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Citations for New England Biolabs :
401 - 450 of 2399 citations for Recombinant Rat Fc Fragment Of LgG Low Affinity IIb Receptor CD32 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Insert fragments were amplified with Q5 DNA polymerase (NEB) by PCR with the appropriate primer pairs listed on Table S3 ...
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bioRxiv - Systems Biology 2023Quote: ... Immunoprecipitated RNA fragments were then dephosphorylated (T4 PNK, NEB), polyadenylated ...
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bioRxiv - Cell Biology 2022Quote: ... The fragments were inserted into the AgeI (#R3552S, NEB) site of pTORA14 using the In-Fusion HD Cloning Kit,53 yielding pTORA14AAVS1 ...
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bioRxiv - Synthetic Biology 2023Quote: ... The amplified fragment was digested by DpnI (NEB #R0176) for an hour at 37°C ...
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bioRxiv - Neuroscience 2023Quote: Syt-1 fragments were produced in NEB-Express (NEB), while nanobodies were produced in SHuffle® Express (NEB) ...
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bioRxiv - Genetics 2023Quote: ... and DNA Polymerase I (Large Klenow Fragment; NEB #M0210) and ligation (with T4 DNA ligase ...
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bioRxiv - Neuroscience 2023Quote: ... Both fragments were digested using Gibson assembly (NEB E2611), yielding GFP7-11-CB5-mRuby3 (Suppl ...
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bioRxiv - Neuroscience 2023Quote: ... and assemble the fragments using Gibson assembly (NEB E2611) to generated GCaMP1-6 or GCaMP7-11 parts (Suppl ...
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bioRxiv - Molecular Biology 2023Quote: ... Eluted RNA fragments were dephosphorylated using T4 PNK (NEB), ligated to an adaptor using T4 RNA ligase II truncated KQ(NEB) ...
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bioRxiv - Neuroscience 2023Quote: ... Both fragments were digested using Gibson assembly (NEB E2611), resulting in TOM20-GFP1-6 (Suppl ...
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bioRxiv - Genetics 2023Quote: ... that was labelled using Klenow Fragment exo- (NEB, M0212L) with Digoxigenin-11-dUTP alkali-stable (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... The fragments were combined using T4 DNA ligase (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The fragments were tailed with dATP (New England Biolabs) by using Klenow Fragment (3′→5′ exo- ...
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bioRxiv - Developmental Biology 2024Quote: ... Fragments were digested using EcoRI-HF (New England Biolabs) and purified using the MinElute Cleanup kit (Qiagen) ...
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bioRxiv - Molecular Biology 2023Quote: The fragments were then treated with T5 Exonuclease (NEB) in a volume of 50 μL for 30 min at 37 °C to degrade all remaining linear ssDNA fragments ...
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bioRxiv - Biophysics 2023Quote: ... This fragment was then inserted into pUC19 (NEB N3041) to form pL14225 (a.k.a ...
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bioRxiv - Cancer Biology 2023Quote: ... NU-1611-Cy3),1.5ul of Klenow Fragment (3’→5′ exo-, NEB, M0212S) and 1.5ul of T4 Polynucleotide Kinase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... 8 μl 5 U/μL Klenow Fragment (NEB, M0210S) and 2 μl T4 PNK (NEB ...
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bioRxiv - Systems Biology 2024Quote: ... using DNA Polymerase I and Large (Klenow) Fragment (NEB). Blunt-ends were ligated with 4000 units T4 DNA Ligase and chromatin de-crosslinked using de-crosslinking buffer (50 mM Tris-HCl at pH 8.0 ...
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bioRxiv - Cancer Biology 2024Quote: ... The 601 DNA fragment was excised using EcoRV (NEB) and fragments further separated from the vector backbone using repeated steps of PEG precipitation.
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bioRxiv - Cancer Biology 2024Quote: ... 1.5ul of Klenow Fragment (3’→5′ exo-, NEB, M0212S) and 1.5ul of T4 Polynucleotide Kinase (NEB ...
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bioRxiv - Microbiology 2024Quote: ... DNA fragments were amplified using Phusion DNA polymerase (NEB) in reactions containing 10% DMSO and using GC buffer ...
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bioRxiv - Genomics 2021Quote: ... 25 μL NEBNext Hi-Fi 2x PCR Master Mix (NEB (Ipswich, MA, USA) M0541S) ...
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bioRxiv - Microbiology 2020Quote: ... and ligation with T4 ligase (Bioconcept) or by Hi-Fi Gibson assembly (NEB). PCR were performed using Phusion polymerase (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: His-SpCas9-GFP was expressed in and purified from BL21 (DE3, NEB, C2527H) bacteria as previously described 47 ...
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bioRxiv - Molecular Biology 2023Quote: Hi-C libraries were treated with 3 U of T4 DNA polymerase (NEB) in the presence of 0.1 mM dGTP (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and joined into the linearized vector by Hi-Fi Gibson DNA Assembly (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... The lysate was affinity purified using amylose resin (New England Biolabs, Ipswich, MA, USA). Wash buffer was 50 mM Tris pH 8.3 ...
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bioRxiv - Plant Biology 2021Quote: ... coli BL21(DE3) and purified by affinity chromatography using amylose resin (New England Biolabs). Recombinant His-UBA1 and His-UBC8 were purified using Ni-Ted resin (Macherey-Nagel) ...
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bioRxiv - Physiology 2023Quote: ... The proteins were then purified by affinity chromatography using amylose resin (Catalog E8021L, NEB). The amylose resin was washed and pre-equilibrated in 20 mM tris ...
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bioRxiv - Cell Biology 2020Quote: GST or GST-tagged SH3 domains were expressed in BL21 (DE3) E.coli (New England Biolabs). Cells were lysed by sonication in presence of lysozyme (Affymetrix) ...
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bioRxiv - Biochemistry 2021Quote: Pulldowns of MBP-tagged fusion proteins were performed using an amylose resin (New England Biolabs). Appropriate amounts of (see Fig ...
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bioRxiv - Genetics 2019Quote: ... Amplicons were tagged using NEB Next Ultra II DNA Library Prep Kit for Illumina (NEB) with 5 thermal cycles for amplification and subjected to paired-end sequencing on a MiSeq platform using MiSeq Reagent Nano Kit v2 ...
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bioRxiv - Cancer Biology 2020Quote: ... A 3’ A overhang was added to the ends of the blunted DNA fragment with Klenow Fragment (3’-5’ exo; NEB; Cat. No. M0212L). The DNA fragments was then ligated with diversity-increased custom barcodes (Shi et al. ...
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bioRxiv - Microbiology 2023Quote: ... each of the upstream and downstream fragments was ligated with the pKKPH fragment using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs, Japan, Tokyo, Japan). To insert pKKPH-p49 to the genome of ΔpyrFHM13 ...
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bioRxiv - Microbiology 2022Quote: ... were mixed with 2.5 µl Low Range ssRNA Ladder (New England Biolabs), 25 µl 2⨯ GLII loading buffer and 22.5 µl water and then denatured like the sample ...
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bioRxiv - Biophysics 2020Quote: Recombinant wild-type human histone H1.0 (New England Biolabs, cat. # M2501S) was used for experiments with fluorescently-labeled nucleosomes ...
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bioRxiv - Bioengineering 2021Quote: Recombinant RfxCas13d proteins were expressed in E.coli NiCo21(DE3) (NEB C2529). Cells were grown in 1L of lysogeny (Luria-Bertrani ...
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bioRxiv - Genetics 2019Quote: ... after the recombinant plasmids linearized with NotI Restriction Enzyme (NEB, USA), and then the capped mRNAs were purified by RNeasy Mini Kit (Qiagen ...
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bioRxiv - Immunology 2020Quote: ... and dephosphorylated using recombinant shrimp alkaline phosphatase (New England BioLabs M0371L). Annealed and phosphorylated oligonucleotides were cloned into linearized and dephosphorylated BPK1520_puroR using T4 DNA Ligase (New England BioLabs M0202L ...
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bioRxiv - Immunology 2021Quote: ... A similar dilution series of recombinant histone H3 (New England Biolabs) was prepared starting at 1 µg/ml protein ...
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bioRxiv - Biochemistry 2021Quote: Recombinant human CHIP was expressed in BL21(DE3) (New England Biolabs) E ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 5 mM DTT with 1.5 µM recombinant H3 (New England Biolabs), vehicle or 500 nM recombinant enzyme ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were induced in SHuffle Express strains (New England Biolabs) upon addition of 1mM IPTG in cultures containing kanamycin 50 μg/mL and incubated with agitation at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant bacmids were created by transformation into DH10Bac competent cells (NEB), screening on XGAL plates ...
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bioRxiv - Biochemistry 2024Quote: ... Protease Inhibitors) with recombinant PKA kinase (2500 U/mg, NEB-P600S) and 1 mM ATP overnight at 30°C and 250 rpm ...
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bioRxiv - Cell Biology 2024Quote: ... coli-derived recombinant human histone H1 (NEB, Ipswich, MA, USA #M2501), H2A (NEB ...
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bioRxiv - Plant Biology 2023Quote: ... Plasmids encoding chimeric SCS6/MLA1 and SCS6/MLA6 receptors were assembled using the NEBuilder HiFi assembly Kit (NEB) based on the domain boundaries reported in (28) ...
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bioRxiv - Microbiology 2020Quote: The plasmid pAAV S1-Fc was digested with New England Biolabs (NEB) Restriction Enzymes Pvu I-HF (Cat ...
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bioRxiv - Cell Biology 2020Quote: ... a 21 kb genomic DNA fragment of bacteriophage lambda (NEB) was flanked by a unique XbaI (position 0 ...