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Citations for New England Biolabs :
451 - 500 of 2399 citations for Recombinant Rat Fc Fragment Of LgG Low Affinity IIb Receptor CD32 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... This fragment was digested with NotI-HF and SmaI (NEB), while all other fragments were amplified by PCR using the Q5 HotStart High-Fidelity 2X master mix (NEB) ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA fragments were end repaired and dA-tailed (NEB #E7595), Illumina TruSeq adapters were ligated (NEB #E6040 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 0.5 μl of Bst large fragment polymerase (New England Biolabs) was added to each sample and the following thermal amplification regime is performed ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 3’ deoxy-adenine overhangs were added using Klenow Fragment (NEB), the sample was purified with QIAquick column ...
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bioRxiv - Neuroscience 2021Quote: ... The amplified PCR fragment was subcloned via HIFI cloning (NEB) under a Math1 enhancer.
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bioRxiv - Genetics 2020Quote: ... Fragments were then amplified with Q5 master mix (NEB, M0492L). Hi-C libraries were sequenced on the Illumina HiSeq X10 platform (PE 2×150 bp reads).
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bioRxiv - Microbiology 2019Quote: ... The resulting three fragments were digested together with BsmBI (NEB) for 1 hr at 55°C followed by overnight ligation at RT with T4 DNA ligase ...
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bioRxiv - Microbiology 2019Quote: ... The resulting fragment was digested with BamHI and XhoI (NEB) for 1 hr at 37°C followed by overnight RT ligation with similarly digested pPEPY-Plac plasmid and T4 DNA ligase (ThermoScientific) ...
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bioRxiv - Cell Biology 2019Quote: ... transposon fragments were amplified with Taq polymerase (New England Biolabs). The PCR products were then purified with the PCR clean-up/gel extraction kit (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2019Quote: ... the backbone fragment was treated with Shrimp Alkaline Phosphatase (NEB) prior to phosphorylation with γ-32P-ATP ...
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bioRxiv - Genomics 2021Quote: ... Adenylation was performed with 3’-5’ Klenow Fragment (NEB M0212L). Adaptors were ligated with NEB Quick Ligase for 10 minutes at 30°C before two rounds of cleanup with homemade beads ...
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bioRxiv - Microbiology 2021Quote: F(ab’)2 fragments were generated using IdeZ protease (NEB), purified using CaptureSelect LC-lambda affinity matrix (human ...
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bioRxiv - Neuroscience 2020Quote: ... DNA fragments were assembled using T5-exonuclease (New England Biolabs), Phusion DNA polymerase (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2021Quote: ... using a Klenow fragment (3′ → 5′ exo−) (New England Biolabs). Hybridization was performed at 65°C overnight in the pre-hybridization solution containing 6x saline-sodium citrate buffer ...
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Plant Trans-Golgi Network/Early Endosome pH regulation requires Cation Chloride Cotransporter (CCC1)bioRxiv - Plant Biology 2021Quote: ... Fragment ligation was performed using T4 DNA Ligase protocol (NEB) at 16°C overnight ...
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bioRxiv - Microbiology 2021Quote: ... the purified fragments were ligated into pMinit2.0 (New England Biolabs) and used for transformation of CaCl2-competent E ...
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bioRxiv - Molecular Biology 2021Quote: ... and 15 units of Klenow Fragment (3’→5’ exo-, NEB). Following extension reactions ...
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bioRxiv - Cell Biology 2022Quote: ... This fragment was digested with T7E1 Endonuclase1 (New England Biolabs) for 15 min at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... A-tailing (Klenow fragment (3’-5’ exo–, New England Biolabs), ligation to barcoded adapters (KAPA) ...
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bioRxiv - Microbiology 2022Quote: ... The obtained fragment was digested with XbaI (NEB, Cat# R0145) and NotI (NEB ...
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ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... The pCDH fragment was digested with DpnI (New England Biolabs) and was purified using QIAquick Gel Extraction Kit (QIAGEN) ...
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bioRxiv - Biophysics 2022Quote: ... The fragment was self-ligated with T4 DNA ligase (NEB) to create a 25.8kb linear DNA with a 48xlacO array and 3’-biotins at either end ...
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bioRxiv - Microbiology 2020Quote: ... RNA fragments were further dephosphorylated using T4 polynucleotide kinase (NEB) for 1h at 37°C followed by heat inactivation for 10min at 75°C ...
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bioRxiv - Genetics 2019Quote: ... These three fragments were PCR-amplified individually (New England Biolabs Q5 high-fidelity 2X master mix ...
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bioRxiv - Microbiology 2019Quote: ... The two fragments were joined together using Gibson reaction (NEB) and the resulting plasmid was transformed into E ...
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bioRxiv - Genomics 2020Quote: ... 3 mM DTT 8 μl Large Klenow Fragment (NEB, #M0210L) and 2 μl T4 PNK (NEB ...
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bioRxiv - Plant Biology 2021Quote: ... The 4 fragments were fused together using NEB builder (NEB). The reaction was amplified by PCR for 30 cycles and transformed into B ...
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bioRxiv - Biochemistry 2021Quote: ... The DNA fragments were next subjected to end repair (NEB) and dA-tailing adaptation ...
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bioRxiv - Genomics 2021Quote: ... Fragment ends were repaired using NEBNext End Prep kit (NEB) and AMX adapters were introduced using LSK109 ligation sequencing kit (Oxford Nanopore) ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 units of Klenow Fragment (3’à 5’ exo-, NEB), CutSmart buffer (NEB) ...
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bioRxiv - Developmental Biology 2021Quote: ... Fragments were amplified from 129S4 gDNA using Q5 polymerase (NEB) except for the middle segment of the long arm (i.e ...
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bioRxiv - Cell Biology 2022Quote: ... and second-strand synthesis performed with Klenow fragment (NEB M0212L) to enhance transcript recovery in the event of failed template switching80 ...
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bioRxiv - Microbiology 2022Quote: ... The fragment was subsequently ligated into the pMAL-c2X (NEB) expression vector via BamHI/SalI ...
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bioRxiv - Microbiology 2023Quote: ... Fragments were gel purified and enzymatically digested with SmlI (NEB) for 1h at 55 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Purified vectors and fragments were assembled using Gibson assembly (NEB) and constructs were transformed into competent cells ...
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bioRxiv - Molecular Biology 2023Quote: ... and sgRNA fragments were isolated by SbfI (New England Biolabs) restriction digestion and Ampure bead size selection then amplified by PCR for deep sequencing as described in 53 ...
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bioRxiv - Genomics 2023Quote: ... and 7.5 U of Klenow fragment 3’→5’ exo-(NEB), except for input DNA that is degraded (e.g. ...
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bioRxiv - Immunology 2023Quote: ... and second-strand synthesis performed with Klenow fragment (NEB M0212L) to enhance transcript recovery in the event of failed template switching 14 ...
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bioRxiv - Plant Biology 2023Quote: ... The PCR fragments were cloned into the miniT vector (NEB), and assembled together in a Golden Gate reaction together with the terminator tHSP18.2 (Addgene GB0035 ...
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bioRxiv - Genetics 2023Quote: ... DNA fragments were amplified using Q5 DNA polymerase (NEB 0491). PCR products were purified using the Monarch PCR & DNA Cleanup Kit (NEB T1030) ...
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bioRxiv - Molecular Biology 2023Quote: ... before assembly with fragments using NEBuilder HiFi DNA Assembly (NEB) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... This fragment was inserted by Gibson assembly (New England Biolabs) into Eps15-pmCherryN1 (Addgene plasmid#27696 ...
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bioRxiv - Genetics 2023Quote: ... All fragments were assembled into PCR linearized pUC19 vector (NEB) to give the final plasmid pZCS36.
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bioRxiv - Bioengineering 2023Quote: ... both fragments were combined via Gibson assembly (New England Biolabs), and the resulting TfRΔecto-RFP-mSA clone was verified by sequencing.
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bioRxiv - Developmental Biology 2023Quote: The amplified fragments were assembled using Gibson Assembly kit (NEB) and verified by Sanger sequencing (Genewiz) ...
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bioRxiv - Microbiology 2022Quote: ... 2.5 μL of Klenow Fragment (3’ -> 5’ exo -, NEB M0212L), 5 μL 50% PEG 8000 ...
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bioRxiv - Microbiology 2023Quote: ... and assembling the fragments by NEBuilder HiFi DNA Assembly (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... using Large Klenow fragment 3’-5’ exo- (New England Biolabs). Biotinylated 19x 601 array DNA ...
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bioRxiv - Genomics 2023Quote: ... by using Klenow Fragment (3′→5′ exo-) (New England Biolabs) for 30 min at 37 °C and purified by using a QIAquick PCR Purification Kit ...
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bioRxiv - Molecular Biology 2023Quote: ... A-tailing by Klenow Fragment (3’è5’ exo-; NEB M0212S), and TruSeq 6-bp index adaptor ligation by Quick ligase (NEB M2200S) ...