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Citations for New England Biolabs :
251 - 300 of 10000+ citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... pre-existing SNAP-tagged histones were first quenched by incubating cells with 10 μM of the non-fluorescent SNAP reagent (SNAPcell Block, New England Biolabs) for 30 min at 37°C followed by a 30 min wash in fresh medium and a 2 h chase ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were grown on glass coverslips and pre-existing SNAP-tagged histones were first quenched by incubating cells with 10 µM of the non-fluorescent substrate SNAP-cell Block (New England Biolabs) for 30 min followed by a 30 min-wash in fresh medium and a 2 hr-chase ...
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bioRxiv - Molecular Biology 2020Quote: ... The new SNAP-tagged histones synthesized during the chase were fluorescently labelled with 2 µM of the red-fluorescent reagent SNAP-cell TMR star or SiR-647 (New England Biolabs) during a 15 min-pulse step followed by 30 min wash in fresh medium ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescent probes diluted in Hybridization Buffer (10% dextran sulfate, 0.1mg/ml salmon sperm ssDNA, 100 μl vanadyl ribonucleoside (NEB biolabs), 20ug/ml RNAse-free BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... The new SNAP-tagged histones synthesized during the chase were fluorescently labelled with 4 μM of the green-fluorescent reagent SNAP-cell Oregon green (New England Biolabs) during a 15-min pulse step followed by 30-min wash in fresh medium ...
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bioRxiv - Immunology 2022Quote: ... was fused to the N-terminus of the fluorescent gene mTurquoise2 via a linker sequence (encoding MTSKSGGGGSGGGG) and cloned using NEBuilder Hifi DNA assembly (New England Biolabs) into a dual promoter lentiviral vector co-expressing a puromycin resistance gene ...
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bioRxiv - Molecular Biology 2023Quote: ... pre-existing SNAP-tagged histones were first quenched by incubating cells with 10 μM of the non-fluorescent SNAP reagent (SNAP-cell Block, New England Biolabs) for 30 min at 37°C followed by a 30 min wash in fresh medium and a 2-hour chase ...
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bioRxiv - Evolutionary Biology 2022Quote: ... melanogaster strain to be injected and inserted into plasmids containing fluorescent eye markers using NEBuilder Hi-Fi DNA assembly (NEB). See key resources table for primers sequences and donor plasmids.
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bioRxiv - Molecular Biology 2023Quote: ... The SNAP-tagged histones neosynthesized during the chase time were then pulse-labelled by incubating cells with 2 μM of the red-fluorescent SNAP reagent SNAP-cell TMR star (New England Biolabs) for 15 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... pre-existing SNAP-tagged histones were quenched during 30 min with 10 µM of the non-fluorescent reagent SNAP-cell Block (New England Biolabs), followed by a 30-min wash in fresh medium and a 2-h chase ...
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bioRxiv - Synthetic Biology 2023Quote: ... was then done with the homologies to construct the initial green fluorescent protein (GFP) plasmid and then transformed into competent E.coli (NEB#C2984H) and subsequently sequenced (Plasmidsaurus ...
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bioRxiv - Biochemistry 2023Quote: ... The array DNA was composed of a fluorescent nucleotide Alexa Fluor 647-aha-dCTP (ThermoScientific) and was generated using Klenow Fragment 3’ – 5’ exo- (NEB). Nucleosome arrays were mixed with SUV420H1 or 5μM HP1α and incubated at room temperature for 20 mins before transferring to a glass bottom 384 well plate for imaging ...
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bioRxiv - Molecular Biology 2023Quote: Total SNAP-tagged histones were labeled by incubating cells with 2 µM of the red-fluorescent reagent SNAP-cell TMR-star (New England Biolabs) for 15 min before cell fixation ...
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bioRxiv - Developmental Biology 2023Quote: Whole 72hpf animals electroporated with Kaede::nls containing constructs for lineage tracing analysis were converted using UV (405 nM) light from either a fluorescent microscope or the OptoWELL system (Opto Biolabs). Kaede conversion took 5-10 seconds per juvenile on the microscope or 1 minute on the OptoWELL for all juveniles at once ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was PCR amplified (primers hFUR Exon 1-3 PCR For and hFUR Exon 1-3 PCR Rev) and purified (Monarch PCR & DNA cleanup kit, NEB). The resulting PCR product was sequenced using the hFur Exon 1 Seq primer.
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bioRxiv - Neuroscience 2021Quote: ... Donor plasmids for homology directed repair were generated by cloning homology arms from Canton-S genomic DNA with Phusion Hot Start DNA polymerase (New England Biolabs) and inserting them sequentially into the pHD-DsRed-attP vector using restriction enzymes 55 ...
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bioRxiv - Molecular Biology 2021Quote: ... Restriction digestions were performed in a 60 ml volume with 1200 ng of DNA and 4–4.5 ml restriction enzyme(s) (Chr1 hotspot pair: NsiI, NcoI, and BglII or NcoI alone; Chr19 hotspot pair: BamHI or KpnI; BioLabs). Reactions were incubated overnight at 37 °C followed by an additional 3 h of incubation with a 1.5–2 ml of fresh restriction enzyme(s) ...
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bioRxiv - Genomics 2019Quote: ... NEBNext Universal PCR primer for Illumina (5’-AAT GAT ACG GCG ACC ACC GAG ATC TAC ACT CTT TCC CTA CAC GAC GCT CTT CCG ATC-s-T-3’) and NEBNext Index primer for Illumina (NEB, index# 9-13 for five libraries in each repeat) ...
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bioRxiv - Physiology 2020Quote: ... and the Esp3I fragment of the pPVxRF3 vector (a gift from S. Kondo) containing Venus and 3xP3-dsRed-Express2 using NEBuilder HiFi DNA Assembly Master Mix (NEB). The combined fragment was cloned into pBluescript ...
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bioRxiv - Genomics 2021Quote: ... Methylation reactions were performed in a 100uL reaction containing 1X CutSmart Buffer and 1mM S-adenosyl-methionine (SAM, New England Biolabs) and incubated at 37°C for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Either the wild-type or the mutant version of the PRNP 3’U R were inserted into the digested pmirGLO vector by performing a Gibson Assembly following manufacturer`s instructions (NEB). After transformation of the cloned vectors and purification of the DNA using EndoFree Plasmid Maxi Kit (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... The D614G S-protein was generated by introducing the mutation into the Wuhan reference strain via Q5 Site-directed mutagenesis (NEB). Other individual mutations were subsequently introduced into the D614G S by the same process ...
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bioRxiv - Biochemistry 2020Quote: Deglycosylation of S protein from the lysate of HEK293 cells transfected with pTwist-EF1a-nCoV-2019-S-2xStrep plasmid was performed as described previously [35] using PNGase F (New England Biolabs).
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bioRxiv - Bioengineering 2022Quote: ... In vitro Cas9 cleavage assay was carried out using purified IVT product and Cas9 protein (Cas9 Nuclease, S. pyogenes, New England BioLabs) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Capillary tubes (0.025⍰mm inner diameter) were filled with either 0.5⍰mM hemin or 0.5⍰mM S-adenosylmethionine (SAM) (New England Biolabs, Ipswich, MA) in the motility buffer and sealed with vacuum silicone grease (Dow Corning ...
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bioRxiv - Molecular Biology 2021Quote: ... 95°C/15 s and annealing/extension: 65°C/5 min) of PCR using Q5 high-fidelity DNA polymerase (NEB). Amplicons were purified using 1× AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Genomics 2020Quote: ... Reactions were performed in a 200uL reaction with 1X CutSmart Buffer and 1mM S-adenosyl-methionine (SAM, New England Biolabs) and incubated with 2.5uL enzyme at 37°C for 30 minutes ...
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The plant pathogen Pectobacterium atrosepticum contains a functional formate hydrogenlyase-2 complexbioRxiv - Microbiology 2019Quote: ... of the target gene(s) was amplified and inserted into pKNG101 using a three fragment Gibson assembly reaction (HiFi Assembly, NEB). For the insertion of a deca-His encoding sequence into hyfG ...
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bioRxiv - Physiology 2021Quote: ... and the Esp3I fragment of the pPVxRF3 vector (a gift from S. Kondo) containing Venus and 3xP3-dsRed-Express2 using NEBuilder HiFi DNA Assembly Master Mix (NEB). The combined fragment was cloned into pBluescript ...
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bioRxiv - Genomics 2021Quote: ... 95°C/15 s and annealing/extension: 65°C/5 min) of PCR using Q5 high-fidelity DNA polymerase (NEB). Amplicons were purified using 1× AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Genomics 2022Quote: ... Dam activity was induced by resuspension in 100 μL DigWash supplemented with 80 μM S-adenosyl-methionine (SAM) (NEB, B9003S) and incubated at 37 °C for 30 minutes ...
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bioRxiv - Bioengineering 2022Quote: 200 nM of isolated smgRNA were injected in a pre-incubating solution of 200 nM Cas9 (S. pyogenes, NEB, M0386), 120 nM fluorescent beacon and 1 U.μL-1 murine RNase Inhibitor (NEB M0314 ...
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bioRxiv - Plant Biology 2023Quote: ... At least 50 μg of total RNA was randomly fragmented for 125 s at 90°C to approximately 200 nt using the NEBNext Magnesium RNA Fragmentation Module (New England Biolabs). Fragmented RNA was precipitated with 4 μl of GlycoBlue (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... Mutagenic PCR constructs were generated by amplifying the upstream and downstream DNA fragments flanking the gene(s) of interest followed by fusion of these fragments with the Janus Cassette using a HiFi assembly master mix (NEB). Transformation of TIGR4 with the mutagenic construct (100ng/ml ...
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bioRxiv - Microbiology 2023Quote: ... concisus for 2 h at 37°C in presence of 0.4 mM S-Adenosylmethionine (SAM, New England Biolabs, Ipswich, MA). After methylation ...
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bioRxiv - Biochemistry 2023Quote: ... The same construct containing a blasticidin-S deaminase (BSD) gene in place of PAC was generated by Gibson assembly (NEB) using primers ZJ1-ZJ4 (Table S2) ...
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bioRxiv - Neuroscience 2021Quote: Libraries were prepared from 1 ug RNA using a library prep kit (NEB #7770), rRNA depletion kit (NEB #E6310) ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg RNA was reverse transcribed to cDNA using LunaScript RT SuperMix Kit (NEB). qPCR was performed in 20 μl reactions containing diluted cDNA ...
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bioRxiv - Genetics 2024Quote: ... immunoprecipitated RNA and 1% input RNA Monarch RNA Cleanup Kit (New England Biolabs, T2047L) was used according to the manufacturers’ instructions ...
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bioRxiv - Biochemistry 2019Quote: ... Three replicates of each condition were subjected to fluorescent PCR with a FAM-labelled forward primer (below) and with Phusion polymerase (NEB #M0530S) for 32 cycles ...
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bioRxiv - Cell Biology 2022Quote: ... were incubated with 25 μL of containing TF or 18S primers and fluorescent Luna® Universal qPCR Master Mix (New England Biolabs), and qRT-PCR was carried out in triplicate for each sample on the CFX Connect real-time System (Bio-Rad Laboratories).
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Genomic local adaptation of a generalist plant species to pollinator communities and abiotic factorsbioRxiv - Plant Biology 2022Quote: ... The nicked DNA was labelled with a fluorescent-dUTP nucleotide analogue using Taq DNA polymerase (New England BioLabs, cat. no. M0267S). After labelling ...
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bioRxiv - Genomics 2021Quote: ... gDNA was extracted from 1 ml of overnight culture with the kit Monarch HMW DNA Extraction kit (T#3060 New England Biolabs; Ipswich, MA, USA) following the manufacturer instructions for High Molecular Weight DNA extraction from gram-negative bacteria ...
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bioRxiv - Genetics 2020Quote: ... During the experiment a fresh aliquot with 46 ng/μl sgRNA and 3.13 μM commercial Cas9 enzyme (Cas9 Nuclease, S. pyogenes, 20 μM; NEB, Ipswich, USA) was used for each day and stored on ice.
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bioRxiv - Biochemistry 2020Quote: ... UK) for the presence of the correct mutation(s) and the cassette for integration digested out with AscI (NEB, Hitchin, UK) prior to transfection.
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bioRxiv - Molecular Biology 2021Quote: ... The G1 and S/G2 population were sorted using FACSAria II cell sorter (Becton Dickinson) in PBS supplemented with RNase inhibitor (NEB, M0314L). Sorted samples were processed for scRNA-seq or cDNA-seq.
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bioRxiv - Genomics 2023Quote: Nuclei were resuspended in 200 uL of Methylation Reaction Buffer (Buffer M containing 1mM S-adenosylmethionine (SAM)) (New England BioLabs B9003S). 10uL high-concentration EcoGII was added per 1e6 nuclei and the nuclei suspension was incubated at 37°C for 30 minutes ...
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bioRxiv - Genomics 2024Quote: ... The genomic DNA was incubated in a mix containing S-Adenosyl methionine (SAM) along with the DNA adenine Methyltransferase (dam, NEB, USA) and its corresponding buffer for 4 hours at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... the purified cDNA was PCR amplified and barcoded using ONT’s PCR Barcoding Expansion 1-12 (EXP-PBC001) or PCR Barcoding Expansion 1-96 (EXP-PBC096) kits with LongAmp Taq 2x Mastermix (NEB, Ipswich, MA).
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bioRxiv - Genomics 2019Quote: ... expressing ATG7 isoform 2 was a gift from Toren Finkel (Addgene plasmid #24921).31 The plasmid pCMV-myc-Atg7(1) expressing ATG7 isoform 1 was derived from pCMV-myc-Atg7(2) using the Q5 site-directed mutagenesis kit (NEB, Ipswich, MA). Subsequently ...