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Citations for New England Biolabs :
3351 - 3400 of 9843 citations for Swine IL 4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... Libraries were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645S) and size selection was performed as described above ...
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bioRxiv - Genetics 2019Quote: ... mRNAs were isolated from 1 μg of total RNA using a PolyA selection kit (NEB) and sequencing libraries were prepared following instructions from NEBs Ultra Library Preparation Kit for Illumina ...
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bioRxiv - Molecular Biology 2019Quote: ... This was achieved with the Q5® Site-Directed Mutagenesis Kit (New England Biolabs, UK) using the primers listed in the Supplementary information (Table S2).
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bioRxiv - Molecular Biology 2019Quote: ... MNase libraries were constructed with NEBnextUltra II library preparation kit (New England Biolabs, Ipswich, MA) and sequenced on an Illumina HiSeq4000 (Illumina ...
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bioRxiv - Plant Biology 2019Quote: ... cDNA was synthesized using the DyNAmo cDNA Synthesis Kit (New England Biolabs, Ipswich,MA, USA). The qRT-PCR analysis primer pairs of the corresponding genes were designed according to sequences obtained from the Phytozyme website (Supplemental table 2) ...
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bioRxiv - Synthetic Biology 2020Quote: ... The cloning was performed with the NEBuilder Hi Fi DNA Assembly kit (New England Biolabs). The enzymes mentioned were purchased from New England Biolabs ...
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bioRxiv - Synthetic Biology 2020Quote: ... The PCR products were purified with Monarch® PCR & DNA Cleanup Kit (New England Biolabs) and subcloned using StrataClone PCR cloning Kit (Agilent Technologies ...
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bioRxiv - Genomics 2020Quote: ... Paired-end libraries were prepared using the NEBNext Ultra DNA Library Prep Kit (NEB, USA) and sequenced on an Illumina HiSeq X Ten platform ...
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bioRxiv - Genetics 2020Quote: ... Libraries were prepared using an Ultra RNA Library Prep Kit for Illumina (New England BioLabs) with 150 ng of input RNA ...
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bioRxiv - Microbiology 2019Quote: ... SRNAs were cloned for Illumina sequencing using the Next® Small RNA Prep kit (NEB) and sequenced on an Illumina HiSeq1500 platform ...
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bioRxiv - Immunology 2020Quote: ... and reverse transcribed using the ProtoScript II first strand cDNA synthesis kit (New England Biolabs) with Oligo (dT ...
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bioRxiv - Genetics 2020Quote: ... sgRNA synthesis was performed using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB), and purified using the RNA Clean & Concentrator-25 kit (Zymo Research ...
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bioRxiv - Genetics 2020Quote: ... with HiFi assembly and an NEBuilder HiFi DNA Assembly Cloning Kit (E5520S, New England BioLabs) to create pENTR-BLRR ...
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bioRxiv - Molecular Biology 2020Quote: ... according to the manufacturer’s instructions and reverse transcribed with the LunaScript RT SuperMix kit (NEB). cDNA was analyzed by quantitative PCR using the CFX96 Touch Real-Time PCR Detection System (BIO-RAD) ...
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bioRxiv - Molecular Biology 2020Quote: ... adapter ligation and PCR enrichment with NEBNext Ultra RNA Library Prep Kit (NEW ENGLAND BioLabs). RNA and cDNA concentrations were determined using Qubit RNA/DNA (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... the cDNA was converted to double-stranded DNA with a second strand synthesis kit (NEB) in a 20 ml reaction ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 μg total RNA was depleted of rRNA using the NEBNext rRNA Depletion kit (NEB). To capture nascent RNA ...
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bioRxiv - Genetics 2020Quote: ... followed by library preparation using NEBNext® Ultra™ II RNA Library Preparation Kit (NEB). Libraries with barcodes were pooled at equal concentrations and sequenced on the Illumina HiSeq 1500 platform ...
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bioRxiv - Genetics 2020Quote: ... mRNA was prepared from total RNA by magnetic mRNA purification (Magnetic mRNA Isolation Kit,NEB) according to the manufacturer’s’ instructions with the following deviation ...
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bioRxiv - Genetics 2021Quote: ... All RNA samples were reverse transcribed using the LunaScript™ RT supermix kit (#E3010L, NEB), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... GFP-hRab22(S19N) (#1068) was generated using Q5 Site-Directed Mutagenisis kit (NEB, Cat# E0554S) according to manufacturer’s instructions with forward primer #1556 (TGTAGGTAAAaacAGTATTGTGTGGCGG ...
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bioRxiv - Molecular Biology 2020Quote: Sequencing libraries were prepared using the NEBNext Ultra™ II DNA Library Prep Kit (NEB). Four PCR cycles were used for the input samples and 17 cycles for the IP samples ...
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bioRxiv - Developmental Biology 2020Quote: ... quantified using NEBNext® Library Quant Kit for Illumina® (E7630, New England 641 Biolabs), and denatured and diluted per Illumina’s MiSeq instructions ...
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bioRxiv - Pathology 2021Quote: ... DNA ends were repaired and phosphorylated with the NEBNext End Repair kit (New England Biolabs). Adaptors were created by annealing oligonucleotides (P5/P6 and P7/P8 ...
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bioRxiv - Immunology 2021Quote: ... and 60 ng were used for the One Taq RT PCR Kit (New England Biolabs) according to the instructions provided by the manufacturer for mRNA quantification and reverse transcription (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... 6 were generated by PCR amplification (Phusion High-Fidelity PCR Kit, New England Biolabs #E0553) of an oligo that includes a T7 promoter ...
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bioRxiv - Synthetic Biology 2021Quote: ... applying poly-A enrichment and using the NEBNext Ultra II RNA Library Prep kit (NEB), indexed using NEBNext Multiplex Oligos (NEB) ...
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bioRxiv - Synthetic Biology 2020Quote: ... cDNA was synthesized from RNA using the Protoscript II First Strand cDNA synthesis Kit (NEB). To ensure library coverage of 500x ...
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bioRxiv - Molecular Biology 2021Quote: ... Common amplicon libraries were quantified with qPCR using NEBNext Library Quant kit (New England Biolabs) and pooled to equal concentrations ...
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bioRxiv - Molecular Biology 2021Quote: ... Libraries were prepared using the NEBNext Ultra Directional Library Prep Kit for Illumina (NEB, E7420L) according to manufacturer’s instructions and quantified with the KAPA Library Quantification Kit for Illumina (Kapa Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... Ribosomes were added to 100 nM to the PURExpress ribosome-free kit (New England Biolabs) (29) ...
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bioRxiv - Microbiology 2020Quote: ... RT-qPCR was performed using Luna Universal One-Step RT-qPCR kit (NEB, Cat. # E3005E) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The DNA was in vitro transcribed by using the HiScribe T7 ARCA mRNA kit (NEB) and capped RNA was isolated by using an RNA Clean & Concentrator (Zymo Research ...
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bioRxiv - Biochemistry 2021Quote: ... RNA-seq libraries were constructed using NEBNext Ultra II directional RNA library prep kits (NEB).
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bioRxiv - Bioengineering 2021Quote: ... The digested vector backbone was purified using Monarch DNA Gel Extraction Kit (New England Biolabs) or NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel) ...
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bioRxiv - Genetics 2020Quote: ... purified with a Monarch® DNA Gel Extraction kit (New England Biolabs #T1020S, Ipswich, MA), and incubated with RNAsecure™ RNase Inactivation Reagent (ThermoFisher Scientific #AM7005 ...
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bioRxiv - Genomics 2019Quote: ... Inserts were cloned into the pGL4.23c plasmid using NEBuilder HiFi DNA Assembly Cloning Kit (NEB), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... and libraries were prepared using the NEB Ultra FS II DNA Library Kit (NEB E6177) and amplified with 14 cycles of PCR ...
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bioRxiv - Immunology 2020Quote: ... cDNA was synthesized using ProtoScript First Strand cDNA Synthesis Kit (New England BioLabs, Inc – E6300L) with random primers for the MT-RNR transcripts ...
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bioRxiv - Immunology 2019Quote: ... Cysteine-to-alanine mutations were then introduced using the Q5 Site-Directed Mutagenesis Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Promoter truncations and mutations were generated with the Q5 Site-Directed Mutagenesis Kit from NEB according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Two micrograms of RNA were used with NEBNext Poly(A) Magnetic mRNA isolation kit (NEB). Oligo(dT ...
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bioRxiv - Biochemistry 2020Quote: ... Variants of UbiVPa were obtained using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs) (UbiVPa C193AC197A and UbiVPa C39AC193AC197A ...
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bioRxiv - Molecular Biology 2019Quote: ... in vitro translation was performed using the PURExpress In vitro Protein Synthesis kit (NEB 6800) and the non-stop ribosome complex was purified as previously described in ref.38 ...
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bioRxiv - Genomics 2019Quote: ... and ligated to Illumina multiplex adaptors according to NEBNext DNA library prep kit (NEB E6040S). Ligated DNA was purified using Agencourt AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA libraries were constructed using the NEBNext Ultra RNA Library Prep Kit (New England Biolabs) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... libraries are amplified and purified using the Monarch PCR & DNA Cleanup Kit (New England Biolabs), libraries are eluted in 25 μl nuclease free water.
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bioRxiv - Genetics 2021Quote: ... Sequencing libraries were generated using the NEBNextUltraTM RNA Library Prep kit for Illumina (NEB, USA) following the manufacturer’s recommendation and index codes were added to attribute sequence to each sample ...
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bioRxiv - Cell Biology 2020Quote: ... using the HiScribe T7 ARCA mRNA kit with poly-A tailing (NEB, Ipswich, MA, USA). A combination of 3 separate reactions was used to achieve high concentrations (≥ 45 μg ...
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bioRxiv - Cell Biology 2021Quote: ... RNA was also isolated using the Monarch Total RNA isolation miniprep kit (New England Biolabs). RNA was also extracted using the TRI Reagent (Thermo Fisher Scientific) ...