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Citations for New England Biolabs :
3751 - 3800 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... Libraries were constructed using a NEBNext® Ultra™ RNA Library Prep Kit (#E7530L, NEB, USA) (mRNA) ...
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bioRxiv - Pathology 2022Quote: ... qRT-PCR analysis was carried out using the Luna Universal One-Step RT-qPCR kit (NEB) using 100 ng RNA as input according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2022Quote: ... RNA-seq libraries were constructed using NEBNext Ultra RNA Library Prep Kit (NEB, Ipswich, MA, USA). Sequencing libraries were multiplexed and clustered on 1 lane of a flow-cell.
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bioRxiv - Genetics 2022Quote: ... The fragments amplified were purified using Monarch® DNA Gel extraction Kit (T1020S NEB, Ipswich, Massachusetts). The cwr-1 chimeras were made by fusing two fragments ...
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bioRxiv - Immunology 2022Quote: ... dephosphorylated pGEX-4T1 backbone were ligated using the T4 DNA Ligase kit (M0202M, New England Biolabs). Ligation products were transformed into ElectroMAX DH10B competent cells (18290015 ...
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bioRxiv - Cancer Biology 2022Quote: ... and NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs, E6420S). NextSeq 550 PE 75 HO (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs, NEB, #T2010S). RNA-seq libraries were constructed using the Swift Rapid Library Prep Kit (Swift Bio #R2096 ...
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bioRxiv - Cancer Biology 2022Quote: ... Total RNA was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs, NEB, #T2010S). RNA-seq libraries were constructed using the Swift Rapid Library Prep Kit (Swift Bio #R2096 ...
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bioRxiv - Cell Biology 2022Quote: ... pIREShyg2-HA-hAdipoR2-cMYC(H202A, H348A, H352A) construct was generated using Gibson-assembly cloning kit (NEB) with the following primers ...
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bioRxiv - Biochemistry 2022Quote: ... and RNA-seq libraries were prepared using NEBNext Ultra Directional RNA Library Prep Kit (E7760, NEB).
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bioRxiv - Cancer Biology 2022Quote: ... Reverse transcription and subsequent PCR amplification was done with OneTaq RT-PCR Kit (New England Biolabs). Primers ...
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bioRxiv - Cancer Biology 2022Quote: RNA for qPCR was extracted using the Monarch Total RNA Miniprep kit (T2010, New England Biolabs), and reverse transcription performed using LunaScript RT SuperMix (E3010 ...
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bioRxiv - Bioengineering 2022Quote: Genomic DNA was extracted using NEB Monarch HMW DNA extraction kit (T3060, New England Biolabs, France) according to manufacturer instructions with the following modifications ...
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bioRxiv - Developmental Biology 2022Quote: ... Up to 1000 ng of purified PCR product was used with the HighScribe T7 kit (NEB) and incubated overnight at 37°C ...
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bioRxiv - Immunology 2020Quote: ... and sequencing libraries prepared using the NEB Next Ultra II Directional RNA kit (NEB, Ipswich, MA) then sequenced to a depth of at least 20 M single-end 75 bp reads using NextSeq 500 (Illumina ...
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bioRxiv - Plant Biology 2020Quote: ... the sequencing library was prepared using the NEBNext® Multiplex Small RNA Library Prep Kit (NEB) and the resulting library was loaded onto an Illumina HiSeq X machine for PE150 sequencing.
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bioRxiv - Immunology 2020Quote: ... PCR was performed with a Phusion High-Fidelity PCR Kit (New England Biolabs, Ipswich, MA, USA) in 20µL reactions mixed with 2µL cDNA and 10µM primers ...
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bioRxiv - Bioengineering 2020Quote: ... The PCR product was purified using Monarch® Nucleic Acid Purification Kit (New England Biolabs Inc.).
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Combinations of maternal-specific repressive epigenetic marks in the endosperm control seed dormancybioRxiv - Molecular Biology 2020Quote: mRNA was isolated from total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Kit (NEB) and cDNA libraries were constructed using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB) ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were prepared with the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB, #E7420S). Sequencing was performed by LAFUGA on an Illumina Hiseq 1500 using 50 bp paired end run.
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bioRxiv - Molecular Biology 2020Quote: Library preparation was performed using the Ultra II DNA Library prep kit for Illumina (NEB, #E7645S) according to manufacturer instructions ...
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bioRxiv - Bioengineering 2020Quote: ... and DNA assembly with NEBuilder kit were done according to the manufacturer recommendations (New England Biolabs). DNA sequences of constructs were confirmed by sequencing with the appropriate primers ...
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bioRxiv - Developmental Biology 2020Quote: ... The amplified products were cloned using the NEB PCR cloning kit (New England Biolabs, Ipswich, MA). Clones from each founder were Sanger sequenced using the NEB analysis primers to define indels.
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bioRxiv - Cell Biology 2020Quote: ... gel purified and ligated with pENTR (w158-1)-backbone by Quick Ligation kit (New England Biolabs) to create pENTR-GRET ...
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bioRxiv - Genomics 2020Quote: ... followed by DNA clean-up using the Monarch PCR & DNA Cleanup Kit (New England BioLabs T1030S). Library preparations were quantified using the using KAPA library quantification kit (Kapa Biosystems KK4824 ...
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bioRxiv - Microbiology 2020Quote: ... and PCR mixes using the Q5® High-Fidelity PCR Kit (New England Biolabs, MA, USA) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Library construction was done using NEBNext Ultra™ II DNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... and subject to library preparation using NEBNext® Ultra™ II DNA Library Preparation Kit (NEB). Libraries with barcodes were pooled and sequenced on the Illumina HiSeq 1500 platform ...
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bioRxiv - Molecular Biology 2020Quote: DNA libraries were constructed using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645) with index primers (NEB ...
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bioRxiv - Synthetic Biology 2020Quote: ... cDNA was synthesized from 500ng RNA using the Protoscript II First Strand cDNA synthesis Kit (NEB). 1ul of cDNA was amplified by PCR with primers that amplify about 200 bp surrounding the sites of interest using OneTaq PCR Mix (NEB) ...
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bioRxiv - Microbiology 2021Quote: ... Sequencing libraries were generated using NEBNext UltraTM RNA Library Prep Kit for Illumina® (NEB, USA). The clustering of the index-coded samples was performed on a cBot Cluster Generation System using TruSeq PE Cluster Kit v3-cBot-HS (Illumina) ...
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bioRxiv - Synthetic Biology 2020Quote: ... cDNA was synthesized from 500ng RNA using the Protoscript II First Strand cDNA synthesis Kit (NEB). 1ul of cDNA was amplified by PCR with primers that amplify about 200 bp surrounding the sites of interest using OneTaq PCR Mix (NEB) ...
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bioRxiv - Genetics 2021Quote: ... DNA libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB# E7645), using Ampure XP beads for cleanup ...
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bioRxiv - Molecular Biology 2021Quote: IVT was carried out using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB E2050S) with the addition of RNAsin (Promega N2111) ...
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bioRxiv - Molecular Biology 2021Quote: ... We prepared cDNA from 1μg of extracted RNA using LunaScript® RT SuperMix Kit (NEB, #E3010). No Template and No Reverse Transcriptase controls (NTC and NRT ...
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bioRxiv - Microbiology 2020Quote: ... processed the extracted DNA with the NEBNext Microbiome DNA Enrichment Kit (New England Biolabs, Ipswich, MA), and quantified the resulting DNA using Picogreen (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: β1-tubulin wild type construct was generated by the gibson assembly (HiFi Kit, New England Biolabs) of a TubB1 sequence fragment synthesised as a gBlock by Integrated DNA Technologies (IDT ...
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bioRxiv - Genetics 2020Quote: ... We prepared the library using NEB Ultra II Directional RNA library kit for Illumina (NEB E7760) used dual index primers (from NEBNext Multiplex Oligos for Illumina ...
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bioRxiv - Plant Biology 2020Quote: ... mRNAs for PARE libraries were isolated using the NEB Magnetic mRNA Isolation Kit (New England Biolabs), custom adapters for selecting 5’-P mRNAs and primers from the TruSeq Small RNA Sample Preparation Kit (Illumina ...
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bioRxiv - Plant Biology 2020Quote: ... mRNAs for RNA libraries were isolated using the NEB Magnetic mRNA Isolation Kit (New England Biolabs). RNA libraries were produced using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... thlaspeos was extracted using the NEB Monarch Genomic DNA Purification Kit (New England Biolabs, Frankfurt, Germany).
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bioRxiv - Physiology 2020Quote: ... Prior to stranded mRNA isolation library construction using NEBnext library kits (New England Biolabs, Evry, France), the quality of the RNA pool was assessed on a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Genetics 2020Quote: ... Reverse transcriptase was performed with the ProtoScript II First Strand cDNA synthesis kit (New England BioLabs) using 1µg of total RNA with random hexamer primers ...
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bioRxiv - Genomics 2021Quote: ... One-step qRT-PCR was performed using the Luna Universal One-Step RT-qPCR Kit (NEB) and 5 ng RNA per reaction in technical duplicate with a StepOnePlus Real-Time PCR system (Applied Biosystems ...
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bioRxiv - Genomics 2021Quote: ... ribosomal RNA depletion was performed using 1 μg RNA using NEBNext rRNA Depletion kit (NEB#6310). Concentration of final libraries was measured using Qubit 2.0 Fluorometer in combination with Qubit dsDNA HS Assay Kit (Invitrogen) ...
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bioRxiv - Genetics 2021Quote: ... Libraries were generated with the NEBNext Ultra RNA Library Prep Kit for Illumina (New England Biolabs). Briefly ...
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bioRxiv - Genetics 2020Quote: The pVG344 plasmid was cloned using the NEBuilder HIFI DNA Assembly Cloning Kit (New England Biolabs) to assemble four amplified fragments ...
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bioRxiv - Genetics 2020Quote: ... without modification except for library preparation performed with NEBNext® Ultra kit (New England Biolabs®). For library preparation 600 ng of each genomic DNA were fragmented by sonication and purified to yield fragments of 150-200 bp ...
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bioRxiv - Genetics 2020Quote: ... Both cloning steps were performed with the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). The AarI-AarI fragment from pLdhGao23 was cloned into the AarI site of pScarless-dhGaoR ...
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bioRxiv - Cell Biology 2021Quote: ... mRNAs were isolated from total RNAs with Magnetic mRNA Isolation Kit (oligo(dT) beads) (NEB, E7490). First strand cDNAs were synthesized using random primers and second strand cDNAs were synthesized subsequently ...