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Citations for New England Biolabs :
251 - 300 of 2399 citations for Recombinant Rat Fc Fragment Of LgG Low Affinity IIb Receptor CD32 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... and rat (New England Biolabs, USA) that specifically depletes cytoplasmic (5S ...
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bioRxiv - Developmental Biology 2019Quote: Pceh-23_L::acy-2 fragment (sense/anti-sense) Pceh-23_L::acy-2 fragment(sense) was generated with a Gibson assembly cloning kit (NEB) by assembly of the following two DNA fragments ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids expressing DSB repair genes were constructed via Gibson assembly of gene fragment(s) and the vector fragment following the manufacturer’s instructions (New England Biolabs).
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bioRxiv - Genetics 2021Quote: ... The two fragments of jra along with the two fragments of the vector were ligated using Gibson assembly (NEB) and sequenced ...
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bioRxiv - Genomics 2023Quote: The standard HiDEF-seq v2 protocol was followed with the exception of replacing Klenow fragment 3’→5’ exo-polymerase with one of the following: 9.6 U Bst large fragment (NEB), 9.6 U Bst 2.0 (NEB) ...
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bioRxiv - Genomics 2021Quote: ... and 25 µl 2x NEBNext Hi-Fi PCR mix (NEB, USA) per reaction ...
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bioRxiv - Biochemistry 2021Quote: ... Nb35–His (10 μg/mL) and apyrase (25 mU/mL, NEB); the suspension was incubated for 1 h at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Nb35–His (10 µg/ml) and apyrase (25 mU/ml, NEB). The suspension was incubated for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: His-MBP-Fbp17SH3 was precipitated by amylose beads (NEB; cat#E8021L). After purification ...
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bioRxiv - Plant Biology 2022Quote: ... and MBP-HIS-GFP proteins were purified with amylose resin (NEB) and SUMO-HIS-BIN2 protein was purified with Ni-NTA Agarose (Qiagen).
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bioRxiv - Genetics 2023Quote: ... To facilitate homology directed assembly (Gibson or NEB Hi Fi assembly), 30bp homology with the preferred SEED cassettes were included at each site of the BsaI sites ...
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bioRxiv - Synthetic Biology 2023Quote: ... For his purpose pEKEx2 was first linearized using SacI (NEB, USA). The gene for mCherry was amplified by PCR using the primer pairs low_mCherry_fw and low_mCherry_rev (Table S1 ...
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bioRxiv - Cell Biology 2023Quote: ... For all Gibson Assemblies the NEB Hi-Fi Assembly Mastermix (NEB) was used ...
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bioRxiv - Neuroscience 2022Quote: ... Receptor cDNAs were amplified by PCR with Q5 High-Fidelity DNA Polymerase (New England Biolabs) from cDNA of mixed-stage populations of wild-type C ...
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bioRxiv - Neuroscience 2023Quote: ... 5-HT2A receptor mutants were designed and performed using Q5 mutagenesis kit (New England Biolabs). All DNA was sequence verified using Sanger nucleotide sequencing (Retrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... and was purified by affinity chromatography on amylose resin (NEB, Inc, USA) equilibrated with 20 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... Affinity tags were introduced by NEBuilder HiFi DNA Assembly Cloning Kit (NEB) into the expression vectors encoding Rae1 and Nup98 using primers in Table 1 ...
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bioRxiv - Biochemistry 2023Quote: ... and were first purified by affinity chromatography using the amylose resin (NEB). For untagged complex ...
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bioRxiv - Cell Biology 2023Quote: ... The eluate was loaded into the amylose affinity column (New England Biolabs) and washed with a buffer containing 30 mM Tris-HCl pH7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... the lysate was loaded into the cobalt affinity column (New England Biolabs) and eluted with a buffer containing 30 mM Tris-HCl pH7.5 ...
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bioRxiv - Cell Biology 2021Quote: Myc- and DDK (FLAG)-tagged Hemopexin mRNA was transcribed from an AgeI (NEB) linearised plasmid using T7 RNA polymerase (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... and tagged for multiplexing via the NE Next Multiplex Oligos for Illumina (NEB).
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bioRxiv - Molecular Biology 2022Quote: ... The HA-tagged PTBP1 was inserted into pcDNA3 between the EcoRI (NEB, R3101S) and NotI (NEB ...
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Nucleic acid sensing by STING induces an interferon-like antiviral response in a marine invertebratebioRxiv - Immunology 2022Quote: ... The MBP tagged proteins were purified by using Amylose Resin (New England Biolabs) and Ultrafiltration tubes (Millipore ...
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bioRxiv - Genomics 2021Quote: ... The purified recombinant protein or commercial M.EcoGII (NEB, M0603S) was diluted with coating buffer (0.05 M NaHCO3 buffer ...
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bioRxiv - Biochemistry 2021Quote: ... This experiment was further repeated with recombinant Histones (NEB) (0.5µg ...
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bioRxiv - Genetics 2020Quote: ... MEFs were protein transfected with recombinant RNase H (NEB) using Project Reagent Transfection Kit (Thermo ...
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bioRxiv - Molecular Biology 2023Quote: ... a combination of both recombinant RBPMS and BSA (NEB). Splicing reactions were incubated for the 3 hr ...
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bioRxiv - Molecular Biology 2023Quote: ... 20pmol of recombinant histone H3.1-H4 tetramer (NEB, M2509S) were immediately added to the tube ...
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A crucial role for dynamic expression of components encoding the negative arm of the circadian clockbioRxiv - Biochemistry 2023Quote: ... Recombinant human Histone H2A (New England BioLabs, Catalog # M2502S) or H4 (New England BioLabs ...
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bioRxiv - Developmental Biology 2021Quote: ... and mounted in 1% low melting point agarose (LMP) (Biolabs) within a 1mm diameter glass capillary ...
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bioRxiv - Microbiology 2020Quote: ... next to an RNA marker (low range ssRNA ladder; NEB) and run at 200 V for 45 minutes ...
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bioRxiv - Systems Biology 2020Quote: ... and desalted (Monarch low volume elution columns, New England Biolabs) for electroporation into Endura electrocompetent cells (Lucigen) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Low range ssRNA Ladder (New England BioLabs, Cat no N0364S) and 10 pmol of broccoli were run alongside the transcriptions as controls ...
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bioRxiv - Cell Biology 2022Quote: ... DNA fragments were end-repaired and T-tailed with Klenow fragment lacking 5’ → 3’ and 3’ → 5’ exonuclease activity (NEB). In-house adapters containing 8-nucleotide unique molecular identifier (UMI ...
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bioRxiv - Molecular Biology 2019Quote: ... Fragment ends were repaired using the NEBNext End Repair Module and adenosine was added at the 3’ ends of fragments using Klenow fragment (3’ to 5’ exo minus, New England Biolabs), universal adaptors were ligated to the A-tailed DNA fragments at room temperature for 1 h with T4 DNA ligase (New England Biolabs ...
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bioRxiv - Genetics 2022Quote: ... We produced linear DNA transformation fragments by digesting TFT-containing plasmids with SacI and BglII and gel purifying the fragments (Monarch Gel Purification, NEB). Genomic integration of each linear transformation fragment results in deletion of the LYP1 gene ...
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bioRxiv - Genetics 2022Quote: ... then the single nucleotide (A) was added to the end of the digestive fragment by Klenow fragment (3’-5’ exo-) (NEB) with dATP at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... The resulting PCR fragment was gel-purified and assembled with the gBlocks fragment using a 2x Gibson master mix (NEB). Gibson assembly was possible due to a 23 bp sequence shared between the PCR fragment and the gBlocks fragment ...
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bioRxiv - Microbiology 2023Quote: ... The larger fragment of the vector was gel purified as described above and the two fragments were ligated by T4 ligase (NEB) to generate the pLenti-NLuc1-9_IRES EGFP transfer plasmid ...
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bioRxiv - Molecular Biology 2023Quote: ... and the radiolabeled 3’ fragment was ligated to an unlabeled 5’ fragment by splinted ligation with T4 DNA ligase (NEB) at 30°C for 2 h ...
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bioRxiv - Neuroscience 2022Quote: ... Ribo-Zero Gold (Human/Mouse/Rat) Kit (Illumina; NEBNext® rRNA Depletion Kit (Human/Mouse/Rat)(E6350, NEB); NEXTflex™ Small RNA Sequencing Kit v3 (PerkinElmer ...
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bioRxiv - Cell Biology 2020Quote: ... PCR fragments were amplified using Q5 polymerase (NEB). Vectors were digested using enzymatic restriction digest and ligated to gel purified PCR products using Gibson assembly ...
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bioRxiv - Genomics 2021Quote: ... DNA Polymerase I Large Fragment (Klenow, NEB, M0210L), and T4 DNA ligase (Thermo Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... with 2.5 U of Klenow fragment exo-(NEB) at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... The telomeric fragments were released by ApaI (NEB) digestion and separated on 1.8% agarose gels ...
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bioRxiv - Molecular Biology 2020Quote: ... and A-tailed (Klenow fragment, New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... PCR fragments were amplified using Q5 polymerase (NEB). Vectors were digested using enzymatic restriction digest and ligated to gel purified PCR products using Gibson assembly ...
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bioRxiv - Genomics 2019Quote: ... large (Klenow) fragment 0.167U/μL (New England BioLabs) in CutSmart buffer (New England BioLabs ...
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bioRxiv - Microbiology 2019Quote: ... either the specific units of Klenow fragment (NEB) or the specified volumes of immunoprecipitated POLIC variants ...