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Citations for New England Biolabs :
3351 - 3400 of 9943 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... and were quantified with the NEBNext Library Quant Kit for Illumina (New England BioLabs E7630L). Libraries were pooled to a final concentration of 4 nM and submitted to the Johns Hopkins Genomics Research Core Facility for high-throughput sequencing on the Illumina NovaSeq 6000 platform ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was synthesized using ProtoScript® II First Strand cDNA Synthesis Kit (New England BioLabs). gli1 and ptch1 mRNA expression in the cells exposed to SAG were measured by qRT-PCR using Roche the LightCycler® 480 instrument ...
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bioRxiv - Genomics 2023Quote: ... The PCR product was gel purified using the Monarch DNA Gel Extraction Kit (NEB T2010) and used for IVT reaction using the NEB HiScribe T7 ARCA mRNA Kit (E2065S).
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bioRxiv - Microbiology 2023Quote: ... followed by purification using the Monarch Gel Extraction Kit (New England Biolabs, catalog no. T1020L).
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bioRxiv - Molecular Biology 2023Quote: ... The PCR products were purified using Monarch PCR and DNA Cleanup kit (NEB, Ipswich, MA). DNA concentration was quantified using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Resulting Fluc mRNA was purified twice by Monarch RNA cleanup kit (T2040S, NEB, Ipwsich, MA) to ensure that unincorporated nucleotides were completely removed ...
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bioRxiv - Microbiology 2023Quote: ... Gibson cloning was performed according to the protocol of the Gibson Assembly kit (NEB, E5520). To construct the Sch9-3E-NAT mutant and the matching Sch9-WT-NAT control strains ...
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bioRxiv - Immunology 2023Quote: ... and saRNA was transcribed in vitro using HiScribe T7 High Yield RNA Synthesis Kit (NEB). After DNase treatment ...
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bioRxiv - Neuroscience 2023Quote: ... followed by preparation of RNA libraries using the NEBNext Ultra RNA Prep kit (NEB #E7530) (New England Biolabs (NEB) ...
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bioRxiv - Physiology 2023Quote: ... and NEBNext Ultra RNA Library Prep Kit for Illumina (cat. E7530) were purchased from NEB. AMPure XP Beads (cat ...
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bioRxiv - Cancer Biology 2024Quote: ... NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs) and Multiplex Oligos for Illumina Systems (Cell Signaling ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplicons (approximately 3.4 kb) were gel purified using Monarch DNA gel extraction kit (NEB). Library construction ...
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bioRxiv - Cell Biology 2024Quote: ... The R36K variant was generated with the Q5® Site-Directed Mutagenesis Kit (NEB, # E0554S) from the pERLIN2-3xFlag using the manufacturer’s protocol (primers sequence ...
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bioRxiv - Neuroscience 2024Quote: ... Libraries were prepared using the NEBNext® UltraTM II Library Prep Kit (New England Biolabs). Paired-end 150 sequencing was performed using the NextSeq 500 platform (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645) and analyzed with either 2100 Bioanalyzer or TapeStation systems (Agilent) ...
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bioRxiv - Molecular Biology 2024Quote: All constructs were cloned using the NEBuilder HiFi DNA Assembly kit (New England Biolabs E2621) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were prepared using the Directional RNA Library Prep Kit (E7760S, New England Biolabs) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the Monarch® HMW DNA Extraction kit for Tissue (New England Biolabs, Ipswich, MA, USA) was used to improve DNA fragment length ...
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bioRxiv - Microbiology 2023Quote: ... Nef deletion Reverse: 5’-AGATCTACAGCTGCCTTGTAAGTCATTGG-3’) using Q5® Site-Directed Mutagenesis Kit (NEB #E0554S).
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were generated using the NEBNext Ultra RNA library prep kit for Illumina (NEB), and sequencing was performed on an Illumina NovaSeq 6000 platform ...
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bioRxiv - Molecular Biology 2023Quote: ... RT-qPCR was done with 100ng of RNA with the Luna RT-qPCR kit (NEB) using primers targeting beta actin as a control (Table S2) ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was then prepared for sequencing with the NEBNext Small RNA library Prep kit (NEB). Sequencing libraries were visualized using an Agilent 2200 Tapestation and sequencing was done using the Novaseq 6000 SP300 cartridge (Illumina).
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bioRxiv - Molecular Biology 2023Quote: ... Sequencing libraries were performed with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB) according to the published protocol50 ...
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bioRxiv - Molecular Biology 2023Quote: HLTF’s cDNA on the pcDNA3.1(+) backbone54 was mutated using Q5 Site-Directed Mutagenesis kit (NEB) to generate the C760S and R890Q mutant ...
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bioRxiv - Genomics 2023Quote: ... and the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The mSA S27A mutation was created by site-directed mutagenesis using commercially-available kits (NEB). 4NBX.B_C103 and 2X89.A_CCAA_C57 sequences were directly ordered from IDT ...
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bioRxiv - Genetics 2023Quote: RNA sequencing library preparation used the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB), following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The FBXO22-C326A mutant plasmids are generated via Q5 Site-Directed Mutagenesis Kit (NEB E0552) based on the wildtype FBXO22-HaloTag plasmid.
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bioRxiv - Neuroscience 2023Quote: ... RNA was isolated using the Monarch® Total RNA Miniprep Kit (#T2010S; New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and cDNA was generated using Protoscript II First Strand cDNA Synthesis Kit (New England Biolabs). SARS-CoV-2 was detected by PCR using the 2019-nCoV RUO Kit (IDTDNA ...
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bioRxiv - Plant Biology 2023Quote: ... First-strand complementary cDNA was synthesized with LunaScript™ RT SuperMix Kits (New England Biolabs). Quantitative PCR was performed with a CFX96 Touch Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Mutations on mEGFP-ATP7B were prepared following Q5® Site-Directed Mutagenesis Kit (NEB #E0554) protocol ...
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bioRxiv - Microbiology 2023Quote: ... The 3 fragments were ligated using the Gibson Assembly® Cloning Kit (New England Biolabs). The created pEXG2::ΔfahA and pEXG2::ΔpanC were transformed into E ...
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Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4bioRxiv - Biochemistry 2023Quote: ... Single point mutant plasmids were constructed using Q5® site-directed mutagenesis kit (NEB, E0554S) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Libraries were prepared using a NEBNext Ultra II DNA library prep kit (NEB, Cat# E7645S) and NEBNext Multiplex Oligos for Illumina (NEB ...
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bioRxiv - Microbiology 2023Quote: ... DNA was extracted using the Monarch Genomic DNA Extraction Kit (New England Biolabs, Massachusetts, USA) before sequencing with MinION (Oxford Nanopore Technology ...
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bioRxiv - Cell Biology 2023Quote: ... and ∼250 bp fragments were gel extracted for cloning using a PCR Cloning Kit (NEB). The resulting plasmid was purified by Midiprep (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... RNAs were transcribed using the HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs). Reactions were set up as per manufacturer’s instructions with the addition of 4 units of Murine RNase Inhibitor (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... coli C2566 genomic DNA was purified using Monarch Genomic DNA Purification Kit (NEB Ipswich, MA), GM12878 genomic DNA was obtained from Coriell Cell Repositories (Camden ...
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bioRxiv - Immunology 2024Quote: ... Libraries were prepared using the NEBNext Ultra DNA Library Prep kit (E7645, New England Biolabs).
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bioRxiv - Biophysics 2024Quote: ... The purified dsDNA templates were transcribed using HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... with the exception of KLF2 which was performed using the Q5 High-Fidelity kit (NEB). For the 11q deletion variant (P3) ...
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bioRxiv - Biophysics 2024Quote: The NEB Q5® Site-Directed Mutagenesis Kit (part number: E0554S, New England Biolabs Inc.) was used to perform the mutation responsible for mEGFP following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... mRNA was transcribed with HiScribe T7 mRNA Kit with CleanCap Reagent AG (New England Biolabs) according to manufacturer’s protocol with 1 µg of amplified template ...
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bioRxiv - Microbiology 2022Quote: ... For the majority of isolates DNA was extracted using Monarch Genomic DNA Extraction kit (NEB), but in some instances a lower quantity and quality yield was obtained ...
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bioRxiv - Developmental Biology 2022Quote: ... Libraries were prepared using the FS DNA Library Prep Kit for Illumina sequencing (NEB, E7805). DNA was digested into 150 bp fragments and paired-end sequencing was performed using a NovaSeq 6000 Sequencing System ...
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bioRxiv - Neuroscience 2022Quote: ... synthesized into a large primer and inserted immediately downstream of the U6 promoter to generate pAAV.U6.shRNA.CMV.GFP.WPRE.hGH using the NEBuilder hifi DNA assembly cloning kit (New England Biolabs). Corresponding constructs with tdTomato as the reporter were generated by swapping in the 5’-MluI and 3’ AgeI U6-shRNA-CMV fragment from each pAAV.U6.shRNA.CMV.GFP.WPRE.hGH construct in place of the corresponding sequence in pAAV-U6-shRNA-CAG-tdTomato (Batool et al. ...
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bioRxiv - Genomics 2022Quote: ... The obtained fragments were library prepped using NEBNext Ultra II DNA Library Prep Kit (NEB) for Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were prepared using the NEBNext Low Input RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... Total RNA was isolated with the Monarch Total RNA Miniprep Kit (New England Biolabs, NEB) following supplier’s recommendations ...