552) based on the wildtype FBXO22-HaloTag plasmid.
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bioRxiv - Neuroscience 2023Quote: ... RNA was isolated using the Monarch® Total RNA Miniprep Kit (#T2010S; New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and cDNA was generated using Protoscript II First Strand cDNA Synthesis Kit (New England Biolabs). SARS-CoV-2 was detected by PCR using the 2019-nCoV RUO Kit (IDTDNA ...
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bioRxiv - Plant Biology 2023Quote: ... First-strand complementary cDNA was synthesized with LunaScript™ RT SuperMix Kits (New England Biolabs). Quantitative PCR was performed with a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... Ribosomal RNA was removed using NEBNext® rRNA Depletion Kit (Human/Mouse/Rat; NEB, E6310X). rRNA-depleted RNA was converted to a library using NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... Mutations on mEGFP-ATP7B were prepared following Q5® Site-Directed Mutagenesis Kit (NEB #E0554) protocol ...
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bioRxiv - Microbiology 2023Quote: ... The 3 fragments were ligated using the Gibson Assembly® Cloning Kit (New England Biolabs). The created pEXG2::ΔfahA and pEXG2::ΔpanC were transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB #E7645S/L) was used for library preparation and libraries were sequenced on Illumina MiSeq or NextSeq 2000 platform and more than 1.25M read pairs of 150bp length were generated per sample i.e ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: ... Single point mutant plasmids were constructed using Q5® site-directed mutagenesis kit (NEB, E0554S) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using a NEBNext Ultra II DNA library prep kit (NEB, Cat# E7645S) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted using the Monarch Genomic DNA Extraction Kit (New England Biolabs, Massachusetts, USA) before sequencing with MinION (Oxford Nanopore Technology ...
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bioRxiv - Cell Biology 2023Quote: ... and ∼250 bp fragments were gel extracted for cloning using a PCR Cloning Kit (NEB). The resulting plasmid was purified by Midiprep (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... RNAs were transcribed using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs). Reactions were set up as per manufacturer’s instructions with the addition of 4 units of Murine RNase Inhibitor (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... coli C2566 genomic DNA was purified using Monarch Genomic DNA Purification Kit (NEB Ipswich, MA), GM12878 genomic DNA was obtained from Coriell Cell Repositories (Camden ...
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bioRxiv - Immunology 2024Quote: ... Libraries were prepared using the NEBNext Ultra DNA Library Prep kit (E7645, New England Biolabs).
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bioRxiv - Biophysics 2024Quote: ... The purified dsDNA templates were transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... with the exception of KLF2 which was performed using the Q5 High-Fidelity kit (NEB). For the 11q deletion variant (P3) ...
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bioRxiv - Biophysics 2024Quote: The NEB Q5® Site-Directed Mutagenesis Kit (part number: E0554S, New England Biolabs Inc.) was used to perform the mutation responsible for mEGFP following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... mRNA was transcribed with HiScribe T7 mRNA Kit with CleanCap Reagent AG (New England Biolabs) according to manufacturer’s protocol with 1 µg of amplified template ...
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bioRxiv - Developmental Biology 2024Quote: ... in combination with NEBNext Ultra II directional RNA library prep kit for Illumina (NEB #E7765) following manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... mRNA was synthesized from linearized plasmid using HiScribe T7 high yield RNA synthesis kit (NEB), then capped with m7G (5’ ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries for sequencing were constructed with the NEB Next Ultra RNA Library Prep Kit (NEB) for Illumina ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted using the Monarch Total RNA Miniprep Kit (New England BioLabs #T2010S) and submitted to Azenta Life Sciences for library preparation and Illumina sequencing.
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bioRxiv - Microbiology 2024Quote: ... quantified the two pools using an NEBNext Library Quant Kit for Illumina (New England Biolabs), and determined average fragment size and adaptor dimer removal on a Tapestation 4200 (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... The PCR product was purified using the Monarch PCR and DNA cleanup kit (T1030S, NEB) and dsRNA was synthesised using the T7 RiboMAX express system (P1700 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and purified the rest of the digestion product with Monarch Nucleic Acid Purification Kit (NEB). We quantified the amount of purified DNA using the Qubit dsDNA HS Assay (Thermofisher ...
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bioRxiv - Cell Biology 2024Quote: ... The DNAse-digested RNA was purified using the Monarch RNA Cleanup kit (New England Biolabs) as recommended by the manufacturer and used as template for cDNA synthesis and SYBR Green-based qPCR (see quantitative real-time PCR (RT-qPCR ...
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bioRxiv - Microbiology 2024Quote: ... Additional mutations/deletions were introduced in gp140-T/F07 using site directed mutagenesis kit (NEB). The sequences of the recombinant clones were verified through sequencing (Retrogen ...
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bioRxiv - Synthetic Biology 2024Quote: ... Amplification products were gel extracted using the Monarch® DNA Gel Extraction Kit (NEB T1020).
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bioRxiv - Bioengineering 2024Quote: ... PCR reactions were purified using the Monarch DNA PCR Cleanup Kit (New England Biolabs; T1030). For Gibson assembly of constructs ...
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bioRxiv - Cell Biology 2024Quote: ... a NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (NEB, E7645S) was used according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (NEB; E7645) according to the manufacturer’s protocol ...