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Citations for New England Biolabs :
251 - 300 of 1435 citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... magnetic chitin resin (NEB #E8036S) was prepared by washing three times in PBS ...
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bioRxiv - Genetics 2020Quote: ... mRNA was prepared from total RNA by magnetic mRNA purification (Magnetic mRNA Isolation Kit,NEB) according to the manufacturer’s’ instructions with the following deviation ...
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bioRxiv - Microbiology 2021Quote: ... MNase activity was quenched by addition of EGTA and samples were subsequently pre-cleared by addition of 40 µl Protein A Magnetic Beads (New England Biolabs, Ipswich, MA, United States) and rotating at 4°C for 60 min ...
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bioRxiv - Microbiology 2020Quote: ... samples were treated using a Pan-Prokaryote riboPOOL™ kit (siTOOLs Biotech Gmbh, Planegg, Germany) coupled with streptavidin magnetic beads (New England Biolabs, Ipswich, MA, USA). mRNA was eluted in 50 μl of nuclease-free water and then cleaned and concentrated using a Clean & Concentrator kit (Zymo Research ...
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bioRxiv - Cell Biology 2019Quote: ... the purified C-I30-Flag protein (30µl beads) was digested on beads (Anti-FLAG M2 affinity gel) with final 5U Enterokinase (cat#: P8070S, NEB) in the 1x EK reaction buffer (20 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... the purified C-I30-Flag protein (30µl beads) was digested on beads (Anti-FLAG M2 affinity gel) with final 10U CIP Phosphatase (cat#: M0290S, NEB) in the 1x CIP buffer (50 mM Potassium Acetate ...
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bioRxiv - Developmental Biology 2023Quote: ... and Magnetic Separation Rack (NEB S1507S) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Supernatants were separated from lysates and incubated with affinity beads at 4°C overnight (His beads: Ni-NTA from QIAGEN; GST beads: glutathione-agarose beads from ROTH; MBP beads: amylose beads from NEB). Protein-binding beads were collected by centrifugation and washed several times with washing buffer (His washing buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse α-myc (New England Biolabs, clone 9B11, 1:2,000), rabbit α-pol-I largest subunit 15 (1:100 ...
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bioRxiv - Genetics 2022Quote: ... and PolyA mRNA magnetic isolation module (NEB). Sequencing was performed using NextSeq 500/550 High-Output v2.5 (150 cycle ...
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bioRxiv - Microbiology 2021Quote: ... Chitin magnetic particles (New England Biolabs, #E8036L) were washed in sterile artificial seawater (Sigma-Aldrich ...
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bioRxiv - Synthetic Biology 2023Quote: ... The expression of each engineered component was validated pre- and post-sort by staining Jurkat cells with an anti-Myc antibody (#2233S, NEB, MA, USA), followed by performing flow cytometry on a FACSCanto (BD Biosciences) ...
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bioRxiv - Physiology 2022Quote: ... The pCMV-myc plasmid was digested with EcoRI (New England Biolabs). The PCR products and digested plasmids were electrophoresed ...
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bioRxiv - Cell Biology 2023Quote: ... the cell–ConA bead complexes were incubated with anti- H3K9la (PTM BIOLABS, PTM1419RM) at 4°C overnight and with a secondary antibody for 1 h at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... samples were enriched for mRNA using oligo-dT binding and magnetic separation using the NEBNext Poly(A) Magnetic Isolation Module (New England Biolabs). Samples were reverse transcribed using the NEBNext RNA First and Second Strand Synthesis Modules (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The NEBNext magnetic isolation module (New England Biolabs), was used for mRNA enrichment ...
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bioRxiv - Microbiology 2020Quote: ... with poly(A) Magnetic Isolation Module (NEB#7490). Fragmentation was carried out using divalent cations under elevated temperature in NEBNext first strand synthesis reaction buffer ...
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bioRxiv - Molecular Biology 2022Quote: ... and Poly-A mRNA magnetic isolation module (NEB). The libraries were sequenced on Illumina NextSeq 550 or Illumina NextSeq 2000 instruments ...
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bioRxiv - Genetics 2022Quote: ... with Poly-A mRNA magnetic isolation (NEB #E7490) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2019Quote: ... NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) was used for RNA Isolation ...
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bioRxiv - Molecular Biology 2021Quote: ... The NEBNext Poly(A) mRNA magnetic Isolation Module (NEB) was used to isolate poly(A ...
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bioRxiv - Evolutionary Biology 2019Quote: ... NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) was used for RNA Isolation ...
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bioRxiv - Molecular Biology 2022Quote: ... Complexes were placed in a magnetic separation rack (NEB) to collect the unbound sample and then washed four times with NT2 buffer ...
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bioRxiv - Bioengineering 2022Quote: ... NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB E7490L), NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Systems Biology 2021Quote: ... NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB E7490L), NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB E7770L) ...
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Co-stimulatory molecules decide T cell fate through regulations of their invigoration and impairmentbioRxiv - Molecular Biology 2022Quote: ... with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB), according to manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490L), NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB #E7770L) ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were separated on magnetic streptavidin resin (NEB) and eluted with 2× SDS loading buffer supplemented with 4% SDS and 10 mM Β-mercaptoethanol ...
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bioRxiv - Molecular Biology 2023Quote: ... with the PolyA mRNA magnetic isolation module (#E7490, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... NEB Next Poly(A) mRNA Magnetic Isolation Module (NEB) kit was used to enrich the poly(A ...
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bioRxiv - Immunology 2024Quote: ... Poly(A) mRNA Magnetic Isolation Module (New England BioLabs) was used for cDNA library preparation according to manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB#7490) and NEBNext Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs ...
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bioRxiv - Cell Biology 2021Quote: Myc- and DDK (FLAG)-tagged Hemopexin mRNA was transcribed from an AgeI (NEB) linearised plasmid using T7 RNA polymerase (Promega) ...
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bioRxiv - Neuroscience 2020Quote: ... Streptavidin beads (NEB) were washed twice and blocked in pulldown buffer (Tris-HCl 50mM pH 7.4 ...
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bioRxiv - Cancer Biology 2022Quote: Streptavidin beads (NEB) were washed and loaded with unmodified or T108ph-modified peptides ...
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bioRxiv - Molecular Biology 2023Quote: ... Amylose beads (NEB) were mixed with lysate supernatant for 1.5 h and washed with buffer A ...
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bioRxiv - Molecular Biology 2021Quote: ... the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, E7490L) was used to capture and remove polyA+ mRNA ...
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bioRxiv - Genomics 2020Quote: ... The supernatant was collected using a magnetic rack (NEB S1506S) and neutralized in 10 μl of 1 M Tris pH 8.0 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Using the NEBNext Poly(A) Magnetic mRNA Isolation Kit (NEB), mRNA was isolated from intact total RNA ...
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bioRxiv - Developmental Biology 2021Quote: ... with the NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) to enrich for poly-A tailed RNA molecules ...
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bioRxiv - Microbiology 2021Quote: ... mRNAs pull-up was performed using Magnetic Isolation Module (NEB). All libraries were mixed into a single tube with equal molarity ...
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bioRxiv - Molecular Biology 2022Quote: ... with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490L). cDNA libraries were sequenced by the Sequencing and Genomic Technologies Shared Resource at Duke University on an Illumina NovaSeq6000 using a S-prime or S1 flow cell with paired-end 250- or 150-bp reads respectively ...
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bioRxiv - Systems Biology 2023Quote: ... and NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB, #E7490) following the manufacturer’s specifications using the following options ...
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bioRxiv - Genetics 2023Quote: ... with NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490, NEB) and NEBNext Multiplex Oligos for Illumina (96 Unique index primer pairs ...
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bioRxiv - Molecular Biology 2023Quote: ... then placed on an NEBNext Magnetic Separation Rack (NEB S1515S) for 5 min ...
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bioRxiv - Molecular Biology 2023Quote: ... and placed on an NEBNext Magnetic Separation Rack (NEB S1515S) until the solution appeared clear ...
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bioRxiv - Genomics 2023Quote: ... The NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490) was used to enrich for polyA mRNA ...
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bioRxiv - Genetics 2021Quote: ... and sub-cloned into pcDNA3.1 myc/HisA vector with HiFi cloning (New England Biolabs). We also introduced an endogenous Kozak sequence prior to the start site ...
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bioRxiv - Microbiology 2024Quote: ... The myc epitope was added before the stop codon of blaSHV-5 by NEB Q5 Site-Directed Mutagenesis according to the manufacturer using primers JCP505/506 with pTOX5 blaSHV-5 template ...
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bioRxiv - Molecular Biology 2021Quote: ... pH 8.0) were incubated with pre-washed pan anti-Kbhb beads (PTM Biolabs Inc., Chicago, IL) at 4 °C overnight with gentle shaking ...