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Citations for New England Biolabs :
3151 - 3200 of 10000+ citations for Thyroxine T4 ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... 1 mM ATP and 3 units of T4 DNA ligase for 2 h at RT (all from NEB). Next ...
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bioRxiv - Cancer Biology 2019Quote: ... ligation was carried out at RT for 2h using 2000 U of T4 DNA ligase (New England Biolabs). DH5α cells (Thermo Fisher ...
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bioRxiv - Plant Biology 2019Quote: ... Purified DNA fragments were ligated overnight at 4°C with T4 DNA ligase (New England Biolabs, Ipswich MA) per manufacturer’s recommendations ...
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Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery systembioRxiv - Genetics 2019Quote: ... 9 µL of water and 0.5 µL of BbsI enzyme and 0.5 µL of T4 ligase enzyme (200 U, NEB). The mix was incubated for 10 cycles of 5 min at 37 °C and 5 min at 23 °C ...
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bioRxiv - Genomics 2021Quote: ... 1uL of the oligo mix was added to a master mix containing 1x T4 ligase buffer (NEB M0202M), 0.5 uL T4 PNK (NEB M0201L ...
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bioRxiv - Genetics 2020Quote: ... Pairs of annealed oligos and the digested vector were ligated overnight at 16°C using T4 ligase (NEB). Following transformation ...
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bioRxiv - Genetics 2020Quote: ... Oligo pairs were separately annealed and all were ligated into digested pCFD3-NS using T4 ligase (NEB, M0202). See Supplemental File 3 for detailed cloning protocols.
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bioRxiv - Immunology 2020Quote: ... followed by synthesis of the covalently closed circular DNA (cccDNA) with T7 DNA polymerase and T4 ligase (NEB). Finally ...
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bioRxiv - Biochemistry 2021Quote: ... Oligos (IDT) were cloned into lentiCRISPR-v1 (controls) or lentiCRISPR-v2 (SLC25A40 and TXNRD2) using T4 ligase (NEB). Ligation products were cloned into Escherichia coli (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... Molecular biology reagents (Phusion polymerase, restriction enzymes and T4 DNA ligase) were obtained from New England Biolabs (NEB). Sanger sequencing was performed by Genewiz Incorporated.
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bioRxiv - Biochemistry 2021Quote: Each cyclization reaction contained the following components: 1 µL 10X T4 DNA ligase reaction buffer (New England BioLabs), 2 µL water ...
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bioRxiv - Synthetic Biology 2021Quote: ... annealed by temperature decrease from 95 °C to 4 °C and phosphorylated using T4 Polynucleotide Kinase (NEB, M0201) according to manufactures’ protocol ...
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bioRxiv - Genomics 2020Quote: ... The sample was then combined with 3’ RNA Ligase Master Mix (8μL 50% PEG 8000, 2μL 10x T4 RNA Ligase Buffer (B0216L, NEB), 1.5μL nuclease free water ...
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bioRxiv - Microbiology 2020Quote: The RNA was ligated to 10.7 pmol barcode DNA linker using 200 U T4 RNA ligase 2 (NEB) overnight at 16 °C ...
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bioRxiv - Microbiology 2020Quote: ... and ligated with BsmBI-compatible annealed and phosphorylated oligos encoding sgRNAs using high concentration T4 DNA ligase (NEB). HIV-1 Gag-Pol and VSV-G plasmid sequences are available upon request.
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bioRxiv - Molecular Biology 2021Quote: ... The digested fragments were cloned into the pcDNA3 vector using T4 DNA ligase (New England Biolabs, MA, USA), according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of 100 μM SARS_139 and SARS_140 primers were mixed with 1 μl T4 Polynucleotide kinase (PNK; NEB) in 1x PNK reaction buffer (NEB) ...
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bioRxiv - Biophysics 2022Quote: ... 1 μL of 100 mM 18O4-γ-ATP followed by addition of 20 U of T4-PNK (NEB) and incubation for 1 h at 37 °C ...
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bioRxiv - Genomics 2022Quote: ... 13.1 μL Murine RNAse inhibitor, 5.25 μL Tartrazine (10 mg/mL, Carl Roth) and 13.2 μL T4 DNA ligase (New England Biolabs). 4 μL of the master mix were added to the Ligation Barcoding Plate #1 or #2 (see above ...
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bioRxiv - Genomics 2022Quote: ... DNA ends were repaired by adding 6.5ul of T4 DNA polymerase 3U/μl (New England Biolabs cat. #M0203L), 6.5ul of T4 polynucleotide kinase 10U/μl (New England Biolabs cat ...
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bioRxiv - Genetics 2022Quote: ... Insert and pLentiRNAGuide_001 were linearized with the BsmBI restriction enzyme and ligated using T4 DNA ligase (NEB #M0202S). Primers and templates are provided in Supplementary table 1 ...
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bioRxiv - Plant Biology 2022Quote: ... Ligation of universal adapters compatible with Nextera barcodes i7 and i5 was performed with T4 DNA Ligase (NEB), and Q5 Polymerase (NEB ...
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bioRxiv - Microbiology 2024Quote: ... RNA was then purified again and the eluate was subjected to circularization with T4 RNA Ligase 1 (NEB). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... Each oligonucleotide pair was phosphorylated and annealed using the T4 Polynucleotide Kinase (New England Biolabs, Ipswich, MA, USA). Duplexed oligonucleotides were ligated into BbsI (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... Ligation of the PCR product with the purified destination vector was performed using T4 DNA Ligase (M0202S - NEB) and transformed in Stbl3 bacteria ...
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bioRxiv - Microbiology 2023Quote: ... The digested DNA fragment and vector were ligated using T4 DNA ligase (New England Biolabs, Ipswich, MA, USA) at room temperature for 10 minutes and electroporated into 10β E ...
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were then inverted onto a 50μL drop on parafilm of Ligation Reaction (0.1μM DI-PLA Linker, 1X T4 Ligation Buffer (NEB, B0202), 1mM ATP ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA oligonucleotides complementary to specific RNAs (see supplementary methods) were radiolabeled with [32P]ATP using T4 PNK (NEB), and purified using G-50 columns (Fisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... The two oligos were combined in equimolar amounts and phosphorylated using T4 PNK enzyme (New England Biolabs #M0201S) for 30 minutes at 37°C and then slowly annealed in a thermocycler to make the final double-stranded fragment with sticky ends for cloning into the all-in-one AAV constructs ...
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bioRxiv - Cell Biology 2023Quote: ... to allow ligation of a pre-adenylated DNA linker with T4 Rnl2(tr) K227Q (New England Biolabs, M0351S). The DNA linker used incorporates sample barcodes to enable library multiplexing ...
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bioRxiv - Genomics 2023Quote: ... with the ligation of 3′-small RNA Tru-Seq adapter using the truncated T4 RNA Ligase 2 (NEB). 45-150 nt capped small RNAs were recovered on 15% Urea-TBE gel (Novex ...
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bioRxiv - Biochemistry 2023Quote: ... Oligos containing the spacer sequence and Esp3I sticky ends were annealed and phosphorylated using T4 Polynucleotide Kinase (NEB) and purified using the Oligo Clean and Concentrator kit (Zymo Research) ...
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bioRxiv - Bioengineering 2023Quote: ... the upstream and downstream digested dsDNA were ligated to each other by T4 DNA ligase (New England Biolabs). To unbind the ligase from DNA ...
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bioRxiv - Microbiology 2022Quote: ... Inserts were ligated into the linearized plasmid overnight at 16°C using T4 DNA ligase (New England Biolabs) following the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2023Quote: ... The linearized vector and the sgRNAs heteroduplexes were ligated overnight at 16°C using T4 DNA ligase (NEB), and the ligation product was used to transform One Shot™ Stbl3™ cells (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... Ligation of the RNA 3’ Adapter (RA3) was achieved by using T4 RNA Ligase 1 (NEB, Ipswich, MA) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... and treated with alkaline phosphatase and then annealed protospacer sequences were cloned in using T4 DNA ligase (NEB). gRNAs were cloned into the pU6-Cj-sgRNA expression plasmid (Addgene #89753 ...
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bioRxiv - Genomics 2023Quote: Cells were resuspended in 400 μl of restriction enzyme (RE) digestion mix (1x T4 ligase buffer (NEB, B0202S), 500U MseI (NEB ...
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bioRxiv - Molecular Biology 2023Quote: ... The end-polished DNA was then diluted for blunt-end intramolecular ligation by T4 DNA ligase (NEB, M0202S). Ligation reaction was carried out at 16 °C for 16-20 hr ...
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bioRxiv - Biochemistry 2023Quote: ... 13 pmol of purified RNA was phosphorylated with y-32P ATP (Hartmann Analytics) by T4 polynucleotide kinase (NEB) for 30 min at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... were generated by ligation of two annealed oligonucleotides (gRNA_top and gRNA_bottom) into the BsaI-digested pBF-gC backbone (61) using T4 DNA ligase (New England Biolabs).
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bioRxiv - Microbiology 2023Quote: Promoter DNA fragments were excised from plasmid pSR and end-labelled with γ32-ATP using T4 PNK (NEB). EMSAs and DNase I footprints were done as previously described 40 ...
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bioRxiv - Microbiology 2023Quote: ... The 3′ ends of purified RNA were dephosphorylated with the addition of T4 PNK enzyme (New England Biolabs) and 10 mM ATP was added to phosphorylate the 5’ ends of the RPF (ribosome protected fragments) ...
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bioRxiv - Genomics 2023Quote: ... The vectors and insert guides were annealed for 16 hrs overnight using T4 ligase (New England Biolabs, M0202L) at a 1:2 molar ratio of vector to insert ...
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bioRxiv - Cell Biology 2023Quote: ... 50pmol of each oligo were mixed in a 25µL reaction and phosphorylated with T4 polynucleotide kinase (M0201S, NEB). Reactions were performed for 30 min at 37°C in 1X T4 DNA ligase buffer (B0202S ...
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bioRxiv - Genomics 2023Quote: ... L1 - Adaptor.L8 with Adaptor.S (21)) was annealed with the DNA fragments by T4 DNA ligase (New England Biolabs) incubating overnight at 16 °C ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... P5 adapters were ligated to the ends with 1 μl of 2000 U/μl T4 DNA Ligase (NEB) at 16 °C for 20 hr ...
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bioRxiv - Plant Biology 2023Quote: ... Adapter Mix II (ONT, Oxford, UK), ONT Ligation Buffer (ONT, Oxford, UK) and Quick T4 DNA Ligase (NEB) were added and incubated for 10 minutes at room temperature and purified with 0.4X AMPure XP beads (Beckman Coulter ...
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bioRxiv - Biophysics 2023Quote: ... An overnight ligation was performed by mixing digested vector and annealed gRNA inserts using T4 DNA ligase (NEB) at 4⁰C ...
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bioRxiv - Cancer Biology 2023Quote: ... We performed ligation of our insert and expression vector utilizing the T4 DNA ligase (New England Biolabs, M0202S) according to manufactures protocol ...