Labshake search
Citations for New England Biolabs :
151 - 200 of 1435 citations for Anti Myc magnetic beads since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: RNA-protein complexes were isolated with oligo[dT)]25 magnetic beads (New England Biolabs, S1419S). Briefly ...
-
bioRxiv - Plant Biology 2023Quote: ... Total mRNAs were isolated using biotinylated oligo(dT) and streptavidin magnetic beads (New England Biolabs) as previously described (Wang et al ...
-
bioRxiv - Microbiology 2023Quote: ... μL supernatant were incubated with biotinylated DNA that was immobilized to streptavidin magnetic beads (1 pmol DNA to 1 μL beads; New England Biolabs, Ipswich, MA), as described in Meinhardt et al.79 ...
-
bioRxiv - Molecular Biology 2020Quote: ... polyadenylated RNA was purified by two rounds of binding to Oligo (dT)25 magnetic beads (NEB) and mRNA was fragmented with RNA fragmentation solution (Ambion ...
-
bioRxiv - Developmental Biology 2021Quote: ... and the protease enzyme was further removed from hTNPO1 protein by Ni-NTA Magnetic Beads (NEB) per the manufacturer’s instructions ...
-
bioRxiv - Genomics 2020Quote: ... Further processing for mRNA selection was performed with Oligo-d(T)25 Magnetic Beads (NEB, E7490) and the integrity of the RNA was validated once more with Agilent 4200 TapeStation (Agilent ...
-
bioRxiv - Genomics 2021Quote: ... the rest was subjected to pull-down with oligo-d(T)x25 magnetics beads (NEB-S1419S), 1ml of slurry per 1L of cell culture during 1 hour at 4°C ...
-
bioRxiv - Genomics 2021Quote: ... The remaining RNA was mixed with an equal volume of hydrophilic streptavidin magnetic beads (NEB S1421) which had been pre-washed two times and resuspended in 2M NaCl ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were collected and lysed in RIPA lysis buffer for streptavidin magnetic beads (New England Biolabs) pull down ...
-
bioRxiv - Biophysics 2021Quote: ... which were each applied to ∼400 µL of magnetic Ni-NTA beads (NEB, product number S1423S), equilibrated at 4 °C in binding buffer (50 mM sodium phosphate ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... the lysate was pre-cleared as follows: 100 μl of streptavidin magnetic beads (New England Biolabs) were washed in 1 ml of 1×PBS for 3 times and the FLAG-tagged Hyl1La lysate was added to the washed beads ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... the lysate was pre-cleared as following: 100 μl of streptavidin magnetic beads (New England Biolabs) were washed in 1 ml of 1×PBS for 3 times and the FLAG-tagged TBP::NveRLR/wild-type lysate was added to the washed beads ...
-
bioRxiv - Molecular Biology 2019Quote: ... The lysate was then incubated with 200 μL of oligo-dT 25 Magnetic Beads (NEB, S1419S) for 10 minutes ...
-
bioRxiv - Cell Biology 2021Quote: ... mRNAs were isolated from total RNAs with Magnetic mRNA Isolation Kit (oligo(dT) beads) (NEB, E7490). First strand cDNAs were synthesized using random primers and second strand cDNAs were synthesized subsequently ...
-
bioRxiv - Molecular Biology 2021Quote: ... The antibodies were captured using 50 µl of protein G magnetic beads (S1430, New England Biolabs) incubated for 60 min with end over end mixing at 4°C ...
-
bioRxiv - Cell Biology 2021Quote: ... Lis1 was first covalently coupled to 16 μL of SNAP-Capture Magnetic Beads (New England Biolabs) in 2 mL Protein Lo Bind Tubes (Eppendorf ...
-
bioRxiv - Genomics 2022Quote: ... polyadenylated RNA was isolated from total RNA using oligo d(T)25 Magnetic Beads (NEB, #S1419S) at a 1μg Total RNA to 10μg beads ratio ...
-
bioRxiv - Molecular Biology 2022Quote: ... prior to primary transcript enrichment of the former using Hydrophilic Streptavidin magnetic beads (New England Biolabs). RNA from both samples was then reverse transcribed and PCR amplified according to the PCR-cDNA barcoding protocol (SQK-PCB109 ...
-
bioRxiv - Neuroscience 2023Quote: ... The lysate-antibody mix was then incubated with Magnetic Protein A beads (New England Biolabs, S1425S) overnight at 4 °C with end over rotation ...
-
bioRxiv - Microbiology 2023Quote: ... encoding a C-terminal myc tag were cloned into NcoI and HindIII sites of pHERD20T-myc and pHERD30T-myc using Gibson Assembly (NEB) or Hifi Assembly (NEB) ...
-
bioRxiv - Genomics 2022Quote: ... and mRNA was selected using 75 μl of Oligo d(T)25 magnetic beads (New England Biolabs) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2022Quote: ... The lysate (input) was then incubated with 200 μl oligo(dT)25 magnetic beads (New England Biolabs) for 1 h at 4°C on a rotator ...
-
bioRxiv - Genetics 2021Quote: ... The ligation reaction was purified by magnetic beads and digested with 40 units of XcmI (#R0533S; NEB) for 3 h ...
-
bioRxiv - Genomics 2021Quote: ... mRNA was isolated from 100 ng of total RNA per sample using oligo dT magnetic beads (NEB) according to the manufacturer’s protocol ...
-
bioRxiv - Molecular Biology 2022Quote: For chitin-based sandwich ELISA 50 μl of chitin magnetic beads (New England Biolabs, Ipswich, MA, USA) were transferred into a 1.5 ml reaction tube ...
-
bioRxiv - Cell Biology 2023Quote: ... myc-tagged AP3B1 immunoprecipitated and immobilised on Protein G-sepharose beads was incubated with CK2 (250 units; New England Biolabs) for 30 min ...
-
bioRxiv - Molecular Biology 2020Quote: ... and excess adaptors were removed by magnetic beads (HighPrep PCR, Magbio) before and after amplification (Phusion polymerase, NEB).
-
bioRxiv - Genomics 2020Quote: ... Samples were pre-cleared by adding 40 µl Protein A Magnetic Beads (New England Biolabs, MA, United States) and rotating at 4°C for 60 min ...
-
bioRxiv - Cell Biology 2019Quote: ... Lysates containing 800μg of protein in a total of 400μl lysis buffer were pre-cleared by incubation with 15μl of Protein A magnetic beads (New England Biolabs) for 1h at 4°C under agitation ...
-
bioRxiv - Cell Biology 2021Quote: ... The cleaved 6xHis-MBP tag was removed by incubating the solution with Ni-NTA magnetic beads (NEB; S1423) for 1 hour at 4 °C ...
-
bioRxiv - Biochemistry 2022Quote: ... The mixture was then added to 100 µL of NEBNext® Sample Purification Magnetic Beads (New England Biolabs) and incubated at room temperature for 5 min ...
-
bioRxiv - Molecular Biology 2020Quote: Biotinylated DNA pellets were re suspended in 25μl TNE0.2 buffer (200mM NaCl, 10mMTris-HCl 7.5, 1mM EDTA) and mixed with 25μl Streptavidin coated magnetic beads (NEB, pre washed in TNE0.2 and blocked with 100μg/ml salmon sperm DNA) ...
-
bioRxiv - Cell Biology 2021Quote: ... 5 µg antibody (Table. S3) and 50 µl protein A magnetic beads (New England Biolabs, Cat. No. S1425S) were added to the supernatant and incubate overnight at 4 degree Celsius ...
-
bioRxiv - Cancer Biology 2019Quote: ... while single-timepoint experiments assessing splicing required mRNA enrichment with magnetic mRNA Isolation kit poly-dT beads (NEB), then RNA Hyper Prep kit (Kapa ...
-
bioRxiv - Neuroscience 2020Quote: ... Poly(A)+ RNAs were enriched from total RNAs using Oligo d(T)25 magnetic beads (New England Biolabs), according to the manufacturer’ s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... A small fraction of the refolded MBP-ACE2NTD fusion protein was further purified by amylose magnetic beads (NEB) according to the protocol provided by the manufacturer.
-
bioRxiv - Molecular Biology 2022Quote: ... The poly(A) RNA was prepared using two rounds of oligo(dT) magnetic beads purification (New England Biolabs).
-
bioRxiv - Molecular Biology 2022Quote: ... This was followed by one round poly(A) purification using oligo d(T) magnetic beads (New England Biolabs). 1.5-2 µg of mRNA was fragmented to 100-150 nts using RNA Fragmentation Reagent (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1 mg of cell lysate was cleared with protein A magnetic beads to remove non-specific interactions (S1425S, NEB) and incubated overnight with anti-ZAP antibody (Proteintech 16820-1-AP ...
-
bioRxiv - Molecular Biology 2019Quote: ... Poly(A) RNA was isolated using one round of selection with oligo(dT)25 magnetic beads (New England Biolabs). This resulted in approximately 10 μ g of poly(A ...
-
bioRxiv - Cancer Biology 2020Quote: ... PolyA-tailed mRNA was selected with beads from 1μg total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). cDNAs were generated using random hexamers and ligated to barcoded Illumina adaptors with the NEXTflex Rapid Directional RNA-Seq Library Prep Kit (Bioo Scientific) ...
-
bioRxiv - Immunology 2021Quote: ... recombinant HAΔTM H1N1 A/Michigan/45/2015 and H3ΔTM H3N2 A/Hong Kong/4801/2014 were biotinylated and coupled to streptavidin magnetic beads (New England Biolabs) at a ratio of 2.5 μg of protein to 25 μl of beads ...
-
bioRxiv - Cell Biology 2020Quote: The remainder of the protein was incubated with 50 μl of Ni-NTA Magnetic Beads (New England Biolabs, Inc.) on a rocker for 45 min at room temperature ...
-
bioRxiv - Genomics 2022Quote: ... The particles-tethered-magnetic-beads (hereafter referred to as ‘P_beads’) were then either digested with 1000 U of HindIII-HF (NEB) in 1 ml of WB1 at 37 °C with mild rotation for over 6 hrs ...
-
bioRxiv - Microbiology 2021Quote: ... Then homogenized lysate incubated with 0.80 mL of pre- equilibrated oligo(dT)25 magnetic beads (New England Biolabs, #S1419S) for 1 h at 4°C with gentle rotation ...
-
bioRxiv - Molecular Biology 2019Quote: ... we incubated the Pol II EC(CPD) (with and without biotin crosslinking) with 2μl streptavidin magnetic beads (NEB, USA) for 30 min at room temperature ...
-
bioRxiv - Systems Biology 2023Quote: ... Linear barcoded vectors were purified by magnetic beads and digested for 3h with 40 units of XcmI (#R0533S; NEB). Finally barcoded vectors were purified again by gel-purification.
-
bioRxiv - Genomics 2023Quote: ... The poly-A+ fraction of RNA was isolated using Oligo d(T)25 Magnetic Beads (New England BioLabs Inc.) following the commercial protocol for Mammalian Cells provided by the manufacturer ...
-
bioRxiv - Molecular Biology 2023Quote: ... ∼40 µg of bulk RNA was subjected to poly(A) selection using Oligo d(T)25 magnetic beads (NEB) followed by on-bead 5’ ligation of a biotinylated REL5 linker sequence using T4 RNA ligase 1 (NEB ...
-
bioRxiv - Plant Biology 2023Quote: ... The precipitates were eluted into 30μL 1×SDS loading buffer and detected using anti-Myc and anti-MBP antibody (1:5,000, New England Biolabs).