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Citations for New England Biolabs :
3951 - 4000 of 9843 citations for Swine IL 4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were prepared with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, #E7420S). Sequencing was performed by LAFUGA on an Illumina Hiseq 1500 using 50 bp paired end run.
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bioRxiv - Molecular Biology 2020Quote: Library preparation was performed using the Ultra II DNA Library prep kit for Illumina (NEB, #E7645S) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2020Quote: ... and DNA assembly with NEBuilder kit were done according to the manufacturer recommendations (New England Biolabs). DNA sequences of constructs were confirmed by sequencing with the appropriate primers ...
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bioRxiv - Developmental Biology 2020Quote: ... The amplified products were cloned using the NEB PCR cloning kit (New England Biolabs, Ipswich, MA). Clones from each founder were Sanger sequenced using the NEB analysis primers to define indels.
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bioRxiv - Cell Biology 2020Quote: ... gel purified and ligated with pENTR (w158-1)-backbone by Quick Ligation kit (New England Biolabs) to create pENTR-GRET ...
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bioRxiv - Synthetic Biology 2019Quote: ... transcription templates were generated by PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs #E0553) of the plasmids to include a 5’ region upstream of the T7 promoter and a 3’ region ending with either 3WJdB or the T7 terminator with the primers listed in Supporting Data File 1 ...
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bioRxiv - Genomics 2020Quote: ... followed by DNA clean-up using the Monarch PCR & DNA Cleanup Kit (New England BioLabs T1030S). Library preparations were quantified using the using KAPA library quantification kit (Kapa Biosystems KK4824 ...
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bioRxiv - Microbiology 2020Quote: ... and PCR mixes using the Q5® High-Fidelity PCR Kit (New England Biolabs, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... The mutant was generated using the Q5 ® Site-Directed Mutagenesis kit (NEB, catalog number #E0554S) according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2020Quote: ... Library construction was done using NEBNext Ultra™ II DNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... and subject to library preparation using NEBNext® Ultra™ II DNA Library Preparation Kit (NEB). Libraries with barcodes were pooled and sequenced on the Illumina HiSeq 1500 platform ...
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bioRxiv - Molecular Biology 2020Quote: DNA libraries were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645) with index primers (NEB ...
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bioRxiv - Synthetic Biology 2020Quote: ... cDNA was synthesized from 500ng RNA using the Protoscript II First Strand cDNA synthesis Kit (NEB). 1ul of cDNA was amplified by PCR with primers that amplify about 200 bp surrounding the sites of interest using OneTaq PCR Mix (NEB) ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA libraries were generated from RNA using the NEBNext Ultra Directional RNA Library Preparation kit (NEB). Samples were sequenced by 75 bp paired-end sequencing using a NextSeq 500 (Illumina).
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina® (NEB, USA). The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina) ...
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bioRxiv - Synthetic Biology 2020Quote: ... cDNA was synthesized from 500ng RNA using the Protoscript II First Strand cDNA synthesis Kit (NEB). 1ul of cDNA was amplified by PCR with primers that amplify about 200 bp surrounding the sites of interest using OneTaq PCR Mix (NEB) ...
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bioRxiv - Genetics 2021Quote: ... DNA libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB# E7645), using Ampure XP beads for cleanup ...
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bioRxiv - Molecular Biology 2021Quote: IVT was carried out using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB E2050S) with the addition of RNAsin (Promega N2111) ...
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bioRxiv - Molecular Biology 2021Quote: ... We prepared cDNA from 1μg of extracted RNA using LunaScript® RT SuperMix Kit (NEB, #E3010). No Template and No Reverse Transcriptase controls (NTC and NRT ...
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bioRxiv - Microbiology 2020Quote: ... processed the extracted DNA with the NEBNext Microbiome DNA Enrichment Kit (New England Biolabs, Ipswich, MA), and quantified the resulting DNA using Picogreen (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: β1-tubulin wild type construct was generated by the gibson assembly (HiFi Kit, New England Biolabs) of a TubB1 sequence fragment synthesised as a gBlock by Integrated DNA Technologies (IDT ...
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bioRxiv - Synthetic Biology 2020Quote: ... GLuc activity in samples was measured using the Biolux Gaussia Luciferase Assay kit (NEB, Ipswich, MA). GLuc assay solution (50 μL ...
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bioRxiv - Genetics 2020Quote: ... We prepared the library using NEB Ultra II Directional RNA library kit for Illumina (NEB E7760) used dual index primers (from NEBNext Multiplex Oligos for Illumina ...
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bioRxiv - Plant Biology 2020Quote: ... mRNAs for PARE libraries were isolated using the NEB Magnetic mRNA Isolation Kit (New England Biolabs), custom adapters for selecting 5’-P mRNAs and primers from the TruSeq Small RNA Sample Preparation Kit (Illumina ...
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bioRxiv - Plant Biology 2020Quote: ... mRNAs for RNA libraries were isolated using the NEB Magnetic mRNA Isolation Kit (New England Biolabs). RNA libraries were produced using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... thlaspeos was extracted using the NEB Monarch Genomic DNA Purification Kit (New England Biolabs, Frankfurt, Germany).
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bioRxiv - Genomics 2019Quote: ... We inserted the minimal promoter and GFP using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB) as described above.
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bioRxiv - Physiology 2020Quote: ... Prior to stranded mRNA isolation library construction using NEBnext library kits (New England Biolabs, Evry, France), the quality of the RNA pool was assessed on a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Plant Biology 2019Quote: ... and libraries were prepared using NEBNext® Ultra™ DNA Library Prep Kit (New England Biolabs) followed by size selection (~320 bp ...
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bioRxiv - Biophysics 2019Quote: ... Mutants of skRLC and smRLC were obtained by Q5 site-directed mutagenesis kit (New England BioLabs) in pET3a vector ...
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bioRxiv - Genetics 2019Quote: ... were generated with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). Illumina stranded and paired-end (PE ...
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bioRxiv - Biochemistry 2019Quote: ... HSPA1 R469A and HSP8 R469A mutants was performed using Q5® Site-Directed Mutagenesis Kit (NEB), following manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... Reverse transcriptase was performed with the ProtoScript II First Strand cDNA synthesis kit (New England BioLabs) using 1µg of total RNA with random hexamer primers ...
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bioRxiv - Evolutionary Biology 2019Quote: Sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA), following manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... One-step qRT-PCR was performed using the Luna Universal One-Step RT-qPCR Kit (NEB) and 5 ng RNA per reaction in technical duplicate with a StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Genomics 2021Quote: ... ribosomal RNA depletion was performed using 1 μg RNA using NEBNext rRNA Depletion kit (NEB#6310). Concentration of final libraries was measured using Qubit 2.0 Fluorometer in combination with Qubit dsDNA HS Assay Kit (Invitrogen) ...
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bioRxiv - Genetics 2021Quote: ... Libraries were generated with the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs). Briefly ...
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bioRxiv - Genetics 2020Quote: The pVG344 plasmid was cloned using the NEBuilder HIFI DNA Assembly Cloning Kit (New England Biolabs) to assemble four amplified fragments ...
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bioRxiv - Genetics 2020Quote: ... without modification except for library preparation performed with NEBNext® Ultra kit (New England Biolabs®). For library preparation 600 ng of each genomic DNA were fragmented by sonication and purified to yield fragments of 150-200 bp ...
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bioRxiv - Genetics 2020Quote: ... Both cloning steps were performed with the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). The AarI-AarI fragment from pLdhGao23 was cloned into the AarI site of pScarless-dhGaoR ...
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bioRxiv - Cell Biology 2021Quote: ... mRNAs were isolated from total RNAs with Magnetic mRNA Isolation Kit (oligo(dT) beads) (NEB, E7490). First strand cDNAs were synthesized using random primers and second strand cDNAs were synthesized subsequently ...
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bioRxiv - Evolutionary Biology 2020Quote: ... All ligations to generate this construct were done with the Quick Ligation™ Kit (NEB, M2200S). The final construct was sequence validated ...
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bioRxiv - Evolutionary Biology 2020Quote: ... All ligations to generate this construct were done with the Quick Ligation™ Kit (NEB, M2200S) and the final construct was sequence validated ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The OdinTubulin mutation (H393D) was introduced using the Q5 Site Directed Mutagenesis Kit (New England BioLabs) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was subjected to rRNA depletion using the NEBNext rRNA Depletion Kit (New England Biolabs), according to the manufacturer’s protocol ...
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Plasma derived cell-free mitochondrial DNA originates mainly from circulating cell-free mitochondriabioRxiv - Genomics 2021Quote: ... DSP libraries were prepared with the NEB Next® Ultra™ II kit (New England Biolabs). SSP libraries were prepared with the Swift ACCEL-NGS® 1S PLUS kit (Swift Biosciences) ...
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bioRxiv - Genomics 2021Quote: ... Standard indexed Illumina libraries were prepared using the NEBNext DNA Library Prep kit (New England BioLabs), followed by amplification using KAPA HiFI DNA polymerase (KAPA Biosystems) ...
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bioRxiv - Genomics 2021Quote: ... UMI-4C sequencing libraries were generated using the NEBNext Ultra II library prep kit (NEB, E7645L). For each sample ...
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bioRxiv - Molecular Biology 2020Quote: ... The libraries were amplified and indexed according to the standard protocol (small RNA sequencing kit, NEB), except that PCR conditions were adjusted in order to prevent library over amplification ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB) and sequenced on the Illumina HiSeq 2500 platform ...