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Citations for New England Biolabs :
3551 - 3600 of 9358 citations for Rat Leptin RIA kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... before cDNA was synthesized with the AMV LongAmp® Taq RT-PCR kit (New England Biolabs). A 500bp fragment covering the second and third exons common to all polymorphic MHC class I genes as amplified from each cDNA using NEB Go Taq polymerase and ovine MHC class I generic primers 416 and Cr as described in36 ...
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bioRxiv - Immunology 2021Quote: ... Sequencing libraries were generated by using NEBNext UltraII FS Library Prep Kit (New England BioLabs, USA), and QC of cDNA and final libraries was performed by MultiNA (Shimazu ...
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bioRxiv - Microbiology 2021Quote: ... Constructs with these individual mutations were generated using Q5 Site-Directed Mutagenesis Kit (New England Biolabs). A 2-step PCR protocol using the Q5 mutagenesis kit was used to join nucleotides 1-117 ...
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bioRxiv - Molecular Biology 2021Quote: ... BspEI and BclI restrictions sites were introduced using Q5® Site-Directed Mutagenesis Kit (NEB, E0554). The left homology arm was cloned using Gibson assembly strategy by linearizing the vector with BspEI (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... sgRNAs were generated using the HiScribe T7 Quick High Yield RNA Synthesis Kit (New England Biolabs). Founder mice (F0 ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR was performed using Luna® Universal One-Step RT-qPCR Kit (New England Biolabs), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... DNA isolation from reaction mixtures was performed using Monarch PCR & DNA Clean-up Kit (NEB, USA). Plasmid miniprep DNA isolation was performed using Wizard Plus SV Minipreps DNA Purification System (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... Protein and non-incorporated nucleotides were removed using the Monarch RNA Clean up kit (NEB, T2040), before quantification by nanodrop.
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bioRxiv - Genomics 2021Quote: ... Libraries were generated using NEBNext®UltraTM RNA Library Prep Kit for Illumina® (NEB, USA) following manufacturer’s ...
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bioRxiv - Genomics 2021Quote: ... and the library was generated using NEBNext UltraTM RNA Library Prep Kit for Illumina (NEB, USA), following the manufacturer’s recommendations ...
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bioRxiv - Genetics 2019Quote: ... The 19 base-sgRNA was cloned into pDD162 using a Q5 mutagenesis kit (New England Biolabs), as described by 48 ...
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bioRxiv - Genomics 2021Quote: ChIP libraries were prepared while bound to Dynabeads using NEBNext Ultra II Library preparation kit (NEB) using half reactions ...
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bioRxiv - Microbiology 2021Quote: Bacterial RNA was extracted using the Monarch total RNA miniprep kit (New England Biolabs, Ipswich, MA) following manufacture’s guidelines ...
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bioRxiv - Microbiology 2020Quote: ... Point mutations rescuing individual lysine residues were introduced using the Q5 Site-Directed Mutagenesis Kit (NEB) and confirmed by sequencing ...
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bioRxiv - Developmental Biology 2020Quote: ... Sequencing libraries were generated using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... followed by cDNA NGS library preparation (NEBNext® Ultra RNA Library Prep Kit for Illumina, NEB). The size of the resulting libraries was controlled by the use of a Bioanalyzer High Sensitivity DNA Kit (Agilent Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... Libraries were quantified using a NEBNext® Library Quant Kit for Illumina (New England BioLabs, E7630L) and sequenced on either an Illumina NextSeq 500 instrument using 42bp paired-end reads ...
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bioRxiv - Microbiology 2022Quote: ... before preparation of stranded libraries using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB). cDNA libraries were sequenced on an Illumina NovaSeq 6000 to generate ~55-130 million paired-end reads of 75 bp per sample (Supplemental Table 2) ...
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bioRxiv - Immunology 2022Quote: ... then cDNA libraries were generated using a NEBNext Ultra DNA Library Prep Kit (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: All fragments were assembled using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, #E5520S) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by Gibson assembly with the NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs), was performed as described in Schaub et al ...
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bioRxiv - Biochemistry 2022Quote: In vitro coupled transcription-translation was performed at 37 °C using the PURExpress ΔIleRS Kit (NEB) following manufacturer’s protocol supplemented with [35S]-methionine (EasyTag ...
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bioRxiv - Genomics 2022Quote: ... SNVs were inserted into the regions of interest using the Q5 Site-Directed Mutagenesis Kit (NEB) and mutation-specific primers were designed with the NEBaseChanger tool ...
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bioRxiv - Neuroscience 2022Quote: DNA was purified using the Monarch PCR and DNA cleanup kit (New England Biolabs, 13-0041) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... gDNA was extracted using the NEB Monarch gDNA extraction kit and digested overnight with DpnI (NEB). DamID adaptors were then ligated to DpnI-digested DNA ...
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bioRxiv - Cancer Biology 2022Quote: ... mRNA expression was determined using SYBR green Luna One Step RT-qPCR Kit (New England BioLabs) on a C1000 Touch (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... Quenched IVT reactions were prepared using the Monarch® RNA cleanup kit 500μg (New England Biolabs), and the concentration of the mRNA eluted in 50μl was determined using the Quant-iT™ broad range RNA assay.
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bioRxiv - Molecular Biology 2022Quote: ... Genomic DNA libraries were prepared using the NEBNext Ultra II FS DNA Library Prep Kit (NEB), with the insert size in the range of 150 to 250 bp ...
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bioRxiv - Bioengineering 2022Quote: ... pooled libraries were quantified using the NEBNext Library Quant Kit (New England Biolabs, Ipswich, MA, USA) for Illumina.
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bioRxiv - Microbiology 2022Quote: ... In vitro translation reactions were performed using PURExpress ΔRF123 kit and PURExpress ΔRibosome (New England Biolabs) according to manufacturer instructions ...
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bioRxiv - Genomics 2022Quote: ... The assembled product was purified with the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030L) and eluted in 12 μl of H2O.
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bioRxiv - Biochemistry 2022Quote: ... All VPS37A1-148 mutants were generated using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). The corresponding primers are listed in Supplementary Table 2 and all constructs were verified by sequencing ...
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bioRxiv - Bioengineering 2022Quote: ... the reaction was cleaned and concentrated with a Monarch PCR & DNA Cleanup Kit (NEB catalog # T1030L) and eluted in either 10 or 12 µL of nuclease free H2O ...
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bioRxiv - Biochemistry 2022Quote: ... Total RNA was extracted using the Monarch® Total RNA Miniprep Kit (T2010S, New England Biolabs), and cDNA was generated using the SuperScript™ IV First-Strand Synthesis System (18091050 ...
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bioRxiv - Bioengineering 2022Quote: ... cDNA synthesis was performed with random primers from the Protoscript cDNA synthesis kit (NEB Cat#E6560S). Transgene expression was then quantified via qPCR using the iTaq Universal SYBR green supermix and primers binding to the mCherry transcript ...
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bioRxiv - Genomics 2022Quote: ... The PCR product was purified using the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030L). For the last PCR ...
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bioRxiv - Genomics 2022Quote: ... The product was then purified with the Monarch PCR&DNA Cleanup kit (New England BioLabs T1030L), and eluted with 20 μl of ddH2O ...
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bioRxiv - Developmental Biology 2022Quote: CUT&RUN libraries were constructed using the NEB Ultra II DNA library prep kit (NEB #E7645) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... cleaned up A-tailed DNA went through Adaptor ligation reaction using Quick Ligation Kit (NEB, M2200L) following manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... Illumina sequencing libraries were made using the NEBNext Ultra II RNA Library Prep Kit (NEB #E7775). The quality of the libraries was assessed using an Agilent Bioanalyzer DNA High Sensitivity chip ...
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bioRxiv - Genomics 2022Quote: ... Library preparation was carried out using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) and were paired-end sequenced (38bp+38bp ...
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bioRxiv - Genetics 2022Quote: ... Illumina libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... RNA was subsequently isolated following the manufacturer’s instructions of the Monarch RNA Isolation Kit (#T2010S; NEB). RIN scores and concentration were performed using High Sensitivity RNA ScreenTape (#5067-5579/#5190-6507 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Gibson assemblies were performed using the NEB HiFi Assembly Kit (New England Biolabs, Cat. No. E2621L) with 100 ng of insert and a 2:1 molar ratio of insert:vector ...
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bioRxiv - Developmental Biology 2022Quote: ... Ac5-DanFL was made by DNA assembly using NEBuilder HiFi DNA Assembly Cloning Kit (NEB #E5520S) from pAc5.1C-FLuc-V5His6 (Addgene plasmid #21183) ...
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bioRxiv - Immunology 2022Quote: ... and 1 μg was used to generate dsRNA using a HiScribe T7 RNA synthesis kit (NEB). Reaction products were purified with the GeneJET RNA Purification Kit (ThermoFisher) ...
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bioRxiv - Plant Biology 2022Quote: mRNA libraries were constructed using NEBNext® UltraTM RNA Library Prep Kit for Illumina (NEB, USA) according to the instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Sequencing libraries were generated using the NEBNext® Ultra ™ RNA Library Prep Kit (NEB, USA). After library construction ...
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bioRxiv - Synthetic Biology 2022Quote: ... The sequencing library was prepared using NEBNext Ultra II FS DNA library prep kit (NEB E7805L) with 500 ng genomic DNA as input ...
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bioRxiv - Plant Biology 2022Quote: One μg of RNA was reverse transcribed using the LunaScript RT SuperMix Kit (New England Biolabs) according to the manual ...