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Citations for New England Biolabs :
101 - 150 of 10000+ citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... 1 μL of this was then combined with 5 μL Q5 buffer (NEB, cat#M0491 S), 0.5 μL 10mM DNTP (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: One microgram of full-length S protein was treated with 1 μg of FXa (P8010L, NEB), furin (P8077S ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescent cell permeable Snap-Cell substrates (NEB) were used in the following concentrations ...
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bioRxiv - Plant Biology 2020Quote: ... MBP and GST fusion proteins were separated from supernatants using amylose resin (New England Biolabs) and Glutathione Sepharose 4 Fast Flow (GE Healthcare Life Sciences) ...
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bioRxiv - Molecular Biology 2021Quote: Myc-Tag (9B11) antibody (NEB 2276 S) and mouse IgG (Santa Cruz sc-2025 ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 µL 32mM S-Adenosyl methionine (NEB) (final concentration 0.8 mM) ...
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bioRxiv - Genomics 2021Quote: ... 0.2 mM S-adenosylmethionine (SAM) (NEB, #B9003) in 60 μL total volume for 30 min at 37 ºC ...
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bioRxiv - Genetics 2021Quote: ... 80 μM S-adenosylmethionine (New England Biolabs), and 40 units of EcoRI methyltransferase (New England Biolabs) ...
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bioRxiv - Developmental Biology 2022Quote: ... 160 μM S-adenosylmethionine (NEB, Cat# B9003S), 1 U/μl RNasin (Promega ...
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bioRxiv - Bioengineering 2022Quote: ... 200 nM Cas9 (S. pyogenes, NEB, M0368), 120 nM fluorescent beacon ...
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bioRxiv - Microbiology 2023Quote: ... containing 160 μM S-adenosylmethionine (SAM: NEB). This five-enzyme methylation cocktail was previously necessary to achieve genetic alteration in the strain as described in Umaña et al14.
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bioRxiv - Genomics 2023Quote: ... and SalI-HF enzyme (NEB; r3138 S) digested hPGK-STARR-seq vector ...
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bioRxiv - Microbiology 2021Quote: ... pCMV-hnCoV-S-H501Y-Δ69/70 was obtained from pCMV-7.1-hnCoV-S-H501Y (forward: 5’-TCCGGCACAAACGGCACA-3’, reverse: 5’-GATGGCGTGGAACCATGTC-3’) via Q5 SiteDirected Mutagenesis Kit (NEB). All plasmids were confirmed by gene sequencing (BGI Beijing) ...
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bioRxiv - Biochemistry 2021Quote: ... with and without the addition of ACE2 (~1:1.25 S-protein trimer:ACE2 molar ratio) (New England Biolabs). Vitrified samples of S-protein constructs with and without ACE2 were prepared by first glow discharging Quantifoil R1.2/1.3 300 mesh holey carbon copper grids for 1 minute using a Pelco easiGlow glow discharge unit (Ted Pella ...
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bioRxiv - Genomics 2023Quote: ... 1 μl of Hia5 MTase (200 U)19 and 1.5 μl 32 mM S-adenosylmethionine (NEB B9003S) (0.8 mM final concentration ...
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bioRxiv - Microbiology 2019Quote: ... Then deoxycytosine homopolymer tail (C-tail) was added to the linear extension purified PCR product using Terminal Transferase (TdT, New England Biolabs, Ipswich, MA, USA) enzyme following previous protocol (Lazinski and Camilli ...
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bioRxiv - Molecular Biology 2023Quote: ... digoxigenin-11-ddUTP was added to the 3’ end of the digested fragments using terminal transferase enzyme (New England Biolabs, Ipswich, MA, USA).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were labelled with the cleavable SNAP-tag probe BG-S-S-549 (a gift from New England Biolabs) in complete medium for 30 min at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... 20 s annealing at 63 °C and 30 s elongation by Q5 High-Fidelity DNA Polymerase (New England Biolabs) at 72 °C ...
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bioRxiv - Bioengineering 2020Quote: ... we included LAMP Fluorescent Dye (New England Biolabs) as a general LAMP indicator ...
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bioRxiv - Cell Biology 2019Quote: A construct expressing SURF4 and the Katushka2S fluorescent marker (PGK-SURF4-p2A-Katushka2S) was assembled with the NEBuilder HiFi DNA assembly cloning kit (NEB) using vector sequence derived from LV1-5 (Addgene #68411 ...
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bioRxiv - Molecular Biology 2023Quote: ... by replacing the green fluorescent protein (GFP) with a mCherry reporter using the NEBuilding HiFi DNA Assembly Cloning Kit (New England Biolabs) (Ran et al. ...
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bioRxiv - Cell Biology 2020Quote: Purified GST-Bbs5 protein (400 μg) was incubated with 6 μl of PKC (New England Biolabs) and 10 μl of ATP (New England Biolabs ...
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bioRxiv - Immunology 2021Quote: ... pSFG-SARS-CoV-2 S D614G was cloned from pSFG-SARS-CoV-2 S plasmid using the Q5 Site-Directed Mutagenesis Kit (New England BioLabs). 293T cells were transfected with 3.75 μg pSFG-SARS-CoV-2 S or pSFG-SARS-CoV-2 S D614G ...
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bioRxiv - Microbiology 2020Quote: ... pDWV-S-GFP and the parental pDWV-304 were produced using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: SARS-CoV-2 S N501Y plasmid was obtained from SARS-CoV-2 S plasmid (HDM-IDTSpike-fixK) by site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs). SARS-CoV-2 S and SARS_CoV-2 S N501Y pseudotyped retroviral particles were produced in HEK293T cells as described previously (29) ...
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bioRxiv - Microbiology 2020Quote: The SARS2-CoV-2 vaccine candidates were cloned by combining the S cDNA (obtained after PCR on overlapping synthetic cDNA fragments; IDT) by a NEB Builder Cloning kit (New England Biolabs) into the pShuttle-YF17D backbone ...
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bioRxiv - Microbiology 2021Quote: ... reverse: 5’-GATGGCGTGGAACCATGTC-3’) were obtained from the wild type plasmids pCMV-hnCoV-S via Q5 SiteDirected Mutagenesis Kit (NEB). pCMV-hnCoV-S-H501Y-Δ69/70 was obtained from pCMV-7.1-hnCoV-S-H501Y (forward ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR fragment was gel purified and inserted into plasmids pTRC99a and pKI_IS10 (gift of S. Wenk) using HiFi DNA Assembly Cloning Kit (NEB. pZE21) for Gibson cloning ...
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bioRxiv - Cell Biology 2021Quote: MBP-M18BP1.S-161-580 and MBP-M18BP1.S-161-580 SANTA were independently expressed from modified pMal-c2x plasmids (NEB) in BL21 (DE3 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were labelled with the cleavable SNAP-tag probe BG-S-S-649 (featuring the DY-649 fluorophore, a gift from New England Biolabs) in complete medium for 30 minutes at room temperature ...
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bioRxiv - Biophysics 2022Quote: ... coli BL21 (DE3)-pLyse S (NEB, Catalogue # C3010I) cells ...
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bioRxiv - Molecular Biology 2019Quote: ... 0.1 mM S-adenosylmethionine (SAM, New England Biolabs), 2 u/µl NxGen RNase inhibitor (Lucigen) ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 mM S-adenosylmethionine (SAM, NEB, Ipswich, MA), 0.5 U/μl RNasin (Thermo Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1 mM S-adenosylmethionine (SAM, New England Biolabs), 1 u/µl Murine RNase inhibitor (Vazyme) ...
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bioRxiv - Plant Biology 2022Quote: ... oligo(dT)s and Murine RNAse inhibitor (NEB) in a 20 μL reaction volume according to manufacturer instructions ...
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bioRxiv - Cell Biology 2023Quote: ... and 0.5 µg/µl BSA (NEB, B9000 S). Immunoprecipitations were performed at 4°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... 0.3 µM fluorescent SNAP substrate (TMR Star, NEB, S9105) was added along with the primary antibodies
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... GST-GGA3 was grown overnight in 50 mL of Laria-Bertani broth in NiCo21 E Coli (NEB). The following day the overnight culture was transferred to 1L of terrific buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The His-GST tags were cleaved by incubating the pooled fractions with TEV protease (New England Biolabs) at 4°C for 16h ...
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bioRxiv - Cancer Biology 2021Quote: ... 50 μg EBNA2 or 130 μg GST-fused EBNA2 protein were incubate in 1x PK buffer (NEB, 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2023Quote: ... MBP pulldown assays were performed similar to GST pull down assays using Amylose resin (New England Biolabs) and 1 nmol of MBP-Oskar 292-325 and 5 nmol of Smaug 37-281.
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bioRxiv - Systems Biology 2021Quote: ... Sequencing libraries were generated from the qualified DNA samples(s) using the NEB Next Ultra DNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2021Quote: ... Total RNA was mixed with Reaction Mix 1 (9:1 of 12 uM NEB RT random 6N-S to 12 uM oligo-dT(20), 12uM dNTPs ...
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bioRxiv - Genomics 2022Quote: ... 10 min) and resuspended in 400uL Buffer M supplemented with 1 mM S-adenosyl-methionine (SAM, New England Biolabs) and 200uL aliquoted as a non-methylated control ...
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bioRxiv - Cell Biology 2022Quote: INS-1 832/3 EV and g1-2 cells transiently expressing the SNAP-GLP-1R were labelled at 37°C with 1 μM of SNAP-Surface 649 fluorescent substrate (S9159S, New England Biolabs) in full media prior to treatment with 100 nM exendin-4 or vehicle for 3 hours ...
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bioRxiv - Microbiology 2020Quote: ... 20 s annealing at the temperature given by NEB TM calculator (https://tmcalculator.neb.com) ...
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bioRxiv - Molecular Biology 2019Quote: ... and 640 µM S-adenosylmethionine (SAM) (New England Biolabs). The reaction was incubated for 5 min at 37 °C and placed on ice until grids were prepared.
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bioRxiv - Genomics 2019Quote: ... 96 μM S-adenosylmethionine (SAM; New England Biolabs, NEB), and 200 U M ...
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bioRxiv - Genomics 2019Quote: ... 96 μM S-adenosylmethionine (SAM; New England Biolabs, NEB), and 200 U M ...