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Citations for New England Biolabs :
51 - 100 of 2399 citations for Recombinant Rat Fc Fragment Of LgG Low Affinity IIb Receptor CD32 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: A CD22 cDNA fragment encoding the first two Ig-like domains fused to an EK-hIgG-Fc fragment was amplified by PCR and cloned into the mammalian expression vector pACP-tag(m)-2 (New England Biolabs).
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bioRxiv - Microbiology 2022Quote: ... The purified fragments and the pABC3-His or pABC3-GFP vector were digested with PacI and NotI restriction enzymes (New England Biolabs, MA, USA). Resultant fragments were then gel-purified using the Monarch DNA Gel extraction kit (New England Biolabs ...
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bioRxiv - Plant Biology 2023Quote: ... MBP-tagged and GST-tagged proteins were detected using anti-MBP (E8032S, NEB) and anti-GST antibody (60-021 ...
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bioRxiv - Neuroscience 2022Quote: The three NMDAR fusion protein constructs were subcloned into pCSE2.7-mIgG2a-Fc-XP (N1-Fc and N2B-Fc) or pCSE2.8-mIgG2a-Fc-Xp (N1-N2B-Fc) using NcoI/NotI (New England Biolabs, Frankfurt, Germany) for mammalian production in Expi293F cells ...
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bioRxiv - Biophysics 2023Quote: ... Amylose affinity resin (NEB) was used for purification of fusion protein in batch which was eluted in buffer B (buffer A with 0.02% DDM ...
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bioRxiv - Microbiology 2023Quote: ... Libraries were selected for cDNA target fragments of 200 bp on 2% low-range Ultra agarose followed by PCR amplification using Phusion DNA polymerase (NEB, United States) for 15 PCR cycles ...
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bioRxiv - Microbiology 2019Quote: ... PCR fragments were blunted using Klenow fragment (NEB) and cloned into pUC57 and sequenced.
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bioRxiv - Molecular Biology 2021Quote: Biotinylation of SNAP tagged proteins and Avi-tagged proteins were performed as suggested by manufactures (NEB, Avidity). Direct biotinylation of proteins for example-YenB ...
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... Constructs expressing GFP-tagged or HA-tagged Shank3 phospho-mutants were generated using the Gibson Assembly kit (NEB) with the wild-type Shank3 as the template ...
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bioRxiv - Cell Biology 2021Quote: ... SNAP-tagged and Halo-tagged versions of TBK1 constructs were generated by inserting SNAP (pSNAPf [New England Biolabs]) or (Halo [pHaloTag vector ...
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bioRxiv - Microbiology 2022Quote: ... The glpFKD promoter fragments were labeled with 32P using DNA Polymerase I Large Fragment (Klenow fragment) (NEB) to fill in the 5’ overhang with [α-32P]dATP ...
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bioRxiv - Biochemistry 2019Quote: ... Large Fragment (NEB), 20-500 U/ml Nb.BssSI (NEB ...
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bioRxiv - Biochemistry 2021Quote: ... Klenow Fragment (NEB) (Figures 3C ...
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bioRxiv - Biochemistry 2019Quote: ... Large Fragment (NEB), 200 U/ml Nb.BssSI (NEB ...
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bioRxiv - Molecular Biology 2020Quote: ... + Klenow Fragment (NEB) + T4 polynucleotide kinase (NEB)) ...
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bioRxiv - Molecular Biology 2020Quote: ... Klenow Fragment (NEB) as in Chrzastek et al ...
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bioRxiv - Genomics 2022Quote: ... + Klenow Fragment (NEB) + T4 polynucleotide kinase (NEB)) ...
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bioRxiv - Microbiology 2023Quote: ... Klenow fragment (NEB) and adaptor ligated with annealed adaptors (Nimblegen Systems) ...
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bioRxiv - Genomics 2023Quote: ... Large Fragment (NEB), then incubating for 1 hour at 37 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... with Hi-Fi DNA builder (NEB). Primers used to amplify specific regions are described in (Supplementary Table 1) ...
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bioRxiv - Molecular Biology 2021Quote: ... Recombinant Cas9 protein (NEB) and sgRNA prepared by in vitro transcription were added to the mixture and incubated at 37℃ for ∼ 4h to remove rRNA ...
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bioRxiv - Biochemistry 2023Quote: ... purified by affinity chromatography (amylose resin (NEB) or Strep-Tactin XT resin (IBA) ...
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bioRxiv - Systems Biology 2019Quote: ... immunoprecipitated receptors were deglycosylated with PNGase F (New England Biolabs), before resuspending in LDS sample buffer and running on NuPAGE 4-12% Bis Tris precast gels ...
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bioRxiv - Molecular Biology 2020Quote: ... Exchange of V5/His to myc/His was performed using the Q5 site-directed mutagenesis kit (NEB #E0554) according to the manufacturer’s protocol resulting in pEF1-ZAP-S-myc/His and pEF1-ZAP-L-myc/His ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA fragment overhangs are filled with Klenow Fragment (3’-5’ exo-) (NEB) to leave an A-overhang ...
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bioRxiv - Molecular Biology 2021Quote: ... Klenow Fragment (NEB, M0210L) and 238.5 μl water ...
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bioRxiv - Molecular Biology 2019Quote: ... using Klenow fragment (NEB). Reactions were passed twice through a Sephadex G-25 desalting column (GE Healthcare ...
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bioRxiv - Cancer Biology 2020Quote: ... Large (Klenow) Fragment (NEB). The marked chromatin ends were ligated by adding 900µl of ligation master mix consisting of 663µl of water ...
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bioRxiv - Cancer Biology 2020Quote: ... Large (Klenow) Fragment (NEB).
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bioRxiv - Molecular Biology 2022Quote: ... Large (Klenow) Fragment (NEB).
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bioRxiv - Molecular Biology 2022Quote: ... Large (Klenow) Fragment (NEB). The marked chromatin ends were ligated by adding 900 µl of ligation master mix consisting of 663 µl of water ...
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bioRxiv - Cell Biology 2021Quote: ... Large (Klenow) Fragment (NEB) prior to ligation.
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bioRxiv - Cell Biology 2022Quote: ... Large (Klenow) fragment (NEB), 5 μl dCTP [α-32P] (3000Ci/mmol) ...
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bioRxiv - Cell Biology 2023Quote: ... Klenow Fragment (NEB # M0210S) to blunt the end ...
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bioRxiv - Molecular Biology 2023Quote: ... Large (Klenow) Fragment (NEB) in 40 µL reactions and incubated at 37 °C for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Large (Klenow) fragment (NEB) in a volume of 300.0 μL ...
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bioRxiv - Molecular Biology 2023Quote: ... Klenow fragment (NEB, M0210S) was added to the purified DNA for end-polishing at the final concentration of 1 U per microgram of DNA ...
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bioRxiv - Genomics 2023Quote: ... Klenow fragment (exo-) (NEB)
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bioRxiv - Genetics 2023Quote: ... Large (Klenow) Fragment (NEB) in 40 μL reactions and incubated at 37 °C for 30 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Large (Klenow) Fragment (NEB), in 1x NEBuffer3 ...
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bioRxiv - Cell Biology 2023Quote: ... Klenow Fragment (NEB # M0210S) to blunt the end ...
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bioRxiv - Genomics 2023Quote: ... Large (Klenow) fragment (NEB), incubated at 25 °C for 15 minutes ...
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bioRxiv - Genomics 2023Quote: ... using Klenow fragment (NEB) and re-ligated ...
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bioRxiv - Physiology 2024Quote: ... Klenow Fragment (M0212, NEB), and nuclease-free water ...
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bioRxiv - Biochemistry 2023Quote: ... large fragment (NEB, USA) and Exo I exonuclease (NEB ...
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bioRxiv - Immunology 2023Quote: ... via recombinant neuraminidase (NEB P0720L) as described by the manufacturer ...
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bioRxiv - Bioengineering 2021Quote: ... Each His or MBP fusion protein was purified through affinity chromatography with TALON metal affinity resin (Clonetech, Palo Alto, CA) or with amylose resin (New England Biolabs), respectively.
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bioRxiv - Biochemistry 2020Quote: Expressed proteins were purified using the intein-mediated purification and affinity chitin-binding tag (IMPACT) chitin affinity matrix system (New England Biolabs). A cell pellet was dissolved in 8 mL of column buffer (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2022Quote: ... The protein was purified first by immobilized metal affinity chromatography using PureCube Ni-NTA resin and then by affinity chromatography using amylose resin (NEB). N-terminal MBP fusion tag was removed by the cleavage with TEV protease (produced in-house) ...
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bioRxiv - Bioengineering 2021Quote: ... Standards (low range ssRNA ladder, NEB) and 10 pmol of unligated tRNA sample were prepared in an equivalent buffer to the first ligation reaction (1X RNA Ligase 2 buffer (NEB) ...