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Citations for New England Biolabs :
3551 - 3600 of 9843 citations for Swine IL 4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were prepared using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs). The quality of the libraries was assessed using the High Sensitivity DNA kit (Agilent ...
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bioRxiv - Genetics 2023Quote: ... The immunoprecipitated DNA was purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs). The libraries for Illumina sequencing were constructed using the ThruPLEX DNA-Seq Kit (Clontech ...
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bioRxiv - Genomics 2023Quote: ... and DNA was immediately isolated using the Monarch Genomic DNA Purification Kit (NEB, Cat # T3010S), with the modification that elution was carried out with 87.5 μL 25 mM K3BO3 solution (pH 7.0).
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bioRxiv - Genetics 2023Quote: ... and were quantified with the NEBNext Library Quant Kit for Illumina (New England BioLabs E7630L). Libraries were pooled to a final concentration of 4 nM and submitted to the Johns Hopkins Genomics Research Core Facility for high-throughput sequencing on the Illumina NovaSeq 6000 platform ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was synthesized using ProtoScript® II First Strand cDNA Synthesis Kit (New England BioLabs). gli1 and ptch1 mRNA expression in the cells exposed to SAG were measured by qRT-PCR using Roche the LightCycler® 480 instrument ...
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bioRxiv - Genomics 2023Quote: ... The PCR product was gel purified using the Monarch DNA Gel Extraction Kit (NEB T2010) and used for IVT reaction using the NEB HiScribe T7 ARCA mRNA Kit (E2065S).
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bioRxiv - Microbiology 2023Quote: ... followed by purification using the Monarch Gel Extraction Kit (New England Biolabs, catalog no. T1020L).
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified using Monarch PCR and DNA Cleanup kit (NEB, Ipswich, MA). DNA concentration was quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Resulting Fluc mRNA was purified twice by Monarch RNA cleanup kit (T2040S, NEB, Ipwsich, MA) to ensure that unincorporated nucleotides were completely removed ...
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bioRxiv - Microbiology 2023Quote: ... Gibson cloning was performed according to the protocol of the Gibson Assembly kit (NEB, E5520). To construct the Sch9-3E-NAT mutant and the matching Sch9-WT-NAT control strains ...
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bioRxiv - Molecular Biology 2022Quote: The NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs, Ipswich, Massachusetts, USA) was used to prepare 50 nt paired-end ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was first hybridized with the NEBNext rRNA Depletion kit v2 (NEB, cat# E7400) to diminish rRNA from the samples ...
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bioRxiv - Microbiology 2023Quote: ... rRNA was depleted by using the bacterial NEBNext rRNA Depletion Kit (New England BioLabs, E7850). Sequencing libraries were created by end repair and phosphorylation ...
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bioRxiv - Immunology 2023Quote: ... Reverse transcription (RT) was performed using ProtoScript First Strand cDNA Synthesis kit (New England Biolabs) to generate cDNAs ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was isolated using the Monarch® Total RNA Miniprep Kit (New England Biolabs) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... All PCR products were gel purified by using the Monarch DNA Gel Extraction Kit (NEB) and eluted in 20 μL nuclease-free water ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was isolated using the Monarch Total RNA Miniprep Kit (New England BioLabs, cat #T2010) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... assembled them using a NEBridge Golden Gate Assembly Kit (BsmBI-v2) (#E1602S, New England Biolabs), and then electroporated 1 μL of the reaction into electrocompetent NEB5alpha ...
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bioRxiv - Molecular Biology 2023Quote: ... and the desired products were agarose gel purified using a Monarch gel extraction kit (NEB) and sequenced on an Illumina HiSeq.
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bioRxiv - Neuroscience 2023Quote: ... and 7f variants were derived by site-directed mutagenesis (NEB Q5 Site-Directed Mutagenesis Kit) of the GCaMP6s sequence in pDSP23 to reproduce the mutations described for GCaMP6f (pDSP31 ...
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bioRxiv - Microbiology 2023Quote: ... RNA-seq library was constructed using NEBNext Ultra II RNA-library preparation kit (NEB, E7770S) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted with Monarch® HMW DNA Extraction Kit for Cells & Blood (NEB), digested with DpnI overnight at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... and DNase was removed using the Monarch RNA Cleanup Kit (50 µg; New England Biolabs). LunaScript RT SuperMix Kit (New England Biolabs ...
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bioRxiv - Molecular Biology 2023Quote: ... the NEBuilder HiFi Master Mix was used according to kit instructions (E5520S, New England Biolabs). All single integration plasmids were digested with PmeI before transformations and integrations were verified by PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... 2019) and were constructed using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and the NEBNext Ultra II Directional RNA library Prep Kit for Illumina (New England Biolabs). For library amplification 17 or 24 PCR cycles were used ...
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bioRxiv - Molecular Biology 2022Quote: ... Synthesis was performed with the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Ph.D.™-12 Phage Display Peptide Library Kit (New England BioLabs® Inc., MA, USA) was amplified as following the recommended protocols by the manufacturer ...
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bioRxiv - Genomics 2023Quote: ... Sequencing library was constructed using the NEB Ultra library preparation kit (New England Biolabs, Ipswitch).
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bioRxiv - Molecular Biology 2023Quote: ... The reverse-transcription (RT) reactions were performed with 2X LunaScript® RT SuperMix Kit (NEB) with 5 μL of RNA input in 10 μL total volume ...
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Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv - Molecular Biology 2023Quote: ... RNAs were in vitro transcribed with HiScribe T7 ARCA mRNA Kit with tailing (NEB, E2060S) following the manufacturer’s recommended protocol ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR was performed using a Luna ® Universal One-Step RT-qPCR kit (NEB) in a CFX96 qPCR thermocycler (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... hansenii NQ5 was isolated using the Monarch HMW DNA Extraction Kit for Tissue (NEB T3060S) per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... or HiScribe® T7 High Yield RNA Synthesis Kit (New England BioLabs, cat. no. E2040S) following the manufacturers’ protocols ...
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bioRxiv - Cell Biology 2023Quote: ... the MBNL1 binding site (TGCTGC) was deleted using Q5 Site Directed Mutagenesis kit (NEB E0554). 12.5 ng of pFlareA-Mapt Ex4 splicing reporter was used as template and mixed with 12.5 µl of Q5 Hot Start High-Fidelity 2X Master Mix ...
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bioRxiv - Biochemistry 2023Quote: ... For assessment of HAC1spliced mRNA the Luna Universal Probe One-Step RT-qPCR Kit (NEB) was used with 2 µl of a 50 ng/ µl RNA sample ...
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bioRxiv - Synthetic Biology 2023Quote: ... was created using the NEBNext Ultra II DNA library prep kit for Illumina (NEB #7103S) with index primer set 1 (NEB #7335S ...
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bioRxiv - Genomics 2023Quote: ... and libraries were prepared with a NEBNext Ultra II Library Prep Kit (New England Biolabs).
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bioRxiv - Genomics 2023Quote: ... (Sacramento, CA, USA) for library construction (NEBNext Ultra RNA Library Prep Kit, New England Biolabs) from 1 µg total RNA and Illumina sequencing ...
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bioRxiv - Evolutionary Biology 2023Quote: ... size selection and PCR enrichment using the NEBNext Ultra Directional RNA Library Prep Kit (NEB). PCR enrichment step was performed with NEBNext Multiplex Oligos for Illumina (Dual Index Primers ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA was extracted from subconfluent cells with the Monarch total RNA purification kit (NEB), and reverse transcribed with LunaScript RT SuperMix (NEB) ...
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bioRxiv - Cancer Biology 2023Quote: ... Libraries were prepared with NEBNext Ultra DNA Library Prep Kit for Illumina (New England Biolabs). Regions to sequence were selected with the SeqCap EZ Human Exome Library v3.0 (Roche Applied Science ...
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bioRxiv - Molecular Biology 2023Quote: ... the amplified oligo pool was gel- purified using a Monarch DNA Gel Extraction Kit (NEB). To generate the linearized vector for the light chain variant library ...
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bioRxiv - Molecular Biology 2023Quote: ... plasmids were edited via site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, NEB, Cat #E0554).
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bioRxiv - Neuroscience 2023Quote: ... using the NEBNext Ultra II RNA Library Prep with Purification Beads Kit (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... followed directly by using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB #E7760S) and NEBNext Multiplex Oligos for Illumina (NEB #E7600S ...
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bioRxiv - Synthetic Biology 2023Quote: ... IVT mRNA was produced using the HiScribe T7 High Yield RNA Synthesis Kit (NEB # E2040) along with 500 ng of linear template and 4 mM CleanCap AG Reagent (Trilink ...
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bioRxiv - Cell Biology 2023Quote: The libraries were constructed using NEBNext Ultra RNA Library Prep Kit for Illumina (E7530L, NEB). Sequencing was performed on an Illumina NovaSeq 6000 platform with 2D×D150 bp pair-end reads at Novogene ...
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bioRxiv - Plant Biology 2023Quote: ... The point mutant K665A SPY was generated via Q5 Site-Directed Mutagenesis Kit (NEB, E0552S). All plasmids were sequenced to ensure accuracy.
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bioRxiv - Physiology 2023Quote: ... Mutagenesis was done using the Q5® Site-Directed Mutagenesis Kit from NEB (Cat # E0554S). The sequence of oligonucleotides used for this mutagenesis reaction is available on request ...