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Citations for New England Biolabs :
2951 - 3000 of 10000+ citations for Thyroxine T4 ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Ligation to each splint was performed overnight at 16°C using T4 DNA ligase (New England Biolabs).
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bioRxiv - Developmental Biology 2020Quote: ... the nicks in the junction were sealed by ligation with T4 DNA Ligase (New England BioLabs, M0202T) to create a covalently closed circle ...
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bioRxiv - Developmental Biology 2020Quote: ... DNA-bound beads were incubated in 100 μl end-repair mix containing 1x T4 Ligase Buffer (NEB), 0.5 mM dNTP mix ...
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bioRxiv - Genomics 2020Quote: ... One microgram of the sonicated DNA was incubated in 1x T4 DNA ligase buffer (NEB; Ipswich, MA) with components ...
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bioRxiv - Genomics 2020Quote: ... 1 μg of the blunt-ended DNA was further incubated in 1x T4 DNA ligase buffer (NEB) in 1x T4 DNA ligase buffer (NEB ...
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bioRxiv - Genetics 2022Quote: ... gRNAs were cloned into the BbsI restriction sites of PX458 using T4 DNA ligase (New England Biolabs). gRNA expression cassette inserts (U6 RNA polymerase III ...
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bioRxiv - Molecular Biology 2021Quote: ... and the mix was ligated in 50 µL total volume with 1.5 µL T4 DNA Ligase (NEB) for 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by re-circularization through PNK phosphorylation of PCR product ends and ligation by T4 ligase (NEB). Similarly ...
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bioRxiv - Molecular Biology 2021Quote: ... Digested chromatin was then ligated at 16 °C with 10000 U of T4 DNA ligase (NEB, M0202) in ligase buffer supplemented with 10% Triton-100 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... 1μl of a 1:250 dilution of annealed gRNAs was ligated with the T4 DNA Ligase (NEB) into 36ng of the px459 V2.0 vector ...
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bioRxiv - Genomics 2022Quote: ... 7 μl PEG 8000 (17.5% final) and 1 μl of T4 RNA ligase 2 KQ (NEB, M0373L) in 1X T4 RNA ligase buffer ...
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bioRxiv - Genomics 2020Quote: ... 8.5 uL of this phosphorylated product was combined with 1 uL of 10X T4 ligase buffer (NEB) and 0.5 uL of T4 DNA ligase (NEB ...
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bioRxiv - Genomics 2020Quote: ... the material was blunted by the addition of 5μl of 3U/μl T4 DNA polymerase (NEB, USA) for 20 minutes at 16°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The beads were washed, treated with T4 Polynucleotide Kinase (PNK, with no 3’-phosphatase activity) (NEB, M0236S), and miRNA-RNA chimeras were produced with T4 RNA Ligase I (NEB ...
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bioRxiv - Microbiology 2019Quote: ... and double-stranded spacer was generated by phosphorylation with T4 PNK (New England BioLabs, Ipswich, MA, USA) and annealed at 95°C for three minutes ...
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bioRxiv - Genetics 2020Quote: ... Sense oligos diluted to 10 μM in nuclease-free water were phosphorylated with T4 PNK (NEB #M0201) supplemented with ATP ...
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bioRxiv - Microbiology 2019Quote: ... and phosphorylated for 30 minutes at 37 °C with 4U of T4 Polynucleotide Kinase (New England Biolabs) in a total volume of 20 µl according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Co-precipitated RNA was dephosphorylated and a 3’ RNA linker was ligated using T4 RNA ligase (NEB). The AATF-RNA complexes and input controls were run on a NuPage bis-tris protein gel ...
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bioRxiv - Synthetic Biology 2019Quote: ... DNA Polymerase I large (Klenow) fragment and T4 DNA ligase were purchased from New England BioLabs (NEB). Oligonucleotides and chemicals were obtained from Sigma-Aldrich ...
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bioRxiv - Genetics 2021Quote: ... Ligation buffer is a mixture with final concentrations of 1X T4 DNA Ligase Buffer + ATP (NEB M0202S), 0.01 % TritonX-100 ...
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bioRxiv - Biochemistry 2020Quote: ... 8 μL of the de-phosphorylated DNA was incubated with 2 μL T4 Polynucleotide Kinase (NEB M0203S), 3 μL γ32P-dATP (Perkin Elmer) ...
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bioRxiv - Biophysics 2021Quote: ... and 3 samples from each replicate combined into 7 mL with 1 × T4 DNA Ligase Buffer (NEB), with 1% Triton X-100 ...
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bioRxiv - Cancer Biology 2020Quote: ... The PCR product was then end-repaired to produce blunt ends using T4 DNA polymerase (NEB M0203L), DNA Polymerase I ...
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bioRxiv - Plant Biology 2021Quote: ... Nextera-compatible universal adapters [46] were ligated to the product (T4 DNA ligase; New England Biolabs, USA), and i5 and i7 PCR amplification was done (Q5 polymerase ...
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bioRxiv - Microbiology 2020Quote: ... The digested hemA PCR product and pHSG396 were ligated together using T4 ligase (New England Biolabs, US), at a 3:1 insert to vector mass ratio ...
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bioRxiv - Plant Biology 2021Quote: ... The asRNA genes were ligated into pLSU-1.1 with T4 DNA Ligase (New England Biolabs® Inc.) and the ligation products were transformed into Escherichia coli for colony PCR screening ...
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bioRxiv - Microbiology 2021Quote: ... Annealed oligos were gel-purified from 2% agarose gel and T7_2xS1m_SacI_BamHI DNA fragment was ligated with SacI+BamHI-digested pUC19 plasmid using T4 DNA ligase (NEB). The resulted plasmid was digested with BamHI and XbaI and ligated with 2xS1m_BamHI_XbaI dsDNA oligo to generate pUC19-T7-4xS1m construct ...
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bioRxiv - Biophysics 2020Quote: ... oligos listed in Table S2 were purchased from IDT and phosphorylated using T4 Polynucleotide Kinase (M0201L; NEB) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... The central fragment and two labelled fragments were then ligated by incubation with T4 DNA ligase (NEB) overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... In brief, size selected ribosome footprints were treated with T4 Polynucleotide Kinase (#M0201L, New England Biolabs (NEB)) followed by ligation to a DNA linker using T4 RNA ligase 2 ...
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bioRxiv - Molecular Biology 2022Quote: ... and tRNA-Gly-CCC (Table S1) were radioactively labeled with gamma-32P-ATP using T4 PNK (NEB). Pre-hybridization and hybridization were performed in OligoHyb Buffer (Applied Biosystems ...
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bioRxiv - Molecular Biology 2022Quote: ... and the L3 DNA linker (Table S1) was ligated to RNA by T4 RNA Ligase 2 (NEB). The beads were washed with high salt buffer and PNK buffer ...
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bioRxiv - Cell Biology 2022Quote: ... annealed and phosphorylated sgRNA (1:10) and T4 Ligase reaction buffer (1:10, #B0202S, New England Biolabs) with 10 mM 10x Adenosine 5’-Triphosphate (#P0756S ...
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bioRxiv - Plant Biology 2021Quote: ... The antisense strand with a 3′ phosphate was radiolabeled by T4 Polynucleotide Kinase (3’ phosphatase minus) (NEB) and [γ-32P]ATP ...
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bioRxiv - Microbiology 2020Quote: The 3′ ends of the ribosome footprint RNA fragments were then treated with T4 polynucleotide kinase (NEB) to allow ligation of a pre-adenylated DNA linker with T4 Rnl2(tr ...
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bioRxiv - Microbiology 2021Quote: ... The oligos were then ligated into the double digested psiCheck-2 plasmid using T4 DNA ligase (NEB) at room temperature for 2 h ...
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bioRxiv - Microbiology 2022Quote: ... For the noncapped assay the RNA in the reaction was treated with T4 PNK (NEB, Ipswich, MA) to produce a pool of 5’ monophosphate RNAs ...
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bioRxiv - Immunology 2022Quote: ... GTP was added to a final concentration of 2 mM along with T4 RNA ligase buffer (NEB). RNA was then heated at 55°C for 8 minutes ...
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Structure and mechanism of a Type III CRISPR defence DNA nuclease activated by cyclic oligoadenylatebioRxiv - Biochemistry 2019Quote: ... 10 μl eluted product was incubated with T4 DNA Ligase (New England BioLabs, Ipswich, MA, United States) in ligase buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genomics 2019Quote: ... Ligation was performed at a 1:3 ratio of pJDrcEPP:library using T4 DNA ligase (New England Biolabs). Ligation product was cleaned-up using a Clean and Concentrator Kit (Zymo Research ...
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bioRxiv - Developmental Biology 2020Quote: ... gRNAs were cloned into the digested U6.3>gRNA.f+e vector using T4 DNA Ligase (New England Biolabs) at room temperature for 20 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... Ligation and phosphorylation of PCR products were performed simultaneously using T4 DNA ligase (New England Biolabs, M0202L) and T4 Polynucleotide Kinase (New England Biolabs ...
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bioRxiv - Molecular Biology 2020Quote: ... New spacers were then cloned into the arrays by phosphorylation of oligo pairs with T4 PNK (NEB), hybridization of the oligo pair ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Klenow DNA polymerase and T4 PNK and incubated at room temperature for 30 minutes (NEB, Ipswich, MA) and then purified with the Qiagen QIAquick PCR purification kit (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by on-bead 3’RNA adapter ligation using high concentration T4 RNA Ligase I (NEB, #M0204L). IP efficiency was verified by immunoblot of 20% of the IP samples ...
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bioRxiv - Genomics 2021Quote: ... 10 ng A-tailed gel-isolated PCR product and 1 µl T4 DNA ligase (New England Biolabs) were mixed in a total reaction volume of 10 µl and incubated for 15 min at room temperature ...
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Efficient CRISPR/Cas9 genome editing in a salmonid fish cell line using a lentivirus delivery systembioRxiv - Genetics 2019Quote: ... 1 µL of each oligo (100 µM) were mixed with 1 µL of T4 ligation buffer (NEB) and 7 µL of water ...
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bioRxiv - Genomics 2020Quote: ... were assembled by hybridization and ligation over a complementary DNA strand using T4 RNA ligase 2 (NEB). To construct the hairpins ...
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bioRxiv - Genomics 2021Quote: ... 8.5 µL of this phosphorylated product was combined with 1 µL of 10X T4 ligase buffer (NEB) and 0.5 µL of T4 DNA ligase (NEB ...
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bioRxiv - Genetics 2020Quote: ... and pBac PUb-mCherry-SV40 digested with NotI and FseI were ligated with T4 DNA ligase (NEB) in a three-way ligation ...