Labshake search
Citations for New England Biolabs :
3601 - 3650 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2022Quote: ... Total RNA was purified with the Monarch RNA Cleanup kit (New England Biolabs, Ipswich, MA, US).
-
bioRxiv - Molecular Biology 2022Quote: ... and genomic DNA was extracted by using a Monarch Genomic DNA Purification Kit (New England Biolabs). The thyA gene region was amplified from the genomic DNA (5 ng ...
-
bioRxiv - Molecular Biology 2022Quote: ... and genomic DNAs were extracted by using a Monarch Genomic DNA Purification Kit (New England Biolabs) according to the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2022Quote: ... DNA libraries were constructed using the NEBNext Ultra II FS DNA Library Prep kit (NEB, USA) and sequenced using the Illumina HiSeq X Ten platform.
-
bioRxiv - Microbiology 2022Quote: ... For the MksB hydrolysis mutant (MksBE1042Q) a site-directed mutagenesis (Q5 site-directed mutagenesis Kit, NEB) was performed using primer pair MG060/MG061 and pMG004 as template ...
-
bioRxiv - Microbiology 2022Quote: ... Libraries were prepared using a NEBNext Ultra II fragment library preparation kit (New England Biolabs, USA) with Covaris shearing (Covaris ...
-
bioRxiv - Microbiology 2022Quote: ... Purified RNA then was pulldown using m6A specific antibody using EpiMark N6-methyladenosine enrichment kit(NEB). RNA was converted into cDNA using random hexamer primers and Revert aid RT (Invitrogen) ...
-
bioRxiv - Molecular Biology 2022Quote: ... The libraries preparation was performed with Ultra II RNA Library Prep Kit for Illumina (NEB, E7770) and Multiplex Oligos for Illumina (NEB ...
-
bioRxiv - Microbiology 2022Quote: ... and library construction was executed using NEBNext Ultra RNA Library Prep kit (New England Biolabs, USA), and sequenced on a NovaSeq6000 ...
-
bioRxiv - Molecular Biology 2022Quote: ... followed by library preparation using NEBNext® Ultra™ II RNA Library Preparation Kit (NEB, E7770S). Libraries were paired-end sequenced with read lengths of 150 bp on Illumina HiSeq X Ten or Nova-seq instruments ...
-
bioRxiv - Molecular Biology 2022Quote: ... The final pool was quantified using NEBNext Library Quant Kit for Illumina (New England Biolabs, E7630L) and paired-end sequenced on an Illumina MiSeq.
-
bioRxiv - Plant Biology 2022Quote: ... Sequencing libraries were prepared with a NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) and sequenced on an Illumina HiSeqX10 platform using a 150-bp paired-end sequencing strategy at Novogene Co. ...
-
bioRxiv - Neuroscience 2022Quote: ... RNAs were used to synthesize cDNAs using the ProtoScript cDNA synthesis kit (New England Biolabs, USA) and subjected to RT-PCR analyses to determine STAU1-RNA interactions ...
-
bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were generated using NEBNext Ultra II DNA Library Prep Kit (New England BioLabs, #E7645L) according to manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... Genetic substitutions were introduced via site-directed mutagenesis using the Q5 site-directed mutagenesis kit (NEB) according to the manufacturer’s instructions ...
-
bioRxiv - Physiology 2022Quote: ... O-GlcNAc site were mutated into alanine with Q5® Site-Directed Mutagenesis Kit (NEB#E0554). Lentivirus were packed as previously described (Huang et al. ...
-
bioRxiv - Plant Biology 2023Quote: ... Sequencing libraries were generated using NEB Next Ultra RNA Library Prep Kit for Illumina (NEB, USA) as reported previously (Jiang et al. ...
-
bioRxiv - Systems Biology 2023Quote: ... we pelleted each liquid culture by centrifugation and used the Monarch Plasmid Miniprep Kit (NEB #T1010L) to isolate plasmid according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2022Quote: ... The libraries were indexed for multiplexing using NEBNext multiplex oligos kit for Illumina (New England Biolabs). A total of 54 libraries were generated ...
-
bioRxiv - Biochemistry 2022Quote: ... cDNA libraries were prepared using the NEBNext Multiplex Small RNA Library Prep kit for Illumina (NEB) in accordance with the manufacturers’ instructions.
-
bioRxiv - Neuroscience 2023Quote: ... An HpaI site within W3SL was destroyed using the Q5® Site-Directed Mutagenesis Kit (NEB): pAAV-U6-TATAlox-CMV-EGFP-TATAlox-W3SL ...
-
bioRxiv - Neuroscience 2022Quote: ... Libraries were quantified with the NEBNext Library Quant Kit for Illumina according to manufacturer instructions (NEB). Pooled libraries were combined and sequenced with a 75 cycles NextSeq 500/550 High Output Kit on a NextSeq 500/550 machine (Illumina).
-
bioRxiv - Neuroscience 2022Quote: ... Sequencing libraries were generated using the NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations ...
-
bioRxiv - Neuroscience 2022Quote: ... We quantified the libraries using the NEBNext Library Quant Kit for Illumina (New England Biolabs, #E7630) based on the mean insert size provided by the Bioanalyzer ...
-
bioRxiv - Physiology 2022Quote: ... Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (New England BioLabs) following manufacturer’s recommendations ...
-
bioRxiv - Molecular Biology 2022Quote: ... Reverse transcription was performed using a First-Strand cDNA Synthesis kit (New England Biolabs, Cat. #E6560) and random primers ...
-
bioRxiv - Molecular Biology 2022Quote: ... The synthesized cDNA was purified using Monarch® PCR and DNA Cleanup Kit (New England Biolabs). Poly-guanine was added at the cDNAs 3’ end using Terminal Transferase (Roche ...
-
bioRxiv - Molecular Biology 2022Quote: ... subsequently used to generate cDNA with LunaScript RT SuperMix Kit (New England Biolabs, catalog no. E3010S). Real-time PCR was performed using SYBR green gene expression assays (New England Biolabs ...
-
bioRxiv - Molecular Biology 2022Quote: ... and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs: E7760) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... libraries were constructed using NEBNext Ultra™ II RNA Library Prep Kit for Illumina (NEB, E7770) following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit (E7760, NEB). Quality control was checked through a Bioanalyzer ...
-
bioRxiv - Bioengineering 2022Quote: ... and library concentrations were measured with the NEBNext Library Quant Kit (New England Biolabs, Ipswich, MA). The libraries were pooled to a final concentration of 2 nM and run on a NextSeq sequencer (Illumina ...
-
bioRxiv - Molecular Biology 2022Quote: ... NEB Next Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) was used for the construction of sequencing libraries ...
-
bioRxiv - Bioengineering 2022Quote: ... RNA was reverse-transcribed and PCR amplified using Luna Universal One-Step RT-PCR Kit (NEB). SARS-CoV-2 replication was assessed by using primers specific to the N mRNA (Forward 5’-CTCTTGTAGATCTGTTCTCTAAACGAAC-3’ ...
-
bioRxiv - Cell Biology 2022Quote: ... C55S and C62S mutants were generated using the Q5 site directed mutagenesis kit (New England Biolabs). The Cx36 pcDNA plasmid was PCR amplified with modified primers to introduce the corresponding deletions or substitutions ...
-
bioRxiv - Synthetic Biology 2024Quote: ... IVT-produced RNA samples were purified using a Monarch® RNA Cleanup Kit (New England Biolabs). ssRNA during RNA extractions was degraded with RNase T1 (Thermo Scientific) ...
-
bioRxiv - Systems Biology 2023Quote: ... IVT was conducted using the HiScribe® T7 Quick High Yield RNA Synthesis Kit (NEB E2050S) - 1x T7 RNA Polymerase Mix ...
-
bioRxiv - Developmental Biology 2024Quote: ... and ligation to adapters with “NEBNext Ultra II DNA Library Prep Kit for Illumina” (NEB #E7645). Adapter-ligated libraries were completed by a PCR of 21 cycles of amplification ...
-
bioRxiv - Microbiology 2024Quote: ... and cDNA library preparation (NEBNext Ultra II Directional RNA Library Prep Kit - Bacteria (New England Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2024Quote: ... a sequencing library was prepared using NEBNext® Enzymatic Methyl-seq Kit (NEB; catalog number E7120S). Library sequencing was performed using NextSeq 2000 (Illumina ...
-
bioRxiv - Developmental Biology 2024Quote: ... RNA-seq libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit (NEB, #E7420L), followed by sequencing on an Illumina Hiseq 4000 system ...
-
bioRxiv - Plant Biology 2024Quote: ... The sgRNA was first synthesised using the EnGen sgRNA synthesis kit New England Biolabs (NEB #E3322) before mixing with the Cas9-NLS to form the RNP complex ...
-
bioRxiv - Genetics 2024Quote: ... The resulting product was inserted into linearized pKR482 using NEBuilder HiFi DNA assembly kit (NEB E5520S) and transformed into 10-beta cells ...
-
bioRxiv - Synthetic Biology 2024Quote: ... library concentrations were quantified with qPCR using the NEBNext Library Quant Kit for Illumina (NEB, E7630S), and pre- and post-selection libraries were combined as two pools ...
-
bioRxiv - Molecular Biology 2023Quote: 7.5ug of total RNA was immunoprecipitated for m6A with EpiMark® N6-Methyladenosine Enrichment Kit (NEB) following the manufacture protocol with 10% of the sample RNA taken as input prior to the pulldown ...
-
bioRxiv - Microbiology 2023Quote: ... RT-qPCR was then performed using Luna Universal One-step RT-qPCR kit (New England Biolabs) following the provided protocol on a BioRad CFX Opus 96 quantitative Real-Time PCR machine ...
-
bioRxiv - Microbiology 2023Quote: ... Prepared libraries were quantified using a NEBNext Library Quant kit for Illumina E7630L (New England BioLabs) and pooled with volumes adjusted to normalize concentrations aiming for ∼700-fold genomic coverage for population samples or ∼200-fold genomic coverage for clones ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... cDNA was synthesized using the ProtoScript II Reverse Transcriptase kit (New England BioLabs, Évry-Courcouronnes, France). qRT-PCR was performed in quadruplets on a CFX384 Touch Real-Time PCR Detection System (Biorad ...
-
bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were constructed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations ...
-
bioRxiv - Biochemistry 2024Quote: ... The SUGCT insert and pcDNA5/FRT vector were ligated by using the Quick Ligation Kit (NEB) and transformed to TOP10 chemically competent cells (Thermo Fisher Scientific) ...