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Citations for New England Biolabs :
3551 - 3600 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were generated with the NEBNext Ultra II Directional RNA Library Prep Kit (NEB) and sequenced on a NextSeq 2000 (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... The three fragments were then ligated together using the NEBuilder HiFi DNA Assembly Kit (NEB). The resulting mixture was transformed into BAC-optimized replicator v2.0 electrocompetent cells (Lucigen) ...
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bioRxiv - Neuroscience 2024Quote: ... The ligation re-action was carried out using the NEBuilder HiFi DNA Assembly kit (NEB). The insert from the pENTR1A construct was transferred into the ‘promoter-less’ pAd/PL-DEST5 adenoviral expression vector48 via Gateway recombination cloning (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... and libraries were generated using NEBNext Ultra Directional RNA library kit (New England Biolabs, USA). These cDNA libraries were sequenced using the Illumina NovaSeq X 25B sequencer (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... The generated ntrB amplicons were purified using a Monarch→ PCR DNA clean-up kit (NEB) and Sanger sequencing was performed by Source Bioscience using ntrB_1119_F as a primer ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA libraries were prepared with NEBNext Ultra II DNA library Prep Kit (New England Biolabs) following the kit protocol and sequenced on Illumina NovaSeq within the Wellcome Sanger pipelines.
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bioRxiv - Genomics 2024Quote: ... DNA products were then purified using the Monarch PCR and DNA Cleanup Kit (NEB; T1030S), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... DNA samples were purified with Monarch Nucleic Acid Purification Kit (New England BioLabs, Ipswich, Massachusetts) prior to shearing on a Megaruptor 2 (Diagenode ...
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bioRxiv - Genomics 2024Quote: ... Library preparation was performed using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs). The libraries were sequenced on a Illumina NextSeq 500 using 2x75 bp reads.
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bioRxiv - Genomics 2024Quote: ... Genomic DNA was extracted from the wells using Monarch Genomic DNA purification Kit (NEB # T3010L). To remove biotin from un-ligated DNA ends ...
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bioRxiv - Immunology 2024Quote: ... of mRNA converted into cDNA with a LunaScript® RT SuperMix Kit (New England Biolabs) and a LightCycler® FastStart DNA Master SYBR Green I kit (Roche ...
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bioRxiv - Immunology 2024Quote: ... and sequencing libraries were created using the NEBnext Enzymatic Methyl Seq Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and site-directed mutagenesis using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs). The genes of interest were validated by Sanger sequencing (Source Bioscience).
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bioRxiv - Synthetic Biology 2024Quote: ... The PCR amplifications are then column-purified using the Monarch PCR & DNA Cleanup Kit (NEB) and assembled using NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Bioengineering 2024Quote: ... which was performed using the HiScribe T7 Quick High Yield RNA Synthesis kit (NEB #E2050S) at 37 °C for 16 h ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The libraries were prepared using the NEBNext Ultra II Directional RNA Library Preparation Kit (NEB), according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... cDNA was synthesized using PhotoScript II First-Strand cDNA Synthesis Kit (New England BioLabs, USA). Reverse transcription-polymerase chain reaction (RT-PCR ...
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bioRxiv - Genomics 2024Quote: ... Genomic DNA was extracted using the Monarch Genomic DNA Purification Kit (New England Biolabs, T3010S) and quantified using a Qubit 1X dsDNA High-Sensitivity kit (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... Library preparation was conducted with NEBNext Ultra II RNA Library Prep Kit (NEB, Catalog #E7770), including polyA selection ...
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bioRxiv - Cancer Biology 2024Quote: ... High-quality total RNA was purified using the Monarch Total RNA Miniprep Kit (NEB #T2010S) per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... then DNA breaks were end-repaired (1 hr room temperature; Quick Blunting Kit (NEB, E1201L) plus 100 µg/mL BSA ...
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bioRxiv - Genetics 2024Quote: ... site directed mutagenesis was performed using Q5 Site-Directed Mutagenesis Kit (New England Biolabs E0554) with primers 5’ CTCTGCCTTCtGTGCTCTGGC-3’ and 5’-TGGCCATGGACCACTTCC-3’ ...
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bioRxiv - Neuroscience 2024Quote: ... Weill Cornell Medicine) was mutagenized with a Q5 Site-Directed Mutagenesis kit (New England Biolabs) to the P301S mutant using forward primer 5’-ATC AAA CAC GTC TCG GGA GGC GGC and reverse primer 5’-ATT ATC CTT TGA GCC ACA CTT GGA CTG GAC ...
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bioRxiv - Genomics 2024Quote: ... DNA was cleaned using the Monarch® PCR & DNA Cleanup Kit (New England Biolabs, USA). Electrophoresis in the first dimension was performed in 0.7% ethidium bromide (EtBr)-free agarose gel in 1xTBE buffer at low voltage (0.7 V/cm ...
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bioRxiv - Biochemistry 2024Quote: RNA was produced according to our established protocols with T7 in vitro transcription kit (NEB) with labeled cy3 ...
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bioRxiv - Biochemistry 2024Quote: ... Point mutants were introduced using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs Inc) or In-Fusion cloning (Takara) ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA libraries were generated using the NEBNext Ultra II RNA Library Prep Kit (NEB #E7775) on Bravo Liquid Handler (Agilent) ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was then isolated using the Monarch® Genomic DNA Purification Kit (NEB Biolabs). Polymerase chain reaction with primers crossing the RLI insert were used to determine stability of the construct ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was then isolated using the Monarch® Genomic DNA Purification Kit (NEB Biolabs). Polymerase chain reaction with primers crossing the RLI insert were used to determine stability of the construct ...
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bioRxiv - Biochemistry 2024Quote: ... gel-purified in triplicate from a 1% gel (NEB Monarch DNA Gel Extraction Kit #T1020L), eluted in 20 μL ...
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bioRxiv - Cancer Biology 2024Quote: High-quality total RNA was purified using the Monarch Total RNA Miniprep Kit (NEB #T2010S) as per the kit protocol from wildtype USP7 cells and sent to Novogene (UK ...
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bioRxiv - Cell Biology 2024Quote: ... Reagents and general procedures from the Gibson Assembly Cloning Kit from New England Biolabs (NEB) were used ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Adaptors were added to DNA fragments using NEBNext Multiplex Oligos for Illumina kit (NEB, # E7335S) and sequencing indices were added by PCR using NEBNext Ultra II Q5Master Mix (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... Total cellular RNA was isolated using Monarch’s Total RNA Miniprep kit (New England Biolabs, #T20105) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... libraries were prepared with the NEBNext Ultra II DNA Library Prep kit (New England Biolabs), and sequenced on an Illumina NovaSeq6000 S4 v.1.5 with 150bp paired-end reads.
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bioRxiv - Genetics 2024Quote: ... we mutated the PAM sites using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs).
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bioRxiv - Cell Biology 2024Quote: ... Sequencing libraries were made using the NEBNext Ultra II Directional RNA-seq kit (NEB #E7760L) as per the manufacturer’s instructions and using the NEBNext Poly(A ...
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bioRxiv - Cell Biology 2024Quote: ... Sequencing libraries were made using the NEBNext Ultra II Directional RNA-seq kit (NEB #E7760L) as per the manufacturer’s instructions and using the NEBNext Poly(A ...
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bioRxiv - Plant Biology 2020Quote: ... The amplified NbPRS4 PCR product was gel purified (Monarch DNA Gel Extraction Kit, New England Biolabs), digested with XbaI and XhoI (New England Biolabs) ...
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bioRxiv - Biophysics 2020Quote: ... The insert was further cloned into pcDNA3.4 using the HiFi DNA Assembly Kit (New England Biolabs) and PCR products (Phusion polymerase ...
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bioRxiv - Cancer Biology 2021Quote: ... GSE1 coding sequence was modified using the Q5 Site-directed Mutagenesis Kit (E0554S, New England Biolabs) to substitute the cytosine (C ...
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bioRxiv - Cancer Biology 2021Quote: ... Sequencing libraries were generated using NEBNext® UltraTM RNALibrary Prep Kit for Illumina® (NEB, USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA was synthesized from 700ng of amplified oligos using T7 RNA polymerase (HiScribe T7 kit, NEB) following manufactural instruction ...
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bioRxiv - Cell Biology 2020Quote: The PAQR-2p∷PAQR-1∷GFP construct was generated with a Gibson assembly cloning kit (NEB) with the following three fragments ...
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bioRxiv - Cell Biology 2020Quote: ... The sgRNAs were in vitro synthesized with HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). Transcription reactions were treated with RNase-free DNaseI (NEB ...
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bioRxiv - Cell Biology 2020Quote: ... and circularized by an intramolecular ligation reaction using the Quick Ligation™ Kit (New England Biolabs)
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bioRxiv - Immunology 2021Quote: ... the mRNA was synthesized following the HiScribeTM T7 ARCA mRNA Kit (with tailing) (New England Biolabs). Once the mRNA was synthesized ...
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bioRxiv - Immunology 2021Quote: ... Site-directed mutagenesis was performed using the Q5® Site-Directed Mutagenesis Kit (New England BioLabs). Constructs were verified by Sanger DNA Sequencing at the London Regional Genomics Center ...
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bioRxiv - Developmental Biology 2021Quote: ... RNA libraries were prepared using NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs). Alternative splicing analysis of RNA sequencing data was completed using the Vertebrate Alternative Splicing and Transcript Tools (VAST-TOOLS ...
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bioRxiv - Molecular Biology 2021Quote: ... The final pool was quantified using NEBNext Library Quant Kit for Illumina (New England Biolabs, E7630L) and paired-end sequenced on an Illumina MiSeq.