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Citations for New England Biolabs :
3451 - 3500 of 3709 citations for 1 1' Diethyl 4 4' bipyridinium dibromide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... the cells were washed 3 times with PBS and permeabilized 5 min on ice with permeabilize sol (1xPBS, 1%RNAse inhibitor Ribovanadylcomplex (RVC, NEB,#S1402S), 0,5 % Triton X-100 (Sigma ...
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bioRxiv - Immunology 2022Quote: ... The library for sequencing was prepared using 1 µg of RNA and the NEBNext Ultra kit (New England BioLabs, Ipswich, MA), according to the protocol for low-input samples ...
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bioRxiv - Molecular Biology 2022Quote: ... Twelve restriction enzyme digests were prepared by combining 15 µL of each purified DNA sample with 1 µL DpnI (NEB R0176S) and 4 µL 10X CutSmart Buffer (NEB ...
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bioRxiv - Bioengineering 2022Quote: ... encompassing the targeted loci were generated using 1 μl of the zebrafish lysate with Phusion® High-Fidelity Polymerase (New England Biolabs) and primers listed in Supplemental Table S3 ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was diluted 1:25 in water and used as template for RT-qPCR using the Luna Universal qPCR master mix (NEB M3003S). Primers used are listed in Supplementary Table 3 ...
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bioRxiv - Genomics 2022Quote: The 2.2 kb DNA was ligated with 1 kb biotin- and dig-labeled DNA through SbfI- and XbaI-overhangs by T4 DNA ligase (NEB Biolabs). The ligated products were attached to the anti-dig treated surface of reaction chamber and streptavidin-coated magnetic beads ...
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bioRxiv - Genomics 2022Quote: ... The RNA was removed from beads by Trizol and then followed with the 3’ adapter ligation using T4 RNA Ligase 1 (NEB M0204L). After this ...
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bioRxiv - Genomics 2022Quote: ... the SapI sgRNA expression cassette was ligated into the KpnI linearized PX458 in a 3:1 molarity ratio using T4 DNA-ligase (New England Biolabs, M0202S) according to the manufacturer’s instructions followed by transformation and Sanger sequencing to verify successful cloning ...
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bioRxiv - Synthetic Biology 2022Quote: ... which was also double checked visually in an agarose gel where the density of target bands was compared with the DNA ladder (1 Kb Plus, NEB N0469S).
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bioRxiv - Microbiology 2022Quote: ... Purified RNA was quantified by ultraviolet spectrophotometry and 1 μg used for reverse transcription by LunaScript® RT SuperMix Kit (New England Biolabs), as described by the manufacturer ...
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bioRxiv - Microbiology 2022Quote: ... A polyadenylated fraction was purified from the total RNA (1 µg) using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). This fraction was used to construct cDNA library using a previously described method [91] ...
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bioRxiv - Synthetic Biology 2022Quote: ... PCR reactions were performed with a final primer concentration of 1 mM using Q5® High-Fidelity 2X Master Mix (NEB). Equal volumes of X and Y PCR reactions were then combined ...
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bioRxiv - Plant Biology 2022Quote: ... Double promoter constructs were constructed by reamplifying the promoter region of interest with unique overhangs and cloning into the Level −1 universal acceptor backbones using the BsaI-HFv2 restriction enzyme (#R3733L New England Biolabs, USA). These parts were then assembled into the same Promoter + 5’ UTR acceptor backbone using the BpiI restriction enzyme (#ER1012 Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... PolyA RNA was then enriched from 1 μg of DNase I-treated RNA using Oligo d(T)25 Magnetic Beads (NEB S1419S), followed by RNA-seq libraries construction using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB E7420) ...
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bioRxiv - Molecular Biology 2023Quote: The starting library strand (50 pmol) and regeneration hairpin (75 pmol) (Supp. Table 1) were combined in a 1X ligation buffer (New England Biolabs (NEB)) ...
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bioRxiv - Neuroscience 2023Quote: ... PCRs to confirm targeted and untargeted SMN2 loci were performed for each of the cell lines with primer pairs shown in table 1 using High-Fidelity 2X PCR Master Mix (NEB, M0541L) and Thermal Cycler C1000 Touch (Bio-RAD) ...
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bioRxiv - Biochemistry 2023Quote: ... and ScFv16 at room temperature for two hours in the presence of 25 mU ml-1 apyrase (NEB, cat. no: M0398S) and either C3a or EP54 for complex formation ...
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bioRxiv - Genomics 2023Quote: ... Chromatin was denatured with the restriction enzyme NlaIII at a final concentration of 1 U/μL (New England Biolabs, United Kingdom) at 37°C for 18 hours ...
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bioRxiv - Bioengineering 2023Quote: ... and a gBlock (purchased from Integrated DNA Technologies [IDT]) containing an anhydrotetracycline (aTc) inducible promoter (pLTetO-1) were inserted via HiFi Assembly (HiFi Assembly Kit (NEB#E5520S)) into a PCR-ed fragment of plasmid pBbA2c-RFP (Addgene #35326 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated in CO2-balanced pre-warmed media supplied with 1 µM O6-benzylguanine (BG)-coupled Alexa Fluor 647 (NEB, S9136S) for 15 min at 37°C in a 5% CO2 atmosphere ...
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bioRxiv - Developmental Biology 2023Quote: ... pCS2-dKif6 (1-501)-EGFP was generated from the pCS2-dKif6-EGFP plasmid by Gibson assembly (NEB HiFi DNA Assembly Kit) using a GeneArt gene fragment encoding amino acids 1-501 (Thermo Fisher).
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bioRxiv - Cancer Biology 2022Quote: ... The pLentiCRISPRv2 plasmid was digested for 1 hr at 55°C with 1U per μg of DNA BsmBI-v2 (NEB, R0580), in 5 μl Buffer 3.1 and to a final volume of 50 μl ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: Purified DNA was amplified with human-or mouse-specific primers covering the RBM20 RS-domain mutation hotspot with Nextera-compatible adapters (Sup. Table 1) using Q5®Hot Start High-Fidelity 2X Master Mix (NEB). One microliter of a 1:100 dilution was used for a second PCR attaching sample-specific index barcodes (Nextera XT Index Kit v2 Set A ...
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bioRxiv - Developmental Biology 2023Quote: ... was combined with either MEP-1 or Mi-2 PCR-amplified coding sequences in a Gibson assembly reaction using NEBuilder® HiFi DNA Assembly kit (NEB) following manufacturers protocols ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated at 37°C for 20 min followed by dephosphorylation with 1 U Shrimp Alkaline Phosphatase (rSAP, New England Biolabs, #M0371S) and incubation for another 20 min ...
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Genetic gradual reduction of OGT activity unveils the essential role of O-GlcNAc in the mouse embryobioRxiv - Developmental Biology 2024Quote: ... with or w/o 500 µM auxin and the same volume of 2x Monarch DNA/RNA Protection Reagent (NEB #T2011-1) was added before snap-freezing and storage at −80°C until RNA extraction.
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bioRxiv - Cell Biology 2024Quote: ... Every 2 PCR reactions were pooled and purified in 100ul elution buffer using Monarch PCR & DNA Cleanup Kit (7:1 binding buffer, NEB#T1030L). 5ul/reaction Dynabeads M270 Streptavidin (Thermofisher#65305 ...
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bioRxiv - Microbiology 2024Quote: ... Rp6 was ligated to mono-phosphorylated transcripts at 37°C for 30 min by adding T4 RNA ligase 1 (#M0204, NEB, USA) with the buffer ...
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bioRxiv - Microbiology 2024Quote: ... The pBAD33 plasmid harboring the stem structure of rrsH (corresponding to -131∼-102 and +1559∼+1589 relative to +1 of rrsH gene) under T7 promoter was linearized by digestion with XbaI (NEB, R0145).
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bioRxiv - Genomics 2024Quote: ... 1 µg of genomic DNA was pooled with 1:20 dilutions of unmethylated lambda (1 µl of 0.1 ng/µl) and methylated pUC19 control DNA (1 ul of 0.005 ng/µl) from the EM-seq kit (E7120L; NEB, Ipswich, MA). Volumes were made up to 50 ul with 0.1x TE buffer (Sigma-Aldrich ...
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bioRxiv - Genomics 2024Quote: ... Supplementary Table 1) using a modified protocol of the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs, UK) (Supplementary Methods and Supplementary Table 18 ...
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bioRxiv - Pathology 2024Quote: Sequencing library was generated from total mRNA (1 µg) using NEBNext UltraTM RNA Library Prep Kits for Illumina (New England BioLabs, MA). cDNA libraries were sequenced by a NovaSeq 6000 (Illumina ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were stained with ush Stellaris and lacZ Stellaris probes (Frampton et al., 2022) and nuclei were stained with DAPI (1:1000, NEB 4083). Samples were mounted in ProLong Diamond antifade mountant (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... with 1 µg of total RNA used for sequencing library construction with NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA). Raw FastQ files were mapped using Bbsplit from the Bbtools 39.01 suite against Mm10 and hg38 (31 ...
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bioRxiv - Zoology 2024Quote: ... PCR products were electrophoresed and visualized on 1% agarose gels and then were cleaned using Exonuclease I and Shrimp alkaline Phosphatase (New England Biolabs Inc.). Both forward and reverse strands were sequenced using BigDye® Terminator v3.1 cycle sequencing kit ...
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bioRxiv - Neuroscience 2024Quote: ... was added at 1:6 concentration to each sample and the Quick-Load® Purple 100 bp DNA Ladder (#N0551, NEB) was used as a size indicator ...
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bioRxiv - Molecular Biology 2024Quote: ... Beads were washed thrice with 1× NEB CutSmart Buffer followed by dephosphorylation by using Quick CIP (New England Biolabs, cat # M0525S) and DNase I treatment at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and the biotin-dNTPs were removed from unligated ends by resuspending the pellet in 100 μl of 1X NEB buffer #1 (NEB B7001S) and 5 U/μl exonuclease III (NEB M0206L ...
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bioRxiv - Molecular Biology 2024Quote: ... Oligo pools were cloned into the vector at a 6:1 molar ratio by Gibson Assembly at 50°C for 30 minutes (NEB E2611). The plasmid pools were ethanol precipitated ...
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bioRxiv - Molecular Biology 2024Quote: ... Poly(A)-tailing was performed by mixing 1 µg of DNase-treated total RNA with the following reagents: 2 µL of poly(A)-tailing buffer (NEB, #B0276SVIAL), 2 µL of 10 mM ATP (NEB ...
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bioRxiv - Neuroscience 2024Quote: ... and then 5 μL of 10% NP-40 and 10X GlycoBuffer 2 as well as 1 μL of PNGase F (New England Biolabs #P0704S) were added to make a final reaction volume of 50 μL ...
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bioRxiv - Neuroscience 2024Quote: ... 12 20-μL reactions of gibson assembly were performed for 1 hour at 50C using the HiFi DNA Assembly MasterMix (NEB, E2621), with each reaction containing 184.9 ng of SacII-digested plasmid and a 10-fold molar excess of PCR-amplified insert ...
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bioRxiv - Genomics 2024Quote: ... Cells were resuspended in 2ml ligation buffer 1 (1460 µl water, 400 µl 10x T4 DNA ligase reaction buffer (NEB, B0202SVIAL), 100 µl T4 DNA ligase (NEB ...
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bioRxiv - Genetics 2024Quote: ... and up to 1 μg was reverse transcribed to cDNA using the ProtoScript II First Strand cDNA Synthesis Kit (NEB, Cat.E6560) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The soluble fraction was collected by high-speed centrifugation at 12,000 x g for 1 hr and applied to an Amylose resin high-flow column (NEB Cat#E8022S). Unbound proteins were removed by washing with sonication buffer ...
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bioRxiv - Microbiology 2024Quote: ... and sgRNAs were amplified using P5 primers with different numbers of stagger regions pooled together (for sequencing diversity) and P7 primers (Supplementary Data 1) with unique barcode sequences using Q5 High-Fidelity DNA Polymerase (New England Biolabs, #M0491) under the following PCR condition ...
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bioRxiv - Microbiology 2024Quote: ... and sgRNAs were amplified using P5 primers with different numbers of stagger regions pooled together (for sequencing diversity) and P7 primers (Supplementary Data 1) with unique barcode sequences using Q5 High-Fidelity DNA Polymerase (New England Biolabs, #M0491) under the following PCR condition ...
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bioRxiv - Microbiology 2024Quote: ... The annealed insert (2 µl of a 1:40 dilution) was ligated into 50 ng BsaI-digested vector using T4 DNA ligase (NEB M0202). Plasmids were transformed into electrocompetent pir+ E ...
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bioRxiv - Genomics 2024Quote: ... NaCl 5M stock was added to reach 1 M final concentration along with 30 µl of Streptavidin magnetic beads (NEB # S1420S) for 2 hrs with end-over-end mixing at 4°C to capture biotinylated DNA fragments ...
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bioRxiv - Immunology 2024Quote: ... This was followed by eight washes with NET-2 buffer and one wash with 500 μl of PNKT buffer containing 1* T4 polynucleotide kinase (PNK) buffer (NEB, M0201L) and 0.1% (v/v ...