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Citations for New England Biolabs :
3601 - 3650 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: The resulting PCR fragments were gel purified using the Monarch Gel Purification Kit (New England Biolabs) and TA cloned into pGEM-T Easy (Promega ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Illumina library preparation was performed with this pool using the NEBNext Ultra kit (NEB, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products from all reactions were purified with the Monarch PCR & DNA Cleanup Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... RNA sequencing libraries were generated using NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB), and sequenced on an Illumina Novaseq PE150 platform ...
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bioRxiv - Microbiology 2021Quote: ... SMU_313 with mutated SmsR4-binding site was constructed using the Q5 Mutagenesis Kit (New England Biolabs) and mutations were confirmed through Sanger sequencing.
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bioRxiv - Developmental Biology 2020Quote: ... sgRNAs were in vitro transcribed using the HiScribe T7 Quick High Yield RNA synthesis kit (NEB) using 75 ng of template ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by mutation of the MAO binding site using the Q5 site directed mutagenesis kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The cleaved DNAs were subjected to A-tailing using NEBNext Ultra II End Prep kit (NEB) and followed by ligation of an annealed hairpin adaptor (5’-phos-CGGTGGACCGATGATC/ideoxyU/ATCGGTCCACCG*T-3’ ...
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bioRxiv - Genetics 2022Quote: ... Sequencing libraries were prepared using the NEBNext Ultra™ II PCR-free kit (New England Biolabs). In short ...
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bioRxiv - Immunology 2022Quote: ... cDNA was synthesized using a ProtoScript First Strand cDNA synthesis kit (New England Biolabs, MA, E6300S) using PolyDT primers ...
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bioRxiv - Physiology 2022Quote: ... All mutagenesis was done using the Q5® Site-Directed Mutagenesis Kit from NEB (Cat # E0554S). The sequence of oligonucleotides used for this mutagenesis reaction is available on request ...
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bioRxiv - Molecular Biology 2022Quote: ... gel purified and in vitro transcribed using the HiScribe T7 high yield RNA synthesis kit (NEB) in a 20 μl reaction for 10 h at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... These were synthesised by IDT and converted into sgRNAs using the EnGen Cas9 sgRNA kit (NEB) according to instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Sequencing library preparation was performed using the NEBNext Ultra II Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... we introduced a 6-bp mutation using the Q5 site-directed mutagenesis kit (New England Biolabs). The pUAST-attB constructs were inserted into either attP40 or attP86Fb (for Piezo constructs ...
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bioRxiv - Neuroscience 2021Quote: RNA was collected from cultured granule cells using the Monarch® Total RNA Miniprep Kit (NEB). Then cDNA was reverse transcribed using SuperScript™ IV First-Strand Synthesis System (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: Library amplification: Amplification was performed with components of the NEBNext Ultra II DNA kit (NEB E7645S) and a NEBNext Multiplex Oligos set (e.g ...
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bioRxiv - Molecular Biology 2020Quote: ... or the HiScribe™ T7 Quick High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, USA) was used to transcribe the templates ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA-seq experiments were performed using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Sequencing libraries were generated using NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
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bioRxiv - Genetics 2020Quote: ... cDNA was generated using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB (UK Ltd.), Hitchen ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were prepared for sequencing using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) with NEBNext Multiplex Oligos (New England Biolabs) ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were gel purified with Monarch® DNA Gel Extraction Kit (T1020S, New England BioLabs) according to manufacturer’s instructions and sent for NGS at the Gurdon Institute NGS core using an Illumina HiSeq 1500.
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bioRxiv - Genomics 2020Quote: ... The library was then prepared using the NEBNext Ultra II direction Library preparation kit (NEB, E7760), and sample cleanups were performed using the Omega NGS Total Pure Mag Beads (Omega ...
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were constructed using NEBNext UltraTM RNA Library Prep Kit for Illumina (#E7530L, NEB, USA) following the manufacturer’s recommendations and index codes were added to attribute sequences to each sample.
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bioRxiv - Immunology 2021Quote: ... In-vitro-transcription was carried out using the Hi-Scribe RNA transcription kit (New England BioLabs). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA samples (800ng) were depleted of rRNA using the NEBNext rRNA Depletion kit (New England Biolabs). RNA-seq libraries were prepared using the NEBNext Ultra Directional RNA Library Prep kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2021Quote: ... mRNA-seq libraries were prepared using the NEBNext ultra (or ultra II) Directional RNA kit (NEB) with the NEBNext Poly(A ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... Genomic DNA (gDNA) was extracted using the Monarch Genomic DNA Purification Kit (New England BioLabs, #T3010S) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Sequencing libraries were prepared without rRNA depletion using NEBNext Ultra II Directional RNA Library kit (NEB) with the following modifications ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 μg of linearised DNA was then used in a HiScribe T7 synthesis kit (NEB), before DNase treatment and purification using a PureLink RNA mini kit (Thermo Fisher).
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bioRxiv - Cancer Biology 2020Quote: ... ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Preparation Kit (NEB, #E7645). Libraries were analyzed for fragment size using the Bioanalyzer High-Sensitivity DNA Kit (Agilent ...
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bioRxiv - Zoology 2020Quote: ... we prepared PCR-free libraries using NEBNext Ultra II DNA library prep kit (New England Biolabs), but with TruSeq DNA Single Indexes (Set B ...
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bioRxiv - Cancer Biology 2020Quote: ... NEB Next Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA) was used for the construction of sequencing libraries ...
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bioRxiv - Developmental Biology 2020Quote: ... Dppa3 and mutant Dppa3 (R107E) mRNA were synthesized using HiScribe T7 ARCA mRNA kit (NEB, E2060S), and purified using RNeasy mini kit (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... 1µL Random Primer Mix (ProtoScript II First Strand cDNA Synthesis Kit, New England Biolabs, https://www.neb.ca) was added to 7 µL extracted RNA and denatured on SimpliAmp thermal cycler (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... library preparation through Next® Ultra™ RNA Library Prep Kit for Illumina (New England Biolabs) and HiSeq 2500 paired-end 150 bp sequencing.
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bioRxiv - Developmental Biology 2020Quote: ... The sequencing-ready cDNA libraries were quantified with the NEBNext library quantification kit for Illumina (NEB). mRNA-seq libraries were sequenced with single-end 50 at ≈20 million reads per sample with biological triplicates ...
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bioRxiv - Microbiology 2020Quote: ... Gibson assembly was performed using NEBuilder® HiFi DNA Assembly Cloning Kit (New England Biolabs Inc.) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2021Quote: gDNA was extracted with the Monarch Genomic DNA purification kit (New England Biolabs; Ipswich, MA, USA) from 1ml of culture.
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bioRxiv - Genetics 2021Quote: ... DNA from the excised fragments was extracted using the Monarch® DNA Gel Extraction Kit (NEB). Before cloning ...
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bioRxiv - Evolutionary Biology 2021Quote: ... HYL1− knockout and transgenic ancFpHYL1 seedlings using the Monarch Total RNA Miniprep kit (New England BioLabs), following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... smaller DNA fragments (<100 bp) were purified using the Monarch PCR Purification Kit (New England Biolabs) with a modified protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Circularized ssDNA was next purified and concentrated using a Monarch® PCR & DNA Cleanup Kit (NEB). 20 pmol of the purified product was then incubated with 25 pmol of a complementary primer in 1X T4 DNA ligase buffer (NEB ...
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bioRxiv - Biochemistry 2020Quote: ... to remove DNA templates and cleaned up with Monarch RNA Cleanup Kit (50μg) (New England Biolabs) and eluted in nuclease-free water ...
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bioRxiv - Immunology 2021Quote: ... The resistance marker was assembled left of the amplified fimS regions using NEBuilder assembly kit (NEB). The assembled DNA fragments were PCR amplified using primers 130 and 131 and integrated into the chromosome of the respective strains instead of the endogenous fimS using lambda red recombination (Datsenko & Wanner ...
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bioRxiv - Biochemistry 2020Quote: RNA was transcribed from PCR-amplified inserts using the Hiscribe T7 high yield synthesis kit (NEB). 0.1µg/µl of the RNA of interest was heated to 90°Cfor 2 mins and cooled at 4°Cfor 2 mins before adding chilled 10X RNA structure buffer (500mM Tris pH 7.4 ...
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bioRxiv - Biochemistry 2020Quote: ... All RNA synthesis was performed using the HiScribe T7 High Yield RNA synthesis kit (NEB E2040S) according to the manufacture’s protocol ...
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bioRxiv - Bioengineering 2020Quote: IVT was carried out using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB E2050S) with the addition of RNAsin (Promega N2111) ...
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bioRxiv - Bioengineering 2021Quote: ... The amplicons and the linearized plasmid were assembled using an NEBuilder HiFi DNA assembly kit (NEB). The assembled plasmid was named pLenti-NG-PE2-BSD.