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Citations for New England Biolabs :
3251 - 3300 of 10000+ citations for Thyroxine T4 ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Vector and fragment were ligated in an overnight reaction at 4 °C using T4 DNA Ligase (New England Biolabs). After transformation ...
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bioRxiv - Microbiology 2020Quote: ... The Flag-tagged spike gene was ligated to the linearized vector using the T4 DNA Ligase enzyme from NEB. The resulting plasmid ...
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bioRxiv - Microbiology 2021Quote: ... The reaction was heat-inactivated and DNA fragments were self-ligated using T4 DNA ligase (New England Biolabs, M0202T). E ...
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bioRxiv - Molecular Biology 2021Quote: ... before incubation during 6 min in 50μl of PNK reaction mix (1x PNKT, 1 mM ATP and 0.05 U/ml T4 PNK 3′phosphatase minus (NEB) in a thermomixer at 37°C and 1400rpm ...
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bioRxiv - Microbiology 2021Quote: ... Double digested insert and vector were subjected to ligation by a T4 DNA ligase in 10X ligation buffer (NEB) overnight at 16°C ...
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bioRxiv - Genetics 2020Quote: ... the suspension was then mixed with 200 μl of master mix containing 50 μl 10x T4 ligase buffer (NEB), 2.5 μl 20 mg/ml BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... The sgRNAs were then ligated with the digested plasmid using the T4 DNA ligase and buffer (New England Biolabs). Reactions were carried out following the Zhang Lab General Cloning Protocol (Addgene).
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bioRxiv - Cell Biology 2022Quote: ... to a final concentration of 0.5 μCi/μL and 0.8 U/μL T4 polynucleotide kinase (New England BioLabs, M0201S) for 30 min at 37°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... in which 20-50 ng of uncut acceptor plasmid and a 2:1 molar ratio of donor plasmid or PCR products were mixed together with 1.5 μl DNA T4 ligase buffer (#B0202S; NEB), 0.0015 mg BSA (B9000S ...
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bioRxiv - Genetics 2022Quote: ... Target sites were inserted by Golden Gate reaction using BsaI HF v02 and T4 DNA Ligase (New England Biolabs) per our previous description[48–50] ...
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bioRxiv - Bioengineering 2022Quote: ... custom synthesized oligonucleotides encoding the sgRNA spacer sequences (IDT) (Supplementary Table S1) were phosphorylated by T4 polynucleotide kinase (NEB) for 30 min at 37°C and subsequently annealed in a thermocycler (5 min at 95° ...
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bioRxiv - Microbiology 2021Quote: ... the THN gene was ligated to pICH41021 in a 3:1 molar ratio using T4 Ligase (New England Biolabs) at 4°C overnight and transformed by heat shock into chemically competent E ...
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bioRxiv - Microbiology 2022Quote: ... Digested pAd5-Blue and IE1 PCR products were then ligated using T4 DNA ligase (New England Biolabs, Ipswich, MA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Oligonucleotides complimentary to the target were end-labeled with γ-32P-ATP by T4 polynucleotide kinase (New England Biolabs). Labeled oligonucleotides were added to the membrane and incubated overnight at 45°C ...
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bioRxiv - Genetics 2022Quote: ... The digested PCR products and the pBSMVγ plasmid were ligated using T4 ligase (New England BioLabs, catalog number M0202). This plasmid was named pBSMVγQT1 (Figure 1b) ...
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bioRxiv - Genomics 2022Quote: ... The backbone and fragments of either miPSC_1_L1 or miPSC_4_L1 were combined in a single ligation reaction using T4 DNA Ligase (New England Biolabs, Cat#: M0202) and incubated overnight at 16°C ...
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bioRxiv - Biochemistry 2022Quote: ... End repair of RPFs RNA samples was conducted through dephosphorylation reaction—by adding 7.5 μL T4 polynucleotide kinase (PNK) 10X buffer (B0201S, NEB), 1.5 μL ATP (100 mM ...
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bioRxiv - Cancer Biology 2022Quote: ... Ligation of the 3’ adapter was done using the T4 RNA Ligase 1 (New England Biolabs, Ipswich, MA, M0204L). Ligation of 5’ adaptor required (i ...
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bioRxiv - Genomics 2019Quote: ... Samples were ligated overnight at 16ºC with a DNA / RNA oligo (rP5 _ RND: TTTCCCTACACGACGCTCTTCCGATrCrUrNrNrNrNrNrNrNrN) using T4 RNA ligase 1 (New England Biolabs). RNA integrity after ligation was assayed by agarose gel electrophoresis and poly(A)RNA was purigied using oligo dT magnetic beads ...
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bioRxiv - Bioengineering 2019Quote: ... and eluted in 8 µl 10 mM Tris pH 8.0 (EB) for ligation with 50 pmol of T-linker (see below) with T4 ligase (NEB) in 20 µl volume at 16 °C overnight ...
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bioRxiv - Immunology 2020Quote: ... Annealed and phosphorylated oligonucleotides were cloned into linearized and dephosphorylated BPK1520_puroR using T4 DNA Ligase (New England BioLabs M0202L) according to manufacturer recommendations ...
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bioRxiv - Molecular Biology 2021Quote: ... The 3’ terminus of total RNAs were ligated with the irCLIP adaptor by T4 RNA Ligase 1 (NEB, M0437M). After ligation ...
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bioRxiv - Pathology 2019Quote: ... sequencing adapters (ONT) were ligated onto the end-prepped library using NEBNext Quick T4 DNA Ligase (New England Biolabs) and bead purified (0.4× beads ratio ...
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bioRxiv - Synthetic Biology 2021Quote: ... 20 minutes at 80 °C followed by adding annealed inserts to vector DNA (0.020 pmol) at a 3:1 molar ratio with T4 DNA ligase (400 units; M0202S, New England Biolabs), T4 DNA ligase buffer and nuclease free water to a final volume of 20 μL for 30 minutes at room temperature and then 65 °C for 10 minutes.
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bioRxiv - Molecular Biology 2020Quote: ... in a total volume of 20 µL containing 10 U of T4 RNA Ligase 1 (NEB, cat n° M0204L), 1X T4 RNA ligase buffer ...
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bioRxiv - Systems Biology 2019Quote: ... the beads were incubated 16h at 16°C in 1xPNK buffer with 40U T4 RNA ligase I (NEB, M0204L), and 1 μl 100 μM L5 adapter (Supplementary Table 5) ...
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bioRxiv - Genomics 2020Quote: ... Precipitated DNA was incubated with adaptors at room temperature for 1 hr with T4 DNA ligase (New England Biolabs) and amplified with Illumina primers.
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bioRxiv - Genomics 2020Quote: ... Precipitated DNA was incubated with adaptors at room temperature for 1 h with T4 DNA ligase (New England Biolabs) and amplified with Illumina primers.
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bioRxiv - Synthetic Biology 2021Quote: ... modified and CE treated IVT RNA were ligated to ONT RT Adapter using concentrated T4 DNA ligase (NEB-M0202T), and was reverse transcribed using SuperScript III Reverse Transcriptase (Thermo Fisher Scientific-18080044) ...
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bioRxiv - Molecular Biology 2021Quote: ... The Taq I-digested chromatin was diluted 7-fold to which 10,000 cohesive end units of Quick T4 DNA ligase (New England Biolabs) were added ...
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bioRxiv - Developmental Biology 2019Quote: ... A ~300 bp fragment surrounding this site was PCR amplified with flanking BamHI restriction sites and cloned into the eGFP bait-E2A-KalTA4-pA plasmid49 by T4 ligation according to the manufacturer’s protocols (NEB), to produce the miR-223-E2A-KalTA4 bait plasmid ...
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bioRxiv - Genetics 2020Quote: ... and resuspended in 750 µl ligation mixture containing 200 units T4 DNA ligase (NEB/ IMB core facility protein production), 1X T4-Ligation buffer (NEB ...
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bioRxiv - Immunology 2021Quote: ... Complimentary oligonucleotides encoding the sgRNAs with suitable overhangs for ligation were annealed and phosphorylated using T4 Polynucleotide Kinase (NEB) with the T4 DNA Ligase Reaction buffer (NEB) ...
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bioRxiv - Biophysics 2019Quote: ... Purified DNA fragments were then ligated with 8000 units of T4 DNA ligase with 1mM ATP·MgCl2 (New England Biolabs) for 20 min at room temperature and purified again by native PAGE ...
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bioRxiv - Cell Biology 2021Quote: ... tissues were incubated at 37°C with 0.1 U/μl T4 DNA ligase (New England Biolabs, Ipswich, MA, USA) in 50mM Tris-HCl ...
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bioRxiv - Genetics 2021Quote: ... The Golden Gate Assembly reaction was set in 200µl PCR tubes (ThermoScientific AB2000) with 2.5 µl 10X T4 DNA ligase buffer (NEB B0202S), 0.5 µl T4 DNA ligase (NEB M0202L) ...
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bioRxiv - Immunology 2021Quote: ... tissues were incubated at 37°C with 0.1 U/µl T4 DNA ligase (New England Biolabs, Ipswich, MA, USA) in 50mM Tris-HCl ...
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bioRxiv - Molecular Biology 2021Quote: ... was cut from pS89 by NotI+BamHI and inserted into 50 ng NotI+BamHI-digested pBRa or pACλCI using T4 ligase (NEB) according to standard protocols ...
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bioRxiv - Microbiology 2021Quote: ... pcDNA3.1 HA-PARP12 was generated by PCR amplifying the PARP12 coding sequence (Dharmacon) and ligating into EcoRI/EcoRV sites of pcDNA3.1 using T4 DNA ligase (NEB). Construct sequence identity was confirmed by restriction enzyme digestion and Sanger sequencing (Genewiz) ...
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bioRxiv - Immunology 2021Quote: ... 5’ arm of homology to exon 5 of the Il22 gene was cloned into NotI-EcoRI-digested pMACs 4-IRES.II (contains EMCV IRES and truncated hCD4) using T4 DNA Ligase (NEB). Second ...
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bioRxiv - Molecular Biology 2021Quote: ... Oligonucleotide pairs were phosphorylated and annealed by mixing 100 pmol of each pair and 0.5 μL T4 PNK (NEB) then incubated at 37°C for 30 minutes ...
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bioRxiv - Genomics 2020Quote: ... PCR products with sticky ends were highly diluted to a final concentration less than 1ng/μl and ligated with a high concentration (66.68U/uL) of T4 DNA ligase (New England BioLabs) at 16°C for at least 16 hours ...
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bioRxiv - Genetics 2020Quote: ... the DNA was digested with the BsaI-HFv2 enzyme and a hairpin was ligated using T4 DNA ligase (NEB). The DNA was bisulfite converted using EZ DNA Methylation-Lightning kit (ZYMO RESEARCH ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Barcodes (4-8 bp) and common adapters were ligated with 400 U T4 DNA ligase (New England Biolabs Inc.) at 22 C for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... was cut from pS85 by NotI+BamHI and inserted into 50 ng NotI+BamHI-digested pBRa or pACλCI using T4 ligase (NEB) according to standard protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... was cut from pS89 by NotI+BamHI and inserted into 50 ng NotI+BamHI-digested pBRa or pACλCI using T4 ligase (NEB) according to standard protocols ...
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bioRxiv - Molecular Biology 2021Quote: ... was cut from pS85 by NotI+BamHI and inserted into 50 ng NotI+BamHI-digested pBRa or pACλCI using T4 ligase (NEB) according to standard protocols ...
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bioRxiv - Cancer Biology 2019Quote: ... Sheared and pulled down DNA was treated using a 100μl end-repair reaction (25mM dNTPs, 50U NEB PNK T4 Enzyme, 12U NEB T4 DNA polymerase ...
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bioRxiv - Cancer Biology 2022Quote: Purified PCR product was inserted into the pcDNA3.1 backbone using KpnI and NotI restriction endonucleases and T4 DNA Ligase (New England Biolabs). Correct cloning was verified by diagnostic restriction and direct sequencing (Microsynth ...
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bioRxiv - Bioengineering 2022Quote: ... the digested vector was ligated with a similarly digested DNA insert using a T4 DNA ligase (New England BioLabs). The preparation of t-NLuc under the lac promoter in the reconstructed pETDuet plasmid was achieved following a similar procedure but was double-digested with SgrAI and SacII restriction enzymes ...