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Citations for New England Biolabs :
3451 - 3500 of 10000+ citations for Thyroxine T4 ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: Oligonucleotide was annealed before use: 20 μl 100 pM/μl oligonucleotide and 20 μl 10x T4 DNA ligase buffer (NEB) in 200 μl final volume were incubated in a heating block 95°C 5 min then block was removed from heat and left to cool to room temperature over ~2 h.
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bioRxiv - Molecular Biology 2020Quote: ... containing 40 μl 50% PEG 8000 for 1 hour at room temperature then incubated overnight at 25°C in 100 μl 1x T4 RNA ligase buffer (NEB) containing 40 μl 50% PEG 8000 ...
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bioRxiv - Molecular Biology 2020Quote: ... then overnight at 25°C with another 100 μl of the same buffer containing 2.7 μl END-seq adaptor 1 and 1 μl high concentration T4 DNA Ligase (NEB M0202M). After rinsing twice with 1 ml tris buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... the samples were treated with Klenow exo- at 37 °C for 30 min to fill the ends of adaptors and then trimmed with T4 DNA polymerase (NEB) at 12 °C for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Ribodepleted RNA were then ligated to 10 pmol of a biotinylated 3’ adapter, (3’-Adap TAIL-seq, Supplementary Table 7) using 10 units of T4 RNA ligase 1 (NEB) in a final volume of 10 μl for one h at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... in a final volume of 50 μl for 1 h at 37°C and 1X T4 of RNA Ligase Reaction Buffer (NEB, 50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... phosphorylated and adenylated in 50 μl reaction volume using the following final concentrations: 1X T4 DNA ligase buffer (NEB #B0202S), 0.5 mM each dNTP (Roche #KK1017) ...
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bioRxiv - Biophysics 2022Quote: ClpA pore-2 mutations were introduced using round-the-horn mutagenesis with T4 polynucleotide kinase and Q5 high-fidelity polymerase (New England Biolabs) into pET9a-M169TClpA ...
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bioRxiv - Cell Biology 2022Quote: ... In-house adapters containing 8-nucleotide unique molecular identifier (UMI) sequences were ligated onto the DNA fragments with T4 DNA ligase (NEB). Excess adapters were removed by AMPure XP beads (Agencourt ...
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bioRxiv - Genomics 2019Quote: ... Bead-nuclei were pelleted for 2 min at 2500 g and resuspended in 189 µL blunting solution (1× T4 DNA ligase buffer (NEB), 0.25 mM dNTPs ...
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bioRxiv - Genomics 2019Quote: ... 400 ng of purified DNA was ligated overnight at 16 °C to adapters (Table S4) with T4 DNA ligase (NEB). Ligated DNA was purified using MiniElute columns (Qiagen) ...
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bioRxiv - Genomics 2020Quote: ... Irradiated and control DNA was either loaded directly on a 1% agarose gel or incubated with T4 endonuclease V (New England Biolabs) for 30 minutes at 37°C and subsequently analyzed by agarose gel electrophoresis using a 1% agarose gel.
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bioRxiv - Molecular Biology 2021Quote: A 50-100 ng of IP-DNA or Input-DNA were end repaired by a mix of T4 DNA polymerase (NEB), T4 PNK (NEB) ...
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bioRxiv - Genetics 2019Quote: ... 2011) (Table S2-S3) were ligated to the digested DNA with 1 U of T4 DNA ligase (New England Biolabs) in 50 μl of reaction volume at 16°C for 5 h and inactivated at 65°C for 10 minutes ...
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bioRxiv - Synthetic Biology 2019Quote: Primers for the amplification (Eurofins Genomics, Ebersberg, Germany) were phosphorylated by the T4-polynucleotidekinase/-buffer (New England Biolabs, Ipswich, USA) prior to amplification via PCR (Q5® High-Fidelity Polymerase ...
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bioRxiv - Genomics 2019Quote: ... and then ligated into PiggyBac plasmid pB-TRE3G-BsmBI-EF1α-HygroR-P2A-rtTA by Golden Gate Assembly with enzyme BsmBI and T4 ligase (NEB).
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bioRxiv - Genomics 2019Quote: ... and then ligated into PiggyBac plasmid pB-TRE3G-BsmBI-EF1α-PuroR-P2A-rtTA by Golden Gate Assembly with enzyme BsmBI and T4 ligase (NEB). The ScFV-sfGFP-GB1-NLSSV40 fragment was amplified by PCR from plasmid pHR-scFv-GCN4-sfGFP-GB1-NLS-dWPRE (Addgene Plasmid # 60906 ...
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bioRxiv - Genomics 2019Quote: ... The targeting sgRNA expression plasmids were made by replacing the lethal gene ccdB with annealed oligo using Golden Gate cloning with enzyme BsmBI and T4 ligase (NEB). For the sequence of each sgRNA construct.
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bioRxiv - Microbiology 2019Quote: ... The plasmid was circularized again by incubating the phosphorylated product with 400 U of T4 DNA ligase (New England Biolabs) for 2 hours at 16°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 pmoles of ssDNA were phosphorylated with 0.7 µl of [γ-32P]-ATP (7 µCi, Hartman Analytic) using T4 polynucleotide kinase (10 U, NEB). The reaction was supplemented with 10 mM DTT and incubated for 30 min at 37°C ...
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bioRxiv - Developmental Biology 2019Quote: ... gRNA vectors were generated by annealing of antisense oligonucleotide pairs and subsequent ligation into pBbsI-linearized pJJR50 by T4 ligase (New England Biolabs). All DNA vectors used for genome editing were transformed into DH5α competent cells and subsequently purified by midiprep (Qiagen).
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bioRxiv - Systems Biology 2020Quote: ... One microgram of purified RNA was fragmented at 95 °C for 7 min in 1X T4 RNA Ligase buffer (NEB) with an equal volume of 2X alkaline fragmentation buffer (0.6 volumes of 100 mM Na2CO3 plus 4.4 volumes of 100 mM NaHCO3) ...
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bioRxiv - Biochemistry 2019Quote: ... MCherry-TRAX-C1 plasmid was constructed by inserting TRAX gene between BamHI and EcoRI restriction sites in mCherry-C1 backbone using T4 DNA ligase (NEB). Plasmid transfections and siRNA knock-downs were done using Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Molecular Biology 2019Quote: ... universal adaptors were ligated to the A-tailed DNA fragments at room temperature for 1 h with T4 DNA ligase (New England Biolabs) and amplified with Illumina barcoded primers using KAPA SYBR FAST qPCR Master Mix for 5∼12 PCR cycles to obtain enough DNA for sequencing ...
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bioRxiv - Synthetic Biology 2020Quote: ... All digested products were column purified and mixed at a 6:1 molar ratio in the presence of T4 DNA ligase (NEB).
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bioRxiv - Cancer Biology 2019Quote: ... Cas9 was PCR amplified and inserted into the multiple cloning site by digestion with EcoRI and BamHI followed by overnight ligation with T4 ligase (NEB).
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bioRxiv - Genetics 2021Quote: ... Ligation was performed in a total volume of 20 μl by the addition of 1 μl T4 DNA Ligase (400 Units, NEB) in 1X T4 DNA Ligase buffer (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... A nick at the anticodon end of the DNA-RNA hybrid was filled using T4 DNA ligase and SplintR ligase (NEB). MyOne-C1 Streptavidin Dynabeads (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... which were then digested by SacI/MscI and ligated with SacI/MscI-digested second PCR fragment by T4 DNA ligase (M0202S; NEB). Linear cc2 constructs were obtained by SacI/KpnI digestion of the resulting plasmids and transformed into desired strain for cc2 insertion.
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bioRxiv - Biochemistry 2020Quote: ... 20 pmol of the purified product was then incubated with 25 pmol of a complementary primer in 1X T4 DNA ligase buffer (NEB) supplemented with 40 μM dNTPs ...
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bioRxiv - Microbiology 2021Quote: The resulting fragment was ligated into VSV NG-P (Jia et al., 2020) plasmid linearized with MluI and NotI using T4 ligase (NEB).
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bioRxiv - Plant Biology 2020Quote: ... after which barcoded adapters amenable to Illumina sequencing were added by ligation with T4 ligase (New England Biolabs, Ipswich, MA). 96 adapter-ligated samples were pooled and amplified to provide library quantities amenable for sequencing ...
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bioRxiv - Microbiology 2021Quote: ... The chimeric or mutated GBP sequences were then transferred into pAIP using restriction enzymes NotI and BamHI and the T4 DNA ligase (New England BioLabs). Transformed clones in E ...
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bioRxiv - Microbiology 2021Quote: ... Primers listed in supplemental table 1 were annealed and ligated into the digested plasmid using T4 ligase (New England Biolabs). For the generation of pGRA1.GFP.GRA2.DHFR ...
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bioRxiv - Bioengineering 2020Quote: ... was incubated with 2 μΜ biotinylated oligo complementary to one of the two 12 nt cohesive ends in λDNA in T4 DNA ligase reaction buffer (NEB) and hybridized at 70°C for 15 min followed by cooling to 15°C over 2 h ...
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bioRxiv - Biochemistry 2021Quote: ... The fragments were ligated into the pLSV101 vector (1:1 and 3:1 molar ratios) with T4 DNA ligase (New England Biolabs) (10 °C for 30 s and 30 °C for 30 s alternating overnight) ...
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bioRxiv - Neuroscience 2020Quote: ... either purified directly (insert) or from agarose gel (vector) and ligated (16-18 h, 20-22 °C) with T4 DNA ligase (NEB) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2021Quote: ... DNA was ligated by incubation at 16°C for 5h in 4 ml volume using 100U of T4 DNA ligase (NEB). After reverse crosslinking and Proteinase K treatment (Invitrogen) ...
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bioRxiv - Cancer Biology 2021Quote: ... the ligation of the custom sequence adapters was done in solution by adding 4 μl adaptors (30 μM) and 1600 units T4 DNA ligase (NEB). The ligation was carried at for 2 hours at room temperature on a rotating wheel in 1x ligation buffer (NEB) ...
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bioRxiv - Cancer Biology 2021Quote: ... and after stringent washing in the same buffer at 55°C for 10 min ligated to the custom SCRiBL adapter using 1600 units of T4 DNA ligase (NEB) for 2 hours at room temperature ...
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bioRxiv - Biophysics 2020Quote: ... along with the purified PCR fragment was digested with EcoRI and XbaI then ligated together with T4 DNA ligase (M0202, New England Biolabs). pENTR1a-3xNLS-mScarlet-I was then recombined using Gateway LR Clonase II (11791 ...
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bioRxiv - Cell Biology 2021Quote: ... pRS316 (Sikorski and Hieter, 1989), p424GPD, or p426GPD (Mumberg et al., 1995) through restriction enzyme digestion and T4 DNA ligase-mediated ligation (NEB). The resulting plasmids were verified by enzymatic digestion and DNA sequencing ...
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bioRxiv - Synthetic Biology 2021Quote: ... The annealing was carried out in 96-well PCR plates by mixing 2 μL of each oligonucleotide at 100 μM with 2 μL of 10x T4 DNA Ligase Reaction Buffer (NEB) and 14 μL of water ...
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bioRxiv - Synthetic Biology 2021Quote: ... the resulting DNA mixture could be directly used for following the ligation with the corresponding linear capture vectors by T4 DNA ligase (NEB).
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bioRxiv - Microbiology 2020Quote: ... The RNA fragments were ligated with 3’-barcoded adaptors at 22 °C for 1 h and 30 min using T4 RNA Ligase (NEB). Barcoded RNA samples were pooled together ...
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bioRxiv - Microbiology 2020Quote: ... RNA was fragmented via ultrasound (4 pulses a 30 sec, 4 °C) and subsequently treated with T4 Polynucleotide Kinase (NEB). Half of the samples were then treated with terminator exonuclease (TEX ...
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bioRxiv - Plant Biology 2021Quote: ... The 35S:RAM1 cassette from pK7WG2R was amplified using the primer pair listed in Table S1 and cloned into pK7GWIWG2(II)-RedRoot at the Kpn1 and Xho1 sites using T4 DNA ligase (NEB). The vectors were transformed into M ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA preparations were first 3’-dephosphorylated using T4 PNK for 1h at 37°C without ATP and pre-adenylated linker (Universal miRNA cloning linker, NEB) ligation was performed during 4h at 22°C in the presence of truncated RNA ligase 2 (NEB)30 ...
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bioRxiv - Molecular Biology 2020Quote: ... The vector and the PCR insert were used to prepare 4 ligation reactions by mixing with T4 Ligase (New England Biolabs).
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bioRxiv - Microbiology 2021Quote: ... The AMII sequencing adapter (Oxford Nanopore Technologies) was ligated to about 300 ng of barcoded pools using Quick T4 DNA ligase (New England Biolabs) and the library was purified using 0.5 volume of AMPure XP magnetic beads ...