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Citations for New England Biolabs :
3301 - 3350 of 10000+ citations for Thyroxine T4 ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and the seven fragments were ligated at equal molar ratios with 10,000 units of T4 ligase (New England Biolabs) in 100 µL at 16°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... Oligos were annealed and phosphorylated using equimolar ratios of oligo pairs with 1X T4 Ligation Buffer (New England Biolabs) and T4 PNK (New England Biolabs ...
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bioRxiv - Neuroscience 2022Quote: ... gRNA target region was cloned into pCAG-mTagBFP-2A-EGXXFP vector using Esp3I (Thermo Fischer) and T4 Ligase (NEB) using oligo annealing of 5’ctagTTCCTCCTATGTAGAGAGCCGTCACGAAT3’ and 5’aattATTCGTGACGGCTCTCTACATAGGAGGAA3’ ...
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bioRxiv - Microbiology 2022Quote: ... The ligation of the digested insert into the recipient plasmid was performed using T4 DNA ligase (New England Biolabs) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were incubated at 37°C for 1 h after the addition of T4 PNK (New England Biolabs M0201) to allow 5’-hydroxyl repair ...
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bioRxiv - Systems Biology 2023Quote: ... we combined 50 ng of backbone with 25 ng of annealed oligo along with T4 ligase buffer (NEB #B0202S), T4 ligase (#M0202S) ...
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bioRxiv - Neuroscience 2022Quote: ... The annealed DNA oligos of the sgRNA was ligated to the linearised lentiCRISPRv2 vector by T4 DNA ligase (NEB).
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bioRxiv - Molecular Biology 2022Quote: ... doner RNA and the complementary splint DNA strand were annealed at a molar ratio of 1: 2: 1.5 in T4 DNA ligase buffer (NEB) by incubation for 3 min at 65°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... The inserts were generated using PCR or gBlock synthesis (IDT) and were ligated either by T4 ligase (NEB #M0202M) or In-Fusion (Takara #102518 ...
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bioRxiv - Systems Biology 2024Quote: ... sgRNAs for follow-up KOs were ordered as ssDNA oligos from Azenta and cloned in BsmBI digested pXPR_BRD023 with T4 DNA ligase (NEB). pTRIP-UbC-Blast-2A-STING-mNeonGreen was expressed in 293T STING-mNG and was described in Gentili et al 20238 ...
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bioRxiv - Microbiology 2024Quote: ... the denatured dsRNA was mixed with the DNA adaptor for adaptor ligation using T4 RNA ligase 1 (M0204, NEB). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg non-methylated NEAT1_1 IVT product was radio-labelled with radioactive labelling mix (1 µL 10x PNK buffer, NEB, 1 µL NEAT1_1 IVT product, 1 µL T4 PNK, NEB, 1 µL γ-32P-ATP ...
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bioRxiv - Microbiology 2024Quote: ... 1 µl of the annealed oligos were phosphorylated in a 10 µl reaction containing 0.25 µl T4 PNK (10000 units/ml, NEB) and 1 µl 10x T4 ligase buffer for 40 min at 37°Ϲ before heat-inactivation at 65°Ϲ for 20 min ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 μM fluorescently tagged 3’ adapter (MultiplexDX) were ligated with T4 Rnl2(1–249)K227Q (M0351, New England Biolabs) overnight at 4°C and washed three times with PNK/ligation buffer ...
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bioRxiv - Genomics 2024Quote: ... The ligation of vectors with gRNA inserts was performed overnight at 4°C using T4 DNA ligase (NEB, M0202). The ligation products were then transformed ...
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bioRxiv - Immunology 2024Quote: ... top and bottom strand oligos (1 µl of 100 µM each) were phosphorylated with T4 PNK (New England Biolabs) for 30 minutes at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... 0,5 μM fluorescently tagged 3’ adapter (MultiplexDX) were ligated with T4 Rnl2(1–249)K227Q (M0351, New England Biolabs) overnight at 4°C and washed three times with PNK/ligation buffer ...
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bioRxiv - Biochemistry 2024Quote: ... was 5’ end labelled in a final volume of 10 μl using 0.6 μl T4 PNK (10U/μl, M0201 New England Biolabs) with 2 μl gamma 32P ATP 3000Ci/mmol (SRP-301 Hartmann) ...
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bioRxiv - Cancer Biology 2024Quote: ... Forward and reverse primers (100 µM) were phosphorylated and annealed using T4 Polynucleotide Kinase (PNK; New England Biolabs M0201S) and 10x T4 Ligation Buffer (Thermo B69) ...
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bioRxiv - Molecular Biology 2024Quote: ... The crosslink bearing oligo and an uncrosslinked control was then phosphorylated by T4 polynucleotide kinase (New England Biolabs M0201S).
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bioRxiv - Developmental Biology 2023Quote: ... The RNA was then end-repaired and poly(A) tailed on-bead by treatment first with T4 PNK (NEB) and then treatment with yeast poly(A ...
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bioRxiv - Microbiology 2023Quote: 10 μM solutions of each protospacer oligonucleotide were end-phosphorylated with T4 polynucleotide kinase (NEB cat. # M0201, Ipswich, MA) for 30 minutes in the presence of 1x T4 ligase buffer then annealed by heating to 95°C in a heat block which was subsequently allowed to cool to room temperature ...
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bioRxiv - Biophysics 2023Quote: RNAs were labeled with γ32P-ATP using T4 polynucleotide kinase (PNK) according to the manufacturer conditions (New England Biolabs). Radioactive RNAs were separated from any non-incorporated γ32P-ATP via denaturing 15% polyacrylamide (19:1 ...
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bioRxiv - Microbiology 2023Quote: ... The barcode fragment and pKS1 were both digested with NcoI and BamHI and ligated with T4 DNA ligase (NEB) at a 5:1 (insert:vector ...
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bioRxiv - Synthetic Biology 2022Quote: ... These were phosphorylated (2 μL 100 μM oligo stock, 2 μL 10X T4 DNA ligase buffer (New England Biolabs), 1 μL T4 PNK (New England Biolabs) ...
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bioRxiv - Genetics 2023Quote: ... Library inserts were cloned into pmrPTRE-floxed-AAV with T4 ligase (Enzymatics) and transformed into chemically competent DH5α (NEB). Outgrowths were plated on LB agar plates with 100 µg/mL carbenicillin ...
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bioRxiv - Molecular Biology 2023Quote: ... and 25-34 nt fragments isolated from the gel were dephosphorylated using T4 Polynucleotide kinase (New England Biolabs, M0201L). For each library sample ...
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bioRxiv - Molecular Biology 2022Quote: ... A-tailed fragments were ligated to 50 nL of 5 mM T7 promoter containing adapters(21) with cell barcodes and UMI with 150 nL ligation mix (25 nL T4 DNA ligase (400.000U, NEB), 3.5 nL MgCl2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Oligonucleotide probe for telomere or Alu repeats was labeled with [32P]-ATP (3,000 Ci/mmol) and T4 nucleotide kinase (New England Biolabs). The membrane was hybridized in Church hybridization buffer containing a 32P-labeled probe at 42°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... These two linker sequences were connected to both termini of replicon DNA by ligation using 1,000 units of T4 DNA ligase [New England Biolabs (NEB), Ipswich ...
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bioRxiv - Microbiology 2023Quote: ... The pdxAoperon amplicon and pACYC184 was ligated after digestion using T4 DNA ligase according to the manufacturer’s protocol (New England Biolabs). The Gibson or ligated product was transformed into E ...
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bioRxiv - Microbiology 2023Quote: ... RNA and 3′ adapters were incubated at 22°C for 2.5 hr with 51 U of T4 RNA Ligase I (NEB) and 12 U of recombinant RNase inhibitor (Takara Bio ...
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bioRxiv - Biochemistry 2023Quote: The library was ligated to the barcode oligonucleotide at a 7:1 oligo:library ratio overnight at 16 °C with T4 DNA ligase (New England Biolabs). A no-insert negative control was also ligated overnight using identical conditions ...
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bioRxiv - Bioengineering 2023Quote: ... we combined 50 ng of backbone with ∼25 ng of annealed oligo along with T4 ligase buffer (NEB #B0202S), T4 ligase (#M0202S) ...
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bioRxiv - Microbiology 2023Quote: ... Fragmented RNAs were then dephosphorylated at their 3’ end using T4 Polynucleotide kinase (PNK, New England Biolabs, Cat: M0201) in MES buffer (100 mM MES-NaOH ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.0-3.0 μg of DNA was used for a ligation reaction in a volume of 1 mL using 1.0 μL of 2000 U/μL T4 DNA Ligase (NEB) for 3C experiments ...
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bioRxiv - Molecular Biology 2023Quote: ... pairs of complementary DNA oligos (table S1) were annealed and phosphorylated with T4 polynucleotide kinase [New England Biolabs (NEB)] as previously described (3) ...
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bioRxiv - Immunology 2023Quote: ... Gel extracts of vector and trib digests were ligated into pCS2+ via overnight incubation at room temperature with T4 DNA ligase according to manufacturer’s instructions (NEB). Constructs were confirmed using sequencing performed by the University of Sheffield’s Genomics core facility ...
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bioRxiv - Cell Biology 2023Quote: ... digested plasmids and amplicons were ligated using the T4 DNA Ligase according to the manufacturer’s instructions (New England Biolabs). For Gibson Assembly ...
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bioRxiv - Immunology 2022Quote: ... Then 1 μg of vaccine mRNA was ligated to 20 pmols RA3_7N adaptor: 5rApp/CTGACNNNNNNNTGGAATTCTCGGGTGCCAAGG/3ddC with 10U of T4 KQ227 RNA ligase 1 (#M0204S NEB) in the presence of 20U RNase OUT (#10777019 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 μl of iTP_3’_linker_ApoI (10 μM) and 0.5 μl of ligase (T4 RNA ligase 2, truncated - 200 000 U/ml - New England Biolabs) per reaction ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 µL of T4 DNA Ligase and 1 µL of 10 mM ATP (all reagents from New England Biolabs, Ipswich ...
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bioRxiv - Microbiology 2023Quote: ... and the seven fragments were ligated at equal molar ratios with 10,000 units of T4 ligase (New England Biolabs) in 100 μL at 16°C overnight ...
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bioRxiv - Genetics 2023Quote: ... digested pJFRC12-10XUAS-IVS-myr::GFP backbone using the following reactions conditions: 4 µL T4 ligase buffer (10x) (NEB), 20 µl plasmid backbone DNA (0.005 pmol) ...
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bioRxiv - Developmental Biology 2023Quote: ... and ligation of DNA ends between the cross-linked DNA fragments was performed in T4 ligation buffer (NEB, B0202), ATP (NEB ...
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bioRxiv - Biochemistry 2023Quote: ... in a total reaction volume of 100 µL consisting of 50 units of T4 RNA ligase 1 (NEB #M0204S), 1mM ATP ...
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bioRxiv - Bioengineering 2023Quote: After probe ligation cells were counted and added to the ligase buffer (1X T4 DNA Ligase Reaction Buffer (NEB), 0.4 mM ATP ...
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bioRxiv - Molecular Biology 2023Quote: ... Barcoded 3’ adapters (see Extended Data Table 1) were ligated using K227Q truncated T4 RNA ligase 2 (NEB, M0351L) at a concentration of 0.5 µM adapter and in the presence of 25% PEG8000 containing a homemade 10 x ligation buffer (0.5 M Tris pH 7.8 ...
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bioRxiv - Immunology 2023Quote: ... The libraries were generated by blunt-end cloning of 1,200 ng fragmented DNA into 1,000 ng PmeI linearized and dephosphorylated pHORF3 library vector (a gift from Michael Hust, Technische Universität Braunschweig) (16 hr at 16 °C, T4 DNA Ligase, NEB). The ligation reaction was purified and transformed into TG1 bacteria (Lucigen ...
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bioRxiv - Synthetic Biology 2023Quote: ... The ligation reaction was performed with 25ng of digested vector and 3.8ng of inserts using T4 DNA ligase (NEB) at 16°C for 15 hours ...